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The map label for this gene is dnaB [H]

Identifier: 116492528

GI number: 116492528

Start: 787956

End: 789203

Strand: Direct

Name: dnaB [H]

Synonym: PEPE_0760

Alternate gene names: 116492528

Gene position: 787956-789203 (Clockwise)

Preceding gene: 116492527

Following gene: 116492529

Centisome position: 43.0

GC content: 41.75

Gene sequence:

>1248_bases
ATGAACACAGAGATTGAAAAAGAAGTGATCGGGATTTTGCTGAACCACCCAGAGAAACTATCTGGAATCCAGCTGGAATC
AGATTGGTTCGGCTATCCCGAATATCAAACCCTATTCGAAGCCATGCACCAATCCGTCAGCCACGATCTGTTCGATGTGT
ACGGAAAATATAATCAACTATCTAAGCAGCCCATGGCTTTCAACATTGTCAAAGACATCAGAAATGAATCGCCGATGGTT
AGTTTACTCAATAACGATATCCAATTAATCCGTAAAGCAGCGTACACACGTCAATTGAATATTGCGATTGACAAATATCA
GACGAATCCATTTAGCGAAAATGAGCAGGCTTTGCGGGAAGTACTAGCCAAAAATGCATCGGTTGAAACGATTGATGATG
GGTTACTGGATGAAGCAGTGGAAGAGTTGAAAGATTCGTTGACACATGCTCGACCACGAGGCGTGAAGACGTTTAACAAA
TTAGATACGGCGCTCGGTGGTGGAATGTACGGATCCATGTTGTTCACGATTGGAGCACGTCCATCAACTGGTAAGACGGC
ATTCTCGGTAAATCTGGCGTACGAAGCATTAATGAATGACAAAGAAGTTGAAATAGATTTCTTTACGCTCGAAATGAACA
AGCAAGAAATGCTCAATCGTTTTATCTCCAGAATGACTGGTGTTTCAAGCAGTACTCTACGTTCAAAAGCTAACGAACTG
AACGATATTCTGAAAACTCTAATCGATCAAGCTACAAGCCAGTTATTGGCGTCTAAATTGCGTGTGTACGACGGTTTAGA
GACTCTCGGTGAAATTGTCCAGACGATACGCAAAAACGCTGCTAAGGCGAAGCAGGGTAAGTATATTGCGATCATTGATT
ACATTGGACTGGTTAAGGTACCAAACGTGAAAGATCGGTATATCGAAGTCGGAGAAGTCACTAGAGAGCTTAAACGACTA
ACGAACGAGTTTAATATCCCAATAGTGGCGTTGTCTCAATTGTCACGAGGTATTGAAAATCGGCAAGATAAGACACCGTT
ACTATCTGATCTAAGAGAATCAGGAAGCATTGAACAAGATTCAAACGTAGTGGCATTTCTACACCGTCCACAAGGGATAA
AAAGTGAACGGGTAGTACAACTATCGATTCGAAAGAATCGTGAAGGTGAGTTGGCGGATATTGACTTTACATTTATCGGT
GAAGAAATGTTGTTTCTGGAATATGAGCCCAAAGAGGTGAAAAACTAA

Upstream 100 bases:

>100_bases
AGCGATGGTCAACGTCAAGGTAAGACAACAGAAGAAATTGAAGCGGAACGTAAAAGGCATGAAGAGGAAGTAACAAGAAG
AGCAGAGGAGAGACTGAATA

Downstream 100 bases:

>100_bases
CTATGGCATACATGGATTACTACGAGTATCAAGCCATTATGAAAGAAAACGGATATGTTGAATCAGAAGCTGTGAAGATA
TTTTTAAAACGAGCAGCAGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MNTEIEKEVIGILLNHPEKLSGIQLESDWFGYPEYQTLFEAMHQSVSHDLFDVYGKYNQLSKQPMAFNIVKDIRNESPMV
SLLNNDIQLIRKAAYTRQLNIAIDKYQTNPFSENEQALREVLAKNASVETIDDGLLDEAVEELKDSLTHARPRGVKTFNK
LDTALGGGMYGSMLFTIGARPSTGKTAFSVNLAYEALMNDKEVEIDFFTLEMNKQEMLNRFISRMTGVSSSTLRSKANEL
NDILKTLIDQATSQLLASKLRVYDGLETLGEIVQTIRKNAAKAKQGKYIAIIDYIGLVKVPNVKDRYIEVGEVTRELKRL
TNEFNIPIVALSQLSRGIENRQDKTPLLSDLRESGSIEQDSNVVAFLHRPQGIKSERVVQLSIRKNREGELADIDFTFIG
EEMLFLEYEPKEVKN

Sequences:

>Translated_415_residues
MNTEIEKEVIGILLNHPEKLSGIQLESDWFGYPEYQTLFEAMHQSVSHDLFDVYGKYNQLSKQPMAFNIVKDIRNESPMV
SLLNNDIQLIRKAAYTRQLNIAIDKYQTNPFSENEQALREVLAKNASVETIDDGLLDEAVEELKDSLTHARPRGVKTFNK
LDTALGGGMYGSMLFTIGARPSTGKTAFSVNLAYEALMNDKEVEIDFFTLEMNKQEMLNRFISRMTGVSSSTLRSKANEL
NDILKTLIDQATSQLLASKLRVYDGLETLGEIVQTIRKNAAKAKQGKYIAIIDYIGLVKVPNVKDRYIEVGEVTRELKRL
TNEFNIPIVALSQLSRGIENRQDKTPLLSDLRESGSIEQDSNVVAFLHRPQGIKSERVVQLSIRKNREGELADIDFTFIG
EEMLFLEYEPKEVKN
>Mature_415_residues
MNTEIEKEVIGILLNHPEKLSGIQLESDWFGYPEYQTLFEAMHQSVSHDLFDVYGKYNQLSKQPMAFNIVKDIRNESPMV
SLLNNDIQLIRKAAYTRQLNIAIDKYQTNPFSENEQALREVLAKNASVETIDDGLLDEAVEELKDSLTHARPRGVKTFNK
LDTALGGGMYGSMLFTIGARPSTGKTAFSVNLAYEALMNDKEVEIDFFTLEMNKQEMLNRFISRMTGVSSSTLRSKANEL
NDILKTLIDQATSQLLASKLRVYDGLETLGEIVQTIRKNAAKAKQGKYIAIIDYIGLVKVPNVKDRYIEVGEVTRELKRL
TNEFNIPIVALSQLSRGIENRQDKTPLLSDLRESGSIEQDSNVVAFLHRPQGIKSERVVQLSIRKNREGELADIDFTFIG
EEMLFLEYEPKEVKN

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=252, Percent_Identity=36.5079365079365, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 47026; Mature: 47026

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTEIEKEVIGILLNHPEKLSGIQLESDWFGYPEYQTLFEAMHQSVSHDLFDVYGKYNQL
CCCHHHHHHHHHHHCCCCHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKQPMAFNIVKDIRNESPMVSLLNNDIQLIRKAAYTRQLNIAIDKYQTNPFSENEQALRE
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHH
VLAKNASVETIDDGLLDEAVEELKDSLTHARPRGVKTFNKLDTALGGGMYGSMLFTIGAR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHEECCC
PSTGKTAFSVNLAYEALMNDKEVEIDFFTLEMNKQEMLNRFISRMTGVSSSTLRSKANEL
CCCCCEEEEEHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHH
NDILKTLIDQATSQLLASKLRVYDGLETLGEIVQTIRKNAAKAKQGKYIAIIDYIGLVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEC
PNVKDRYIEVGEVTRELKRLTNEFNIPIVALSQLSRGIENRQDKTPLLSDLRESGSIEQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
SNVVAFLHRPQGIKSERVVQLSIRKNREGELADIDFTFIGEEMLFLEYEPKEVKN
CCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCEEEEEEECHHHCCC
>Mature Secondary Structure
MNTEIEKEVIGILLNHPEKLSGIQLESDWFGYPEYQTLFEAMHQSVSHDLFDVYGKYNQL
CCCHHHHHHHHHHHCCCCHHCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SKQPMAFNIVKDIRNESPMVSLLNNDIQLIRKAAYTRQLNIAIDKYQTNPFSENEQALRE
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCCCCHHHHHH
VLAKNASVETIDDGLLDEAVEELKDSLTHARPRGVKTFNKLDTALGGGMYGSMLFTIGAR
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHEECCC
PSTGKTAFSVNLAYEALMNDKEVEIDFFTLEMNKQEMLNRFISRMTGVSSSTLRSKANEL
CCCCCEEEEEHHHHHHHCCCCCEEEEEEEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHH
NDILKTLIDQATSQLLASKLRVYDGLETLGEIVQTIRKNAAKAKQGKYIAIIDYIGLVKV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCEEC
PNVKDRYIEVGEVTRELKRLTNEFNIPIVALSQLSRGIENRQDKTPLLSDLRESGSIEQD
CCCHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHCCCCCCHHHHHHHHCCCCCCC
SNVVAFLHRPQGIKSERVVQLSIRKNREGELADIDFTFIGEEMLFLEYEPKEVKN
CCEEEEEECCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCEEEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]