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The map label for this gene is bsdA [H]

Identifier: 116491866

GI number: 116491866

Start: 44872

End: 45768

Strand: Reverse

Name: bsdA [H]

Synonym: PEPE_0048

Alternate gene names: 116491866

Gene position: 45768-44872 (Counterclockwise)

Preceding gene: 116491870

Following gene: 116491865

Centisome position: 2.5

GC content: 41.25

Gene sequence:

>897_bases
ATGGATATTCGTGTCTTACGCTATTTTGTGGTAATTGCTCAGGAACAAAACATTTCACGAGCGGCCACTTTACTTAACGT
GTCCCAACCGGCTTTATCACGCCAAATTGCTGATTTAGAAGATGAATTAGGGACCAAATTATTCACTCGCGCCCATCGCC
ATATTCAACTTACGCAGGAAGGTCATTACCTATTAGAACGGGCGCTACAAATTATTGGTTTAGTTGATAAAACTACTTAT
AACTTACAAAAACAAGATGTAGTTAGTGGGACGCTCGACATTGGAGCAGGAGAAAGTGTGGGGATTATGCCAGTCATGGA
GGCGGCCCAGCAAATCATGCGTCAATACCCCGATGTTCGTGTTAATCTTCGAAGTGCAGATGCGGAACCCATCTTAGCTG
ACCTCGATGCAGGCATTTTAGAATTTGGCATTATTATGGGAAATCGCCCCCTTAATAATTACAACACTTTGGTTTTACCA
GCTAAAAATCATTGGTGCGCCATCATGCGAGCAGATGAACCGCTCGCCCAACAGAAAGAAATCCGACCAATTGATCTAGT
CGGTCGTCCTTTAATTACTTCACGTCAGGCTTACAAACACGGTTTATTCCAAAAATGGAGTAACACCCTTTATGATCAAC
TTAATTTTGTGGGAACTTTCAATTTGGCTTTTAATGGTAGTTTACTAGTCAAAACGGGAGCAGCAATTGCACTTTTATAT
GATCAACTTGTGGACGTTTCACCAACAAGTGGTTTGGTCGCCCGCCCGCTTAATCCTGACATGTCAGAAGCGAACACACT
CATTTGGAGTAAAAATCGAAATTTACCCAATTTAAATCGATTATTTTTAGATACTTTACAAAAAAATTTAGCTCAACAAG
GAGGCTCTGATGCCTAA

Upstream 100 bases:

>100_bases
TTATTGATTGACTCTAGTCTACGGGATCGTTACTATAATGTTAAATACTTATTAAACATCAAAAACAATAACTTTTAGTT
ATGAATTAAAGGAAAATATT

Downstream 100 bases:

>100_bases
ATATACCGTTATTTATAATGAAGATGCTGGAAAAAATGATAATAAATCCATCGCTGAACGCTTTCAGCAAGCGGCTGAAC
GATTGAAGCGATCAACCACC

Product: fhu operon transcription regulator

Products: NA

Alternate protein names: Bsd operon regulatory protein [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MDIRVLRYFVVIAQEQNISRAATLLNVSQPALSRQIADLEDELGTKLFTRAHRHIQLTQEGHYLLERALQIIGLVDKTTY
NLQKQDVVSGTLDIGAGESVGIMPVMEAAQQIMRQYPDVRVNLRSADAEPILADLDAGILEFGIIMGNRPLNNYNTLVLP
AKNHWCAIMRADEPLAQQKEIRPIDLVGRPLITSRQAYKHGLFQKWSNTLYDQLNFVGTFNLAFNGSLLVKTGAAIALLY
DQLVDVSPTSGLVARPLNPDMSEANTLIWSKNRNLPNLNRLFLDTLQKNLAQQGGSDA

Sequences:

>Translated_298_residues
MDIRVLRYFVVIAQEQNISRAATLLNVSQPALSRQIADLEDELGTKLFTRAHRHIQLTQEGHYLLERALQIIGLVDKTTY
NLQKQDVVSGTLDIGAGESVGIMPVMEAAQQIMRQYPDVRVNLRSADAEPILADLDAGILEFGIIMGNRPLNNYNTLVLP
AKNHWCAIMRADEPLAQQKEIRPIDLVGRPLITSRQAYKHGLFQKWSNTLYDQLNFVGTFNLAFNGSLLVKTGAAIALLY
DQLVDVSPTSGLVARPLNPDMSEANTLIWSKNRNLPNLNRLFLDTLQKNLAQQGGSDA
>Mature_298_residues
MDIRVLRYFVVIAQEQNISRAATLLNVSQPALSRQIADLEDELGTKLFTRAHRHIQLTQEGHYLLERALQIIGLVDKTTY
NLQKQDVVSGTLDIGAGESVGIMPVMEAAQQIMRQYPDVRVNLRSADAEPILADLDAGILEFGIIMGNRPLNNYNTLVLP
AKNHWCAIMRADEPLAQQKEIRPIDLVGRPLITSRQAYKHGLFQKWSNTLYDQLNFVGTFNLAFNGSLLVKTGAAIALLY
DQLVDVSPTSGLVARPLNPDMSEANTLIWSKNRNLPNLNRLFLDTLQKNLAQQGGSDA

Specific function: Could be a positive regulator of bsdBCD expression in response to salicylic acid [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=205, Percent_Identity=30.2439024390244, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI145693105, Length=266, Percent_Identity=25.187969924812, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788887, Length=300, Percent_Identity=28.6666666666667, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1790399, Length=194, Percent_Identity=31.9587628865979, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI2367136, Length=176, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787530, Length=198, Percent_Identity=28.2828282828283, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788748, Length=296, Percent_Identity=23.6486486486486, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=272, Percent_Identity=25.3676470588235, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI157672245, Length=185, Percent_Identity=31.3513513513514, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081904, Length=183, Percent_Identity=28.9617486338798, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789204, Length=311, Percent_Identity=25.7234726688103, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1787806, Length=153, Percent_Identity=27.4509803921569, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788706, Length=118, Percent_Identity=29.6610169491525, Blast_Score=62, Evalue=6e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33199; Mature: 33199

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRVLRYFVVIAQEQNISRAATLLNVSQPALSRQIADLEDELGTKLFTRAHRHIQLTQE
CCHHEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
GHYLLERALQIIGLVDKTTYNLQKQDVVSGTLDIGAGESVGIMPVMEAAQQIMRQYPDVR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
VNLRSADAEPILADLDAGILEFGIIMGNRPLNNYNTLVLPAKNHWCAIMRADEPLAQQKE
EEEECCCCCCHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHC
IRPIDLVGRPLITSRQAYKHGLFQKWSNTLYDQLNFVGTFNLAFNGSLLVKTGAAIALLY
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEECCCHHHHHH
DQLVDVSPTSGLVARPLNPDMSEANTLIWSKNRNLPNLNRLFLDTLQKNLAQQGGSDA
HHHHCCCCCCCCEECCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDIRVLRYFVVIAQEQNISRAATLLNVSQPALSRQIADLEDELGTKLFTRAHRHIQLTQE
CCHHEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECC
GHYLLERALQIIGLVDKTTYNLQKQDVVSGTLDIGAGESVGIMPVMEAAQQIMRQYPDVR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHCCEEECCCCCCCCCHHHHHHHHHHHHHCCCEE
VNLRSADAEPILADLDAGILEFGIIMGNRPLNNYNTLVLPAKNHWCAIMRADEPLAQQKE
EEEECCCCCCHHHHHCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEEEEECCCCHHHHHC
IRPIDLVGRPLITSRQAYKHGLFQKWSNTLYDQLNFVGTFNLAFNGSLLVKTGAAIALLY
CCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCEEEEECCCHHHHHH
DQLVDVSPTSGLVARPLNPDMSEANTLIWSKNRNLPNLNRLFLDTLQKNLAQQGGSDA
HHHHCCCCCCCCEECCCCCCHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969502; 9384377 [H]