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The map label for this gene is pheS [H]

Identifier: 116492489

GI number: 116492489

Start: 757981

End: 759048

Strand: Direct

Name: pheS [H]

Synonym: PEPE_0721

Alternate gene names: 116492489

Gene position: 757981-759048 (Clockwise)

Preceding gene: 116492488

Following gene: 116492490

Centisome position: 41.37

GC content: 39.04

Gene sequence:

>1068_bases
TTGGAGAAAGGTGGAAAGATCATGAGTTTACAAGAGAAGTTAAAAGAACTTGAAGAAAAAGGTCTTCAAGAAATTAGCGT
TGCTAATGAATTATCAAAATTAAATGAAGTACGGGTCAATTTTTTAGGTAAAAAAGGTCCGATTACTGAGGTCCTCCGAG
GGATGCGTGATTTAAGTAAAGAGGAACGTCCTAAGGTCGGTGAATTTGCTAATAAAGTACGTGATAACCTAACATCTAAA
TTGGAAGAACGTAAAGAAGAATTAGCACAGGCAGCCTTGAATAAAAAATTAGCTGAAGAGGCTGTAGATGTTACTTTGCC
AGGGACACCGATGAAGCAAGGCCAACCGCATGTTATTCAGCAAATTATTGATAACTTGGAAGATTTATTCCTTGGGATGG
GTTACCAAGTTGTTGAAGGACCAGAAGTTGAACTCGACCGCTATAACTTTGAGATGCTAAATCTGCCTAAAGATCATCCG
GCTCGCGATATGCAAGATACATTTTACATCACGGACGAAGTTTTGATGCGCTCTCAAACTTCTCCAGTTCAAGCTCGAAC
AATGGAAAAACATGACTTTTCAAAGGGACCATTAAAAATGATCTCACCAGGGGTAGTTTACCGTCGCGATACTGATGATG
CTACTCATTCACACCAATTCCATCAAATTGAAGGATTAGTGATTGATAAGAACATTACGATGGGTGATTTGAAAGGTACT
TTAGTAACTTTGGCTAAGGAACTTTTTGGTGATCGTTTCGAAGTACGCTTGCGTCCAAGTTATTTTCCATTTACAGAACC
TTCTGTTGAAGCAGACGTTACTTGCTTTAATTGTATGGGAAAAGGCTGTTCAGTATGTAAGTCAACCGGTTGGATAGAAG
TATTAGGGGCTGGAATGGTTCATCCTAATGTTTTGAAAATGGCTGGTGTGGACACGGACGTTTACGGTGGTTTTGCCTTT
GGATTAGGACCTGATCGTTTTGCAATGTTACGTTATGGTGTTGATGATATTCGTAATTTCTACCTAAATGATGTTAGATT
CCTATCCCAATTCAATAAGCGAGGTTAA

Upstream 100 bases:

>100_bases
GCAGGCCACCCGTATCTGGCTGACCAAGTGTGCATTGAGTTGCAAACAAGGGTGGTACCGCGTTAAAAAACGTCCCTTTC
TAGGAGGGGCGTTTTTTTAT

Downstream 100 bases:

>100_bases
TAAAAAATGAAACTTTCATACAAGTGGTTATCTAAATATTTAGAACTAGACTTAGAACCTAAAGACTTGGCTGAAAAGAT
TGAACGTACAGCTGTTGAAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MEKGGKIMSLQEKLKELEEKGLQEISVANELSKLNEVRVNFLGKKGPITEVLRGMRDLSKEERPKVGEFANKVRDNLTSK
LEERKEELAQAALNKKLAEEAVDVTLPGTPMKQGQPHVIQQIIDNLEDLFLGMGYQVVEGPEVELDRYNFEMLNLPKDHP
ARDMQDTFYITDEVLMRSQTSPVQARTMEKHDFSKGPLKMISPGVVYRRDTDDATHSHQFHQIEGLVIDKNITMGDLKGT
LVTLAKELFGDRFEVRLRPSYFPFTEPSVEADVTCFNCMGKGCSVCKSTGWIEVLGAGMVHPNVLKMAGVDTDVYGGFAF
GLGPDRFAMLRYGVDDIRNFYLNDVRFLSQFNKRG

Sequences:

>Translated_355_residues
MEKGGKIMSLQEKLKELEEKGLQEISVANELSKLNEVRVNFLGKKGPITEVLRGMRDLSKEERPKVGEFANKVRDNLTSK
LEERKEELAQAALNKKLAEEAVDVTLPGTPMKQGQPHVIQQIIDNLEDLFLGMGYQVVEGPEVELDRYNFEMLNLPKDHP
ARDMQDTFYITDEVLMRSQTSPVQARTMEKHDFSKGPLKMISPGVVYRRDTDDATHSHQFHQIEGLVIDKNITMGDLKGT
LVTLAKELFGDRFEVRLRPSYFPFTEPSVEADVTCFNCMGKGCSVCKSTGWIEVLGAGMVHPNVLKMAGVDTDVYGGFAF
GLGPDRFAMLRYGVDDIRNFYLNDVRFLSQFNKRG
>Mature_355_residues
MEKGGKIMSLQEKLKELEEKGLQEISVANELSKLNEVRVNFLGKKGPITEVLRGMRDLSKEERPKVGEFANKVRDNLTSK
LEERKEELAQAALNKKLAEEAVDVTLPGTPMKQGQPHVIQQIIDNLEDLFLGMGYQVVEGPEVELDRYNFEMLNLPKDHP
ARDMQDTFYITDEVLMRSQTSPVQARTMEKHDFSKGPLKMISPGVVYRRDTDDATHSHQFHQIEGLVIDKNITMGDLKGT
LVTLAKELFGDRFEVRLRPSYFPFTEPSVEADVTCFNCMGKGCSVCKSTGWIEVLGAGMVHPNVLKMAGVDTDVYGGFAF
GLGPDRFAMLRYGVDDIRNFYLNDVRFLSQFNKRG

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=238, Percent_Identity=33.1932773109244, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI4758340, Length=275, Percent_Identity=29.4545454545455, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI1788007, Length=338, Percent_Identity=47.3372781065089, Blast_Score=318, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI32566635, Length=252, Percent_Identity=34.1269841269841, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI32563657, Length=277, Percent_Identity=27.4368231046931, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=277, Percent_Identity=27.4368231046931, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321087, Length=351, Percent_Identity=26.7806267806268, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6325304, Length=282, Percent_Identity=28.7234042553192, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI18858079, Length=297, Percent_Identity=28.956228956229, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=29.9212598425197, Blast_Score=112, Evalue=3e-25,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 40089; Mature: 40089

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKGGKIMSLQEKLKELEEKGLQEISVANELSKLNEVRVNFLGKKGPITEVLRGMRDLSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EERPKVGEFANKVRDNLTSKLEERKEELAQAALNKKLAEEAVDVTLPGTPMKQGQPHVIQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHH
QIIDNLEDLFLGMGYQVVEGPEVELDRYNFEMLNLPKDHPARDMQDTFYITDEVLMRSQT
HHHHHHHHHHHCCCCEEECCCCCEEECCCEEEECCCCCCCCCCCCHHEEEHHHHHHHCCC
SPVQARTMEKHDFSKGPLKMISPGVVYRRDTDDATHSHQFHQIEGLVIDKNITMGDLKGT
CCHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCHHCCHHHHCCCEEEECCCCHHHHHHH
LVTLAKELFGDRFEVRLRPSYFPFTEPSVEADVTCFNCMGKGCSVCKSTGWIEVLGAGMV
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCHHHHHCCCCC
HPNVLKMAGVDTDVYGGFAFGLGPDRFAMLRYGVDDIRNFYLNDVRFLSQFNKRG
CCCHHHHCCCCCCCCCCEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKGGKIMSLQEKLKELEEKGLQEISVANELSKLNEVRVNFLGKKGPITEVLRGMRDLSK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
EERPKVGEFANKVRDNLTSKLEERKEELAQAALNKKLAEEAVDVTLPGTPMKQGQPHVIQ
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHH
QIIDNLEDLFLGMGYQVVEGPEVELDRYNFEMLNLPKDHPARDMQDTFYITDEVLMRSQT
HHHHHHHHHHHCCCCEEECCCCCEEECCCEEEECCCCCCCCCCCCHHEEEHHHHHHHCCC
SPVQARTMEKHDFSKGPLKMISPGVVYRRDTDDATHSHQFHQIEGLVIDKNITMGDLKGT
CCHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCHHCCHHHHCCCEEEECCCCHHHHHHH
LVTLAKELFGDRFEVRLRPSYFPFTEPSVEADVTCFNCMGKGCSVCKSTGWIEVLGAGMV
HHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHCCCHHHHHCCCHHHHHCCCCC
HPNVLKMAGVDTDVYGGFAFGLGPDRFAMLRYGVDDIRNFYLNDVRFLSQFNKRG
CCCHHHHCCCCCCCCCCEEECCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA