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The map label for this gene is ysgA [H]

Identifier: 116492486

GI number: 116492486

Start: 756000

End: 756767

Strand: Direct

Name: ysgA [H]

Synonym: PEPE_0718

Alternate gene names: 116492486

Gene position: 756000-756767 (Clockwise)

Preceding gene: 116492483

Following gene: 116492487

Centisome position: 41.26

GC content: 36.72

Gene sequence:

>768_bases
ATGGAAAAGATTTCTTCAACAAAGAATTCAAAAGTGAAAGAATGGGTGAAACTAAAAACCAATAATGGTAGAAAAAAATT
TAAGAAATTTCTTTTAGAAGGACGTCATCCAGTGGAGACGGCTTTGGAACAGGCAACTGAATATGAGTTTTTAATTGTCC
AAGAAGACTTTGATTTAGGTGAGCTTGCTGAACAAGTCGATAATGACAAATTAATTTTAGTGACCAAAGAAGTTGCTAAT
CATATTTCAGATACTGTTCACGATCAAGGCATCTTTTTAATTGGTTACATTAACGAGGAAGATTGGCAATTTCCAGATGA
TTTGTCTGGAAAATGGTTAGTTTTGGATAATGTACAAGATCCTGGAAATATTGGTACAATGGTAAGAACGGCTGATGCGG
CTGGGTTTACAGGAGTTGTATTTGGCGATGGGACAGCTGATTTCTATAACCCTAAGATTCTAAGGGCTATGCAGGGTAGT
CAGTATCACTTGCAGTTGAAACGATTGAATCTTGAGAAATGGATTCGTAATTGTAAGGATCAAGGTAGTGTAATTTTTGG
CTCAATTTTAGATGAAAATGCCCGTGACTATCGTAAAATTGATGTTCCAGACCAGTTCTCTTTGATTATGGGGAATGAAG
GCAACGGCATGAGTGAAAGACTTATTGAAATGACGGATATGAATTTATATATTCCAATCAAGGGTTCCGCAGAATCTTTG
AATGTGGCGGTTGCTGCTGGAGTATTAATGTTTGGTTTAGCCGGTTAA

Upstream 100 bases:

>100_bases
AAGTTCGGCAAGTCTGTATATCATTTAGATTGTGATAATGATACGATTAAATAGGATAGAAACAGAATACTTACAAATGG
TAAGGAGATAAGGTATAATT

Downstream 100 bases:

>100_bases
GGTTTTGTTACGTTTTAAATTTGGCAGTTAGATTTACTTGATTTAAAAAAGTATGACGCTAAGATGGTAGTAACTTAAGC
TAAAAAGGAGCTTTTTAATG

Product: rRNA methylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MEKISSTKNSKVKEWVKLKTNNGRKKFKKFLLEGRHPVETALEQATEYEFLIVQEDFDLGELAEQVDNDKLILVTKEVAN
HISDTVHDQGIFLIGYINEEDWQFPDDLSGKWLVLDNVQDPGNIGTMVRTADAAGFTGVVFGDGTADFYNPKILRAMQGS
QYHLQLKRLNLEKWIRNCKDQGSVIFGSILDENARDYRKIDVPDQFSLIMGNEGNGMSERLIEMTDMNLYIPIKGSAESL
NVAVAAGVLMFGLAG

Sequences:

>Translated_255_residues
MEKISSTKNSKVKEWVKLKTNNGRKKFKKFLLEGRHPVETALEQATEYEFLIVQEDFDLGELAEQVDNDKLILVTKEVAN
HISDTVHDQGIFLIGYINEEDWQFPDDLSGKWLVLDNVQDPGNIGTMVRTADAAGFTGVVFGDGTADFYNPKILRAMQGS
QYHLQLKRLNLEKWIRNCKDQGSVIFGSILDENARDYRKIDVPDQFSLIMGNEGNGMSERLIEMTDMNLYIPIKGSAESL
NVAVAAGVLMFGLAG
>Mature_255_residues
MEKISSTKNSKVKEWVKLKTNNGRKKFKKFLLEGRHPVETALEQATEYEFLIVQEDFDLGELAEQVDNDKLILVTKEVAN
HISDTVHDQGIFLIGYINEEDWQFPDDLSGKWLVLDNVQDPGNIGTMVRTADAAGFTGVVFGDGTADFYNPKILRAMQGS
QYHLQLKRLNLEKWIRNCKDQGSVIFGSILDENARDYRKIDVPDQFSLIMGNEGNGMSERLIEMTDMNLYIPIKGSAESL
NVAVAAGVLMFGLAG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=236, Percent_Identity=28.8135593220339, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1790083, Length=141, Percent_Identity=29.0780141843972, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28639; Mature: 28639

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKISSTKNSKVKEWVKLKTNNGRKKFKKFLLEGRHPVETALEQATEYEFLIVQEDFDLG
CCCCCCCCCHHHHHHEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHH
ELAEQVDNDKLILVTKEVANHISDTVHDQGIFLIGYINEEDWQFPDDLSGKWLVLDNVQD
HHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCC
PGNIGTMVRTADAAGFTGVVFGDGTADFYNPKILRAMQGSQYHLQLKRLNLEKWIRNCKD
CCCCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEEEECCHHHHHHHHCC
QGSVIFGSILDENARDYRKIDVPDQFSLIMGNEGNGMSERLIEMTDMNLYIPIKGSAESL
CCCEEEEEHHCCCCCCCEECCCCCCEEEEECCCCCCHHHHHHEEECCEEEEEECCCCCHH
NVAVAAGVLMFGLAG
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKISSTKNSKVKEWVKLKTNNGRKKFKKFLLEGRHPVETALEQATEYEFLIVQEDFDLG
CCCCCCCCCHHHHHHEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCEEEEEEECCCCHH
ELAEQVDNDKLILVTKEVANHISDTVHDQGIFLIGYINEEDWQFPDDLSGKWLVLDNVQD
HHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCC
PGNIGTMVRTADAAGFTGVVFGDGTADFYNPKILRAMQGSQYHLQLKRLNLEKWIRNCKD
CCCCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHHHCCCEEEEEEEECCHHHHHHHHCC
QGSVIFGSILDENARDYRKIDVPDQFSLIMGNEGNGMSERLIEMTDMNLYIPIKGSAESL
CCCEEEEEHHCCCCCCCEECCCCCCEEEEECCCCCCHHHHHHEEECCEEEEEECCCCCHH
NVAVAAGVLMFGLAG
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]