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The map label for this gene is yflS [H]

Identifier: 116492018

GI number: 116492018

Start: 250738

End: 252156

Strand: Direct

Name: yflS [H]

Synonym: PEPE_0210

Alternate gene names: 116492018

Gene position: 250738-252156 (Clockwise)

Preceding gene: 116492017

Following gene: 116492019

Centisome position: 13.68

GC content: 40.73

Gene sequence:

>1419_bases
ATGACTTTAGAGCCTGTAAAATATAAAAAGTTTATTGCGCCACTAGTTGTAGGTGTGATTCTATGGCTATTATCACCAGT
CAAGCCGGTGGGAGTTTCACTAGCAGCTTGGCATATATTTGCAATTTTTGTTGCTACTATTATTGGCTGTATTACCCAAC
CTTTACCAATCGGTGGGGTAGCGATTGTTGGCTTCAGTATCACCGTTTTAACGGGTGAAGTCCCCATGGATAAAGCTATT
ACCGGTTTTGGTAACGATAGTATTTGGTTAATTGTGATGGCATTTTTTATTTCCCGTGGTTTCATTAAAACTGGTTTGGG
ACGACGAATTGCCTTCCATTTTGTTAGCATGTTTGGTAAGAAAACTTTGGGATTGGCGTATTCTTTGATTGGTGTAGATT
TAGTGTTAGCTCCAGCTACGCCCAGTAACACGGCGCGAGCTGGTGGGATTATGTTCCCAATTATTGAATCGCTAGCTAGT
GCATTCGGATCATCGCCTAAGGATCATACGCAAGGCAAGATTGGTTCGTTCTTGCTGTTCTCAGAATTTCACGGAAACAT
CATTACCGCAGCAATGTTTTTAACTGCAATGGCGGGGAATCCGTTGACTCAATCTTTAGCTAAGCCCCTTGGAATTCATA
TTACATGGATGAATTGGTTCATCGCTGGTGTCGTTCCAGGAATTATTTCTTTAATTGTGGTTCCATTGATTATTTACAAA
ATCTATCCACCAGAAATTAAGGAAACTCCTAACGCTAAAGAATGGTCTAAGAAGCAACTTGATGCGATGGGTAAAGTTAG
TACAGCAGAAAAATTCATGGTTTTAACCTTTATCGTTGCGTTAGTATTATGGATTTTAGGATCATTTTTGGGAATCGATG
CAACTTTGACGGCTTTTATTGCCTTATCTTTATTGCTGGTTACCGGTGTCCTTTCTTGGGGCGATGTTACTAAAGAAACC
GGGGCTTGGAATACGTTAGTTTGGTTTTCGGTACTTGTAATGATGGCTGGGCAATTAAACGAACTCGGATTTATTCCTTG
GCTAAGTAAAACGATTGGTACTTCACTCCATGGAATGAATTGGATGTTAGTATTAGTAATTTTAATTCTGGCATATTTCT
ATAGTCATTATCTTTTCGCTAGTGCTACTGCCCATGTCAGTGCCATGTATGGGGCTTTTCTAGCCGTGGCCGTTTCTTCA
GGAGTACCACCACTACTAGCTGCGTTAATGCTAGGATTTTTTGGTGATTTGATTGGTTCAACCACTCACTATGGAAATGG
ACCTGCTCCCATTCTATTTGGTTCGGGCTTTGTAACGCAAAAAGATTGGTGGCGGATGAATGCCATTCTGGGTGTGGTTT
ACATCGTAATTTGGGTTGGAATTGGCTCACTTTGGATGAAAGTGATTGGTATGTGGTAA

Upstream 100 bases:

>100_bases
AGAAGCTGCCCTAAAATCTGGTAAAGTTACCGCTGAGCAATTTGATCAGTGGGTGGTTCCCATTGAGATGACTAATTATG
ATAAATAGAGGAGTAAAAAG

Downstream 100 bases:

>100_bases
AAAGCCCTTATTATAATCCGTTACTTTAGACGTGTTTTTATATTTCAAATAGATTAGAATGGTTTATGACCCAAAAAAAG
AAGCAGGAGAATTAATAAAT

Product: di- and tricarboxylate transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLEPVKYKKFIAPLVVGVILWLLSPVKPVGVSLAAWHIFAIFVATIIGCITQPLPIGGVAIVGFSITVLTGEVPMDKAI
TGFGNDSIWLIVMAFFISRGFIKTGLGRRIAFHFVSMFGKKTLGLAYSLIGVDLVLAPATPSNTARAGGIMFPIIESLAS
AFGSSPKDHTQGKIGSFLLFSEFHGNIITAAMFLTAMAGNPLTQSLAKPLGIHITWMNWFIAGVVPGIISLIVVPLIIYK
IYPPEIKETPNAKEWSKKQLDAMGKVSTAEKFMVLTFIVALVLWILGSFLGIDATLTAFIALSLLLVTGVLSWGDVTKET
GAWNTLVWFSVLVMMAGQLNELGFIPWLSKTIGTSLHGMNWMLVLVILILAYFYSHYLFASATAHVSAMYGAFLAVAVSS
GVPPLLAALMLGFFGDLIGSTTHYGNGPAPILFGSGFVTQKDWWRMNAILGVVYIVIWVGIGSLWMKVIGMW

Sequences:

>Translated_472_residues
MTLEPVKYKKFIAPLVVGVILWLLSPVKPVGVSLAAWHIFAIFVATIIGCITQPLPIGGVAIVGFSITVLTGEVPMDKAI
TGFGNDSIWLIVMAFFISRGFIKTGLGRRIAFHFVSMFGKKTLGLAYSLIGVDLVLAPATPSNTARAGGIMFPIIESLAS
AFGSSPKDHTQGKIGSFLLFSEFHGNIITAAMFLTAMAGNPLTQSLAKPLGIHITWMNWFIAGVVPGIISLIVVPLIIYK
IYPPEIKETPNAKEWSKKQLDAMGKVSTAEKFMVLTFIVALVLWILGSFLGIDATLTAFIALSLLLVTGVLSWGDVTKET
GAWNTLVWFSVLVMMAGQLNELGFIPWLSKTIGTSLHGMNWMLVLVILILAYFYSHYLFASATAHVSAMYGAFLAVAVSS
GVPPLLAALMLGFFGDLIGSTTHYGNGPAPILFGSGFVTQKDWWRMNAILGVVYIVIWVGIGSLWMKVIGMW
>Mature_471_residues
TLEPVKYKKFIAPLVVGVILWLLSPVKPVGVSLAAWHIFAIFVATIIGCITQPLPIGGVAIVGFSITVLTGEVPMDKAIT
GFGNDSIWLIVMAFFISRGFIKTGLGRRIAFHFVSMFGKKTLGLAYSLIGVDLVLAPATPSNTARAGGIMFPIIESLASA
FGSSPKDHTQGKIGSFLLFSEFHGNIITAAMFLTAMAGNPLTQSLAKPLGIHITWMNWFIAGVVPGIISLIVVPLIIYKI
YPPEIKETPNAKEWSKKQLDAMGKVSTAEKFMVLTFIVALVLWILGSFLGIDATLTAFIALSLLLVTGVLSWGDVTKETG
AWNTLVWFSVLVMMAGQLNELGFIPWLSKTIGTSLHGMNWMLVLVILILAYFYSHYLFASATAHVSAMYGAFLAVAVSSG
VPPLLAALMLGFFGDLIGSTTHYGNGPAPILFGSGFVTQKDWWRMNAILGVVYIVIWVGIGSLWMKVIGMW

Specific function: Might be a malate transporter [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=492, Percent_Identity=36.1788617886179, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1786986, Length=455, Percent_Identity=36.2637362637363, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1789444, Length=483, Percent_Identity=37.0600414078675, Blast_Score=288, Evalue=6e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 50941; Mature: 50809

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEPVKYKKFIAPLVVGVILWLLSPVKPVGVSLAAWHIFAIFVATIIGCITQPLPIGGV
CCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AIVGFSITVLTGEVPMDKAITGFGNDSIWLIVMAFFISRGFIKTGLGRRIAFHFVSMFGK
EEEEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
KTLGLAYSLIGVDLVLAPATPSNTARAGGIMFPIIESLASAFGSSPKDHTQGKIGSFLLF
HHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SEFHGNIITAAMFLTAMAGNPLTQSLAKPLGIHITWMNWFIAGVVPGIISLIVVPLIIYK
HHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
IYPPEIKETPNAKEWSKKQLDAMGKVSTAEKFMVLTFIVALVLWILGSFLGIDATLTAFI
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
ALSLLLVTGVLSWGDVTKETGAWNTLVWFSVLVMMAGQLNELGFIPWLSKTIGTSLHGMN
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHCCHHCCHH
WMLVLVILILAYFYSHYLFASATAHVSAMYGAFLAVAVSSGVPPLLAALMLGFFGDLIGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
TTHYGNGPAPILFGSGFVTQKDWWRMNAILGVVYIVIWVGIGSLWMKVIGMW
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLEPVKYKKFIAPLVVGVILWLLSPVKPVGVSLAAWHIFAIFVATIIGCITQPLPIGGV
CCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCE
AIVGFSITVLTGEVPMDKAITGFGNDSIWLIVMAFFISRGFIKTGLGRRIAFHFVSMFGK
EEEEEEEEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
KTLGLAYSLIGVDLVLAPATPSNTARAGGIMFPIIESLASAFGSSPKDHTQGKIGSFLLF
HHHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
SEFHGNIITAAMFLTAMAGNPLTQSLAKPLGIHITWMNWFIAGVVPGIISLIVVPLIIYK
HHHCCHHHHHHHHHHHHCCCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
IYPPEIKETPNAKEWSKKQLDAMGKVSTAEKFMVLTFIVALVLWILGSFLGIDATLTAFI
CCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
ALSLLLVTGVLSWGDVTKETGAWNTLVWFSVLVMMAGQLNELGFIPWLSKTIGTSLHGMN
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHCCHHCCHH
WMLVLVILILAYFYSHYLFASATAHVSAMYGAFLAVAVSSGVPPLLAALMLGFFGDLIGS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
TTHYGNGPAPILFGSGFVTQKDWWRMNAILGVVYIVIWVGIGSLWMKVIGMW
CCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377; 12949159 [H]