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The map label for this gene is tgt

Identifier: 116493017

GI number: 116493017

Start: 1256137

End: 1257279

Strand: Reverse

Name: tgt

Synonym: PEPE_1269

Alternate gene names: 116493017

Gene position: 1257279-1256137 (Counterclockwise)

Preceding gene: 116493018

Following gene: 116493016

Centisome position: 68.61

GC content: 39.37

Gene sequence:

>1143_bases
TTGGAACCTGCAATTAAATACCGATTAATTAAAACCGATAAGCATACAGGAGCACGTCTAGGTGAAATAATCACCCCGCA
TGGTACTTTTCCAACACCGATTTTTATGCCCGTTGGAACTCAAGCAACCGTCAAAGCCATGTCACCTGAAGAACTAGAAG
ACTTAGGAGCAGACATTATTTTGTCGAATACCTATCATTTATGGGTGCGACCAGGTGAAGATATTGTTAAAGAAGGTGGT
GGTCTTCACCAATTTATGAACTGGAAAAAGGGAATCTTAACTGATTCAGGTGGATTTCAGGTATTTTCTTTAGCGAAATT
ACGGGATATCACTGAAGAAGGGGTGCACTTTAAAAACGAGCTTAACGGAGCAAACATGTTCCTTTCACCGGAAAAGGCTA
TTCAAATTGAAAATGATCTGGGTCCAGATATCATGATGAGTTTCGATGAATGCCCGCCTTATTTTGAGAGTTATGACTAT
GTTAAACATTCGGTCGAAAGAACTAGCCGTTGGGCCGAACGTGGTTTGAAAGCTCATCGTAACCCAGAAACCCAAGGCCT
TTTTGGGATTATTCAAGGTGCCGGTTTCGAAGATTTAAGAAGACAAAGTGCTAAAGATTTAGTTAGTATGGATTTCCCTG
GATATTCAATCGGAGGACTTTCAGTAGGAGAGTCTAAAGAAGAAATGAATCGGGTGTTGGATTTCACAACGCAATTGATT
CCCGAAAATAAGCCCCGTTATTTAATGGGAGTAGGTTCGCCAGATGCTTTGATTGATGGTGTTCTTCGTGGGGTAGATAT
GTTTGATTGTGTATTACCAACCCGCATTGCCCGAAATGGTACCTGTATGACGAGTCATGGTCGTTTAGTAGTTAAAAATG
CTAAATACGCGCGTGATTTCACACCAATTGATGATAATTGTCAATGCTATACTTGTCGCAACTACACTCGGGCCTATATT
CGCCATCTAATTAAGACCGACGAAACTTTTGGTCTTCGACTAACCAGTATTCATAATGTCTATTTCTTAGTTCATTTGAT
GAAAGATGTGCGTCAAGCTATTATGGATGATAATTTACTTGAGTTTAGACAAAACTTCTTTGAAGAATACGGATATAATA
AGGAAAATAGTAAGAATTTCTAA

Upstream 100 bases:

>100_bases
TTTTAAATGCTTACCAACATGCGATTGAAGAAAAATATCGTTTCTTTAGCTTTGGTGACGCAATGTTTATTAAATAAAGA
ACTATGAATGGAGAAATACA

Downstream 100 bases:

>100_bases
GAGTACTGGTTTAGAAAACGTATTTTTGGTACAGTATACTTAGACGACTTTAGTGAGGTGAAAAAATGTTATTAGCAGCT
GCAAATGGTGGTTTCGGAAT

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MEPAIKYRLIKTDKHTGARLGEIITPHGTFPTPIFMPVGTQATVKAMSPEELEDLGADIILSNTYHLWVRPGEDIVKEGG
GLHQFMNWKKGILTDSGGFQVFSLAKLRDITEEGVHFKNELNGANMFLSPEKAIQIENDLGPDIMMSFDECPPYFESYDY
VKHSVERTSRWAERGLKAHRNPETQGLFGIIQGAGFEDLRRQSAKDLVSMDFPGYSIGGLSVGESKEEMNRVLDFTTQLI
PENKPRYLMGVGSPDALIDGVLRGVDMFDCVLPTRIARNGTCMTSHGRLVVKNAKYARDFTPIDDNCQCYTCRNYTRAYI
RHLIKTDETFGLRLTSIHNVYFLVHLMKDVRQAIMDDNLLEFRQNFFEEYGYNKENSKNF

Sequences:

>Translated_380_residues
MEPAIKYRLIKTDKHTGARLGEIITPHGTFPTPIFMPVGTQATVKAMSPEELEDLGADIILSNTYHLWVRPGEDIVKEGG
GLHQFMNWKKGILTDSGGFQVFSLAKLRDITEEGVHFKNELNGANMFLSPEKAIQIENDLGPDIMMSFDECPPYFESYDY
VKHSVERTSRWAERGLKAHRNPETQGLFGIIQGAGFEDLRRQSAKDLVSMDFPGYSIGGLSVGESKEEMNRVLDFTTQLI
PENKPRYLMGVGSPDALIDGVLRGVDMFDCVLPTRIARNGTCMTSHGRLVVKNAKYARDFTPIDDNCQCYTCRNYTRAYI
RHLIKTDETFGLRLTSIHNVYFLVHLMKDVRQAIMDDNLLEFRQNFFEEYGYNKENSKNF
>Mature_380_residues
MEPAIKYRLIKTDKHTGARLGEIITPHGTFPTPIFMPVGTQATVKAMSPEELEDLGADIILSNTYHLWVRPGEDIVKEGG
GLHQFMNWKKGILTDSGGFQVFSLAKLRDITEEGVHFKNELNGANMFLSPEKAIQIENDLGPDIMMSFDECPPYFESYDY
VKHSVERTSRWAERGLKAHRNPETQGLFGIIQGAGFEDLRRQSAKDLVSMDFPGYSIGGLSVGESKEEMNRVLDFTTQLI
PENKPRYLMGVGSPDALIDGVLRGVDMFDCVLPTRIARNGTCMTSHGRLVVKNAKYARDFTPIDDNCQCYTCRNYTRAYI
RHLIKTDETFGLRLTSIHNVYFLVHLMKDVRQAIMDDNLLEFRQNFFEEYGYNKENSKNF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=360, Percent_Identity=43.8888888888889, Blast_Score=338, Evalue=4e-93,
Organism=Escherichia coli, GI1786607, Length=368, Percent_Identity=52.1739130434783, Blast_Score=397, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17544680, Length=363, Percent_Identity=46.2809917355372, Blast_Score=318, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25146502, Length=206, Percent_Identity=31.0679611650485, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI19920510, Length=368, Percent_Identity=42.9347826086957, Blast_Score=325, Evalue=3e-89,
Organism=Drosophila melanogaster, GI21355311, Length=256, Percent_Identity=25.390625, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_PEDPA (Q03ER2)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804752.1
- ProteinModelPortal:   Q03ER2
- SMR:   Q03ER2
- STRING:   Q03ER2
- GeneID:   4417605
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1269
- NMPDR:   fig|278197.10.peg.1111
- eggNOG:   COG0343
- HOGENOM:   HBG629929
- OMA:   FMPVGTV
- PhylomeDB:   Q03ER2
- ProtClustDB:   PRK00112
- BioCyc:   PPEN278197:PEPE_1269-MONOMER
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 43207; Mature: 43207

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: NA

Important sites: ACT_SITE 95-95 BINDING 96-96

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEPAIKYRLIKTDKHTGARLGEIITPHGTFPTPIFMPVGTQATVKAMSPEELEDLGADII
CCCCEEEEEEECCCCCCHHHCCEECCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCCEE
LSNTYHLWVRPGEDIVKEGGGLHQFMNWKKGILTDSGGFQVFSLAKLRDITEEGVHFKNE
EECCEEEEEECCHHHHHCCCCHHHHHHHHCCCEECCCCCEEEHHHHHHHHHHHCCHHHHH
LNGANMFLSPEKAIQIENDLGPDIMMSFDECPPYFESYDYVKHSVERTSRWAERGLKAHR
CCCCCEEECCHHHEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPETQGLFGIIQGAGFEDLRRQSAKDLVSMDFPGYSIGGLSVGESKEEMNRVLDFTTQLI
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
PENKPRYLMGVGSPDALIDGVLRGVDMFDCVLPTRIARNGTCMTSHGRLVVKNAKYARDF
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHCCC
TPIDDNCQCYTCRNYTRAYIRHLIKTDETFGLRLTSIHNVYFLVHLMKDVRQAIMDDNLL
CCCCCCCEEEEECHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHH
EFRQNFFEEYGYNKENSKNF
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEPAIKYRLIKTDKHTGARLGEIITPHGTFPTPIFMPVGTQATVKAMSPEELEDLGADII
CCCCEEEEEEECCCCCCHHHCCEECCCCCCCCCEEEECCCCCEEECCCHHHHHHCCCCEE
LSNTYHLWVRPGEDIVKEGGGLHQFMNWKKGILTDSGGFQVFSLAKLRDITEEGVHFKNE
EECCEEEEEECCHHHHHCCCCHHHHHHHHCCCEECCCCCEEEHHHHHHHHHHHCCHHHHH
LNGANMFLSPEKAIQIENDLGPDIMMSFDECPPYFESYDYVKHSVERTSRWAERGLKAHR
CCCCCEEECCHHHEEECCCCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
NPETQGLFGIIQGAGFEDLRRQSAKDLVSMDFPGYSIGGLSVGESKEEMNRVLDFTTQLI
CCCCCCHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHC
PENKPRYLMGVGSPDALIDGVLRGVDMFDCVLPTRIARNGTCMTSHGRLVVKNAKYARDF
CCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEECCHHHCCC
TPIDDNCQCYTCRNYTRAYIRHLIKTDETFGLRLTSIHNVYFLVHLMKDVRQAIMDDNLL
CCCCCCCEEEEECHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCHHH
EFRQNFFEEYGYNKENSKNF
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA