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The map label for this gene is mtnD

Identifier: 116332639

GI number: 116332639

Start: 251931

End: 252464

Strand: Reverse

Name: mtnD

Synonym: LBJ_4219

Alternate gene names: 116332639

Gene position: 252464-251931 (Counterclockwise)

Preceding gene: 116332640

Following gene: 116332638

Centisome position: 84.22

GC content: 41.95

Gene sequence:

>534_bases
ATGGCGACGATAGTACGACAAAAAGGTTTGGCACCGATCGAGGAAACGAGCGAAGTAAAATCCTTTCTTAAAGAAAGAGG
AATTGATTACGATCATTGGAAGGTGCCGTATAACGCAGCGGATTTAACGGATAAGGAAATACTTGCCGATGTCGAAAAAG
AGGAACTTTTAAAAAAACTGGATGATCGTTTTGAAACTCTCAAAGAAAAAGAAGGTTATCAATCCAGAGATCTGATCGTA
TTACATCCGAACGTTTCGGGATTAAACGACATGCTTGCTAAGTTCGACCGAGTTCACTATCACACCGACGAAGAGGTGAG
ATATATCGTGGACGGTTCCGGCGTTTTCGGTTTTGTATTAAAAGGCGAGAAGTTTCTCGTTCATGTCGTAAAGGACGATT
TTATCTCCGTTCCAAGGAACACGAATCATTGGTTTTACTTGGACGATAAAAAAAGAATCAAAGCGGTTCGTTACTTTCGG
GATATGAGCGGTTGGGTGCCGAACTACGTAGAAGAAACCAATTCCCTGGACTGA

Upstream 100 bases:

>100_bases
TTCCGATATTCGTAAAGCAGGGAGATGTAATTAAAATAGACCTGCGGGATCTCACTTACGTGGAAAGGGTGAGTAGATAA
TACAAGCTGCGGAGATTTTT

Downstream 100 bases:

>100_bases
TATGTCCGTCAAAAAACAACTGGAGAAGCTCTCTATATTGGGGGCTACCTACCATAAAAACGGATGGATGCCGGGAACCG
CCGGTAATCTTTCCGTCCGA

Product: ARD family protein

Products: NA

Alternate protein names: 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase; DHK-MTPene dioxygenase; Acireductone dioxygenase (Fe(2+)-requiring); ARD'; Fe-ARD; Acireductone dioxygenase (Ni(2+)-requiring); ARD; Ni-ARD

Number of amino acids: Translated: 177; Mature: 176

Protein sequence:

>177_residues
MATIVRQKGLAPIEETSEVKSFLKERGIDYDHWKVPYNAADLTDKEILADVEKEELLKKLDDRFETLKEKEGYQSRDLIV
LHPNVSGLNDMLAKFDRVHYHTDEEVRYIVDGSGVFGFVLKGEKFLVHVVKDDFISVPRNTNHWFYLDDKKRIKAVRYFR
DMSGWVPNYVEETNSLD

Sequences:

>Translated_177_residues
MATIVRQKGLAPIEETSEVKSFLKERGIDYDHWKVPYNAADLTDKEILADVEKEELLKKLDDRFETLKEKEGYQSRDLIV
LHPNVSGLNDMLAKFDRVHYHTDEEVRYIVDGSGVFGFVLKGEKFLVHVVKDDFISVPRNTNHWFYLDDKKRIKAVRYFR
DMSGWVPNYVEETNSLD
>Mature_176_residues
ATIVRQKGLAPIEETSEVKSFLKERGIDYDHWKVPYNAADLTDKEILADVEKEELLKKLDDRFETLKEKEGYQSRDLIVL
HPNVSGLNDMLAKFDRVHYHTDEEVRYIVDGSGVFGFVLKGEKFLVHVVKDDFISVPRNTNHWFYLDDKKRIKAVRYFRD
MSGWVPNYVEETNSLD

Specific function: Catalyzes 2 different reactions between oxygene and the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) depending upon the metal bound in the active site. Fe-containing acireductone dioxygenase (Fe-ARD) produces formate and 2-keto-4- me

COG id: COG1791

COG function: function code S; Uncharacterized conserved protein, contains double-stranded beta-helix domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acireductone dioxygenase (ARD) family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MTND_LEPBJ (Q04NC2)

Other databases:

- EMBL:   CP000351
- RefSeq:   YP_802356.1
- ProteinModelPortal:   Q04NC2
- SMR:   Q04NC2
- STRING:   Q04NC2
- GeneID:   4412291
- GenomeReviews:   CP000351_GR
- KEGG:   lbj:LBJ_4219
- eggNOG:   COG1791
- HOGENOM:   HBG660685
- OMA:   CEKNDLI
- PhylomeDB:   Q04NC2
- ProtClustDB:   CLSK575414
- BioCyc:   LBOR355277:LBJ_4219-MONOMER
- HAMAP:   MF_01682
- InterPro:   IPR004313
- InterPro:   IPR011051
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- PANTHER:   PTHR23418

Pfam domain/function: PF03079 ARD; SSF51182 RmlC_like_cupin

EC number: =1.13.11.54; =1.13.11.53

Molecular weight: Translated: 20758; Mature: 20627

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIVRQKGLAPIEETSEVKSFLKERGIDYDHWKVPYNAADLTDKEILADVEKEELLKKL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHCCCHHHHHHHH
DDRFETLKEKEGYQSRDLIVLHPNVSGLNDMLAKFDRVHYHTDEEVRYIVDGSGVFGFVL
HHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEE
KGEKFLVHVVKDDFISVPRNTNHWFYLDDKKRIKAVRYFRDMSGWVPNYVEETNSLD
ECCEEEEEEEHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCC
>Mature Secondary Structure 
ATIVRQKGLAPIEETSEVKSFLKERGIDYDHWKVPYNAADLTDKEILADVEKEELLKKL
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCEECCCCCCCCCHHHHHHCCCHHHHHHHH
DDRFETLKEKEGYQSRDLIVLHPNVSGLNDMLAKFDRVHYHTDEEVRYIVDGSGVFGFVL
HHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEE
KGEKFLVHVVKDDFISVPRNTNHWFYLDDKKRIKAVRYFRDMSGWVPNYVEETNSLD
ECCEEEEEEEHHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA