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The map label for this gene is insF1 [H]

Identifier: 116492322

GI number: 116492322

Start: 579439

End: 580029

Strand: Direct

Name: insF1 [H]

Synonym: PEPE_0524

Alternate gene names: 116492322

Gene position: 579439-580029 (Clockwise)

Preceding gene: 116492321

Following gene: 116492323

Centisome position: 31.62

GC content: 43.15

Gene sequence:

>591_bases
ATGAAGGAACTGGATATCCACTCCGTTATCGTCAATAAGTGGAAAGCGGCTTCGGCTTCCAAAACCAAGGTTGAACAGCG
TCCCAACTTGCTTAAGCAGGATTTCTCGATCACTGGTTTAAATCAAAAATGGACCGCTGATATGACCTATATTCAAACGA
AGCGTAATGGCTGGTGTTACTTATCAACCATCATGGACCTGCACTCACGACGGATTATCGGCTATTCGTTCTCAAAACAG
ATGGATGCTGATTTAGTCTTAAAGACCCTTGAAAGCGCGGTTAAAAATCGAACCATTACTGGGGACCTGATTATCCATAC
GGATTTAGGATCACAGTATACCAGCGATGATTACAATCAACGTTTAACTGAACTACATATCCGCCACTCATATAGCCGTA
AGGGTTGTCCGTATGATAATGCGCCAATGGAATCCTTTCACGCTTCCCTCAAAAAAGAATGTGTTTATCCAGTGCCGGTC
TTTGAAGATTATGAAACTGCCGCTGCCGTCCTTTTTGAATATGTGCATGCTTTCTACAATAGGAAGAGAATTCATAGTTC
ACTGGGCTACCAGACCCCCCTTACAAGTTGA

Upstream 100 bases:

>100_bases
ATTTAAGCAGCGATACGGTGTTATGAAGATCCACCATGAATTGAATCTGGAACTTCAACCACTGCAGCTTCGGTGCAGTC
CACGACGGATTTCCCGGCTC

Downstream 100 bases:

>100_bases
AATTGCAACACTTACGAGCCAAATGGCTGCCTGATTTAATGCTTTCCAGGGTTCAAATAAGTTATTAATCGCGATTAATG
ATTTATTTGAGCTGTGGAAG

Product: transposase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 196; Mature: 196

Protein sequence:

>196_residues
MKELDIHSVIVNKWKAASASKTKVEQRPNLLKQDFSITGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKQ
MDADLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPV
FEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLTS

Sequences:

>Translated_196_residues
MKELDIHSVIVNKWKAASASKTKVEQRPNLLKQDFSITGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKQ
MDADLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPV
FEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLTS
>Mature_196_residues
MKELDIHSVIVNKWKAASASKTKVEQRPNLLKQDFSITGLNQKWTADMTYIQTKRNGWCYLSTIMDLHSRRIIGYSFSKQ
MDADLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQRLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPV
FEDYETAAAVLFEYVHAFYNRKRIHSSLGYQTPLTS

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=155, Percent_Identity=35.4838709677419, Blast_Score=101, Evalue=4e-22,
Organism=Escherichia coli, GI1788405, Length=165, Percent_Identity=45.4545454545455, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1787263, Length=165, Percent_Identity=45.4545454545455, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1786753, Length=165, Percent_Identity=45.4545454545455, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1786570, Length=165, Percent_Identity=45.4545454545455, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1786490, Length=165, Percent_Identity=45.4545454545455, Blast_Score=143, Evalue=9e-36,
Organism=Escherichia coli, GI1789981, Length=168, Percent_Identity=35.7142857142857, Blast_Score=94, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 22638; Mature: 22638

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELDIHSVIVNKWKAASASKTKVEQRPNLLKQDFSITGLNQKWTADMTYIQTKRNGWCY
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCEE
LSTIMDLHSRRIIGYSFSKQMDADLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQ
HHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
RLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYN
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RKRIHSSLGYQTPLTS
HHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKELDIHSVIVNKWKAASASKTKVEQRPNLLKQDFSITGLNQKWTADMTYIQTKRNGWCY
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCEEEEECCCCCEE
LSTIMDLHSRRIIGYSFSKQMDADLVLKTLESAVKNRTITGDLIIHTDLGSQYTSDDYNQ
HHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHH
RLTELHIRHSYSRKGCPYDNAPMESFHASLKKECVYPVPVFEDYETAAAVLFEYVHAFYN
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
RKRIHSSLGYQTPLTS
HHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]