Click here to switch to the map view.

The map label for this gene is prs2 [H]

Identifier: 116492929

GI number: 116492929

Start: 1167759

End: 1168736

Strand: Reverse

Name: prs2 [H]

Synonym: PEPE_1168

Alternate gene names: 116492929

Gene position: 1168736-1167759 (Counterclockwise)

Preceding gene: 116492930

Following gene: 116492928

Centisome position: 63.78

GC content: 34.97

Gene sequence:

>978_bases
GTGGAAGAAAGTAAAACAAAAAGAAGAATGAAAATATTCTCCTTGAATTCAAATCATGCTTTATCTGAAAAAATTGCTGC
AGAGGTGGGAATGCCTTTAGGAAAAGCTGCTATTAAGCAATTTAGTGATGGCGAAATCCAAGTAAATATTGAAGAAAGTA
TTCGCGGGGATGAAGTTTATGTAATTCAATCCATTTCAGACCCTATTAATGATTCATTGATGGAATTATTAATTATGGTT
GATGCTTTGCGTCGTGCTAGTGCTAGCCAAATTAATGTTGTTATTCCATATTATGGATACTCTAGACAGGATCGCAAAGC
TCGTTCACGGGAACCAATTACAGCTAAATTAATTGCTAATCTTTTGGAAGATGATGCAATTACTCGTGTTCTGACGCTTG
ATTTACATGCTCCACAAATTCAAGGATTTTTCGATGTTCCAGTTGACCATCTTGCCGCTGCTCCATTGTTAGCAAGTTAT
TTTAGTGATGGGAGTTTTGATGTCGAAAATATGGTTGTAGTTTCACCTGATCACGCTAGTGTTTCTAGAGCTAGAACCAT
GGCTGAATTATTAGGCACACCCATTGCAATTATTGATAATCGTAATGAAGAATCTGTGGAAAGTATTAATGAAATTCCGA
CTGAAATCATTGGAAATGTTAAAAATAAAATTGCTTTGGTTGTCGATGATATGATTGACACAGGAACACGCTTGACGATT
TCAGCGGAAGCCCTCCATAACGCAGGAGCAACTGAGGTATATGGTGCTGCAACTCATGCAATCTTTTCTAATAATGCACC
TAAAATTTTACAAGAATCCAAATTGTCTAAAATCATTGTTACTGATTCAATTAGAATTGATGCTGATAAGAAATTTGATA
AGTTAGTTGAATTATCTGTAGGACCACTTTTAGGAAATGCAATTAAGATGATTTATGATAATGAACCACTTGCTCCATTA
TTTAAAAGCCAAAAATAA

Upstream 100 bases:

>100_bases
TGGAAGGGGTCACACCGTATGATTTTATTTAGTTAATATCTTGAAGTGTGTAGCCTAAGTTGTCTATAATGATAGGTAGA
TAATAATTGGAGGTTCAGCT

Downstream 100 bases:

>100_bases
TTAAATAAGTGTTAAATAACTTTTTAAATTTTCGATTAAGATCTGTTGCAGATTATAATTTGTGCAGGTCTTTTTATTTG
GAGGAATTCAATGGATATAT

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK 2; Phosphoribosyl pyrophosphate synthase 2; P-Rib-PP synthase 2; PRPP synthase 2 [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MEESKTKRRMKIFSLNSNHALSEKIAAEVGMPLGKAAIKQFSDGEIQVNIEESIRGDEVYVIQSISDPINDSLMELLIMV
DALRRASASQINVVIPYYGYSRQDRKARSREPITAKLIANLLEDDAITRVLTLDLHAPQIQGFFDVPVDHLAAAPLLASY
FSDGSFDVENMVVVSPDHASVSRARTMAELLGTPIAIIDNRNEESVESINEIPTEIIGNVKNKIALVVDDMIDTGTRLTI
SAEALHNAGATEVYGAATHAIFSNNAPKILQESKLSKIIVTDSIRIDADKKFDKLVELSVGPLLGNAIKMIYDNEPLAPL
FKSQK

Sequences:

>Translated_325_residues
MEESKTKRRMKIFSLNSNHALSEKIAAEVGMPLGKAAIKQFSDGEIQVNIEESIRGDEVYVIQSISDPINDSLMELLIMV
DALRRASASQINVVIPYYGYSRQDRKARSREPITAKLIANLLEDDAITRVLTLDLHAPQIQGFFDVPVDHLAAAPLLASY
FSDGSFDVENMVVVSPDHASVSRARTMAELLGTPIAIIDNRNEESVESINEIPTEIIGNVKNKIALVVDDMIDTGTRLTI
SAEALHNAGATEVYGAATHAIFSNNAPKILQESKLSKIIVTDSIRIDADKKFDKLVELSVGPLLGNAIKMIYDNEPLAPL
FKSQK
>Mature_325_residues
MEESKTKRRMKIFSLNSNHALSEKIAAEVGMPLGKAAIKQFSDGEIQVNIEESIRGDEVYVIQSISDPINDSLMELLIMV
DALRRASASQINVVIPYYGYSRQDRKARSREPITAKLIANLLEDDAITRVLTLDLHAPQIQGFFDVPVDHLAAAPLLASY
FSDGSFDVENMVVVSPDHASVSRARTMAELLGTPIAIIDNRNEESVESINEIPTEIIGNVKNKIALVVDDMIDTGTRLTI
SAEALHNAGATEVYGAATHAIFSNNAPKILQESKLSKIIVTDSIRIDADKKFDKLVELSVGPLLGNAIKMIYDNEPLAPL
FKSQK

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=40.4458598726115, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=40.4458598726115, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI4506129, Length=312, Percent_Identity=39.4230769230769, Blast_Score=236, Evalue=2e-62,
Organism=Homo sapiens, GI84875539, Length=315, Percent_Identity=39.0476190476191, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI4506133, Length=350, Percent_Identity=36, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=34.593023255814, Blast_Score=189, Evalue=4e-48,
Organism=Homo sapiens, GI310128524, Length=140, Percent_Identity=32.8571428571429, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310115209, Length=140, Percent_Identity=32.8571428571429, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310118259, Length=140, Percent_Identity=32.8571428571429, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI310119946, Length=140, Percent_Identity=32.8571428571429, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI1787458, Length=316, Percent_Identity=45.5696202531646, Blast_Score=266, Evalue=1e-72,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=39.4904458598726, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17554702, Length=322, Percent_Identity=39.1304347826087, Blast_Score=233, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI71989924, Length=322, Percent_Identity=39.1304347826087, Blast_Score=232, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17554704, Length=312, Percent_Identity=39.4230769230769, Blast_Score=231, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=30.1775147928994, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6319403, Length=315, Percent_Identity=40, Blast_Score=229, Evalue=5e-61,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=39.6825396825397, Blast_Score=225, Evalue=9e-60,
Organism=Saccharomyces cerevisiae, GI6321776, Length=317, Percent_Identity=35.6466876971609, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6322667, Length=206, Percent_Identity=40.7766990291262, Blast_Score=150, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324511, Length=92, Percent_Identity=39.1304347826087, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355239, Length=314, Percent_Identity=39.4904458598726, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI45551540, Length=337, Percent_Identity=37.0919881305638, Blast_Score=221, Evalue=4e-58,
Organism=Drosophila melanogaster, GI281362873, Length=353, Percent_Identity=32.0113314447592, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24651454, Length=353, Percent_Identity=32.0113314447592, Blast_Score=182, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24651458, Length=353, Percent_Identity=32.0113314447592, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI24651456, Length=353, Percent_Identity=32.0113314447592, Blast_Score=181, Evalue=4e-46,
Organism=Drosophila melanogaster, GI45552010, Length=372, Percent_Identity=30.6451612903226, Blast_Score=161, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24651462, Length=372, Percent_Identity=30.6451612903226, Blast_Score=160, Evalue=8e-40,
Organism=Drosophila melanogaster, GI24651464, Length=372, Percent_Identity=30.6451612903226, Blast_Score=160, Evalue=8e-40,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35642; Mature: 35642

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEESKTKRRMKIFSLNSNHALSEKIAAEVGMPLGKAAIKQFSDGEIQVNIEESIRGDEVY
CCCCHHHHEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEHHCCCCCEEE
VIQSISDPINDSLMELLIMVDALRRASASQINVVIPYYGYSRQDRKARSREPITAKLIAN
EEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHH
LLEDDAITRVLTLDLHAPQIQGFFDVPVDHLAAAPLLASYFSDGSFDVENMVVVSPDHAS
HHHHHHHEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
VSRARTMAELLGTPIAIIDNRNEESVESINEIPTEIIGNVKNKIALVVDDMIDTGTRLTI
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCHHHHHCCHHHHEEEEEEHHHCCCCEEEE
SAEALHNAGATEVYGAATHAIFSNNAPKILQESKLSKIIVTDSIRIDADKKFDKLVELSV
EHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHC
GPLLGNAIKMIYDNEPLAPLFKSQK
CHHHHHHHHEEECCCCCCHHHCCCC
>Mature Secondary Structure
MEESKTKRRMKIFSLNSNHALSEKIAAEVGMPLGKAAIKQFSDGEIQVNIEESIRGDEVY
CCCCHHHHEEEEEEECCCCHHHHHHHHHHCCCCCHHHHHCCCCCEEEEEEHHCCCCCEEE
VIQSISDPINDSLMELLIMVDALRRASASQINVVIPYYGYSRQDRKARSREPITAKLIAN
EEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHHHHH
LLEDDAITRVLTLDLHAPQIQGFFDVPVDHLAAAPLLASYFSDGSFDVENMVVVSPDHAS
HHHHHHHEEEEEEECCCCCCCCEECCCHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHH
VSRARTMAELLGTPIAIIDNRNEESVESINEIPTEIIGNVKNKIALVVDDMIDTGTRLTI
HHHHHHHHHHHCCCEEEEECCCHHHHHHHHHCHHHHHCCHHHHEEEEEEHHHCCCCEEEE
SAEALHNAGATEVYGAATHAIFSNNAPKILQESKLSKIIVTDSIRIDADKKFDKLVELSV
EHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHCCCEEEEECCEEECCCHHHHHHHHHHC
GPLLGNAIKMIYDNEPLAPLFKSQK
CHHHHHHHHEEECCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12566566 [H]