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The map label for this gene is purA

Identifier: 116492995

GI number: 116492995

Start: 1232902

End: 1234197

Strand: Direct

Name: purA

Synonym: PEPE_1247

Alternate gene names: 116492995

Gene position: 1232902-1234197 (Clockwise)

Preceding gene: 116492973

Following gene: 116492997

Centisome position: 67.28

GC content: 37.11

Gene sequence:

>1296_bases
ATGACAGCTACAGTAGTAATAGGAAGCCAATGGGGCGATGAAGGAAAAGGAAAGATTATTGATTTTCTTGGACAAAGTGC
TGATGTTACAGTGCGTTATTCAGGTGGAGATAATGCTGGTCACTCACTTGTGGTTAACGACCAAAAATTAGCTCTTCGTT
TAATCCCTTCCGGAATTCTTGCTCCTAATTCAATTTGTATCATTGGTAATGGAACAGTGATTAATCCTGCTACCCTACTT
GATGAAATTAAAGAATTAAATGGAGTAGGTGTTAACACTGATCATTTAAAAATTTCTGATCGTGCTCATATTGTTTTCCC
ATATCACATCTTACAAGATCAACAACAAGAACGTGATCGTTCTAAAAATGGCGAAAAAATTGGAACAACTAATAAAGGTA
TCGGCCCAGCATACATGGACAAGATGCAACGTATTGGTATCCGAGCTATTGATCTATTAGACAACGAAACTCTTGAAGAA
AAAATTGCTTTTAACCTTGAACAAAAGAAACGCATTCTGGACGAAGATCTTTGGAATCAACTTCCAAGCTTAAAGGAATT
GACCGCTCAGTACATTGAATATGGAGAAATTCTTAAGGACTATATTACTGATACAAGCTACCTAATTCACACTAACTTAA
ATGATAATAAACGCGTCTTATTTGAAGGAGCTCAAGGTACTATGCTTGATATTGACCATGGTACTTATCCTTTTGTTACT
TCCTCTAATCCAACTGCCGGTGGAGCTGCTACGGGTGCAGGAATTGGTGTAACTAAGATTAAACACGTCATTGGTGTATG
TAAATCTTACGTTTCTCGCGTTGGTGAAGGCCCATTCCCAACTGAACAAATCAACGAAGTCGGTGATCGAATCCGTGAAA
TTGCTCATGAATATGGTACCGTTACTAAACGACCTCGTCGAATTGGATGGTTTGATGGCGTATTAATGAAATACGTTTCA
GAAGTTAATGGGCTAACTGATTTAGTTGTTAACTGTTTGGATGTCCTTTCAGGATTTAAAGAACTTAAAATCTGTACTGG
TTATCAAACTGATCACGGCGTAATTAAGTATTATCCTGCTTCAGAAAAAGAACTTAAAAATTCAACTCCAATTTATGAAA
CTCTTCCAGGTTGGGATGAAGATCTTACTCAAATGACTACTTACGAAGAATTACCAGCTAATGCTAAAAATTATCTTAAA
AAGATTGAAGAAATTACTGGAGTTCCAGTTAGCGCCTTTTCAATCGGCCCTGATCGTGAACAAACCATCGTCTTAAATGA
TATGTGGTCAGACTAA

Upstream 100 bases:

>100_bases
AGAATTACCTTTTGTATAATTCGGTTTATCCCTTTTATTTTTTTGGTAAACTGTGTTATCATCTTTAGAGTTGTGTATTG
ATAAAAATGGAGGAATAAAT

Downstream 100 bases:

>100_bases
AATAAAAAAAGAAGTAAAAATCATTAACGATTTTTACTTCTTTTTTTATTGACCTGATTAGCCCCAAAAGACAACACCAA
TAAAATGATTCCTAATATTA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTATVVIGSQWGDEGKGKIIDFLGQSADVTVRYSGGDNAGHSLVVNDQKLALRLIPSGILAPNSICIIGNGTVINPATLL
DEIKELNGVGVNTDHLKISDRAHIVFPYHILQDQQQERDRSKNGEKIGTTNKGIGPAYMDKMQRIGIRAIDLLDNETLEE
KIAFNLEQKKRILDEDLWNQLPSLKELTAQYIEYGEILKDYITDTSYLIHTNLNDNKRVLFEGAQGTMLDIDHGTYPFVT
SSNPTAGGAATGAGIGVTKIKHVIGVCKSYVSRVGEGPFPTEQINEVGDRIREIAHEYGTVTKRPRRIGWFDGVLMKYVS
EVNGLTDLVVNCLDVLSGFKELKICTGYQTDHGVIKYYPASEKELKNSTPIYETLPGWDEDLTQMTTYEELPANAKNYLK
KIEEITGVPVSAFSIGPDREQTIVLNDMWSD

Sequences:

>Translated_431_residues
MTATVVIGSQWGDEGKGKIIDFLGQSADVTVRYSGGDNAGHSLVVNDQKLALRLIPSGILAPNSICIIGNGTVINPATLL
DEIKELNGVGVNTDHLKISDRAHIVFPYHILQDQQQERDRSKNGEKIGTTNKGIGPAYMDKMQRIGIRAIDLLDNETLEE
KIAFNLEQKKRILDEDLWNQLPSLKELTAQYIEYGEILKDYITDTSYLIHTNLNDNKRVLFEGAQGTMLDIDHGTYPFVT
SSNPTAGGAATGAGIGVTKIKHVIGVCKSYVSRVGEGPFPTEQINEVGDRIREIAHEYGTVTKRPRRIGWFDGVLMKYVS
EVNGLTDLVVNCLDVLSGFKELKICTGYQTDHGVIKYYPASEKELKNSTPIYETLPGWDEDLTQMTTYEELPANAKNYLK
KIEEITGVPVSAFSIGPDREQTIVLNDMWSD
>Mature_430_residues
TATVVIGSQWGDEGKGKIIDFLGQSADVTVRYSGGDNAGHSLVVNDQKLALRLIPSGILAPNSICIIGNGTVINPATLLD
EIKELNGVGVNTDHLKISDRAHIVFPYHILQDQQQERDRSKNGEKIGTTNKGIGPAYMDKMQRIGIRAIDLLDNETLEEK
IAFNLEQKKRILDEDLWNQLPSLKELTAQYIEYGEILKDYITDTSYLIHTNLNDNKRVLFEGAQGTMLDIDHGTYPFVTS
SNPTAGGAATGAGIGVTKIKHVIGVCKSYVSRVGEGPFPTEQINEVGDRIREIAHEYGTVTKRPRRIGWFDGVLMKYVSE
VNGLTDLVVNCLDVLSGFKELKICTGYQTDHGVIKYYPASEKELKNSTPIYETLPGWDEDLTQMTTYEELPANAKNYLKK
IEEITGVPVSAFSIGPDREQTIVLNDMWSD

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=432, Percent_Identity=42.5925925925926, Blast_Score=335, Evalue=7e-92,
Organism=Homo sapiens, GI22748717, Length=430, Percent_Identity=41.8604651162791, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI40316944, Length=399, Percent_Identity=40.6015037593985, Blast_Score=284, Evalue=1e-76,
Organism=Escherichia coli, GI1790620, Length=425, Percent_Identity=44.2352941176471, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI25146553, Length=434, Percent_Identity=40.3225806451613, Blast_Score=307, Evalue=8e-84,
Organism=Caenorhabditis elegans, GI25146555, Length=434, Percent_Identity=40.3225806451613, Blast_Score=306, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6324109, Length=429, Percent_Identity=42.1911421911422, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=42.4242424242424, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PEDPA (Q03ET4)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804730.1
- ProteinModelPortal:   Q03ET4
- SMR:   Q03ET4
- STRING:   Q03ET4
- GeneID:   4417107
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1247
- NMPDR:   fig|278197.10.peg.1088
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- PhylomeDB:   Q03ET4
- BioCyc:   PPEN278197:PEPE_1247-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47724; Mature: 47592

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATVVIGSQWGDEGKGKIIDFLGQSADVTVRYSGGDNAGHSLVVNDQKLALRLIPSGIL
CCEEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEECHHEEEEECCCCCC
APNSICIIGNGTVINPATLLDEIKELNGVGVNTDHLKISDRAHIVFPYHILQDQQQERDR
CCCCEEEEECCCEECHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEHHHHCCHHHHHHH
SKNGEKIGTTNKGIGPAYMDKMQRIGIRAIDLLDNETLEEKIAFNLEQKKRILDEDLWNQ
HCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
LPSLKELTAQYIEYGEILKDYITDTSYLIHTNLNDNKRVLFEGAQGTMLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCEEEECCCCCCEEE
SSNPTAGGAATGAGIGVTKIKHVIGVCKSYVSRVGEGPFPTEQINEVGDRIREIAHEYGT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
VTKRPRRIGWFDGVLMKYVSEVNGLTDLVVNCLDVLSGFKELKICTGYQTDHGVIKYYPA
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEEECCC
SEKELKNSTPIYETLPGWDEDLTQMTTYEELPANAKNYLKKIEEITGVPVSAFSIGPDRE
CHHHHCCCCCCHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
QTIVLNDMWSD
CEEEEECCCCC
>Mature Secondary Structure 
TATVVIGSQWGDEGKGKIIDFLGQSADVTVRYSGGDNAGHSLVVNDQKLALRLIPSGIL
CEEEEEECCCCCCCCCHHHHHCCCCCCEEEEECCCCCCCCEEEEECHHEEEEECCCCCC
APNSICIIGNGTVINPATLLDEIKELNGVGVNTDHLKISDRAHIVFPYHILQDQQQERDR
CCCCEEEEECCCEECHHHHHHHHHHHCCCCCCCCEEEECCCEEEEEEHHHHCCHHHHHHH
SKNGEKIGTTNKGIGPAYMDKMQRIGIRAIDLLDNETLEEKIAFNLEQKKRILDEDLWNQ
HCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEHHCCHHHHHHHHCCHHHHHHHHHHHHHHH
LPSLKELTAQYIEYGEILKDYITDTSYLIHTNLNDNKRVLFEGAQGTMLDIDHGTYPFVT
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCCCEEEECCCCCCEEE
SSNPTAGGAATGAGIGVTKIKHVIGVCKSYVSRVGEGPFPTEQINEVGDRIREIAHEYGT
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCC
VTKRPRRIGWFDGVLMKYVSEVNGLTDLVVNCLDVLSGFKELKICTGYQTDHGVIKYYPA
CCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCEEEECCC
SEKELKNSTPIYETLPGWDEDLTQMTTYEELPANAKNYLKKIEEITGVPVSAFSIGPDRE
CHHHHCCCCCCHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
QTIVLNDMWSD
CEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA