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The map label for this gene is prfC

Identifier: 116492397

GI number: 116492397

Start: 657038

End: 658606

Strand: Direct

Name: prfC

Synonym: PEPE_0603

Alternate gene names: 116492397

Gene position: 657038-658606 (Clockwise)

Preceding gene: 116492396

Following gene: 116492398

Centisome position: 35.86

GC content: 41.81

Gene sequence:

>1569_bases
ATGAATCAACAAGATTTAAAAAATGCGGTGGAAAAACGCCGGACGTTTGCCATTATTTCCCATCCGGATGCTGGTAAGAC
GACGATTACAGAACAACTATTGCTCTTCGGGGGCGTTGTGCGCGAAGCCGGAACGGTTAAAGCCCGTAAATCAGGCAACT
ATGCTAAGTCGGACTGGATGGAAATTGAAAAGAAACGTGGAATTTCGGTTACTAGTTCGGTTATGCAATTTGATTACGCT
GGTAAACGAATTAACATCTTGGATACTCCTGGACATGAGGATTTCTCAGAAGATACTTATCGAACCTTGATGGCCGTGGA
TTCAGCTGTCATGGTAATTGACTCCGCCAAAGGGATTGAGCCCCAAACTAAAAAGCTTTTCCAAATTTGTAAAATGCGCG
GTATTCCAATTTTCACTTTCATGAACAAGTTGGATCGGGATGGACGCGAACCCCTAGACTTGGTTAATGAATTGGAAGAA
GTCTTAGGCGTAGAAGCTTATCCGATGAACTGGCCAATTGGAATGGGTAAGGGACTAGCCGGATTGTACGATATTTACAA
CAACCGCATTGAACTACATACTGATGAACCAGAATTTTTGGAACTTAATGCTGACGGCGAACTCAAAGAAAGCAATCCGA
TCGAAGAGGAAAGTGTTTTCCAAGACGCCATGATGGAAGTTTCTTTAATGAAAGAAGCTGGCAATCAATTTGATGAAGCC
AAGATTGCTACTGGGGAATTGACTCCCGTCTTCTTTGGTTCGGCTTTGACTAACTTTGGGGTTAAGACTTTCTTGGATGC
TTACCTCCAATATGCTCCTGCACCAACTTCACATAAAGATGAAGCCGGTGAAGAAATCGAACCAACTGAAGAAGAATTTT
CAGGATTTGTTTTCAAGATTCAAGCTAATATGAATCCTAATCACCGTGACCGGATTGCCTTTTTGCGCATTTGCTCTGGA
GAATTTGATCGTGGGATGGACGTCACTTTGAACCGGACGGGTAAAACTTTACGCCTTTCTAATTCCACTCAATTCATGGC
AGACACACGTGAAAACGTGGAAACAGCGGTTGCGGGAGATATCATCGGACTTTATGATACGGGTAACTTCCAAATTGGGG
ATACTATTTACGCTGGCAAACATGCCATTCAATTTGAAAAATTACCACAATTTACACCGGAACTATTTATGCAAGTTTCC
GCTAAAAACGTGATGAAGCAAAAGTCCTTCCATAAGGGAATCAAGCAATTGGTTCAAGAAGGAGCTGTCCAGCTTTATAC
TAACTACACTACTGGAGATTATATTCTCGGTGCCGTAGGTCAACTTCAATTTGAAGTTTTCCAATTCCGGATGCAAAATG
AATACAATTCCGAAGTAGTTATGCAACCTATGGGTAAAAAGACTGCGCGCTGGATTGACCCAGAACAACTCGATGAAAAA
ATGTCGTCTTCACGTAACTTATTGGTTAAAGACCGTTATGGACAACCATTGTTCTTATTTGAAAACCAATTTGCAGAACG
TTGGTTTGCTGGTAAATACCCAGATGTTAAACTAACGGCCAAACTCTAA

Upstream 100 bases:

>100_bases
GATCTTGGACTAGGCGATTACAAAAAAGATTAGGACTACAAACTAAGATTGGAATGTCTAGACGATTCCAATCTTATTTT
TTAGAGAGAAAGGAACTACT

Downstream 100 bases:

>100_bases
AAACTAAATCTTTCTAAATTAACACCTCAAATGGGGTTAATTTTTTAGTTTTTAATATATAATGGTTAAGTTGTATCAAG
AAATATCACGTGGAGGAACG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MNQQDLKNAVEKRRTFAIISHPDAGKTTITEQLLLFGGVVREAGTVKARKSGNYAKSDWMEIEKKRGISVTSSVMQFDYA
GKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIEPQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLVNELEE
VLGVEAYPMNWPIGMGKGLAGLYDIYNNRIELHTDEPEFLELNADGELKESNPIEEESVFQDAMMEVSLMKEAGNQFDEA
KIATGELTPVFFGSALTNFGVKTFLDAYLQYAPAPTSHKDEAGEEIEPTEEEFSGFVFKIQANMNPNHRDRIAFLRICSG
EFDRGMDVTLNRTGKTLRLSNSTQFMADTRENVETAVAGDIIGLYDTGNFQIGDTIYAGKHAIQFEKLPQFTPELFMQVS
AKNVMKQKSFHKGIKQLVQEGAVQLYTNYTTGDYILGAVGQLQFEVFQFRMQNEYNSEVVMQPMGKKTARWIDPEQLDEK
MSSSRNLLVKDRYGQPLFLFENQFAERWFAGKYPDVKLTAKL

Sequences:

>Translated_522_residues
MNQQDLKNAVEKRRTFAIISHPDAGKTTITEQLLLFGGVVREAGTVKARKSGNYAKSDWMEIEKKRGISVTSSVMQFDYA
GKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIEPQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLVNELEE
VLGVEAYPMNWPIGMGKGLAGLYDIYNNRIELHTDEPEFLELNADGELKESNPIEEESVFQDAMMEVSLMKEAGNQFDEA
KIATGELTPVFFGSALTNFGVKTFLDAYLQYAPAPTSHKDEAGEEIEPTEEEFSGFVFKIQANMNPNHRDRIAFLRICSG
EFDRGMDVTLNRTGKTLRLSNSTQFMADTRENVETAVAGDIIGLYDTGNFQIGDTIYAGKHAIQFEKLPQFTPELFMQVS
AKNVMKQKSFHKGIKQLVQEGAVQLYTNYTTGDYILGAVGQLQFEVFQFRMQNEYNSEVVMQPMGKKTARWIDPEQLDEK
MSSSRNLLVKDRYGQPLFLFENQFAERWFAGKYPDVKLTAKL
>Mature_522_residues
MNQQDLKNAVEKRRTFAIISHPDAGKTTITEQLLLFGGVVREAGTVKARKSGNYAKSDWMEIEKKRGISVTSSVMQFDYA
GKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIEPQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLVNELEE
VLGVEAYPMNWPIGMGKGLAGLYDIYNNRIELHTDEPEFLELNADGELKESNPIEEESVFQDAMMEVSLMKEAGNQFDEA
KIATGELTPVFFGSALTNFGVKTFLDAYLQYAPAPTSHKDEAGEEIEPTEEEFSGFVFKIQANMNPNHRDRIAFLRICSG
EFDRGMDVTLNRTGKTLRLSNSTQFMADTRENVETAVAGDIIGLYDTGNFQIGDTIYAGKHAIQFEKLPQFTPELFMQVS
AKNVMKQKSFHKGIKQLVQEGAVQLYTNYTTGDYILGAVGQLQFEVFQFRMQNEYNSEVVMQPMGKKTARWIDPEQLDEK
MSSSRNLLVKDRYGQPLFLFENQFAERWFAGKYPDVKLTAKL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=486, Percent_Identity=28.6008230452675, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI19923640, Length=548, Percent_Identity=26.4598540145985, Blast_Score=163, Evalue=4e-40,
Organism=Homo sapiens, GI25306283, Length=395, Percent_Identity=29.873417721519, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25306287, Length=521, Percent_Identity=26.1036468330134, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=30.8176100628931, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=30.6569343065693, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=29.5302013422819, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=516, Percent_Identity=46.7054263565892, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789738, Length=485, Percent_Identity=29.2783505154639, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI48994988, Length=176, Percent_Identity=33.5227272727273, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=28.9308176100629, Blast_Score=90, Evalue=5e-19,
Organism=Escherichia coli, GI1789737, Length=132, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790412, Length=132, Percent_Identity=30.3030303030303, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17533571, Length=491, Percent_Identity=26.8839103869654, Blast_Score=185, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17556745, Length=180, Percent_Identity=36.6666666666667, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=138, Percent_Identity=31.1594202898551, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=546, Percent_Identity=23.992673992674, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=31.6176470588235, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=146, Percent_Identity=30.1369863013699, Blast_Score=74, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17556456, Length=172, Percent_Identity=27.906976744186, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=27.1398747390397, Blast_Score=173, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=26.0194174757282, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6323320, Length=171, Percent_Identity=29.2397660818713, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=214, Percent_Identity=28.0373831775701, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=214, Percent_Identity=28.0373831775701, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=171, Percent_Identity=30.4093567251462, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=491, Percent_Identity=26.4765784114053, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI221458488, Length=515, Percent_Identity=27.1844660194175, Blast_Score=165, Evalue=9e-41,
Organism=Drosophila melanogaster, GI78706572, Length=160, Percent_Identity=31.25, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24585711, Length=538, Percent_Identity=21.9330855018587, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585713, Length=538, Percent_Identity=21.9330855018587, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24585709, Length=538, Percent_Identity=21.9330855018587, Blast_Score=76, Evalue=5e-14,
Organism=Drosophila melanogaster, GI281363316, Length=169, Percent_Identity=28.9940828402367, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864358, Length=169, Percent_Identity=28.9940828402367, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI28574573, Length=171, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_PEDPA (Q03GI2)

Other databases:

- EMBL:   CP000422
- RefSeq:   YP_804132.1
- ProteinModelPortal:   Q03GI2
- SMR:   Q03GI2
- STRING:   Q03GI2
- GeneID:   4418224
- GenomeReviews:   CP000422_GR
- KEGG:   ppe:PEPE_0603
- NMPDR:   fig|278197.10.peg.555
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- PhylomeDB:   Q03GI2
- ProtClustDB:   PRK00741
- BioCyc:   PPEN278197:PEPE_0603-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59092; Mature: 59092

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQQDLKNAVEKRRTFAIISHPDAGKTTITEQLLLFGGVVREAGTVKARKSGNYAKSDWM
CCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHH
EIEKKRGISVTSSVMQFDYAGKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIE
HHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
PQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLVNELEEVLGVEAYPMNWPIGMGKGLA
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
GLYDIYNNRIELHTDEPEFLELNADGELKESNPIEEESVFQDAMMEVSLMKEAGNQFDEA
HHHHHHCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
KIATGELTPVFFGSALTNFGVKTFLDAYLQYAPAPTSHKDEAGEEIEPTEEEFSGFVFKI
EECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCEEEEE
QANMNPNHRDRIAFLRICSGEFDRGMDVTLNRTGKTLRLSNSTQFMADTRENVETAVAGD
EECCCCCCHHHEEEEEEECCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCC
IIGLYDTGNFQIGDTIYAGKHAIQFEKLPQFTPELFMQVSAKNVMKQKSFHKGIKQLVQE
EEEEEECCCEEECCEEECCCHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAVQLYTNYTTGDYILGAVGQLQFEVFQFRMQNEYNSEVVMQPMGKKTARWIDPEQLDEK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHCCCCHHHHHHH
MSSSRNLLVKDRYGQPLFLFENQFAERWFAGKYPDVKLTAKL
HHCCCCEEEEECCCCEEEEECHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure
MNQQDLKNAVEKRRTFAIISHPDAGKTTITEQLLLFGGVVREAGTVKARKSGNYAKSDWM
CCHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHH
EIEKKRGISVTSSVMQFDYAGKRINILDTPGHEDFSEDTYRTLMAVDSAVMVIDSAKGIE
HHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCCC
PQTKKLFQICKMRGIPIFTFMNKLDRDGREPLDLVNELEEVLGVEAYPMNWPIGMGKGLA
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHH
GLYDIYNNRIELHTDEPEFLELNADGELKESNPIEEESVFQDAMMEVSLMKEAGNQFDEA
HHHHHHCCEEEEECCCCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
KIATGELTPVFFGSALTNFGVKTFLDAYLQYAPAPTSHKDEAGEEIEPTEEEFSGFVFKI
EECCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHCCCEEEEE
QANMNPNHRDRIAFLRICSGEFDRGMDVTLNRTGKTLRLSNSTQFMADTRENVETAVAGD
EECCCCCCHHHEEEEEEECCCCCCCCEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCC
IIGLYDTGNFQIGDTIYAGKHAIQFEKLPQFTPELFMQVSAKNVMKQKSFHKGIKQLVQE
EEEEEECCCEEECCEEECCCHHEEHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAVQLYTNYTTGDYILGAVGQLQFEVFQFRMQNEYNSEVVMQPMGKKTARWIDPEQLDEK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHCCCCHHHHHHH
MSSSRNLLVKDRYGQPLFLFENQFAERWFAGKYPDVKLTAKL
HHCCCCEEEEECCCCEEEEECHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA