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The map label for this gene is pcrA [H]

Identifier: 116493117

GI number: 116493117

Start: 1360035

End: 1362308

Strand: Reverse

Name: pcrA [H]

Synonym: PEPE_1372

Alternate gene names: 116493117

Gene position: 1362308-1360035 (Counterclockwise)

Preceding gene: 116493118

Following gene: 116493116

Centisome position: 74.35

GC content: 37.55

Gene sequence:

>2274_bases
TTGCCAGAAATTAATTTAATAAAAGGCCTTAATGATATGCAAAAAGAAGCGGTTCAGACAACTGAAGGACCGTTATTGAT
TATGGCCGGCGCCGGTAGTGGTAAGACCCGAGTTTTGACGCACCGCGTTGCATATCTAATAGAAGAAAAAGGTGTTAATC
CGTGGAATGTTTTGGCAATTACCTTCACTAATAAGGCTGCTAGAGAGATGCGTGAGCGAGTTACTAGTTTGCTGGGTGAT
GAAGCTGCTGAAATTTGGGTATCAACGTTCCATGCTCTTTGCGTAAGAATTTTAAGAAGAAATATTGATCAACTTGGATA
TAATCGAGCATTTTCGATTGCCGATCCTAGCGAACAAAGAACCCTAGTTAAACATATTTTAGCCGATTTAAATTATGATC
CTAAAAAGTTTGATCCACGTTCAGTTTTAGGAACGATTTCCAATGCTAAAAATGATTTAAAAACGCCACTCACTTATCGT
GAGAATGCAAGTACTCCATATGAACAGGTAGTCGCAGATATCTATGAAGAATATCAGCGTCGCTTAGAGAACAATCAGGC
TTTAGATTTTGATGATTTGATTATGAAAACCATCGAACTTTTCCAAAAGAATCCTGAAACCTTATCATTTTATCAAAATA
AATTTCATTATATTCATGTGGATGAATATCAGGATACTAATCAGGCTCAATATCAATTAGTAACGATGCTAGCTAAACAT
TTTAGAAATATCTGTGTAGTTGGTGATTCTGATCAAAGTATTTATGGTTGGCGGGGCGCCGATATGCAAAACATCATGAA
CTTTGAATCAGATTATCCAGATTCAAAGACTGTAATGTTGGAACAAAACTATCGTTCAACCCAAAATATTTTAAATGCTG
CTAATGATGTGATTGAAAATAATGTAGAACGTAAACCTAAACAACTTTGGACTGATAATGATGAAGGTGAAAAAATTTCA
TATTATCGTGGCCAGACGGGGATTGATGAAGCCCATTTCATCGTTCAAAAAATTCGTGAGCAAATTAAAGAGCATAATTT
AAATTACGGAGATTTTGCCATTCTTTACCGGACGAATGCACAGTCACGAGTAATTGAAGATACGTTTGTTAAATCCAACA
TCCCTTACTTAATGGTCGGGGGACATAAATTCTACGATCGTAAGGAAATTAGAGATGTACTGAGTTACCTTACTTTGATT
GCTAACGAAGACGATTCGATGAGTTTTGAACGAGTGATCAATGAACCTAAACGTGGGATTGGTTCGACTTCTATTGCTAA
ATTACAAGCTTTCGCGGATGAAAATAATTGGTCCTTATTGAAAGCGGCTCGTAGTGTTCAGATTGCAAATGGAATTTCTA
GTCGAGCTCGCAATGCAATTGAAGATTTCGCTAAGATGATTGACGCTCTACGTAAATCAGGTGAATTTTTAAATATCACA
GAATTAACAGAAGCAGTCTTGGAACAAACTGGTTATCGAGCTGCTTTAGAAAAATCTAGAACGATGGAATCAGAAGCACG
CCTTGAAAATATTGACGAATTTTTAACAGTTACTAAGCAATTTGACGATAAGAATGAAGCTGAGGGAAATGAAATTGCTG
ACGAAAATGGTGATAACATTCAATTAGTAGATTTCCTAGCGGATTTGGCCTTAGTTTCTGATCAAGATAATGTGGAAGAA
GAACCATCAGGAGTAACCCTCATGACCCTGCATGCGGCTAAGGGTCTTGAATTTCCAATCGTATTTTTAGTTGGGATGGA
AGATGGAATTTTCCCACTTTCTAGAGCAATGATGGACCAAGAACAATTGGAAGAAGAACGACGATTAGCCTATGTTGGAA
TCACCCGAGCACGTCAAAAGTTGTATATTACGAATGCTTATTCTAGAATGCTTTATGGTAGGACACAAACTAATCCAGCT
TCACGGTTCATTTCAGAAATTGGATCACAATATTTGAAGAGTGAAAATGAAGAAAAGAGTTTTTCAAGAAATACTAAAGA
AAAATCATATCCGTTTGACAATAACCGAAATGCACGCGCAACAGCTAGTGTTTATGGTAATACCCATGCTACGGCGGTTA
AATCTAATGGTAGCGGAGCTGGTAAAGTTAGCTGGAATGCTGGGGATAAAGTCGAACATAAAGCTTGGGGTATTGGTACC
GTAGTGAAGGTGTCTGGCGCTGGAGAAGATGCCGAATTGGATATCGCCTTTAAAGATCAAGGAATTAAGCGGTTATTGGC
CGCTTTTGCACCAATTAAACGGGTTGTTGAATAA

Upstream 100 bases:

>100_bases
TTTGGTAAAACTCTGTTAAAGTCGGTAAATAAACCGGCTTTTTTTGATATAATGGTTTGTGGTTATTTTTTAGTTACGTA
GATAGAGAGGATTGAACAAA

Downstream 100 bases:

>100_bases
AGAATTTTATACAGGGTTTAGGTTAGCGGTATAATTAGGGATAAATGAAAGATTGAGGAAACTTTTCTATGAATGTAACT
AAAAAATTGAGTTTATTAGT

Product: ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MPEINLIKGLNDMQKEAVQTTEGPLLIMAGAGSGKTRVLTHRVAYLIEEKGVNPWNVLAITFTNKAAREMRERVTSLLGD
EAAEIWVSTFHALCVRILRRNIDQLGYNRAFSIADPSEQRTLVKHILADLNYDPKKFDPRSVLGTISNAKNDLKTPLTYR
ENASTPYEQVVADIYEEYQRRLENNQALDFDDLIMKTIELFQKNPETLSFYQNKFHYIHVDEYQDTNQAQYQLVTMLAKH
FRNICVVGDSDQSIYGWRGADMQNIMNFESDYPDSKTVMLEQNYRSTQNILNAANDVIENNVERKPKQLWTDNDEGEKIS
YYRGQTGIDEAHFIVQKIREQIKEHNLNYGDFAILYRTNAQSRVIEDTFVKSNIPYLMVGGHKFYDRKEIRDVLSYLTLI
ANEDDSMSFERVINEPKRGIGSTSIAKLQAFADENNWSLLKAARSVQIANGISSRARNAIEDFAKMIDALRKSGEFLNIT
ELTEAVLEQTGYRAALEKSRTMESEARLENIDEFLTVTKQFDDKNEAEGNEIADENGDNIQLVDFLADLALVSDQDNVEE
EPSGVTLMTLHAAKGLEFPIVFLVGMEDGIFPLSRAMMDQEQLEEERRLAYVGITRARQKLYITNAYSRMLYGRTQTNPA
SRFISEIGSQYLKSENEEKSFSRNTKEKSYPFDNNRNARATASVYGNTHATAVKSNGSGAGKVSWNAGDKVEHKAWGIGT
VVKVSGAGEDAELDIAFKDQGIKRLLAAFAPIKRVVE

Sequences:

>Translated_757_residues
MPEINLIKGLNDMQKEAVQTTEGPLLIMAGAGSGKTRVLTHRVAYLIEEKGVNPWNVLAITFTNKAAREMRERVTSLLGD
EAAEIWVSTFHALCVRILRRNIDQLGYNRAFSIADPSEQRTLVKHILADLNYDPKKFDPRSVLGTISNAKNDLKTPLTYR
ENASTPYEQVVADIYEEYQRRLENNQALDFDDLIMKTIELFQKNPETLSFYQNKFHYIHVDEYQDTNQAQYQLVTMLAKH
FRNICVVGDSDQSIYGWRGADMQNIMNFESDYPDSKTVMLEQNYRSTQNILNAANDVIENNVERKPKQLWTDNDEGEKIS
YYRGQTGIDEAHFIVQKIREQIKEHNLNYGDFAILYRTNAQSRVIEDTFVKSNIPYLMVGGHKFYDRKEIRDVLSYLTLI
ANEDDSMSFERVINEPKRGIGSTSIAKLQAFADENNWSLLKAARSVQIANGISSRARNAIEDFAKMIDALRKSGEFLNIT
ELTEAVLEQTGYRAALEKSRTMESEARLENIDEFLTVTKQFDDKNEAEGNEIADENGDNIQLVDFLADLALVSDQDNVEE
EPSGVTLMTLHAAKGLEFPIVFLVGMEDGIFPLSRAMMDQEQLEEERRLAYVGITRARQKLYITNAYSRMLYGRTQTNPA
SRFISEIGSQYLKSENEEKSFSRNTKEKSYPFDNNRNARATASVYGNTHATAVKSNGSGAGKVSWNAGDKVEHKAWGIGT
VVKVSGAGEDAELDIAFKDQGIKRLLAAFAPIKRVVE
>Mature_756_residues
PEINLIKGLNDMQKEAVQTTEGPLLIMAGAGSGKTRVLTHRVAYLIEEKGVNPWNVLAITFTNKAAREMRERVTSLLGDE
AAEIWVSTFHALCVRILRRNIDQLGYNRAFSIADPSEQRTLVKHILADLNYDPKKFDPRSVLGTISNAKNDLKTPLTYRE
NASTPYEQVVADIYEEYQRRLENNQALDFDDLIMKTIELFQKNPETLSFYQNKFHYIHVDEYQDTNQAQYQLVTMLAKHF
RNICVVGDSDQSIYGWRGADMQNIMNFESDYPDSKTVMLEQNYRSTQNILNAANDVIENNVERKPKQLWTDNDEGEKISY
YRGQTGIDEAHFIVQKIREQIKEHNLNYGDFAILYRTNAQSRVIEDTFVKSNIPYLMVGGHKFYDRKEIRDVLSYLTLIA
NEDDSMSFERVINEPKRGIGSTSIAKLQAFADENNWSLLKAARSVQIANGISSRARNAIEDFAKMIDALRKSGEFLNITE
LTEAVLEQTGYRAALEKSRTMESEARLENIDEFLTVTKQFDDKNEAEGNEIADENGDNIQLVDFLADLALVSDQDNVEEE
PSGVTLMTLHAAKGLEFPIVFLVGMEDGIFPLSRAMMDQEQLEEERRLAYVGITRARQKLYITNAYSRMLYGRTQTNPAS
RFISEIGSQYLKSENEEKSFSRNTKEKSYPFDNNRNARATASVYGNTHATAVKSNGSGAGKVSWNAGDKVEHKAWGIGTV
VKVSGAGEDAELDIAFKDQGIKRLLAAFAPIKRVVE

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=750, Percent_Identity=39.4666666666667, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI48994965, Length=659, Percent_Identity=39.1502276176024, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1787196, Length=92, Percent_Identity=42.3913043478261, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322369, Length=753, Percent_Identity=29.3492695883134, Blast_Score=256, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324477, Length=679, Percent_Identity=25.4786450662739, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 85871; Mature: 85740

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEINLIKGLNDMQKEAVQTTEGPLLIMAGAGSGKTRVLTHRVAYLIEEKGVNPWNVLAI
CCCCHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMRERVTSLLGDEAAEIWVSTFHALCVRILRRNIDQLGYNRAFSIADPSEQR
EECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
TLVKHILADLNYDPKKFDPRSVLGTISNAKNDLKTPLTYRENASTPYEQVVADIYEEYQR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHCCCCEECCCCCCCHHHHHHHHHHHHHH
RLENNQALDFDDLIMKTIELFQKNPETLSFYQNKFHYIHVDEYQDTNQAQYQLVTMLAKH
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHH
FRNICVVGDSDQSIYGWRGADMQNIMNFESDYPDSKTVMLEQNYRSTQNILNAANDVIEN
HCCEEEECCCCCCEECCCCCCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
NVERKPKQLWTDNDEGEKISYYRGQTGIDEAHFIVQKIREQIKEHNLNYGDFAILYRTNA
CHHCCCHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QSRVIEDTFVKSNIPYLMVGGHKFYDRKEIRDVLSYLTLIANEDDSMSFERVINEPKRGI
CHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHCCC
GSTSIAKLQAFADENNWSLLKAARSVQIANGISSRARNAIEDFAKMIDALRKSGEFLNIT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
ELTEAVLEQTGYRAALEKSRTMESEARLENIDEFLTVTKQFDDKNEAEGNEIADENGDNI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
QLVDFLADLALVSDQDNVEEEPSGVTLMTLHAAKGLEFPIVFLVGMEDGIFPLSRAMMDQ
EHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCH
EQLEEERRLAYVGITRARQKLYITNAYSRMLYGRTQTNPASRFISEIGSQYLKSENEEKS
HHHHHHHHEEHEEHHHHHHEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
FSRNTKEKSYPFDNNRNARATASVYGNTHATAVKSNGSGAGKVSWNAGDKVEHKAWGIGT
HHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEECCCCCCCCHHHCCCCE
VVKVSGAGEDAELDIAFKDQGIKRLLAAFAPIKRVVE
EEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PEINLIKGLNDMQKEAVQTTEGPLLIMAGAGSGKTRVLTHRVAYLIEEKGVNPWNVLAI
CCCHHHHCCCHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEE
TFTNKAAREMRERVTSLLGDEAAEIWVSTFHALCVRILRRNIDQLGYNRAFSIADPSEQR
EECCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHH
TLVKHILADLNYDPKKFDPRSVLGTISNAKNDLKTPLTYRENASTPYEQVVADIYEEYQR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHCHHHHCCCCEECCCCCCCHHHHHHHHHHHHHH
RLENNQALDFDDLIMKTIELFQKNPETLSFYQNKFHYIHVDEYQDTNQAQYQLVTMLAKH
HHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHH
FRNICVVGDSDQSIYGWRGADMQNIMNFESDYPDSKTVMLEQNYRSTQNILNAANDVIEN
HCCEEEECCCCCCEECCCCCCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
NVERKPKQLWTDNDEGEKISYYRGQTGIDEAHFIVQKIREQIKEHNLNYGDFAILYRTNA
CHHCCCHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
QSRVIEDTFVKSNIPYLMVGGHKFYDRKEIRDVLSYLTLIANEDDSMSFERVINEPKRGI
CHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCHHCCC
GSTSIAKLQAFADENNWSLLKAARSVQIANGISSRARNAIEDFAKMIDALRKSGEFLNIT
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHH
ELTEAVLEQTGYRAALEKSRTMESEARLENIDEFLTVTKQFDDKNEAEGNEIADENGDNI
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCE
QLVDFLADLALVSDQDNVEEEPSGVTLMTLHAAKGLEFPIVFLVGMEDGIFPLSRAMMDQ
EHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCHHHHHHHHCH
EQLEEERRLAYVGITRARQKLYITNAYSRMLYGRTQTNPASRFISEIGSQYLKSENEEKS
HHHHHHHHEEHEEHHHHHHEEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHH
FSRNTKEKSYPFDNNRNARATASVYGNTHATAVKSNGSGAGKVSWNAGDKVEHKAWGIGT
HHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCEEECCCCCCCCHHHCCCCE
VVKVSGAGEDAELDIAFKDQGIKRLLAAFAPIKRVVE
EEEEECCCCCCEEEEEECHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA