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The map label for this gene is yojA [H]

Identifier: 116493106

GI number: 116493106

Start: 1346109

End: 1347461

Strand: Reverse

Name: yojA [H]

Synonym: PEPE_1360

Alternate gene names: 116493106

Gene position: 1347461-1346109 (Counterclockwise)

Preceding gene: 116493107

Following gene: 116493105

Centisome position: 73.54

GC content: 38.58

Gene sequence:

>1353_bases
ATGGAAATTATGGTCTTAGTTATTGGAATAATTTTATTGTTAACTTTAATTATAAAATTTAAGATGAATACTTATGTTTC
ATTGATTATTACTGCGGTGGTTGTTGGATTAGGTTTAGGGATGCCGGCTAGCCAGATTGCCACCACGATTCAAAATGGAA
TTGGGAGTCAATTGGGTGAACTAGCGTTGGTATTTGGCTTTGGAGCTATGTTGGGTCGCTTGGTTGCCGACGCAGGGGGA
GCCTATCGGATTGCTAATACCTTAATCGATAAATTCGGACGCCGCTTCTTACAAATTGCTGTTGTAGTTGCTTCGTTTAT
TATTGGGATTGCACTTTTCTTTGAAGTAGGAATCGTGTTATTAGTACCAATTGTATTCGCAATTGCGATCGAAGCAGGGG
TTCCAGTTCTTTCCTTAGGAATTCCAATGGCAGCAGCCTTATCAGTTACTCACGGATTTTTGCCACCACATCCAGCTCCT
ACTGCAATTGCTGCAGTCCTGAATGCTAATGCTGGAAAGGTTTTACTTTACGGAGTGATTATTGCAATTCCAACTGTTTA
TATTGCAGGTGTATTATTTTCAAAAATTGCTAAAAAATATCTTCCAGAAGCGTTTAAAAGAAATGGTAATTTAAAAGCTT
TAGGACCTCAAAAACAATTTAAATTAGAGGAAACTCCGGGATTCGGTATTTCGGTTTTAACTGCTCTTTTCCCAGTCATC
TTAATGGCTATCACAACGATATATGAATTGATTGTCCACGACGGTGTTACGCCTAAAAATCCTAATGCGGTTGACAATTT
TTTTGGATTTATCGGTTCTCCTAGTATAGCTATGTTAATTTCATTATTGTTTGCAATGTGGTTGATGGGTTATCACCGTA
AGATTGCTAGTAAAGATATTATGGCTACTTTAGAAGATGCGGTGAAATCAATTGCAATGCTCCTTCTGGTAATTGGTGGT
GGAGGTGCGTTTAAACAAGTTTTAATTGATGGTGGTGTTGGAGATGCGGTCAAAAATATCTTTGCATCATCTAGTATCTC
TCCTCTAATTTTAGGATGGTTGATAGCGGTAGTCCTTCGAGTGGCTTTAGGATCCGCAACGGTGGCATCTCTAACTGCTG
CCGGTTTAGTATTACCCTTAATGCAAGGGGCGGGAATTGATCCTGCATTAATGGTGTTATCAGTTGGAGCAGGAAGTTTA
GCTGCGAGTCACGTTAATGATGCAGGCTTCTGGATGTTCAAAGAATACTTTGATTTATCCGTTAAAGAAACCTTACTAAC
ATGGACAGTACTAGAAACAGTCATTTCGATTGTTGGATTAATTGGCGTGTTATTATTAAGTTTGATTGTTTAA

Upstream 100 bases:

>100_bases
AACATGCAAAAATAGCTGAAGAAGATTAAAGAGACGATCAACGGTCTTTTTAATTTAATAGCAAAAAGAAAGCGGTTACT
TAGATGGGGGCGTGTTATTT

Downstream 100 bases:

>100_bases
CTTAAAGGCATTAAAATAGCTATCTCACCACGTTTCCCGTAAACTGTAAGTGGGAGGTGGATTAAATGAAAAGAGCTCTT
TCAATTATTGCAATAGTATT

Product: H+/gluconate symporter related permease

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MEIMVLVIGIILLLTLIIKFKMNTYVSLIITAVVVGLGLGMPASQIATTIQNGIGSQLGELALVFGFGAMLGRLVADAGG
AYRIANTLIDKFGRRFLQIAVVVASFIIGIALFFEVGIVLLVPIVFAIAIEAGVPVLSLGIPMAAALSVTHGFLPPHPAP
TAIAAVLNANAGKVLLYGVIIAIPTVYIAGVLFSKIAKKYLPEAFKRNGNLKALGPQKQFKLEETPGFGISVLTALFPVI
LMAITTIYELIVHDGVTPKNPNAVDNFFGFIGSPSIAMLISLLFAMWLMGYHRKIASKDIMATLEDAVKSIAMLLLVIGG
GGAFKQVLIDGGVGDAVKNIFASSSISPLILGWLIAVVLRVALGSATVASLTAAGLVLPLMQGAGIDPALMVLSVGAGSL
AASHVNDAGFWMFKEYFDLSVKETLLTWTVLETVISIVGLIGVLLLSLIV

Sequences:

>Translated_450_residues
MEIMVLVIGIILLLTLIIKFKMNTYVSLIITAVVVGLGLGMPASQIATTIQNGIGSQLGELALVFGFGAMLGRLVADAGG
AYRIANTLIDKFGRRFLQIAVVVASFIIGIALFFEVGIVLLVPIVFAIAIEAGVPVLSLGIPMAAALSVTHGFLPPHPAP
TAIAAVLNANAGKVLLYGVIIAIPTVYIAGVLFSKIAKKYLPEAFKRNGNLKALGPQKQFKLEETPGFGISVLTALFPVI
LMAITTIYELIVHDGVTPKNPNAVDNFFGFIGSPSIAMLISLLFAMWLMGYHRKIASKDIMATLEDAVKSIAMLLLVIGG
GGAFKQVLIDGGVGDAVKNIFASSSISPLILGWLIAVVLRVALGSATVASLTAAGLVLPLMQGAGIDPALMVLSVGAGSL
AASHVNDAGFWMFKEYFDLSVKETLLTWTVLETVISIVGLIGVLLLSLIV
>Mature_450_residues
MEIMVLVIGIILLLTLIIKFKMNTYVSLIITAVVVGLGLGMPASQIATTIQNGIGSQLGELALVFGFGAMLGRLVADAGG
AYRIANTLIDKFGRRFLQIAVVVASFIIGIALFFEVGIVLLVPIVFAIAIEAGVPVLSLGIPMAAALSVTHGFLPPHPAP
TAIAAVLNANAGKVLLYGVIIAIPTVYIAGVLFSKIAKKYLPEAFKRNGNLKALGPQKQFKLEETPGFGISVLTALFPVI
LMAITTIYELIVHDGVTPKNPNAVDNFFGFIGSPSIAMLISLLFAMWLMGYHRKIASKDIMATLEDAVKSIAMLLLVIGG
GGAFKQVLIDGGVGDAVKNIFASSSISPLILGWLIAVVLRVALGSATVASLTAAGLVLPLMQGAGIDPALMVLSVGAGSL
AASHVNDAGFWMFKEYFDLSVKETLLTWTVLETVISIVGLIGVLLLSLIV

Specific function: Part Of The Gluconate Utilization System Gnt-I; High- Affinity Intake Of Gluconate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=453, Percent_Identity=50.7726269315673, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1790716, Length=444, Percent_Identity=49.7747747747748, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI2367370, Length=451, Percent_Identity=49.8891352549889, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1790776, Length=461, Percent_Identity=40.997830802603, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI1788707, Length=449, Percent_Identity=34.2984409799555, Blast_Score=236, Evalue=2e-63,
Organism=Escherichia coli, GI48994939, Length=445, Percent_Identity=35.2808988764045, Blast_Score=233, Evalue=1e-62,
Organism=Escherichia coli, GI1789097, Length=441, Percent_Identity=34.0136054421769, Blast_Score=218, Evalue=7e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 47090; Mature: 47090

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIMVLVIGIILLLTLIIKFKMNTYVSLIITAVVVGLGLGMPASQIATTIQNGIGSQLGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LALVFGFGAMLGRLVADAGGAYRIANTLIDKFGRRFLQIAVVVASFIIGIALFFEVGIVL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPIVFAIAIEAGVPVLSLGIPMAAALSVTHGFLPPHPAPTAIAAVLNANAGKVLLYGVI
HHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHH
IAIPTVYIAGVLFSKIAKKYLPEAFKRNGNLKALGPQKQFKLEETPGFGISVLTALFPVI
HHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHH
LMAITTIYELIVHDGVTPKNPNAVDNFFGFIGSPSIAMLISLLFAMWLMGYHRKIASKDI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MATLEDAVKSIAMLLLVIGGGGAFKQVLIDGGVGDAVKNIFASSSISPLILGWLIAVVLR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
VALGSATVASLTAAGLVLPLMQGAGIDPALMVLSVGAGSLAASHVNDAGFWMFKEYFDLS
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCH
VKETLLTWTVLETVISIVGLIGVLLLSLIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEIMVLVIGIILLLTLIIKFKMNTYVSLIITAVVVGLGLGMPASQIATTIQNGIGSQLGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LALVFGFGAMLGRLVADAGGAYRIANTLIDKFGRRFLQIAVVVASFIIGIALFFEVGIVL
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVPIVFAIAIEAGVPVLSLGIPMAAALSVTHGFLPPHPAPTAIAAVLNANAGKVLLYGVI
HHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCHHHHHHHH
IAIPTVYIAGVLFSKIAKKYLPEAFKRNGNLKALGPQKQFKLEETPGFGISVLTALFPVI
HHHHHHHHHHHHHHHHHHHHCHHHHHCCCCEEEECCHHHCCCCCCCCCHHHHHHHHHHHH
LMAITTIYELIVHDGVTPKNPNAVDNFFGFIGSPSIAMLISLLFAMWLMGYHRKIASKDI
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MATLEDAVKSIAMLLLVIGGGGAFKQVLIDGGVGDAVKNIFASSSISPLILGWLIAVVLR
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHH
VALGSATVASLTAAGLVLPLMQGAGIDPALMVLSVGAGSLAASHVNDAGFWMFKEYFDLS
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHCCCHHHHHHHHHHCCH
VKETLLTWTVLETVISIVGLIGVLLLSLIV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9734814; 9384377 [H]