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The map label for this gene is queA

Identifier: 116493018

GI number: 116493018

Start: 1257303

End: 1258337

Strand: Reverse

Name: queA

Synonym: PEPE_1270

Alternate gene names: 116493018

Gene position: 1258337-1257303 (Counterclockwise)

Preceding gene: 116493019

Following gene: 116493017

Centisome position: 68.67

GC content: 37.58

Gene sequence:

>1035_bases
ATGACACTAACTACTGAAGATTTTAATTATGACTTACCACATGAATTAATTGCCCAAACTCCAATTAAAGAACGCGATGA
ATCTCGTTTACTTGTGTTAGACCACGAAACAGGAGCAATGGAAGATAAACATTTTTACGATATTATCGACCAATTAAATC
CTGGTGATGCAGTCGTTATGAATAATTCACGAGTAATGCCGGCTCGTATTTACGGAATTAAGGACAAAACGGGTGGTCAC
TTTGAAGTTCTACTATTACACAATATTGAAGGCGACCGCTGGGAAACGCTCGTTAAACCTGCTAAACGAGCAAAAACTGG
AACCAAAATTATATTTGGTGATGGTGCCTTGACAGCGACAGTTACCAAAGAATTAGAACATGGTGGACGTGAGATTGTCT
TTGATTATGATGGAATTTTCATGGAAGTTTTGGAAAAGCTAGGAGAAATGCCGTTACCTCCATACATCAAAGAAAAATTA
GATGATCCAGAACGTTATCAGACCGTTTATTCTAAAGAACCAGGTTCAGCAGCAGCTCCAACGGCTGGATTACACTGGAC
TAAAGAACTACTAGAAAAGGTTGAACAAAAGGGCATCAAATTAGTTTATTTGACATTGCATGTTGGACTAGGAACTTTCC
GACCTGTGGATGAAGATAATATTGAAGATCACAAGATGCACAGTGAGTTTTATCAACTTTCTGAAGAATCAGCGGCAGCC
TTGAATGAAGTGAAGAAGCAGGGTGGAAGAATTGTGGCTACGGGAACAACTTCAATTCGGACGCTTGAAACAATTGGCTC
TAAGTTTAATGGAGAAATTAAAGCTGATAGTGGTTGGACAGATATTTTTATTAAACCAGGTTATGAATGGAAAGTGGTTG
ACGCTTTTATAACTAATTTCCATTTACCAAAATCAACTTTGGTAATGCTAGTAGCGGCCTTTACGGGAAGAGAAAATATT
TTAAATGCTTACCAACATGCGATTGAAGAAAAATATCGTTTCTTTAGCTTTGGTGACGCAATGTTTATTAAATAA

Upstream 100 bases:

>100_bases
GGATTCCTGAAAAGGACGCCTCGAGGCCGGATGGTTACAGAACGTGCCTACGAACATCTAGGCTTAATTGATCAATATAT
GAATAAATGAGGAATTGAAA

Downstream 100 bases:

>100_bases
AGAACTATGAATGGAGAAATACATTGGAACCTGCAATTAAATACCGATTAATTAAAACCGATAAGCATACAGGAGCACGT
CTAGGTGAAATAATCACCCC

Product: S-adenosylmethionine:tRNA-ribosyltransferase- isomerase (queuine synthetase)

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 344; Mature: 343

Protein sequence:

>344_residues
MTLTTEDFNYDLPHELIAQTPIKERDESRLLVLDHETGAMEDKHFYDIIDQLNPGDAVVMNNSRVMPARIYGIKDKTGGH
FEVLLLHNIEGDRWETLVKPAKRAKTGTKIIFGDGALTATVTKELEHGGREIVFDYDGIFMEVLEKLGEMPLPPYIKEKL
DDPERYQTVYSKEPGSAAAPTAGLHWTKELLEKVEQKGIKLVYLTLHVGLGTFRPVDEDNIEDHKMHSEFYQLSEESAAA
LNEVKKQGGRIVATGTTSIRTLETIGSKFNGEIKADSGWTDIFIKPGYEWKVVDAFITNFHLPKSTLVMLVAAFTGRENI
LNAYQHAIEEKYRFFSFGDAMFIK

Sequences:

>Translated_344_residues
MTLTTEDFNYDLPHELIAQTPIKERDESRLLVLDHETGAMEDKHFYDIIDQLNPGDAVVMNNSRVMPARIYGIKDKTGGH
FEVLLLHNIEGDRWETLVKPAKRAKTGTKIIFGDGALTATVTKELEHGGREIVFDYDGIFMEVLEKLGEMPLPPYIKEKL
DDPERYQTVYSKEPGSAAAPTAGLHWTKELLEKVEQKGIKLVYLTLHVGLGTFRPVDEDNIEDHKMHSEFYQLSEESAAA
LNEVKKQGGRIVATGTTSIRTLETIGSKFNGEIKADSGWTDIFIKPGYEWKVVDAFITNFHLPKSTLVMLVAAFTGRENI
LNAYQHAIEEKYRFFSFGDAMFIK
>Mature_343_residues
TLTTEDFNYDLPHELIAQTPIKERDESRLLVLDHETGAMEDKHFYDIIDQLNPGDAVVMNNSRVMPARIYGIKDKTGGHF
EVLLLHNIEGDRWETLVKPAKRAKTGTKIIFGDGALTATVTKELEHGGREIVFDYDGIFMEVLEKLGEMPLPPYIKEKLD
DPERYQTVYSKEPGSAAAPTAGLHWTKELLEKVEQKGIKLVYLTLHVGLGTFRPVDEDNIEDHKMHSEFYQLSEESAAAL
NEVKKQGGRIVATGTTSIRTLETIGSKFNGEIKADSGWTDIFIKPGYEWKVVDAFITNFHLPKSTLVMLVAAFTGRENIL
NAYQHAIEEKYRFFSFGDAMFIK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=342, Percent_Identity=47.953216374269, Blast_Score=326, Evalue=1e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PEDPA (Q03ER1)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804753.1
- ProteinModelPortal:   Q03ER1
- SMR:   Q03ER1
- STRING:   Q03ER1
- GeneID:   4417606
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1270
- NMPDR:   fig|278197.10.peg.1112
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   SEFYRLT
- PhylomeDB:   Q03ER1
- BioCyc:   PPEN278197:PEPE_1270-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38901; Mature: 38770

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTTEDFNYDLPHELIAQTPIKERDESRLLVLDHETGAMEDKHFYDIIDQLNPGDAVVM
CCCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEE
NNSRVMPARIYGIKDKTGGHFEVLLLHNIEGDRWETLVKPAKRAKTGTKIIFGDGALTAT
CCCEECEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCEEHH
VTKELEHGGREIVFDYDGIFMEVLEKLGEMPLPPYIKEKLDDPERYQTVYSKEPGSAAAP
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCC
TAGLHWTKELLEKVEQKGIKLVYLTLHVGLGTFRPVDEDNIEDHKMHSEFYQLSEESAAA
CCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNEVKKQGGRIVATGTTSIRTLETIGSKFNGEIKADSGWTDIFIKPGYEWKVVDAFITNF
HHHHHHCCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEEHHHHHHHC
HLPKSTLVMLVAAFTGRENILNAYQHAIEEKYRFFSFGDAMFIK
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEC
>Mature Secondary Structure 
TLTTEDFNYDLPHELIAQTPIKERDESRLLVLDHETGAMEDKHFYDIIDQLNPGDAVVM
CCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEE
NNSRVMPARIYGIKDKTGGHFEVLLLHNIEGDRWETLVKPAKRAKTGTKIIFGDGALTAT
CCCEECEEEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHCCCCCEEEEECCCEEHH
VTKELEHGGREIVFDYDGIFMEVLEKLGEMPLPPYIKEKLDDPERYQTVYSKEPGSAAAP
HHHHHHCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHCCCCCCCCCC
TAGLHWTKELLEKVEQKGIKLVYLTLHVGLGTFRPVDEDNIEDHKMHSEFYQLSEESAAA
CCCHHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LNEVKKQGGRIVATGTTSIRTLETIGSKFNGEIKADSGWTDIFIKPGYEWKVVDAFITNF
HHHHHHCCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCEEEEEECCCCEEEHHHHHHHC
HLPKSTLVMLVAAFTGRENILNAYQHAIEEKYRFFSFGDAMFIK
CCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA