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The map label for this gene is purF [H]

Identifier: 116493199

GI number: 116493199

Start: 1434785

End: 1436254

Strand: Reverse

Name: purF [H]

Synonym: PEPE_1461

Alternate gene names: 116493199

Gene position: 1436254-1434785 (Counterclockwise)

Preceding gene: 116493200

Following gene: 116493198

Centisome position: 78.38

GC content: 40.61

Gene sequence:

>1470_bases
ATGGCAGCTGAATTAAAGGGTCTAAACGAAGAATGCGGTGTTTTTGGTATTTGGAATGATTCAGAAGCTGCCCAACGGAC
CTACTTCGGACTTCACAGCTTACAACATCGAGGCCAAGAAGGTGCTGGTATTGTCGTTCGTAATCAAGAAGGACAGTTGA
AAATTCATAAAGGAAAGGGGCTTTTGGCAGATGTATTTAGATGTCCCGATGATTTGGCACAGCTAATTGGGCCGAGTGCG
ATTGGTCATGTGCGCTATGGAACAGCTGGAAACCGAGGAATTGAAAATATCCAACCGTTACTTTTCCACTACTTACAGGG
GGATGTGGCGATGGCCCATAATGGTAATTTAACCAATGCAGTTAGTTTACGACGCGAATTAGAAAAAAGTGGTGCTACTT
TTCAATCTAATTCTGATACGGAAGTACTAATGCATTTAATTCAGCATCAAAAAGGTCCAACTTTTATTGAAAAATTAAAA
GCGAGTTTAAAACAAATTCAAGGTGGATTTGCTTACTTAATCCTTACCAAAGATGCACTGTACGCGGCCCTTGATCCGAA
TGGTTTTCGGCCCTTAGTAATTGGAAAATTAGCTAACGGTTCGTATGTTGTTGCTAGTGAAACCTGTGCTTTAGATGCGG
TCGGAGCAACCTTGGTTCGAAATGTTGAACCGGGACAGTTAGTTCAAATTGATCATCATGGGCTCCAAATTAGTAATTTT
ACTACTAAAACCCAGTTATCAATTTGTTCGATGGAATACATTTATTTTGCGCGACCCGATTCAAATCTTTATGGCGTTAA
TGTTCATCTCGCTCGTCAACGGATGGGTCAACGATTGGCAAAAGAAAACCCCATTAAGGTCGATATGGTGGTGGGGGTGC
CGAACTCTTCGTTATCAGCAGCATCTGGAGTGGCCGAACAACTAGGCGTTCCCAATGAAATGGGATTAATTAAAAATCAA
TATAGTAGTAGGACTTTTATTCAACCGACCCAAGCACTACGTGAACAGGGAGTGAAGATGAAATTATCTGCATTAAAAGC
AGTAATTGCGGGGAAAGATGTGGCATTAGTTGACGATTCAATCGTGCGGGGTACCACCAGTCAGTATATTGTGAAAATGT
TAAAAAATGCAGGAGCTAAGTCGGTGCATTTGATGATAAGTTCTCCACCACTTAAATATCCATGTTTTTATGGAATTGAT
ATTGAACATACACGTGAATTGATGGCTGCTAATTATACGGTAAGTGAGATGTGTCAGAAAATTGGAGCAGATTCACTGCA
TTTTTTGAGCCTGCCAGGTTTAATTGAGGCGATTGGTTTACATCAAGATGCCCCTAATGATGGCTTATGTGTAGCGTATT
TTGATGGTAAATATCCCACCCCCCTTTATGATTATGAAAAAGATTTTGAGATCCCTAAAACAGCTAACGACTTTACATTA
TCAGCAATGGCCCAAGTGGAGGAAAAATAA

Upstream 100 bases:

>100_bases
TCGGCCAAGTTGGCGGTAAGCGGATCAAAATTGCAACGATGGATGATGTAATTGATGTTGATTTGAAAAATACTAAGGAG
AGTTGGAAGGGAGCCCTAGC

Downstream 100 bases:

>100_bases
TGGTAGAAAGTGCTTATGAAAAAGCAGGTGTGAATATTACAGCTGGTGAAGAACTTGTAGCTGATCTAAAGCAAAAATTA
GGTGCTCCAGACCCTCATGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MAAELKGLNEECGVFGIWNDSEAAQRTYFGLHSLQHRGQEGAGIVVRNQEGQLKIHKGKGLLADVFRCPDDLAQLIGPSA
IGHVRYGTAGNRGIENIQPLLFHYLQGDVAMAHNGNLTNAVSLRRELEKSGATFQSNSDTEVLMHLIQHQKGPTFIEKLK
ASLKQIQGGFAYLILTKDALYAALDPNGFRPLVIGKLANGSYVVASETCALDAVGATLVRNVEPGQLVQIDHHGLQISNF
TTKTQLSICSMEYIYFARPDSNLYGVNVHLARQRMGQRLAKENPIKVDMVVGVPNSSLSAASGVAEQLGVPNEMGLIKNQ
YSSRTFIQPTQALREQGVKMKLSALKAVIAGKDVALVDDSIVRGTTSQYIVKMLKNAGAKSVHLMISSPPLKYPCFYGID
IEHTRELMAANYTVSEMCQKIGADSLHFLSLPGLIEAIGLHQDAPNDGLCVAYFDGKYPTPLYDYEKDFEIPKTANDFTL
SAMAQVEEK

Sequences:

>Translated_489_residues
MAAELKGLNEECGVFGIWNDSEAAQRTYFGLHSLQHRGQEGAGIVVRNQEGQLKIHKGKGLLADVFRCPDDLAQLIGPSA
IGHVRYGTAGNRGIENIQPLLFHYLQGDVAMAHNGNLTNAVSLRRELEKSGATFQSNSDTEVLMHLIQHQKGPTFIEKLK
ASLKQIQGGFAYLILTKDALYAALDPNGFRPLVIGKLANGSYVVASETCALDAVGATLVRNVEPGQLVQIDHHGLQISNF
TTKTQLSICSMEYIYFARPDSNLYGVNVHLARQRMGQRLAKENPIKVDMVVGVPNSSLSAASGVAEQLGVPNEMGLIKNQ
YSSRTFIQPTQALREQGVKMKLSALKAVIAGKDVALVDDSIVRGTTSQYIVKMLKNAGAKSVHLMISSPPLKYPCFYGID
IEHTRELMAANYTVSEMCQKIGADSLHFLSLPGLIEAIGLHQDAPNDGLCVAYFDGKYPTPLYDYEKDFEIPKTANDFTL
SAMAQVEEK
>Mature_488_residues
AAELKGLNEECGVFGIWNDSEAAQRTYFGLHSLQHRGQEGAGIVVRNQEGQLKIHKGKGLLADVFRCPDDLAQLIGPSAI
GHVRYGTAGNRGIENIQPLLFHYLQGDVAMAHNGNLTNAVSLRRELEKSGATFQSNSDTEVLMHLIQHQKGPTFIEKLKA
SLKQIQGGFAYLILTKDALYAALDPNGFRPLVIGKLANGSYVVASETCALDAVGATLVRNVEPGQLVQIDHHGLQISNFT
TKTQLSICSMEYIYFARPDSNLYGVNVHLARQRMGQRLAKENPIKVDMVVGVPNSSLSAASGVAEQLGVPNEMGLIKNQY
SSRTFIQPTQALREQGVKMKLSALKAVIAGKDVALVDDSIVRGTTSQYIVKMLKNAGAKSVHLMISSPPLKYPCFYGIDI
EHTRELMAANYTVSEMCQKIGADSLHFLSLPGLIEAIGLHQDAPNDGLCVAYFDGKYPTPLYDYEKDFEIPKTANDFTLS
AMAQVEEK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=516, Percent_Identity=37.5968992248062, Blast_Score=332, Evalue=7e-91,
Organism=Escherichia coli, GI1788651, Length=474, Percent_Identity=37.1308016877637, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1790167, Length=235, Percent_Identity=29.3617021276596, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=38.8308977035491, Blast_Score=322, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6323958, Length=482, Percent_Identity=34.8547717842324, Blast_Score=243, Evalue=5e-65,
Organism=Drosophila melanogaster, GI28573187, Length=501, Percent_Identity=38.9221556886228, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24659598, Length=488, Percent_Identity=37.5, Blast_Score=328, Evalue=4e-90,
Organism=Drosophila melanogaster, GI24659604, Length=460, Percent_Identity=38.0434782608696, Blast_Score=322, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 53365; Mature: 53234

Theoretical pI: Translated: 7.06; Mature: 7.06

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAELKGLNEECGVFGIWNDSEAAQRTYFGLHSLQHRGQEGAGIVVRNQEGQLKIHKGKG
CCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCC
LLADVFRCPDDLAQLIGPSAIGHVRYGTAGNRGIENIQPLLFHYLQGDVAMAHNGNLTNA
HHHHHHCCCHHHHHHHCCCHHCCEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHH
VSLRRELEKSGATFQSNSDTEVLMHLIQHQKGPTFIEKLKASLKQIQGGFAYLILTKDAL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCE
YAALDPNGFRPLVIGKLANGSYVVASETCALDAVGATLVRNVEPGQLVQIDHHGLQISNF
EEEECCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCEEEECC
TTKTQLSICSMEYIYFARPDSNLYGVNVHLARQRMGQRLAKENPIKVDMVVGVPNSSLSA
CCCCEEEEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
ASGVAEQLGVPNEMGLIKNQYSSRTFIQPTQALREQGVKMKLSALKAVIAGKDVALVDDS
HHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHCCCEEEHHHHHHHHCCCCEEEECCH
IVRGTTSQYIVKMLKNAGAKSVHLMISSPPLKYPCFYGIDIEHTRELMAANYTVSEMCQK
HHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHH
IGADSLHFLSLPGLIEAIGLHQDAPNDGLCVAYFDGKYPTPLYDYEKDFEIPKTANDFTL
HCCCCEEEEECCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
SAMAQVEEK
HHHHHHCCC
>Mature Secondary Structure 
AAELKGLNEECGVFGIWNDSEAAQRTYFGLHSLQHRGQEGAGIVVRNQEGQLKIHKGKG
CCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCC
LLADVFRCPDDLAQLIGPSAIGHVRYGTAGNRGIENIQPLLFHYLQGDVAMAHNGNLTNA
HHHHHHCCCHHHHHHHCCCHHCCEEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHH
VSLRRELEKSGATFQSNSDTEVLMHLIQHQKGPTFIEKLKASLKQIQGGFAYLILTKDAL
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCE
YAALDPNGFRPLVIGKLANGSYVVASETCALDAVGATLVRNVEPGQLVQIDHHGLQISNF
EEEECCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHHCCCCCCCEEEEECCCEEEECC
TTKTQLSICSMEYIYFARPDSNLYGVNVHLARQRMGQRLAKENPIKVDMVVGVPNSSLSA
CCCCEEEEEEEEEEEEECCCCCEEEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHH
ASGVAEQLGVPNEMGLIKNQYSSRTFIQPTQALREQGVKMKLSALKAVIAGKDVALVDDS
HHHHHHHHCCCCHHHHHHHHCCCCCEECHHHHHHHCCCEEEHHHHHHHHCCCCEEEECCH
IVRGTTSQYIVKMLKNAGAKSVHLMISSPPLKYPCFYGIDIEHTRELMAANYTVSEMCQK
HHCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCHHHHHHHHHCCCCHHHHHHH
IGADSLHFLSLPGLIEAIGLHQDAPNDGLCVAYFDGKYPTPLYDYEKDFEIPKTANDFTL
HCCCCEEEEECCHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHH
SAMAQVEEK
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA