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The map label for this gene is ytwF [H]

Identifier: 116493090

GI number: 116493090

Start: 1330956

End: 1331252

Strand: Reverse

Name: ytwF [H]

Synonym: PEPE_1344

Alternate gene names: 116493090

Gene position: 1331252-1330956 (Counterclockwise)

Preceding gene: 116493091

Following gene: 116493089

Centisome position: 72.65

GC content: 37.04

Gene sequence:

>297_bases
ATGATTAATTCGATTGAAAATGATGATTTTTACCAAAAGATGCAAAATGAAAAATTAACGGTTTTAGATGTACGTGAGAA
AGTAGAATTTATCGATGGTCATGTACCGATGGCACAAAATTGGCCCTTGAGTGAATTAGCCGAAAAATATAGCAAATTAG
ATACTAGCACATCTTACTATGTGATTTGTCGTTCAGGGGCACGCTCAGCAAATGCATGTGCTTTCTTGCAAGATAAGGGA
TTGAACGTTACTAATGTAACTGGAGGGATGCTAGCTTGGAAGGGCGAAGTTATTTAG

Upstream 100 bases:

>100_bases
AAGGTTAATATTCGTGATTACTGAAACCTATAAAAAATGAGCCGTAAAATATGATAAAATGCTCCGATAGGTATTTTTAT
AGATATAGGAGAGTAAACTA

Downstream 100 bases:

>100_bases
TAGTGGGGGATAAAATTAACAAAATGTTAAATGGAAAGCAAAAGAGTCATGTAGTGTGAAAACTACATGACTCTTTTTAG
GTTGATTGGCCATACTGGGC

Product: rhodanese-related sulfurtransferase

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 98; Mature: 98

Protein sequence:

>98_residues
MINSIENDDFYQKMQNEKLTVLDVREKVEFIDGHVPMAQNWPLSELAEKYSKLDTSTSYYVICRSGARSANACAFLQDKG
LNVTNVTGGMLAWKGEVI

Sequences:

>Translated_98_residues
MINSIENDDFYQKMQNEKLTVLDVREKVEFIDGHVPMAQNWPLSELAEKYSKLDTSTSYYVICRSGARSANACAFLQDKG
LNVTNVTGGMLAWKGEVI
>Mature_98_residues
MINSIENDDFYQKMQNEKLTVLDVREKVEFIDGHVPMAQNWPLSELAEKYSKLDTSTSYYVICRSGARSANACAFLQDKG
LNVTNVTGGMLAWKGEVI

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11038; Mature: 11038

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSIENDDFYQKMQNEKLTVLDVREKVEFIDGHVPMAQNWPLSELAEKYSKLDTSTSYY
CCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
VICRSGARSANACAFLQDKGLNVTNVTGGMLAWKGEVI
EEECCCCCCCCEEEEEECCCCEEEECCCCEEEECCCCC
>Mature Secondary Structure
MINSIENDDFYQKMQNEKLTVLDVREKVEFIDGHVPMAQNWPLSELAEKYSKLDTSTSYY
CCCCCCCCHHHHHHCCCCEEEEEHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCEE
VICRSGARSANACAFLQDKGLNVTNVTGGMLAWKGEVI
EEECCCCCCCCEEEEEECCCCEEEECCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]