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The map label for this gene is endA [H]

Identifier: 116492432

GI number: 116492432

Start: 702624

End: 703436

Strand: Direct

Name: endA [H]

Synonym: PEPE_0663

Alternate gene names: 116492432

Gene position: 702624-703436 (Clockwise)

Preceding gene: 116492431

Following gene: 116492433

Centisome position: 38.34

GC content: 35.92

Gene sequence:

>813_bases
ATGAGTAAACGAAGAAGAAAAAATAAGCAGTCTACTAGTATTTGGCTTGTTTTAGTAATTGCCATTTTAGGAACTTTTTT
CAAAGGAAATGATATTCATCAGTTTGTTTCTAATCTGACTAGTCAGGGGATCCCACAAACGACTAAATCGGTTAAAAGTG
TCGAGGATACTCAATTATCTCAACAAACTTTTAAATCCGGTGGAACAGCTTATATTTACGTTAACAATAATAAAAGTACC
CTTAATCCCGCAGATTGGAAGAAAAACCAAATTATTTACGGTAATTTAGATCGTTTGAATCGGACGACACAAGCAACGGG
ATATCTAAGTCGTGTAAACTTAGTTAAGTCGAATACCAGAACCAGTCAGACTTGGAATCCAACTGGTTGGCATCAAAAAC
GTTTAACCATTAACGGTAAAACAACTGAAATTCAGAACCGAGGCCATTTACTAGCATATTCAATTACTGGTAAATTTGAT
CAAAATGGGAATTTCAATGCTCATGAATTAGGATCATTAGATAATCCCAAAAATCTAGCTACTCAAACAGCATACAGTAA
TCAGCAGACTATGCAAATTTTTGAGGAACGGGTACGGGATGCATTAGCTAAGAATAAAAAGGTTATTTACCGCGTTTCGA
CTGTTTTTAAAGGAAATGGATTAATGCCAACTGGCTACCATGCGGAAGCTATTGCAACAGATGGATCGTTAAATTTTAAC
GTTTTTGTTTGGAATGTACAACCAGGGGTTCAATTTGATTATGCAACCGGACGATCACGAGTGGATCGTAGTATGACAGT
AAGAGCAAATTAA

Upstream 100 bases:

>100_bases
GATCGAAATGGCTTTGAAACTAAGAAAGTAAAAATAAAAGTTGGGATTAAACTAAACCAGTTTGTCCCAACTTTTTACTA
TGATTAACTTGGAGGAAGAG

Downstream 100 bases:

>100_bases
AGATTAAACGATTAATCGATATGATTAATCGTTTTTTTATTACGTTAAACTTTTTAAATTGGAGTGGAAGATATATACTA
AAAACATAAATCTATATGAT

Product: DNA-entry nuclease

Products: NA

Alternate protein names: Competence-specific nuclease [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MSKRRRKNKQSTSIWLVLVIAILGTFFKGNDIHQFVSNLTSQGIPQTTKSVKSVEDTQLSQQTFKSGGTAYIYVNNNKST
LNPADWKKNQIIYGNLDRLNRTTQATGYLSRVNLVKSNTRTSQTWNPTGWHQKRLTINGKTTEIQNRGHLLAYSITGKFD
QNGNFNAHELGSLDNPKNLATQTAYSNQQTMQIFEERVRDALAKNKKVIYRVSTVFKGNGLMPTGYHAEAIATDGSLNFN
VFVWNVQPGVQFDYATGRSRVDRSMTVRAN

Sequences:

>Translated_270_residues
MSKRRRKNKQSTSIWLVLVIAILGTFFKGNDIHQFVSNLTSQGIPQTTKSVKSVEDTQLSQQTFKSGGTAYIYVNNNKST
LNPADWKKNQIIYGNLDRLNRTTQATGYLSRVNLVKSNTRTSQTWNPTGWHQKRLTINGKTTEIQNRGHLLAYSITGKFD
QNGNFNAHELGSLDNPKNLATQTAYSNQQTMQIFEERVRDALAKNKKVIYRVSTVFKGNGLMPTGYHAEAIATDGSLNFN
VFVWNVQPGVQFDYATGRSRVDRSMTVRAN
>Mature_269_residues
SKRRRKNKQSTSIWLVLVIAILGTFFKGNDIHQFVSNLTSQGIPQTTKSVKSVEDTQLSQQTFKSGGTAYIYVNNNKSTL
NPADWKKNQIIYGNLDRLNRTTQATGYLSRVNLVKSNTRTSQTWNPTGWHQKRLTINGKTTEIQNRGHLLAYSITGKFDQ
NGNFNAHELGSLDNPKNLATQTAYSNQQTMQIFEERVRDALAKNKKVIYRVSTVFKGNGLMPTGYHAEAIATDGSLNFNV
FVWNVQPGVQFDYATGRSRVDRSMTVRAN

Specific function: By degrading DNA that enters the cell, plays a role in the competence of cells to be transformed [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA/RNA non-specific endonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018524
- InterPro:   IPR001604 [H]

Pfam domain/function: PF01223 Endonuclease_NS [H]

EC number: 3.1.30.-

Molecular weight: Translated: 30447; Mature: 30315

Theoretical pI: Translated: 10.85; Mature: 10.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRRRKNKQSTSIWLVLVIAILGTFFKGNDIHQFVSNLTSQGIPQTTKSVKSVEDTQLS
CCCHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QQTFKSGGTAYIYVNNNKSTLNPADWKKNQIIYGNLDRLNRTTQATGYLSRVNLVKSNTR
HHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHEEEECCCC
TSQTWNPTGWHQKRLTINGKTTEIQNRGHLLAYSITGKFDQNGNFNAHELGSLDNPKNLA
CCCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCHHHH
TQTAYSNQQTMQIFEERVRDALAKNKKVIYRVSTVFKGNGLMPTGYHAEAIATDGSLNFN
HHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCCEEEE
VFVWNVQPGVQFDYATGRSRVDRSMTVRAN
EEEEEECCCCEEEECCCCHHCCCEEEEECC
>Mature Secondary Structure 
SKRRRKNKQSTSIWLVLVIAILGTFFKGNDIHQFVSNLTSQGIPQTTKSVKSVEDTQLS
CCHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
QQTFKSGGTAYIYVNNNKSTLNPADWKKNQIIYGNLDRLNRTTQATGYLSRVNLVKSNTR
HHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHEEEECCCC
TSQTWNPTGWHQKRLTINGKTTEIQNRGHLLAYSITGKFDQNGNFNAHELGSLDNPKNLA
CCCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEEECCCCCCCCCCHHHCCCCCCCHHHH
TQTAYSNQQTMQIFEERVRDALAKNKKVIYRVSTVFKGNGLMPTGYHAEAIATDGSLNFN
HHHHCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCEEEECCCCEEEE
VFVWNVQPGVQFDYATGRSRVDRSMTVRAN
EEEEEECCCCEEEECCCCHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2359120; 11463916 [H]