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The map label for this gene is dnaB [H]

Identifier: 116492460

GI number: 116492460

Start: 732854

End: 734224

Strand: Direct

Name: dnaB [H]

Synonym: PEPE_0692

Alternate gene names: 116492460

Gene position: 732854-734224 (Clockwise)

Preceding gene: 116492459

Following gene: 116492461

Centisome position: 39.99

GC content: 37.05

Gene sequence:

>1371_bases
ATGGCAACTGAGATGAGTGAATTAGCACCCCATGATTCATTTATAATTGATAGCCAACGTCAAATTACTGATTTTGAGCA
ACGTTCATTGACGACACTATATCGTCCATTGTTGACCTCAGATGCATATAGTTTGATGATGGTTTTGTGGGAAATGAGTC
AGGAAACCAAATCGATCTCAGAACAGTACACACATACAATCCTTTTAAACTGGCTTGGAATCGATCTACCAGCGATAGTT
GAGGCACGAGGACGTTTAGAAGCACTAGGTCTACTAAAAACGTATCAGAGAAAAATTGATCGTCGAAGTTTATTTTTGTA
TGCACTCCAAGCTCCAGCCGATCCCGTTAAATTCTTTAAAGATGACACTTTGAGTGCTTTATTGCTGGGCGTGATTGGCG
AAAGTGAATTCGATCGGCTGAGTCATCGACTGATTAAGCAGCACGTGCGTCAGGACGAATTTTATGATGTTTCCAAAAAG
CTGGGCGACTATTTTCAATTGGGAAGTAGCGTAATAAATAAACCAGATGCAATTAATCAGGTTCAGAAAAGTTTATCGGA
AACCGAGCATGAAGTTGATACAGAAGTTATTAACCAAGATTTTGATTTTAAATTACTTGCGCAAATGCTAAGTTCTAGTT
TTGTAGATCATCAGAGCTTGCTAGAAAATGAACATTTAATTATGGTTGAACAGACTATTTATGGGATTAATGAAAGCGAA
ATGGCTCAGTTAATCAAAGCCTCTACGAGTTATGAAGATAACAAAATTGATCCTAAAGAATTAAAGTCACGAGTTTTGAA
CGCTTATAATGGGCAGACGCCGGTTAAAGATGATGGGAATTCTCAGCCTAAAAACGAGTCAATTCCGAATAATGCGCTAG
CTAAACTTACTACAGAAGAACGCGCCATTATTAGTGCAACCTATGCTATGTCACCCAACGCATTTATTGAAAGTTTGAAA
GCACAAGCAGGGGGCTTTACAACTGCTGCAGAAAAAAGAATTATCACAGATTTAGTTGGTAAAGGGTTAATGCCAATTAG
TGTGATAAACTTTTTAGTTTACTATTTTATTGTTGATCAGGGTCGACCTACGTTAAATAAAAATTTAGTGGAAGCAATTG
CCAATGACTGGATTAAAAATAAAATTAAAACTCCAGTGGAAGCTTTAGAATTTATTCGTAAACGCCAAGAACAAAAGAAA
AATACGAACAATAATTATTCGAAAAATCGGCGTACGATTCAAAGAGAAACCTTACCTGAATGGGCTACGGAGACCGCTAA
GACTAAAGAACCCAAGCAAGTTAATCCAGAAACAACTAAAAAAATTAATGAACAACTTAAAAAACTTCGTTCTAGAGGCA
AGGAGGGATAG

Upstream 100 bases:

>100_bases
TCGTTTTGCTAGTGTTTACCGTCAATTTAAAGATATGCATGTCTTTTTAGATGAGCTGCAAGATATGATGAAAAAAGACG
AAGAAAAGGAAAAAGAGTAG

Downstream 100 bases:

>100_bases
TCATGGAAAATGTCCGAGAAATTTTGCAAGAATTAATGAGCAAAAGAAAGCTCGACCAGCGTTTTAATAACGTGATGAAA
TCAGTGTATTCTGATCCAGA

Product: replicative DNA helicase loader DnaB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MATEMSELAPHDSFIIDSQRQITDFEQRSLTTLYRPLLTSDAYSLMMVLWEMSQETKSISEQYTHTILLNWLGIDLPAIV
EARGRLEALGLLKTYQRKIDRRSLFLYALQAPADPVKFFKDDTLSALLLGVIGESEFDRLSHRLIKQHVRQDEFYDVSKK
LGDYFQLGSSVINKPDAINQVQKSLSETEHEVDTEVINQDFDFKLLAQMLSSSFVDHQSLLENEHLIMVEQTIYGINESE
MAQLIKASTSYEDNKIDPKELKSRVLNAYNGQTPVKDDGNSQPKNESIPNNALAKLTTEERAIISATYAMSPNAFIESLK
AQAGGFTTAAEKRIITDLVGKGLMPISVINFLVYYFIVDQGRPTLNKNLVEAIANDWIKNKIKTPVEALEFIRKRQEQKK
NTNNNYSKNRRTIQRETLPEWATETAKTKEPKQVNPETTKKINEQLKKLRSRGKEG

Sequences:

>Translated_456_residues
MATEMSELAPHDSFIIDSQRQITDFEQRSLTTLYRPLLTSDAYSLMMVLWEMSQETKSISEQYTHTILLNWLGIDLPAIV
EARGRLEALGLLKTYQRKIDRRSLFLYALQAPADPVKFFKDDTLSALLLGVIGESEFDRLSHRLIKQHVRQDEFYDVSKK
LGDYFQLGSSVINKPDAINQVQKSLSETEHEVDTEVINQDFDFKLLAQMLSSSFVDHQSLLENEHLIMVEQTIYGINESE
MAQLIKASTSYEDNKIDPKELKSRVLNAYNGQTPVKDDGNSQPKNESIPNNALAKLTTEERAIISATYAMSPNAFIESLK
AQAGGFTTAAEKRIITDLVGKGLMPISVINFLVYYFIVDQGRPTLNKNLVEAIANDWIKNKIKTPVEALEFIRKRQEQKK
NTNNNYSKNRRTIQRETLPEWATETAKTKEPKQVNPETTKKINEQLKKLRSRGKEG
>Mature_455_residues
ATEMSELAPHDSFIIDSQRQITDFEQRSLTTLYRPLLTSDAYSLMMVLWEMSQETKSISEQYTHTILLNWLGIDLPAIVE
ARGRLEALGLLKTYQRKIDRRSLFLYALQAPADPVKFFKDDTLSALLLGVIGESEFDRLSHRLIKQHVRQDEFYDVSKKL
GDYFQLGSSVINKPDAINQVQKSLSETEHEVDTEVINQDFDFKLLAQMLSSSFVDHQSLLENEHLIMVEQTIYGINESEM
AQLIKASTSYEDNKIDPKELKSRVLNAYNGQTPVKDDGNSQPKNESIPNNALAKLTTEERAIISATYAMSPNAFIESLKA
QAGGFTTAAEKRIITDLVGKGLMPISVINFLVYYFIVDQGRPTLNKNLVEAIANDWIKNKIKTPVEALEFIRKRQEQKKN
TNNNYSKNRRTIQRETLPEWATETAKTKEPKQVNPETTKKINEQLKKLRSRGKEG

Specific function: Probable component of primosome involved in the initiation of DNA replication. It is essential for both replication initiation and membrane attachment of the origin region of the chromosome and plasmid pUB110 [H]

COG id: COG3611

COG function: function code L; Replication initiation/membrane attachment protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006343 [H]

Pfam domain/function: PF07261 DnaB_2 [H]

EC number: NA

Molecular weight: Translated: 52031; Mature: 51900

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEMSELAPHDSFIIDSQRQITDFEQRSLTTLYRPLLTSDAYSLMMVLWEMSQETKSIS
CCCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQYTHTILLNWLGIDLPAIVEARGRLEALGLLKTYQRKIDRRSLFLYALQAPADPVKFFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHH
DDTLSALLLGVIGESEFDRLSHRLIKQHVRQDEFYDVSKKLGDYFQLGSSVINKPDAINQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VQKSLSETEHEVDTEVINQDFDFKLLAQMLSSSFVDHQSLLENEHLIMVEQTIYGINESE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHH
MAQLIKASTSYEDNKIDPKELKSRVLNAYNGQTPVKDDGNSQPKNESIPNNALAKLTTEE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RAIISATYAMSPNAFIESLKAQAGGFTTAAEKRIITDLVGKGLMPISVINFLVYYFIVDQ
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GRPTLNKNLVEAIANDWIKNKIKTPVEALEFIRKRQEQKKNTNNNYSKNRRTIQRETLPE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHH
WATETAKTKEPKQVNPETTKKINEQLKKLRSRGKEG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ATEMSELAPHDSFIIDSQRQITDFEQRSLTTLYRPLLTSDAYSLMMVLWEMSQETKSIS
CCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EQYTHTILLNWLGIDLPAIVEARGRLEALGLLKTYQRKIDRRSLFLYALQAPADPVKFFK
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHH
DDTLSALLLGVIGESEFDRLSHRLIKQHVRQDEFYDVSKKLGDYFQLGSSVINKPDAINQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VQKSLSETEHEVDTEVINQDFDFKLLAQMLSSSFVDHQSLLENEHLIMVEQTIYGINESE
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCHHH
MAQLIKASTSYEDNKIDPKELKSRVLNAYNGQTPVKDDGNSQPKNESIPNNALAKLTTEE
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
RAIISATYAMSPNAFIESLKAQAGGFTTAAEKRIITDLVGKGLMPISVINFLVYYFIVDQ
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GRPTLNKNLVEAIANDWIKNKIKTPVEALEFIRKRQEQKKNTNNNYSKNRRTIQRETLPE
CCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHH
WATETAKTKEPKQVNPETTKKINEQLKKLRSRGKEG
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3027697; 3027671; 8969504; 9387221; 9384377 [H]