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The map label for this gene is murD

Identifier: 116492948

GI number: 116492948

Start: 1187480

End: 1188847

Strand: Reverse

Name: murD

Synonym: PEPE_1187

Alternate gene names: 116492948

Gene position: 1188847-1187480 (Counterclockwise)

Preceding gene: 116492949

Following gene: 116492947

Centisome position: 64.88

GC content: 36.4

Gene sequence:

>1368_bases
ATGAAGTCAGTAGAAGATTTGGAAGATAAAAATGTTCTAGTTTTAGGAATGGCCAAGAGCGGAATAAGCGCCGCTCTTTT
ATTACATAGATTAAGAGCAAATGTTTTAGTTAATGATGCTAACGCTAATCAGCCACAAGAATTAATTGAAAAGCTTGAGA
ACAAAGGTATCCGGATGGTACTAGGGGAACATCCAACCAATATTTTAAGTCAAAATAAAATTGAGTTGATTGTTAAAAAT
CCGGGCATTCCTTATACAAATCCCGTTCTAGTCGACGCACAAGCCAGAGGTATAAAAATTGTTTCTGAACCTGAATTAGC
ATACTGGGTAATGGATAGTGAGTTGATCGGGGTTACAGGAAGTAACGGGAAAACAACCGTAACAACGCTAATTCAATTGA
TGCTTGACGATAATAAAAAACATGCTTATGTTGCCGGAAATATTGGAGTTCCTGCTACAACAGTTGCTCAAAAGGCCACT
GCAGAAGATAAAATAGTGATGGAATTATCTAGCTTTATGTTAGCTGGTATTGATCGACTTCATCCACATATTGCAGTTTT
GAATAACATTTTTGCTAGTCACTTAGACTGGCATAAAACACGTGAAAACTACGTTAATGACAAGATGAATATTACTAAGA
ATCAGACCAAGGACGATTTCTTGGTAATCAATTGGGATAATACTGAATGGCAAGAACTAGCTAAAAGAACTCAGGCGAAA
GTAGTTCCGTTTTCACGTCAAGGACTGACTAAGAGCGGTGCGTACGAAATTGAAGGCAAACTTTATTTTAAAGATGAATT
AATTATGGAAGCCGATGAAATTGGAATTCCTGGTGAACAAAATGTCGAAAATGCACTAGCCGCTATCGCAGTAGCTAAAA
TCGAAAAAGTCAGCACAGCTCATATTAAACAAGTTTTGACAACTTTTGGTGGAGTAAAACATCGAATTCAATATGTTGAA
AACCTCAATGGGCGTCAATTCTATAATGATTCTAAAGCGACTGATATCGAAGCTACCCAAGTAGCCTTACGTTCTTTTAA
AAAACCAGTGATATTAATTGCAGGTGGATTAGACCGACATGACGATTTAGATCGTTTAATTCCAGACTTAGAGCCAAATG
TAAAAGAAGTTATTGTTAATGGCGAAACTGCTGAGAAATTCAAAAAAATAGCTCAAAAAGCCGGTATTTCAGTAATTAAA
GATAGTTCTAGAGTAAGTGAATCGGTTGAAATAGCGTATCAAGATAGTAAAGAAGGGGATATTATCTTACTTTCACCGGC
AGCGGCTAGTTGGGATCAATACAAAACATTTGAAGAACGTGGTGACGAATTTATTGCAGCAGTTCAAAATTTACATAAAG
GGGAATAA

Upstream 100 bases:

>100_bases
TTAGGTTGGAGCGAATGGAAAGTAGATATTGTATTTTGGATAGTTGGTTTAATCGGATCCATAATTACGTTAATTAGTAT
TTTATAGGGAGTCTTTAATG

Downstream 100 bases:

>100_bases
TTAAATGAGACTGATGGTTTCTGGTGGTGGAACTGGTGGACATATTTATCCAGCCCTAGCACTGATTAAACAAGTTAAAC
AAAGTGAACCTGATTCACAG

Product: UDP-N-acetylmuramoylalanine--D-glutamate ligase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 455; Mature: 455

Protein sequence:

>455_residues
MKSVEDLEDKNVLVLGMAKSGISAALLLHRLRANVLVNDANANQPQELIEKLENKGIRMVLGEHPTNILSQNKIELIVKN
PGIPYTNPVLVDAQARGIKIVSEPELAYWVMDSELIGVTGSNGKTTVTTLIQLMLDDNKKHAYVAGNIGVPATTVAQKAT
AEDKIVMELSSFMLAGIDRLHPHIAVLNNIFASHLDWHKTRENYVNDKMNITKNQTKDDFLVINWDNTEWQELAKRTQAK
VVPFSRQGLTKSGAYEIEGKLYFKDELIMEADEIGIPGEQNVENALAAIAVAKIEKVSTAHIKQVLTTFGGVKHRIQYVE
NLNGRQFYNDSKATDIEATQVALRSFKKPVILIAGGLDRHDDLDRLIPDLEPNVKEVIVNGETAEKFKKIAQKAGISVIK
DSSRVSESVEIAYQDSKEGDIILLSPAAASWDQYKTFEERGDEFIAAVQNLHKGE

Sequences:

>Translated_455_residues
MKSVEDLEDKNVLVLGMAKSGISAALLLHRLRANVLVNDANANQPQELIEKLENKGIRMVLGEHPTNILSQNKIELIVKN
PGIPYTNPVLVDAQARGIKIVSEPELAYWVMDSELIGVTGSNGKTTVTTLIQLMLDDNKKHAYVAGNIGVPATTVAQKAT
AEDKIVMELSSFMLAGIDRLHPHIAVLNNIFASHLDWHKTRENYVNDKMNITKNQTKDDFLVINWDNTEWQELAKRTQAK
VVPFSRQGLTKSGAYEIEGKLYFKDELIMEADEIGIPGEQNVENALAAIAVAKIEKVSTAHIKQVLTTFGGVKHRIQYVE
NLNGRQFYNDSKATDIEATQVALRSFKKPVILIAGGLDRHDDLDRLIPDLEPNVKEVIVNGETAEKFKKIAQKAGISVIK
DSSRVSESVEIAYQDSKEGDIILLSPAAASWDQYKTFEERGDEFIAAVQNLHKGE
>Mature_455_residues
MKSVEDLEDKNVLVLGMAKSGISAALLLHRLRANVLVNDANANQPQELIEKLENKGIRMVLGEHPTNILSQNKIELIVKN
PGIPYTNPVLVDAQARGIKIVSEPELAYWVMDSELIGVTGSNGKTTVTTLIQLMLDDNKKHAYVAGNIGVPATTVAQKAT
AEDKIVMELSSFMLAGIDRLHPHIAVLNNIFASHLDWHKTRENYVNDKMNITKNQTKDDFLVINWDNTEWQELAKRTQAK
VVPFSRQGLTKSGAYEIEGKLYFKDELIMEADEIGIPGEQNVENALAAIAVAKIEKVSTAHIKQVLTTFGGVKHRIQYVE
NLNGRQFYNDSKATDIEATQVALRSFKKPVILIAGGLDRHDDLDRLIPDLEPNVKEVIVNGETAEKFKKIAQKAGISVIK
DSSRVSESVEIAYQDSKEGDIILLSPAAASWDQYKTFEERGDEFIAAVQNLHKGE

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=450, Percent_Identity=29.3333333333333, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_PEDPA (Q03EY1)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804683.1
- ProteinModelPortal:   Q03EY1
- SMR:   Q03EY1
- STRING:   Q03EY1
- GeneID:   4417586
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_1187
- NMPDR:   fig|278197.10.peg.1045
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q03EY1
- BioCyc:   PPEN278197:PEPE_1187-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50467; Mature: 50467

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSVEDLEDKNVLVLGMAKSGISAALLLHRLRANVLVNDANANQPQELIEKLENKGIRMV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHCCCEEEE
LGEHPTNILSQNKIELIVKNPGIPYTNPVLVDAQARGIKIVSEPELAYWVMDSELIGVTG
ECCCCCHHHCCCCEEEEEECCCCCCCCCEEEECCCCCEEEEECCCEEEEEECCEEEEEEC
SNGKTTVTTLIQLMLDDNKKHAYVAGNIGVPATTVAQKATAEDKIVMELSSFMLAGIDRL
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HPHIAVLNNIFASHLDWHKTRENYVNDKMNITKNQTKDDFLVINWDNTEWQELAKRTQAK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHE
VVPFSRQGLTKSGAYEIEGKLYFKDELIMEADEIGIPGEQNVENALAAIAVAKIEKVSTA
ECCCCCCCCCCCCCEEECCEEEECHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
HIKQVLTTFGGVKHRIQYVENLNGRQFYNDSKATDIEATQVALRSFKKPVILIAGGLDRH
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCC
DDLDRLIPDLEPNVKEVIVNGETAEKFKKIAQKAGISVIKDSSRVSESVEIAYQDSKEGD
CCHHHHCCCCCCCHHHEEECCCHHHHHHHHHHHCCCEEEECCHHHCCCEEEEEECCCCCC
IILLSPAAASWDQYKTFEERGDEFIAAVQNLHKGE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKSVEDLEDKNVLVLGMAKSGISAALLLHRLRANVLVNDANANQPQELIEKLENKGIRMV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHCCCEEEE
LGEHPTNILSQNKIELIVKNPGIPYTNPVLVDAQARGIKIVSEPELAYWVMDSELIGVTG
ECCCCCHHHCCCCEEEEEECCCCCCCCCEEEECCCCCEEEEECCCEEEEEECCEEEEEEC
SNGKTTVTTLIQLMLDDNKKHAYVAGNIGVPATTVAQKATAEDKIVMELSSFMLAGIDRL
CCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
HPHIAVLNNIFASHLDWHKTRENYVNDKMNITKNQTKDDFLVINWDNTEWQELAKRTQAK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHE
VVPFSRQGLTKSGAYEIEGKLYFKDELIMEADEIGIPGEQNVENALAAIAVAKIEKVSTA
ECCCCCCCCCCCCCEEECCEEEECHHHHEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
HIKQVLTTFGGVKHRIQYVENLNGRQFYNDSKATDIEATQVALRSFKKPVILIAGGLDRH
HHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCCC
DDLDRLIPDLEPNVKEVIVNGETAEKFKKIAQKAGISVIKDSSRVSESVEIAYQDSKEGD
CCHHHHCCCCCCCHHHEEECCCHHHHHHHHHHHCCCEEEECCHHHCCCEEEEEECCCCCC
IILLSPAAASWDQYKTFEERGDEFIAAVQNLHKGE
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA