Click here to switch to the map view.

The map label for this gene is recA [H]

Identifier: 116493024

GI number: 116493024

Start: 1266418

End: 1267476

Strand: Reverse

Name: recA [H]

Synonym: PEPE_1276

Alternate gene names: 116493024

Gene position: 1267476-1266418 (Counterclockwise)

Preceding gene: 116493025

Following gene: 116493023

Centisome position: 69.17

GC content: 40.6

Gene sequence:

>1059_bases
TTGGCAGACGAAAGACAAGAAGCTTTAAATAAAGCACTTAAAAAAATTGAAAAAAACTTTGGTAAAGGATCTATTATGCG
AATGGGGGATGCGGTTGATACGCAAATTTCAACGATTCCCAGTGGTTCTTTAGCATTAGACGATGCACTAGGAGTTGGCG
GTTTTCCTAAGGGAAGAATCGTCGAAATTTATGGGCCTGAAAGTTCTGGTAAGACAACTGTAGCTTTACATGCAGTTGCT
GAAGTGCAAAAACGTGGGGGAACTGCTGCATATATTGATGCTGAAAACGCGATGGATCCTGCATATGCAACTAATTTGGG
CGTTGATATCGATTCACTCTTACTTTCACAACCAGACACTGGTGAAGAAGGATTAGCTATTGCCGATGCTTTGGTATCAA
GTGGTGCTATTGACTTGGTCGTTATTGATTCCGTTGCAGCCTTAGTTCCACGTGCTGAAATTGATGGAGAGATGGGGGAT
GCTCATGTTGGCCTCCAAGCTCGGATGATGTCACAAGCACTTCGTAAATTATCTGGTTCGATTAACAAAACTAAGACGAT
TGCGTTATTCATTAACCAAATACGTGAAAAAGTCGGAATTATGTTTGGTAATCCAGAAACAACTCCTGGTGGACGGGCTT
TAAAATTCTATGCAACAATCCGTTTAGAAGTACGCCGTGCAGAACAAATTAAAGATGGTGCCGACGTTATTGGTAACCGT
GTTCGTATTAAAGTGGTAAAAAATAAGGTTGCTCCTCCATTCAAAAAGGCTGAAGTCGATATTATGTATGGTCATGGAAT
TTCGCAAACTGGTGAGATGATTGATATGGCTGTTGAAAAAGATTTGGTTGGTAAGAGTGGTTCATGGTATTCATACGGAG
ACGAACGCATTGGACAGGGTCGTGAGAATGCTAAGAAGTACATTGATGAACATCCAGACGTTCGTGCAGAAATTCTTGGT
AAAGTTCGTGAAGCGTATGGTATGGTAGCTGATCCTACGAGTGAAGTTAAAAAAGAAAGTTCCAAAAAATCTTCAACTGA
TGATTCAGATTTAGCTTAA

Upstream 100 bases:

>100_bases
TTATTAAATAAAACATTCGAACTTTTGTTCGCTTTTTTGTTGATTTTTATAAAATAACTATGTATCATACTAGTATAGAG
AAACCCTAGGAGGTTATTAA

Downstream 100 bases:

>100_bases
ATACTACTAAAAGATTCAAGAAACTTTTCTTGAATCTTTTTTGATAGGTTTCAAACTAAATGATTCATTGACACTCTTAT
ACCTAAATATTAAAATGATA

Product: RecA protein

Products: NA

Alternate protein names: Recombinase A [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MADERQEALNKALKKIEKNFGKGSIMRMGDAVDTQISTIPSGSLALDDALGVGGFPKGRIVEIYGPESSGKTTVALHAVA
EVQKRGGTAAYIDAENAMDPAYATNLGVDIDSLLLSQPDTGEEGLAIADALVSSGAIDLVVIDSVAALVPRAEIDGEMGD
AHVGLQARMMSQALRKLSGSINKTKTIALFINQIREKVGIMFGNPETTPGGRALKFYATIRLEVRRAEQIKDGADVIGNR
VRIKVVKNKVAPPFKKAEVDIMYGHGISQTGEMIDMAVEKDLVGKSGSWYSYGDERIGQGRENAKKYIDEHPDVRAEILG
KVREAYGMVADPTSEVKKESSKKSSTDDSDLA

Sequences:

>Translated_352_residues
MADERQEALNKALKKIEKNFGKGSIMRMGDAVDTQISTIPSGSLALDDALGVGGFPKGRIVEIYGPESSGKTTVALHAVA
EVQKRGGTAAYIDAENAMDPAYATNLGVDIDSLLLSQPDTGEEGLAIADALVSSGAIDLVVIDSVAALVPRAEIDGEMGD
AHVGLQARMMSQALRKLSGSINKTKTIALFINQIREKVGIMFGNPETTPGGRALKFYATIRLEVRRAEQIKDGADVIGNR
VRIKVVKNKVAPPFKKAEVDIMYGHGISQTGEMIDMAVEKDLVGKSGSWYSYGDERIGQGRENAKKYIDEHPDVRAEILG
KVREAYGMVADPTSEVKKESSKKSSTDDSDLA
>Mature_351_residues
ADERQEALNKALKKIEKNFGKGSIMRMGDAVDTQISTIPSGSLALDDALGVGGFPKGRIVEIYGPESSGKTTVALHAVAE
VQKRGGTAAYIDAENAMDPAYATNLGVDIDSLLLSQPDTGEEGLAIADALVSSGAIDLVVIDSVAALVPRAEIDGEMGDA
HVGLQARMMSQALRKLSGSINKTKTIALFINQIREKVGIMFGNPETTPGGRALKFYATIRLEVRRAEQIKDGADVIGNRV
RIKVVKNKVAPPFKKAEVDIMYGHGISQTGEMIDMAVEKDLVGKSGSWYSYGDERIGQGRENAKKYIDEHPDVRAEILGK
VREAYGMVADPTSEVKKESSKKSSTDDSDLA

Specific function: Can catalyze the hydrolysis of ATP in the presence of single-stranded DNA, the ATP-dependent uptake of single-stranded DNA by duplex DNA, and the ATP-dependent hybridization of homologous single-stranded DNAs. It interacts with lexA causing its activation

COG id: COG0468

COG function: function code L; RecA/RadA recombinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family [H]

Homologues:

Organism=Escherichia coli, GI1789051, Length=348, Percent_Identity=56.3218390804598, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: 1548 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800-1200 (L-broth) 40,000-60,000 (L-broth + Nalidixate) 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013765
- InterPro:   IPR020584
- InterPro:   IPR020588
- InterPro:   IPR020587 [H]

Pfam domain/function: PF00154 RecA [H]

EC number: NA

Molecular weight: Translated: 37698; Mature: 37567

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00321 RECA_1 ; PS50162 RECA_2 ; PS50163 RECA_3 ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADERQEALNKALKKIEKNFGKGSIMRMGDAVDTQISTIPSGSLALDDALGVGGFPKGRI
CCCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCCCEEEHHHCCCCCCCCCCE
VEIYGPESSGKTTVALHAVAEVQKRGGTAAYIDAENAMDPAYATNLGVDIDSLLLSQPDT
EEEECCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCHHHHHHCCCCC
GEEGLAIADALVSSGAIDLVVIDSVAALVPRAEIDGEMGDAHVGLQARMMSQALRKLSGS
CCCCHHHHHHHHCCCCEEEEEHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
INKTKTIALFINQIREKVGIMFGNPETTPGGRALKFYATIRLEVRRAEQIKDGADVIGNR
CCCHHHHHHHHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEEEHHHHHHHCCCHHHCCCC
VRIKVVKNKVAPPFKKAEVDIMYGHGISQTGEMIDMAVEKDLVGKSGSWYSYGDERIGQG
EEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC
RENAKKYIDEHPDVRAEILGKVREAYGMVADPTSEVKKESSKKSSTDDSDLA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
ADERQEALNKALKKIEKNFGKGSIMRMGDAVDTQISTIPSGSLALDDALGVGGFPKGRI
CCHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCCCCEEEHHHCCCCCCCCCCE
VEIYGPESSGKTTVALHAVAEVQKRGGTAAYIDAENAMDPAYATNLGVDIDSLLLSQPDT
EEEECCCCCCCEEEEHHHHHHHHHCCCCEEEEECCCCCCCHHHHCCCCCHHHHHHCCCCC
GEEGLAIADALVSSGAIDLVVIDSVAALVPRAEIDGEMGDAHVGLQARMMSQALRKLSGS
CCCCHHHHHHHHCCCCEEEEEHHHHHHHHCHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
INKTKTIALFINQIREKVGIMFGNPETTPGGRALKFYATIRLEVRRAEQIKDGADVIGNR
CCCHHHHHHHHHHHHHHHCEEECCCCCCCCCCEEEEEEEEEEEHHHHHHHCCCHHHCCCC
VRIKVVKNKVAPPFKKAEVDIMYGHGISQTGEMIDMAVEKDLVGKSGSWYSYGDERIGQG
EEEEEEECCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHCCC
RENAKKYIDEHPDVRAEILGKVREAYGMVADPTSEVKKESSKKSSTDDSDLA
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]