Click here to switch to the map view.

The map label for this gene is 116492026

Identifier: 116492026

GI number: 116492026

Start: 258293

End: 259213

Strand: Reverse

Name: 116492026

Synonym: PEPE_0218

Alternate gene names: NA

Gene position: 259213-258293 (Counterclockwise)

Preceding gene: 116492027

Following gene: 116492024

Centisome position: 14.15

GC content: 42.89

Gene sequence:

>921_bases
ATGACACGAAAAATTGGAATCATTGGTATGGGTCACGTTGGTCAAGCAGTCGCTCAACAAATTATCACAAACGGCTACGC
TGACGACCTAGTTTTAATAGATAAAAATGAAGCAAAGGTTGGTGCTGATGCACTTGATTTTGAAGATGCAATGCCCAATT
TAACCAGTCACACAAACATTATCGTAAATGATTATCGTGCCCTCAAAGATGCAGACGTAATTATTTCTGCTTTGGGTAAG
ATTGCTTTAACTGATAACCCTGATAATGACCGTTTTGCTGAACTTCCGCACAACCGTGAACAAGTTCAGGCGGTCGCTGA
AGCCATTAAAGCATCTGGATTTAATGGTATTTTAATTCCAATTACTAATCCAGTAGATGTAATTACCGCACTTTACCAAG
AAGCCACCGGCTTACCTAAAGCGCATGTTATTGGAACTGGTACTCTACTTGATTCAGCCCGCATGAAGCGCGCCGTCGGC
AAAGGTTTAGGCGTTGACCCTCGCTCGGTATCTGGATATAACCTCGGTGAACATGGTAATTCACAATTTACCGCTTGGTC
CACGGTTACTGTTCTTGACCAACCAATCAAAAAGTTAGCTCAAGCTCGGAATTTAGACTTAGATCAAATCAACGAAGACG
CTCGGATGGGCGGTCGCTATGTAATGGTTGGTAAACACTATACTAACTATGGGGTTGCTGCCGCTGCCGTTCGTTTGGCT
AATGTAGTTTTAAATGATGCTCATGAAGTTCTTCCCGTTTCCAATTATCGTGAAGAATATGGTACCTATCTTTCATATCC
GGCCGTGGTCGGACGTGACGGTATTTTAGAACAGCTCCAACTCGAACTTACCGATGAAGAACGCTACAAGTTACGTCGTT
CAGCCGACTTCATCGCCGAAAAAATCAAAGAATCTAAGTAA

Upstream 100 bases:

>100_bases
AGGCAGCCTTGAAACAAATTAATCCTTAATTTCTTCAAAGCACTTTAACGTTAGCTTTTTTCAAAAATGTGCCTTAAAAT
ATGGGTAGAGGTGATGGATA

Downstream 100 bases:

>100_bases
AAAATAAAAAACTTCAATCTTTGCTTAATCGCGATTGAAGTTTTTTATTTTCGTCTTACTTTTTGTACTTATTTTCACAT
GAAGCAAAGATGCTTAATCG

Product: lactate dehydrogenase

Products: NA

Alternate protein names: L-HicDH [H]

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MTRKIGIIGMGHVGQAVAQQIITNGYADDLVLIDKNEAKVGADALDFEDAMPNLTSHTNIIVNDYRALKDADVIISALGK
IALTDNPDNDRFAELPHNREQVQAVAEAIKASGFNGILIPITNPVDVITALYQEATGLPKAHVIGTGTLLDSARMKRAVG
KGLGVDPRSVSGYNLGEHGNSQFTAWSTVTVLDQPIKKLAQARNLDLDQINEDARMGGRYVMVGKHYTNYGVAAAAVRLA
NVVLNDAHEVLPVSNYREEYGTYLSYPAVVGRDGILEQLQLELTDEERYKLRRSADFIAEKIKESK

Sequences:

>Translated_306_residues
MTRKIGIIGMGHVGQAVAQQIITNGYADDLVLIDKNEAKVGADALDFEDAMPNLTSHTNIIVNDYRALKDADVIISALGK
IALTDNPDNDRFAELPHNREQVQAVAEAIKASGFNGILIPITNPVDVITALYQEATGLPKAHVIGTGTLLDSARMKRAVG
KGLGVDPRSVSGYNLGEHGNSQFTAWSTVTVLDQPIKKLAQARNLDLDQINEDARMGGRYVMVGKHYTNYGVAAAAVRLA
NVVLNDAHEVLPVSNYREEYGTYLSYPAVVGRDGILEQLQLELTDEERYKLRRSADFIAEKIKESK
>Mature_305_residues
TRKIGIIGMGHVGQAVAQQIITNGYADDLVLIDKNEAKVGADALDFEDAMPNLTSHTNIIVNDYRALKDADVIISALGKI
ALTDNPDNDRFAELPHNREQVQAVAEAIKASGFNGILIPITNPVDVITALYQEATGLPKAHVIGTGTLLDSARMKRAVGK
GLGVDPRSVSGYNLGEHGNSQFTAWSTVTVLDQPIKKLAQARNLDLDQINEDARMGGRYVMVGKHYTNYGVAAAAVRLAN
VVLNDAHEVLPVSNYREEYGTYLSYPAVVGRDGILEQLQLELTDEERYKLRRSADFIAEKIKESK

Specific function: Catalyzes the NADP dependent reversible and stereospecific interconversion between 2-ketocarboxylic acids and L-2-hydroxy-carboxylic acids. 2-ketoacids with medium chain length (five to six C-atoms) are the best substrates [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. LDH family [H]

Homologues:

Organism=Homo sapiens, GI47059044, Length=313, Percent_Identity=30.3514376996805, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI221136809, Length=313, Percent_Identity=30.3514376996805, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI15082234, Length=308, Percent_Identity=28.5714285714286, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI5031857, Length=303, Percent_Identity=29.3729372937294, Blast_Score=135, Evalue=5e-32,
Organism=Homo sapiens, GI260099723, Length=303, Percent_Identity=29.3729372937294, Blast_Score=135, Evalue=6e-32,
Organism=Homo sapiens, GI9257228, Length=303, Percent_Identity=28.7128712871287, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI4504973, Length=303, Percent_Identity=28.7128712871287, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI291575128, Length=313, Percent_Identity=28.7539936102236, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI4557032, Length=313, Percent_Identity=28.7539936102236, Blast_Score=131, Evalue=7e-31,
Organism=Homo sapiens, GI207028494, Length=186, Percent_Identity=32.7956989247312, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI260099725, Length=206, Percent_Identity=30.5825242718447, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI260099727, Length=206, Percent_Identity=30.5825242718447, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI103472011, Length=303, Percent_Identity=23.7623762376238, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535107, Length=302, Percent_Identity=26.4900662251656, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550422, Length=307, Percent_Identity=29.9674267100977, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17136226, Length=312, Percent_Identity=26.9230769230769, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: NA

Molecular weight: Translated: 33341; Mature: 33210

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00064 L_LDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKIGIIGMGHVGQAVAQQIITNGYADDLVLIDKNEAKVGADALDFEDAMPNLTSHTNI
CCCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCEE
IVNDYRALKDADVIISALGKIALTDNPDNDRFAELPHNREQVQAVAEAIKASGFNGILIP
EEECHHHHHHHHHHHHHHCCEEECCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
ITNPVDVITALYQEATGLPKAHVIGTGTLLDSARMKRAVGKGLGVDPRSVSGYNLGEHGN
CCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
SQFTAWSTVTVLDQPIKKLAQARNLDLDQINEDARMGGRYVMVGKHYTNYGVAAAAVRLA
CCEEEEEHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHHH
NVVLNDAHEVLPVSNYREEYGTYLSYPAVVGRDGILEQLQLELTDEERYKLRRSADFIAE
HHHHHCCHHHCCCHHHHHHHCCEECCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
KIKESK
HHHCCC
>Mature Secondary Structure 
TRKIGIIGMGHVGQAVAQQIITNGYADDLVLIDKNEAKVGADALDFEDAMPNLTSHTNI
CCEEEEEECCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCHHHHCCCCCCCCEE
IVNDYRALKDADVIISALGKIALTDNPDNDRFAELPHNREQVQAVAEAIKASGFNGILIP
EEECHHHHHHHHHHHHHHCCEEECCCCCCCHHHCCCCCHHHHHHHHHHHHHCCCCEEEEE
ITNPVDVITALYQEATGLPKAHVIGTGTLLDSARMKRAVGKGLGVDPRSVSGYNLGEHGN
CCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
SQFTAWSTVTVLDQPIKKLAQARNLDLDQINEDARMGGRYVMVGKHYTNYGVAAAAVRLA
CCEEEEEHHHHHHHHHHHHHHHHCCCHHHCCHHHHCCCEEEEECCCCCCHHHHHHHHHHH
NVVLNDAHEVLPVSNYREEYGTYLSYPAVVGRDGILEQLQLELTDEERYKLRRSADFIAE
HHHHHCCHHHCCCHHHHHHHCCEECCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHH
KIKESK
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2684788; 7643402 [H]