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The map label for this gene is sau3AIM [H]

Identifier: 116492328

GI number: 116492328

Start: 587203

End: 588489

Strand: Direct

Name: sau3AIM [H]

Synonym: PEPE_0532

Alternate gene names: 116492328

Gene position: 587203-588489 (Clockwise)

Preceding gene: 116492326

Following gene: 116492329

Centisome position: 32.05

GC content: 33.57

Gene sequence:

>1287_bases
ATGACAAAAGAATTGAAAGTTTTAGAATTGTTCGCAGGAGTTGGAGGATTTCGTGTAGGGTTAGAGCATGCAAATGCTAG
CTTATACAAGACGTTATGGTCAAATCAATGGGAACCTGGAAAAAAATCACAGGATGCTTTTGAAGTTTATAATTATCATT
TTCCGAATTCGGAAAATATAAACGAAAATATCTCTGAAATATCTAACGAAAAATTTAAAACGATGAATGCAGACTTGATT
GTTGGTGGCTTTCCTTGTCAAGATTATTCGGTTGCACGTAGTAAGAAGCATGAAATGGGTATTGAAGGAAAAAAAGGAGT
TTTATTCTGGGATATTATTAGAGCGGTAGAGAATATACATCCTAAATACTTAATTTTAGAAAATGTGGACCGCCTTTTAA
AGTCACCTTCTACCCAACGTGGTCGTGATTTTGCCATTATGCTGGCTTCGTTTAATAAGTTAGGTTATTCGGTAGAATGG
AGAGTTATTAATGCTGCTGAGTATGGATTTGGGCAAAGACGCAGGCGAGTGTATTTTTTTGTTTACCGAAATGATACGCC
ATTTGCTAAGAAAATGGATGAATTATTTGAAACGGATATAGAAAGTGTATTGGTTGACGAAACTAAATATGATGAATATA
TCTTCTCGAAAGGTTTATTTGCGCGTCAATTTCCAATAAAACAAGAGGTTTATAAAAAGAGACATGATTATTACGAATTA
TCAGATGATATTGTAAATATTTCAGATAATTTTAGTGGTAAAGTATGGAATACAGGTATAATGCGTCATGGTCGGTATTT
TACAATTGATACGAAACCTTTGGGTAATGAAGAACCAATTCCACTAAAAGATACCTTACAAAGGGATACTGAAATTGATG
AGAAATTCTTTATTACTGATAAAAATAAAATAGAGAAATTTGCATATCTCAGAGGTCCTAAAAAAATTCAAAGAGTTAGC
GCTAGCGGTCATGAATACACTTACTCTGAAGGTGGAATGTCACCTTATGAGAATATTGATTTACCTAGTCGAACTATGCT
AACTTCAGAAGGAACAGTTAATCGATCAACCCACTTCATTAAAACAAAAGACAATCGTTATCGTTTGATAACTCCAATTG
AGGCTGAAAGGCTGCAGAATTTTGATGATGATTGGACTAAAATTAAGAAATTAGCAGATGGTTCCACAACACAGGTTTCG
GATAGAATGCGAATGTTTTTTATGGGGAATGCATTGGTAACTGGTGTTATTAAAAGAATTGGAATTGAATTAGAAAAAAT
AATCTAG

Upstream 100 bases:

>100_bases
CTCCAGCTAGTGCTTTTTAATTTATTATATCACTTGATAATGTTGATCAATTGCGTATTTTAATGTATAATAACTAATAC
GAAAGGATGTTCGCTATATT

Downstream 100 bases:

>100_bases
AGTTTGATAAATTTCTGTATAAAAAAGTAAGAAGTTAATTAAAAAATAACTTTTTACTTTTTTTGCTATTTCTAGTTAAA
TAGGTATATTAAATGGATAA

Product: site-specific DNA methylase

Products: NA

Alternate protein names: M.Sau3AI; Cytosine-specific methyltransferase Sau3AI [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MTKELKVLELFAGVGGFRVGLEHANASLYKTLWSNQWEPGKKSQDAFEVYNYHFPNSENINENISEISNEKFKTMNADLI
VGGFPCQDYSVARSKKHEMGIEGKKGVLFWDIIRAVENIHPKYLILENVDRLLKSPSTQRGRDFAIMLASFNKLGYSVEW
RVINAAEYGFGQRRRRVYFFVYRNDTPFAKKMDELFETDIESVLVDETKYDEYIFSKGLFARQFPIKQEVYKKRHDYYEL
SDDIVNISDNFSGKVWNTGIMRHGRYFTIDTKPLGNEEPIPLKDTLQRDTEIDEKFFITDKNKIEKFAYLRGPKKIQRVS
ASGHEYTYSEGGMSPYENIDLPSRTMLTSEGTVNRSTHFIKTKDNRYRLITPIEAERLQNFDDDWTKIKKLADGSTTQVS
DRMRMFFMGNALVTGVIKRIGIELEKII

Sequences:

>Translated_428_residues
MTKELKVLELFAGVGGFRVGLEHANASLYKTLWSNQWEPGKKSQDAFEVYNYHFPNSENINENISEISNEKFKTMNADLI
VGGFPCQDYSVARSKKHEMGIEGKKGVLFWDIIRAVENIHPKYLILENVDRLLKSPSTQRGRDFAIMLASFNKLGYSVEW
RVINAAEYGFGQRRRRVYFFVYRNDTPFAKKMDELFETDIESVLVDETKYDEYIFSKGLFARQFPIKQEVYKKRHDYYEL
SDDIVNISDNFSGKVWNTGIMRHGRYFTIDTKPLGNEEPIPLKDTLQRDTEIDEKFFITDKNKIEKFAYLRGPKKIQRVS
ASGHEYTYSEGGMSPYENIDLPSRTMLTSEGTVNRSTHFIKTKDNRYRLITPIEAERLQNFDDDWTKIKKLADGSTTQVS
DRMRMFFMGNALVTGVIKRIGIELEKII
>Mature_427_residues
TKELKVLELFAGVGGFRVGLEHANASLYKTLWSNQWEPGKKSQDAFEVYNYHFPNSENINENISEISNEKFKTMNADLIV
GGFPCQDYSVARSKKHEMGIEGKKGVLFWDIIRAVENIHPKYLILENVDRLLKSPSTQRGRDFAIMLASFNKLGYSVEWR
VINAAEYGFGQRRRRVYFFVYRNDTPFAKKMDELFETDIESVLVDETKYDEYIFSKGLFARQFPIKQEVYKKRHDYYELS
DDIVNISDNFSGKVWNTGIMRHGRYFTIDTKPLGNEEPIPLKDTLQRDTEIDEKFFITDKNKIEKFAYLRGPKKIQRVSA
SGHEYTYSEGGMSPYENIDLPSRTMLTSEGTVNRSTHFIKTKDNRYRLITPIEAERLQNFDDDWTKIKKLADGSTTQVSD
RMRMFFMGNALVTGVIKRIGIELEKII

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on C-4 on both strands and protects the DNA from cleavage by the Sau3AI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788271, Length=210, Percent_Identity=25.7142857142857, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 49826; Mature: 49695

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKELKVLELFAGVGGFRVGLEHANASLYKTLWSNQWEPGKKSQDAFEVYNYHFPNSENI
CCCCHHHHHHHHCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCHHHHEEEECCCCCCC
NENISEISNEKFKTMNADLIVGGFPCQDYSVARSKKHEMGIEGKKGVLFWDIIRAVENIH
CHHHHHHCCHHHEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
PKYLILENVDRLLKSPSTQRGRDFAIMLASFNKLGYSVEWRVINAAEYGFGQRRRRVYFF
CCEEEHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCEEEEEEEEHHHCCCCCCCCEEEEE
VYRNDTPFAKKMDELFETDIESVLVDETKYDEYIFSKGLFARQFPIKQEVYKKRHDYYEL
EEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCCCHHHHHHHHCCCEEC
SDDIVNISDNFSGKVWNTGIMRHGRYFTIDTKPLGNEEPIPLKDTLQRDTEIDEKFFITD
CCCEEECCCCCCCCEEECHHEECCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEEC
KNKIEKFAYLRGPKKIQRVSASGHEYTYSEGGMSPYENIDLPSRTMLTSEGTVNRSTHFI
HHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCEEEE
KTKDNRYRLITPIEAERLQNFDDDWTKIKKLADGSTTQVSDRMRMFFMGNALVTGVIKRI
EECCCCEEEEECCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHEEEHCCHHHHHHHHHHH
GIELEKII
CCHHHHCC
>Mature Secondary Structure 
TKELKVLELFAGVGGFRVGLEHANASLYKTLWSNQWEPGKKSQDAFEVYNYHFPNSENI
CCCHHHHHHHHCCCCEEEEHHHCCHHHHHHHHCCCCCCCCCCCCHHHHEEEECCCCCCC
NENISEISNEKFKTMNADLIVGGFPCQDYSVARSKKHEMGIEGKKGVLFWDIIRAVENIH
CHHHHHHCCHHHEECCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
PKYLILENVDRLLKSPSTQRGRDFAIMLASFNKLGYSVEWRVINAAEYGFGQRRRRVYFF
CCEEEHHHHHHHHCCCCCCCCCEEEEEEEHHHHCCCEEEEEEEEHHHCCCCCCCCEEEEE
VYRNDTPFAKKMDELFETDIESVLVDETKYDEYIFSKGLFARQFPIKQEVYKKRHDYYEL
EEECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHCCCCHHHHHHHHCCCEEC
SDDIVNISDNFSGKVWNTGIMRHGRYFTIDTKPLGNEEPIPLKDTLQRDTEIDEKFFITD
CCCEEECCCCCCCCEEECHHEECCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCEEEEEC
KNKIEKFAYLRGPKKIQRVSASGHEYTYSEGGMSPYENIDLPSRTMLTSEGTVNRSTHFI
HHHHHHHHHHCCHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCEEEE
KTKDNRYRLITPIEAERLQNFDDDWTKIKKLADGSTTQVSDRMRMFFMGNALVTGVIKRI
EECCCCEEEEECCCHHHHCCCCHHHHHHHHHCCCCCHHHHHHHEEEHCCHHHHHHHHHHH
GIELEKII
CCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2227451 [H]