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The map label for this gene is ddl

Identifier: 116493050

GI number: 116493050

Start: 1293691

End: 1294746

Strand: Reverse

Name: ddl

Synonym: PEPE_1302

Alternate gene names: 116493050

Gene position: 1294746-1293691 (Counterclockwise)

Preceding gene: 116493051

Following gene: 116493049

Centisome position: 70.66

GC content: 37.59

Gene sequence:

>1056_bases
ATGAAAATTGCTGTACTTGCCGGTGGGAAAAGCACGGAAAGAAACGTGTCGCTTTCATCTGGTTCAAAGATCACCAATGC
TTTGCGATCAAAAGGTTACGATGCAACGATGATTGATTTATTCTTGGGATATGAACTTGAGGATGGTCAAAGTTATGAAG
ACGTATTTAAGTCTAGTAATACCAGTACTGATTATGAAATCAGTGACGCAGTTTTAACAGAAGAAGATATTGAAGAACTT
CGTACAGACGGTACGGTTGGATTGTTTGGGAAAAATGTTTTACCAATCCTCCAAGCTGCTGACTTTGTTTTTCTAGCGTT
ACATGGTGGTGATGGTGAGAACGGTAAAGTTCAAGCTGTTTTGGATCTTAATCATATTAAATATACAGGTAGTGGTTCAC
TAGCTTCAGGAATTGCCATGGATAAAGCAATTTCAAAAGAGATAATGTTGTATAACAACATTAAAACAGCCCAATTTGCT
GTATTGCATGCAAAAGATGGTATTCATCCTCAATTAGCCTTTGACTATCCAATGGTTGTTAAGCCTAATAGTGGTGGTTC
AAGTATTGGTACTAGAATTGTACATGATGAAGCCGAACTAGCTGAATCATTAAAGGATGCCTATCGTTTTGATGATGAAA
TTATTGTCGAAGAATTTATCACTGGACGTGAATTTTCACTAGGGGTGGTAAATGGTCAAGCAATGCCAGCAATTGAAATT
GTGGTAAATGATGGTTGGTACGATTATGAACATAAATTTCAAACGGGTTCAACGACTAAATTCGTTACTCCTCCAGAAAT
CGAAGATGATGTTCATGATGAAATGAAAAGGGTAGCTGTTCAAACAATGGATGCCTTGGGCATGACTAACTATGGACGCG
TTGATTTTCTAACTAACGACACAGGCGTATATGTAATCGAAGCCAATAACCTTCCAGGAATGACACCTCTATCGTTATTA
CCACAAGAAGCAGAAGCAGCTGGTATTAGTTATGAGGACCTTTGTGAAAGTATCGTATTAGGCAAGCAAAAGCTTTACGA
TGAATTAAAAAAATAA

Upstream 100 bases:

>100_bases
ATTGAACTGATTTTATGATATACTGAAGGTTCAATAAACGTTTGATAATTGGCGGAGCAAACTCAAGGTTTGTAATTATC
TTACATACGAGGTGGAAAAA

Downstream 100 bases:

>100_bases
ATTTAAAATCCCTCTTTAGAGTAATTAAACCCTAAGGAGGGATTTTTTATGAAATTAAAATGTACGTGGTCAACTAAGAA
TGCAATTTTAGAAAACTATC

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKIAVLAGGKSTERNVSLSSGSKITNALRSKGYDATMIDLFLGYELEDGQSYEDVFKSSNTSTDYEISDAVLTEEDIEEL
RTDGTVGLFGKNVLPILQAADFVFLALHGGDGENGKVQAVLDLNHIKYTGSGSLASGIAMDKAISKEIMLYNNIKTAQFA
VLHAKDGIHPQLAFDYPMVVKPNSGGSSIGTRIVHDEAELAESLKDAYRFDDEIIVEEFITGREFSLGVVNGQAMPAIEI
VVNDGWYDYEHKFQTGSTTKFVTPPEIEDDVHDEMKRVAVQTMDALGMTNYGRVDFLTNDTGVYVIEANNLPGMTPLSLL
PQEAEAAGISYEDLCESIVLGKQKLYDELKK

Sequences:

>Translated_351_residues
MKIAVLAGGKSTERNVSLSSGSKITNALRSKGYDATMIDLFLGYELEDGQSYEDVFKSSNTSTDYEISDAVLTEEDIEEL
RTDGTVGLFGKNVLPILQAADFVFLALHGGDGENGKVQAVLDLNHIKYTGSGSLASGIAMDKAISKEIMLYNNIKTAQFA
VLHAKDGIHPQLAFDYPMVVKPNSGGSSIGTRIVHDEAELAESLKDAYRFDDEIIVEEFITGREFSLGVVNGQAMPAIEI
VVNDGWYDYEHKFQTGSTTKFVTPPEIEDDVHDEMKRVAVQTMDALGMTNYGRVDFLTNDTGVYVIEANNLPGMTPLSLL
PQEAEAAGISYEDLCESIVLGKQKLYDELKK
>Mature_351_residues
MKIAVLAGGKSTERNVSLSSGSKITNALRSKGYDATMIDLFLGYELEDGQSYEDVFKSSNTSTDYEISDAVLTEEDIEEL
RTDGTVGLFGKNVLPILQAADFVFLALHGGDGENGKVQAVLDLNHIKYTGSGSLASGIAMDKAISKEIMLYNNIKTAQFA
VLHAKDGIHPQLAFDYPMVVKPNSGGSSIGTRIVHDEAELAESLKDAYRFDDEIIVEEFITGREFSLGVVNGQAMPAIEI
VVNDGWYDYEHKFQTGSTTKFVTPPEIEDDVHDEMKRVAVQTMDALGMTNYGRVDFLTNDTGVYVIEANNLPGMTPLSLL
PQEAEAAGISYEDLCESIVLGKQKLYDELKK

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=347, Percent_Identity=31.9884726224784, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI1786579, Length=350, Percent_Identity=28.8571428571429, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PEDPA (Q03EM9)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804785.1
- ProteinModelPortal:   Q03EM9
- SMR:   Q03EM9
- STRING:   Q03EM9
- GeneID:   4417557
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1302
- NMPDR:   fig|278197.10.peg.1143
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   YTARYTA
- PhylomeDB:   Q03EM9
- ProtClustDB:   CLSK770750
- BioCyc:   PPEN278197:PEPE_1302-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 38351; Mature: 38351

Theoretical pI: Translated: 4.24; Mature: 4.24

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAVLAGGKSTERNVSLSSGSKITNALRSKGYDATMIDLFLGYELEDGQSYEDVFKSSN
CEEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCCEEEEEEECEEECCCCCHHHHHHCCC
TSTDYEISDAVLTEEDIEELRTDGTVGLFGKNVLPILQAADFVFLALHGGDGENGKVQAV
CCCCEEECCCEECHHHHHHHHCCCCEEECCCCHHHHHHHCCEEEEEEECCCCCCCEEEEE
LDLNHIKYTGSGSLASGIAMDKAISKEIMLYNNIKTAQFAVLHAKDGIHPQLAFDYPMVV
EEECEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCCCEEEECCCEEE
KPNSGGSSIGTRIVHDEAELAESLKDAYRFDDEIIVEEFITGREFSLGVVNGQAMPAIEI
ECCCCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCEEEE
VVNDGWYDYEHKFQTGSTTKFVTPPEIEDDVHDEMKRVAVQTMDALGMTNYGRVDFLTND
EECCCEECCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGVYVIEANNLPGMTPLSLLPQEAEAAGISYEDLCESIVLGKQKLYDELKK
CCEEEEECCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure
MKIAVLAGGKSTERNVSLSSGSKITNALRSKGYDATMIDLFLGYELEDGQSYEDVFKSSN
CEEEEEECCCCCCCEEECCCCHHHHHHHHHCCCCCEEEEEEECEEECCCCCHHHHHHCCC
TSTDYEISDAVLTEEDIEELRTDGTVGLFGKNVLPILQAADFVFLALHGGDGENGKVQAV
CCCCEEECCCEECHHHHHHHHCCCCEEECCCCHHHHHHHCCEEEEEEECCCCCCCEEEEE
LDLNHIKYTGSGSLASGIAMDKAISKEIMLYNNIKTAQFAVLHAKDGIHPQLAFDYPMVV
EEECEEEEECCCCHHHHHHHHHHHHCCEEEEECCCCEEEEEEEECCCCCCEEEECCCEEE
KPNSGGSSIGTRIVHDEAELAESLKDAYRFDDEIIVEEFITGREFSLGVVNGQAMPAIEI
ECCCCCCCCCCEEEECHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCEEEE
VVNDGWYDYEHKFQTGSTTKFVTPPEIEDDVHDEMKRVAVQTMDALGMTNYGRVDFLTND
EECCCEECCCEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGVYVIEANNLPGMTPLSLLPQEAEAAGISYEDLCESIVLGKQKLYDELKK
CCEEEEECCCCCCCCHHHHCCCCHHHCCCCHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA