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The map label for this gene is nusA [H]

Identifier: 116492653

GI number: 116492653

Start: 889410

End: 890498

Strand: Direct

Name: nusA [H]

Synonym: PEPE_0887

Alternate gene names: 116492653

Gene position: 889410-890498 (Clockwise)

Preceding gene: 116492652

Following gene: 116492654

Centisome position: 48.54

GC content: 38.48

Gene sequence:

>1089_bases
ATGAGTAAAGAAATGATTACCGCTCTTGATATCTTAGAAAAAGAAAAGGGAATCAAAAAAGAAGTTATCGTTGAAGCACT
TGAAGCTGCATTAGTTTCGGCATTCAAGCGTAATTACGGACAGGCTCAAAACGTTGAAGTAGAATTTAATGATCGTAAAG
GTGATATTCATGTTTACGCCGTTAAAAAAGTTGTTGAAGAAGTTGAAGATGATCAATTGGAAGTTAGTTTAGCTGACGCC
TTGATGATCAATCGAGCATACGAATTGGATGATGAAATCAGATTTGAAGTTACTCCTAAAAACTTTGGCCGAATTGCAGC
TCAAACTGCTAAACAAGTTATTATGCAACGGGTTCGCGAAGCTGAACGTGGTGTAATTTACGAAAAGTATGGTCAATACG
AAGATGAAATTGTTACAGGCGAAGTATCACACCGTGACAATCGTTACGTATATGTTGACTTGGGTGGGGTCGAAGCTGTG
ATGGCTAAGTCAGACCAAATCCCAGGTGAACAATATGAACCTCATGATCATATTAAAGTTTATATTACGAAGGTGGAAAA
TAACGGTAAGGGACCTCAAATTTTTGCTTCAAGAACGGATTCGGGACTTTTGAAACGGCTCTTTGAACAAGAAGTTCCTG
AAGTTTATGATGGAACGGTCGAGATTATGTCGATTGCTCGTGAAGCTGGTGATCGTGCTAAAATTGCGGTACGTTCTAAT
GATCCTGATATTGATGCAGTTGGGACATGTGTGGGTGCTAAGGGCCAACGTGTTCAAACGATTGTCAATGAACTTCATGG
TGAAAACATGGATATTATTGAATGGAAAGAGGACCCAGCAGAATATATAGCTAATGCTTTGAAACCTTCTGAAGTTATTG
ACGTATTATTTAATGAAGAAGATGAAAATTACTGCATGGTAATCGTTCCAGATTACCAGCTTTCATTAGCAATTGGTCGT
CGTGGACAAAATGCGCGTTTAGCTGCTAAACTTGTTAACTTCAAGATTGACATCAAATCGGAAACAGATGCATCAGAATA
TGCTGAAGAAGAAGATTCTGAAGAAAGTACAGAAGAAACCGCTGAATAA

Upstream 100 bases:

>100_bases
TTGGACTATTTAGATAAGACTAGACATAAAGAACTTAAAGTAACGTATGATCAGGTTGCATTTGCTAGATTAGCAATCAA
GATTTAGAGGAGAGTTTAAA

Downstream 100 bases:

>100_bases
ACCATTGGAGGTGTGTTCTTGATGAAAACCAGAAAAATACCAATGCGTAAAGACATTGTGACAAATCAAATGTTTCCTAA
AAAGGAACTCGTGCGAGTTG

Product: transcription elongation factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MSKEMITALDILEKEKGIKKEVIVEALEAALVSAFKRNYGQAQNVEVEFNDRKGDIHVYAVKKVVEEVEDDQLEVSLADA
LMINRAYELDDEIRFEVTPKNFGRIAAQTAKQVIMQRVREAERGVIYEKYGQYEDEIVTGEVSHRDNRYVYVDLGGVEAV
MAKSDQIPGEQYEPHDHIKVYITKVENNGKGPQIFASRTDSGLLKRLFEQEVPEVYDGTVEIMSIAREAGDRAKIAVRSN
DPDIDAVGTCVGAKGQRVQTIVNELHGENMDIIEWKEDPAEYIANALKPSEVIDVLFNEEDENYCMVIVPDYQLSLAIGR
RGQNARLAAKLVNFKIDIKSETDASEYAEEEDSEESTEETAE

Sequences:

>Translated_362_residues
MSKEMITALDILEKEKGIKKEVIVEALEAALVSAFKRNYGQAQNVEVEFNDRKGDIHVYAVKKVVEEVEDDQLEVSLADA
LMINRAYELDDEIRFEVTPKNFGRIAAQTAKQVIMQRVREAERGVIYEKYGQYEDEIVTGEVSHRDNRYVYVDLGGVEAV
MAKSDQIPGEQYEPHDHIKVYITKVENNGKGPQIFASRTDSGLLKRLFEQEVPEVYDGTVEIMSIAREAGDRAKIAVRSN
DPDIDAVGTCVGAKGQRVQTIVNELHGENMDIIEWKEDPAEYIANALKPSEVIDVLFNEEDENYCMVIVPDYQLSLAIGR
RGQNARLAAKLVNFKIDIKSETDASEYAEEEDSEESTEETAE
>Mature_361_residues
SKEMITALDILEKEKGIKKEVIVEALEAALVSAFKRNYGQAQNVEVEFNDRKGDIHVYAVKKVVEEVEDDQLEVSLADAL
MINRAYELDDEIRFEVTPKNFGRIAAQTAKQVIMQRVREAERGVIYEKYGQYEDEIVTGEVSHRDNRYVYVDLGGVEAVM
AKSDQIPGEQYEPHDHIKVYITKVENNGKGPQIFASRTDSGLLKRLFEQEVPEVYDGTVEIMSIAREAGDRAKIAVRSND
PDIDAVGTCVGAKGQRVQTIVNELHGENMDIIEWKEDPAEYIANALKPSEVIDVLFNEEDENYCMVIVPDYQLSLAIGRR
GQNARLAAKLVNFKIDIKSETDASEYAEEEDSEESTEETAE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=344, Percent_Identity=37.5, Blast_Score=243, Evalue=1e-65,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 40821; Mature: 40690

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMITALDILEKEKGIKKEVIVEALEAALVSAFKRNYGQAQNVEVEFNDRKGDIHVYA
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEH
VKKVVEEVEDDQLEVSLADALMINRAYELDDEIRFEVTPKNFGRIAAQTAKQVIMQRVRE
HHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AERGVIYEKYGQYEDEIVTGEVSHRDNRYVYVDLGGVEAVMAKSDQIPGEQYEPHDHIKV
HHCCCEEHHCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEE
YITKVENNGKGPQIFASRTDSGLLKRLFEQEVPEVYDGTVEIMSIAREAGDRAKIAVRSN
EEEEEECCCCCCEEEECCCCHHHHHHHHHHHCHHHHCCHHHHHHHHHHCCCCEEEEEECC
DPDIDAVGTCVGAKGQRVQTIVNELHGENMDIIEWKEDPAEYIANALKPSEVIDVLFNEE
CCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHCCCC
DENYCMVIVPDYQLSLAIGRRGQNARLAAKLVNFKIDIKSETDASEYAEEEDSEESTEET
CCCEEEEEECCCEEEEEECCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHH
AE
CC
>Mature Secondary Structure 
SKEMITALDILEKEKGIKKEVIVEALEAALVSAFKRNYGQAQNVEVEFNDRKGDIHVYA
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCEEEEH
VKKVVEEVEDDQLEVSLADALMINRAYELDDEIRFEVTPKNFGRIAAQTAKQVIMQRVRE
HHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHH
AERGVIYEKYGQYEDEIVTGEVSHRDNRYVYVDLGGVEAVMAKSDQIPGEQYEPHDHIKV
HHCCCEEHHCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHCCCCCCCCCCCCCCCEEE
YITKVENNGKGPQIFASRTDSGLLKRLFEQEVPEVYDGTVEIMSIAREAGDRAKIAVRSN
EEEEEECCCCCCEEEECCCCHHHHHHHHHHHCHHHHCCHHHHHHHHHHCCCCEEEEEECC
DPDIDAVGTCVGAKGQRVQTIVNELHGENMDIIEWKEDPAEYIANALKPSEVIDVLFNEE
CCCHHHHHHCCCCCCHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHHHHHCCCC
DENYCMVIVPDYQLSLAIGRRGQNARLAAKLVNFKIDIKSETDASEYAEEEDSEESTEET
CCCEEEEEECCCEEEEEECCCCCCHHHEEEEEEEEEEECCCCCHHHHHHHCCCHHHHHHH
AE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]