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The map label for this gene is priA [H]

Identifier: 116492595

GI number: 116492595

Start: 832399

End: 834819

Strand: Direct

Name: priA [H]

Synonym: PEPE_0828

Alternate gene names: 116492595

Gene position: 832399-834819 (Clockwise)

Preceding gene: 116492594

Following gene: 116492596

Centisome position: 45.43

GC content: 37.71

Gene sequence:

>2421_bases
ATGGCAAAAAAATTTAATGTCATCGTAAATGTCCCAGCTTTACAAACTAATACACCATTTACATATATCGCAAATGATGC
CATTAAAAATGACATTAAAATTGGTTCCAGAGTAATTGTTCCTTTTGGTAAGTCTGAGCGACCTATCCAAGGTTTTGTGG
TCGGAGAAGAAACCACTAATGAATTTGAAGGATTGAAGGACATCATTTCAGTTTTAGAACCGGAACCATTGCTAAATAAA
GAATTGATTGAGCTTTCAGAGTGGTTAGCCGACCAAACGTATGCTTTTCGAGTAAGTTGTTTAAATGCAATGCTTCCAAG
TTTGGTGAAAGTTAATACCAAGCGTTTTATTAAAATCAATGAAACAAAAATTACAATGGAAACCGTGCTTGCCTTATTAG
GGGAAGAACAGGTTACAGATCTTAAAGTAAATGAACTCAGTTTTAGCCAACAAAAACAACTTAATCAGTGGATTAAAGAT
GAATTCGTTACATTAAAATATGTCACGGAAGATTCTGCTAAAAAGAAAATGATTAATTGGCTTTGGCCTACGATGGATTT
AAGTGAGTATGAACAAGAACAAAAAAAGTTAAGAAAAAATGCTACGAAACAAGTTGAGTTACTAAATATAATTAAAACGT
TAGATGTAAAAAAGGGAATTTCGCAAGCTTCAATTTTAAATGATTATGGTGTAGGACGCTCAACATTAAATACAGCGGTT
AAAAATGGATGGCTAGTTCGTAAGGCGGTTGAGCAATATCGCGATCCTTTTGAAGGACGTCAGATGCAGCTTGATCAAAA
TTTGGCTTTAAATGAAGAACAGCAATCCGCTTACAACTTAATTACACAAGCAATTGAAGACAAAAATGCTAAACCAATTT
TAGTTGAAGGCGTTACTGGATCGGGAAAAACAGAAGTTTATTTGCAGAGTATCCAACAGGTAATAAAAAAAGGGAAAACT
GCTCTTTTACTGGTTCCAGAAATTTCCTTAACACCTTTAATGGTGAAAAGGGTTCGGGCGCGTTTTGGTACACAAGTGGC
TGTTCTCCATAGTGGGCTTTCTGACGGTGAAAAGTTTGATGAATGGCGAAGAATTGTACGAAATGAAGTTAAAGTAGTAG
TTGGAGCACGGTCGGCGGTCTTTGCACCACTACGTAATATTGGTATTATAATTATTGATGAGGAACATTCAGAAACGTAT
AAGCAATCTGATGCACCGCGTTATCATGCTCGGGATGTAGCGATTCATCGTGCAAAGAAATTTGAATGTCCCGTAGTCTT
AGGAAGCGCTACTCCTTCTCTTGAGAGTCGTTCTCGCGTGGAACGTGGGCGATACGATTTTGTAAAAATTCAAAAACGAG
CCAATCAAAAGGCTTTACCGAATATTCAAGTGATCGATATGCGTCAGCATTTAGGTGATGGATATAATGAAAACTTTTCG
GCACCCATGGTTGAAGAAATTCGACATCGGATTTTAAGGAAAGAACAAGTAGTTTTAATGTTGAATCGGCGAGGTTTTTC
ATCCTTCGTGATGTGTCGAACGTGTGGTTTTGTCCTAACTTGTCCTAACTGTGATGTATCTTTAACGATGCATATGGATT
CTCGAACCATGCGGTGTCATTATTGTGGTCATGAAGAAGCAATCCCACGTTTATGTAAAAATTGTGGTAGTGATAAAATT
CGGTTTTTCGGTACTGGAACTGAAAAAGTTCAACAGCAATTGGCAGAGATCATTCCTGAAGCTCGCGTACTTCGGATGGA
TGTTGATACCACCCGTCGCAAGGGAGCCCATGAGCGTCTATTAGAACGTTTTGGCAACCACGAGGCAGATATTCTTTTGG
GTACGCAAATGATTGCTAAGGGGTTGGATTTTCCTGATGTAACCTTGGTAGGAGTTTTAAATGCGGATACAGCACTAGAA
TTACCGGATTTACGGGCAAGTGAGCGAACTTTCCAGCTTTTAACTCAGGTTGCTGGACGTGCTGGACGTGCTGATAAGAC
CGGTGAAGTACTAATTCAAACCTTTAACCCCGATCACTATGCAATTCGTTTAGCTCAAAAACAAGATTATGAAGACTTTT
TCTATAAAGAAATGTATTTGAGGCATTTAGGCAAATATCCGCCTTATTACTACACGGTATTAGTTAGTGTTTCAGATCGG
GATGAAAGACAAGCGTTAAAAAATGCTTACGAAATGTTGAATATTATTCAACCGCAGTTATCAGCTAATGCAATTGTGTT
AGGGCCGACGGCTAAATCTATCTCAAAAATTAAACAAAAATATTACTACCAAATTTTAATTAAATATCAAAGAGAGCCTA
AATTACATCGTGCGCTGTTAGAAGTATTGAACAAATCGCAGGAGAAATTTAGACAGGGGACACGGATTAGTATTGATTCC
GAACCACAAAACTTTTTGTAA

Upstream 100 bases:

>100_bases
ACCAAGCTAATATAGAATTGAAAAAGAATGATAAATCGATAATTGCTGAACAGATTTTAAAGCTAATCGACCAAGAAATT
AATTGAAAGGAACGTAACTC

Downstream 100 bases:

>100_bases
TATTTAGGAAGTAGGTCTAATTATGAAATCAATTATTTTTATGGGAACACCAGAATTTTCGGCGCCAATTTTGCAAAGCC
TAATTGACGAGCCAAACTAT

Product: replication restart DNA helicase PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MAKKFNVIVNVPALQTNTPFTYIANDAIKNDIKIGSRVIVPFGKSERPIQGFVVGEETTNEFEGLKDIISVLEPEPLLNK
ELIELSEWLADQTYAFRVSCLNAMLPSLVKVNTKRFIKINETKITMETVLALLGEEQVTDLKVNELSFSQQKQLNQWIKD
EFVTLKYVTEDSAKKKMINWLWPTMDLSEYEQEQKKLRKNATKQVELLNIIKTLDVKKGISQASILNDYGVGRSTLNTAV
KNGWLVRKAVEQYRDPFEGRQMQLDQNLALNEEQQSAYNLITQAIEDKNAKPILVEGVTGSGKTEVYLQSIQQVIKKGKT
ALLLVPEISLTPLMVKRVRARFGTQVAVLHSGLSDGEKFDEWRRIVRNEVKVVVGARSAVFAPLRNIGIIIIDEEHSETY
KQSDAPRYHARDVAIHRAKKFECPVVLGSATPSLESRSRVERGRYDFVKIQKRANQKALPNIQVIDMRQHLGDGYNENFS
APMVEEIRHRILRKEQVVLMLNRRGFSSFVMCRTCGFVLTCPNCDVSLTMHMDSRTMRCHYCGHEEAIPRLCKNCGSDKI
RFFGTGTEKVQQQLAEIIPEARVLRMDVDTTRRKGAHERLLERFGNHEADILLGTQMIAKGLDFPDVTLVGVLNADTALE
LPDLRASERTFQLLTQVAGRAGRADKTGEVLIQTFNPDHYAIRLAQKQDYEDFFYKEMYLRHLGKYPPYYYTVLVSVSDR
DERQALKNAYEMLNIIQPQLSANAIVLGPTAKSISKIKQKYYYQILIKYQREPKLHRALLEVLNKSQEKFRQGTRISIDS
EPQNFL

Sequences:

>Translated_806_residues
MAKKFNVIVNVPALQTNTPFTYIANDAIKNDIKIGSRVIVPFGKSERPIQGFVVGEETTNEFEGLKDIISVLEPEPLLNK
ELIELSEWLADQTYAFRVSCLNAMLPSLVKVNTKRFIKINETKITMETVLALLGEEQVTDLKVNELSFSQQKQLNQWIKD
EFVTLKYVTEDSAKKKMINWLWPTMDLSEYEQEQKKLRKNATKQVELLNIIKTLDVKKGISQASILNDYGVGRSTLNTAV
KNGWLVRKAVEQYRDPFEGRQMQLDQNLALNEEQQSAYNLITQAIEDKNAKPILVEGVTGSGKTEVYLQSIQQVIKKGKT
ALLLVPEISLTPLMVKRVRARFGTQVAVLHSGLSDGEKFDEWRRIVRNEVKVVVGARSAVFAPLRNIGIIIIDEEHSETY
KQSDAPRYHARDVAIHRAKKFECPVVLGSATPSLESRSRVERGRYDFVKIQKRANQKALPNIQVIDMRQHLGDGYNENFS
APMVEEIRHRILRKEQVVLMLNRRGFSSFVMCRTCGFVLTCPNCDVSLTMHMDSRTMRCHYCGHEEAIPRLCKNCGSDKI
RFFGTGTEKVQQQLAEIIPEARVLRMDVDTTRRKGAHERLLERFGNHEADILLGTQMIAKGLDFPDVTLVGVLNADTALE
LPDLRASERTFQLLTQVAGRAGRADKTGEVLIQTFNPDHYAIRLAQKQDYEDFFYKEMYLRHLGKYPPYYYTVLVSVSDR
DERQALKNAYEMLNIIQPQLSANAIVLGPTAKSISKIKQKYYYQILIKYQREPKLHRALLEVLNKSQEKFRQGTRISIDS
EPQNFL
>Mature_805_residues
AKKFNVIVNVPALQTNTPFTYIANDAIKNDIKIGSRVIVPFGKSERPIQGFVVGEETTNEFEGLKDIISVLEPEPLLNKE
LIELSEWLADQTYAFRVSCLNAMLPSLVKVNTKRFIKINETKITMETVLALLGEEQVTDLKVNELSFSQQKQLNQWIKDE
FVTLKYVTEDSAKKKMINWLWPTMDLSEYEQEQKKLRKNATKQVELLNIIKTLDVKKGISQASILNDYGVGRSTLNTAVK
NGWLVRKAVEQYRDPFEGRQMQLDQNLALNEEQQSAYNLITQAIEDKNAKPILVEGVTGSGKTEVYLQSIQQVIKKGKTA
LLLVPEISLTPLMVKRVRARFGTQVAVLHSGLSDGEKFDEWRRIVRNEVKVVVGARSAVFAPLRNIGIIIIDEEHSETYK
QSDAPRYHARDVAIHRAKKFECPVVLGSATPSLESRSRVERGRYDFVKIQKRANQKALPNIQVIDMRQHLGDGYNENFSA
PMVEEIRHRILRKEQVVLMLNRRGFSSFVMCRTCGFVLTCPNCDVSLTMHMDSRTMRCHYCGHEEAIPRLCKNCGSDKIR
FFGTGTEKVQQQLAEIIPEARVLRMDVDTTRRKGAHERLLERFGNHEADILLGTQMIAKGLDFPDVTLVGVLNADTALEL
PDLRASERTFQLLTQVAGRAGRADKTGEVLIQTFNPDHYAIRLAQKQDYEDFFYKEMYLRHLGKYPPYYYTVLVSVSDRD
ERQALKNAYEMLNIIQPQLSANAIVLGPTAKSISKIKQKYYYQILIKYQREPKLHRALLEVLNKSQEKFRQGTRISIDSE
PQNFL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=507, Percent_Identity=41.2228796844181, Blast_Score=411, Evalue=1e-116,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 92141; Mature: 92009

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKFNVIVNVPALQTNTPFTYIANDAIKNDIKIGSRVIVPFGKSERPIQGFVVGEETTN
CCCEEEEEEECCCEECCCCEEEEECHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCH
EFEGLKDIISVLEPEPLLNKELIELSEWLADQTYAFRVSCLNAMLPSLVKVNTKRFIKIN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
ETKITMETVLALLGEEQVTDLKVNELSFSQQKQLNQWIKDEFVTLKYVTEDSAKKKMINW
CCCHHHHHHHHHHCCCHHCCEEEHHCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHH
LWPTMDLSEYEQEQKKLRKNATKQVELLNIIKTLDVKKGISQASILNDYGVGRSTLNTAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHH
KNGWLVRKAVEQYRDPFEGRQMQLDQNLALNEEQQSAYNLITQAIEDKNAKPILVEGVTG
HCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCC
SGKTEVYLQSIQQVIKKGKTALLLVPEISLTPLMVKRVRARFGTQVAVLHSGLSDGEKFD
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEHHCCCCCCHHHH
EWRRIVRNEVKVVVGARSAVFAPLRNIGIIIIDEEHSETYKQSDAPRYHARDVAIHRAKK
HHHHHHHHHHEEEEECCHHHHHHHHHCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCC
FECPVVLGSATPSLESRSRVERGRYDFVKIQKRANQKALPNIQVIDMRQHLGDGYNENFS
CCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCCCC
APMVEEIRHRILRKEQVVLMLNRRGFSSFVMCRTCGFVLTCPNCDVSLTMHMDSRTMRCH
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCEEEECCCCCEEEEEEECCCEEEEE
YCGHEEAIPRLCKNCGSDKIRFFGTGTEKVQQQLAEIIPEARVLRMDVDTTRRKGAHERL
ECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCHHHHHCCHHHHH
LERFGNHEADILLGTQMIAKGLDFPDVTLVGVLNADTALELPDLRASERTFQLLTQVAGR
HHHHCCCCCEEEEHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
AGRADKTGEVLIQTFNPDHYAIRLAQKQDYEDFFYKEMYLRHLGKYPPYYYTVLVSVSDR
CCCCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
DERQALKNAYEMLNIIQPQLSANAIVLGPTAKSISKIKQKYYYQILIKYQREPKLHRALL
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EVLNKSQEKFRQGTRISIDSEPQNFL
HHHHCHHHHHHCCCEEEECCCCCCCC
>Mature Secondary Structure 
AKKFNVIVNVPALQTNTPFTYIANDAIKNDIKIGSRVIVPFGKSERPIQGFVVGEETTN
CCEEEEEEECCCEECCCCEEEEECHHHHHHHCCCCEEEEECCCCCCCCEEEEECCCCCH
EFEGLKDIISVLEPEPLLNKELIELSEWLADQTYAFRVSCLNAMLPSLVKVNTKRFIKIN
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEC
ETKITMETVLALLGEEQVTDLKVNELSFSQQKQLNQWIKDEFVTLKYVTEDSAKKKMINW
CCCHHHHHHHHHHCCCHHCCEEEHHCCCHHHHHHHHHHHHCCEEEEEEECCHHHHHHHHH
LWPTMDLSEYEQEQKKLRKNATKQVELLNIIKTLDVKKGISQASILNDYGVGRSTLNTAV
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHH
KNGWLVRKAVEQYRDPFEGRQMQLDQNLALNEEQQSAYNLITQAIEDKNAKPILVEGVTG
HCCHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCC
SGKTEVYLQSIQQVIKKGKTALLLVPEISLTPLMVKRVRARFGTQVAVLHSGLSDGEKFD
CCCHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHCCEEEEHHCCCCCCHHHH
EWRRIVRNEVKVVVGARSAVFAPLRNIGIIIIDEEHSETYKQSDAPRYHARDVAIHRAKK
HHHHHHHHHHEEEEECCHHHHHHHHHCCEEEEECCCCHHHHHCCCCCHHHHHHHHHHHCC
FECPVVLGSATPSLESRSRVERGRYDFVKIQKRANQKALPNIQVIDMRQHLGDGYNENFS
CCCCEEECCCCCCHHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCCCC
APMVEEIRHRILRKEQVVLMLNRRGFSSFVMCRTCGFVLTCPNCDVSLTMHMDSRTMRCH
CHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHCCEEEECCCCCEEEEEEECCCEEEEE
YCGHEEAIPRLCKNCGSDKIRFFGTGTEKVQQQLAEIIPEARVLRMDVDTTRRKGAHERL
ECCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHCCCCEEEEECCHHHHHCCHHHHH
LERFGNHEADILLGTQMIAKGLDFPDVTLVGVLNADTALELPDLRASERTFQLLTQVAGR
HHHHCCCCCEEEEHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCC
AGRADKTGEVLIQTFNPDHYAIRLAQKQDYEDFFYKEMYLRHLGKYPPYYYTVLVSVSDR
CCCCCCCCEEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
DERQALKNAYEMLNIIQPQLSANAIVLGPTAKSISKIKQKYYYQILIKYQREPKLHRALL
HHHHHHHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
EVLNKSQEKFRQGTRISIDSEPQNFL
HHHHCHHHHHHCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]