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The map label for this gene is fmt

Identifier: 116492596

GI number: 116492596

Start: 834842

End: 835804

Strand: Direct

Name: fmt

Synonym: PEPE_0829

Alternate gene names: 116492596

Gene position: 834842-835804 (Clockwise)

Preceding gene: 116492595

Following gene: 116492597

Centisome position: 45.56

GC content: 38.42

Gene sequence:

>963_bases
ATGAAATCAATTATTTTTATGGGAACACCAGAATTTTCGGCGCCAATTTTGCAAAGCCTAATTGACGAGCCAAACTATGA
TGTGATTGGGGTAGTTACCCAACCGGATCGCAAAGTCGGGCGAAAACATGTTTTAACACCCAGTCCAGTAAAAAAAGTTG
CTGTTAAAAACGATATTAAGGTTTATCAACCAGAGAAGTTAAGTGGAAGTGCTGAATTGACAGAACTAATCGCGTTAAAT
GCGGATTTAATTGTCACGGCCGCTTTTGGTCAATTTCTTCCAATGAGTTTGATTAACTCCGTTAAGATTGGGGCAGTCAA
TGTGCATGCTTCATTATTACCAAAATATCGTGGAGGAGCACCCGTGCACTATGCCATAATGAATGGCGACAAAGAAACTG
GTGTTACTATTATCTATATGGTTAAAAAAATGGATGCCGGTGAAATGCTTGCTACTGCAAAAATTCCTATTACCGACCAA
GATGATGTGGGAACCATGTTTGAAAAGTTAAGTCTTTTAGGGCGTGATTTATTGTTAGACACGCTACCTCAATTAATTGA
AGGAAATGTTCAACCAGTTAAACAAGATGAAGAACAAGTTAGCTTTTCGCCTAATATTAGCCCCGAAGAAGAAGAAATTG
ATATTAATTTACCGGCCCGTCTAGTAGATGCAAAGGTCCGCGGATTACGACCTTTCCCAGTAGCCTACTTTATGATGGAA
GGCAAACGAACTAAGATATGGAAAACAAAAGTAATAGATCAAACTACAGATTTAAAACCGGGTCAAGTTGTTTCAAAAAC
GAAGCATGAGCTCTTGGTAGCAACTGGAGAACACGGCGTAATTAGTATTGAAGAATTACAGCCAGCTGGCAAACAAAAGA
TGACAATTACAGACTATCTCAATGGAGTAGGTGAAAACTTACACCCAGGAAAGCAAATAATAGATAATGACAAATCAAAA
TAA

Upstream 100 bases:

>100_bases
GTATTGAACAAATCGCAGGAGAAATTTAGACAGGGGACACGGATTAGTATTGATTCCGAACCACAAAACTTTTTGTAATA
TTTAGGAAGTAGGTCTAATT

Downstream 100 bases:

>100_bases
TAGTGCACGAAATTTAGCAGTTGATATTTTAGAAAAGATTGATGAAGGTTCTTTTTCCAATATTCAACTTAATCAAGATA
TAAAAAGTTCATCATTGATT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MKSIIFMGTPEFSAPILQSLIDEPNYDVIGVVTQPDRKVGRKHVLTPSPVKKVAVKNDIKVYQPEKLSGSAELTELIALN
ADLIVTAAFGQFLPMSLINSVKIGAVNVHASLLPKYRGGAPVHYAIMNGDKETGVTIIYMVKKMDAGEMLATAKIPITDQ
DDVGTMFEKLSLLGRDLLLDTLPQLIEGNVQPVKQDEEQVSFSPNISPEEEEIDINLPARLVDAKVRGLRPFPVAYFMME
GKRTKIWKTKVIDQTTDLKPGQVVSKTKHELLVATGEHGVISIEELQPAGKQKMTITDYLNGVGENLHPGKQIIDNDKSK

Sequences:

>Translated_320_residues
MKSIIFMGTPEFSAPILQSLIDEPNYDVIGVVTQPDRKVGRKHVLTPSPVKKVAVKNDIKVYQPEKLSGSAELTELIALN
ADLIVTAAFGQFLPMSLINSVKIGAVNVHASLLPKYRGGAPVHYAIMNGDKETGVTIIYMVKKMDAGEMLATAKIPITDQ
DDVGTMFEKLSLLGRDLLLDTLPQLIEGNVQPVKQDEEQVSFSPNISPEEEEIDINLPARLVDAKVRGLRPFPVAYFMME
GKRTKIWKTKVIDQTTDLKPGQVVSKTKHELLVATGEHGVISIEELQPAGKQKMTITDYLNGVGENLHPGKQIIDNDKSK
>Mature_320_residues
MKSIIFMGTPEFSAPILQSLIDEPNYDVIGVVTQPDRKVGRKHVLTPSPVKKVAVKNDIKVYQPEKLSGSAELTELIALN
ADLIVTAAFGQFLPMSLINSVKIGAVNVHASLLPKYRGGAPVHYAIMNGDKETGVTIIYMVKKMDAGEMLATAKIPITDQ
DDVGTMFEKLSLLGRDLLLDTLPQLIEGNVQPVKQDEEQVSFSPNISPEEEEIDINLPARLVDAKVRGLRPFPVAYFMME
GKRTKIWKTKVIDQTTDLKPGQVVSKTKHELLVATGEHGVISIEELQPAGKQKMTITDYLNGVGENLHPGKQIIDNDKSK

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=333, Percent_Identity=28.5285285285285, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI21614513, Length=249, Percent_Identity=24.8995983935743, Blast_Score=85, Evalue=8e-17,
Organism=Homo sapiens, GI238814322, Length=300, Percent_Identity=22.3333333333333, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1789683, Length=312, Percent_Identity=41.025641025641, Blast_Score=247, Evalue=9e-67,
Organism=Escherichia coli, GI1788589, Length=278, Percent_Identity=28.7769784172662, Blast_Score=133, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI133930964, Length=266, Percent_Identity=25.187969924812, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI45550868, Length=305, Percent_Identity=29.8360655737705, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI28571984, Length=225, Percent_Identity=30.6666666666667, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24585660, Length=240, Percent_Identity=27.0833333333333, Blast_Score=89, Evalue=3e-18,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_PEDPA (Q03FY3)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804331.1
- ProteinModelPortal:   Q03FY3
- SMR:   Q03FY3
- STRING:   Q03FY3
- GeneID:   4417981
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0829
- NMPDR:   fig|278197.10.peg.733
- eggNOG:   COG0223
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- PhylomeDB:   Q03FY3
- ProtClustDB:   CLSK581455
- BioCyc:   PPEN278197:PEPE_0829-MONOMER
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 35161; Mature: 35161

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIIFMGTPEFSAPILQSLIDEPNYDVIGVVTQPDRKVGRKHVLTPSPVKKVAVKNDIK
CCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHCCCCE
VYQPEKLSGSAELTELIALNADLIVTAAFGQFLPMSLINSVKIGAVNVHASLLPKYRGGA
EECCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCEEEEEEEEHHHHCCCCCCCC
PVHYAIMNGDKETGVTIIYMVKKMDAGEMLATAKIPITDQDDVGTMFEKLSLLGRDLLLD
CEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
TLPQLIEGNVQPVKQDEEQVSFSPNISPEEEEIDINLPARLVDAKVRGLRPFPVAYFMME
HHHHHHCCCCCCCCCCCHHEEECCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEEEEC
GKRTKIWKTKVIDQTTDLKPGQVVSKTKHELLVATGEHGVISIEELQPAGKQKMTITDYL
CCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCCEEEHHHCCCCCCCCEEHHHHH
NGVGENLHPGKQIIDNDKSK
HHCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MKSIIFMGTPEFSAPILQSLIDEPNYDVIGVVTQPDRKVGRKHVLTPSPVKKVAVKNDIK
CCCEEEEECCCCCHHHHHHHHCCCCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHCCCCE
VYQPEKLSGSAELTELIALNADLIVTAAFGQFLPMSLINSVKIGAVNVHASLLPKYRGGA
EECCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHCCEEEEEEEEHHHHCCCCCCCC
PVHYAIMNGDKETGVTIIYMVKKMDAGEMLATAKIPITDQDDVGTMFEKLSLLGRDLLLD
CEEEEEECCCCCCCEEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
TLPQLIEGNVQPVKQDEEQVSFSPNISPEEEEIDINLPARLVDAKVRGLRPFPVAYFMME
HHHHHHCCCCCCCCCCCHHEEECCCCCCCCCEEEECCCHHHHHHHHCCCCCCCEEEEEEC
GKRTKIWKTKVIDQTTDLKPGQVVSKTKHELLVATGEHGVISIEELQPAGKQKMTITDYL
CCCCEEEEEECCCCCCCCCCCHHHHCCCCEEEEEECCCCEEEHHHCCCCCCCCEEHHHHH
NGVGENLHPGKQIIDNDKSK
HHCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA