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The map label for this gene is cobD [H]

Identifier: 116332619

GI number: 116332619

Start: 222332

End: 223300

Strand: Direct

Name: cobD [H]

Synonym: LBJ_4192

Alternate gene names: 116332619

Gene position: 222332-223300 (Clockwise)

Preceding gene: 116332618

Following gene: 116332620

Centisome position: 74.17

GC content: 39.11

Gene sequence:

>969_bases
ATGCCTTGGATCATAACCTTGTCTATTGTCCTCGATTTGATATTCGGAGATCCGAGAAATACACCTCACCCAGTACGAGT
AATCGGAAAATTTGCAAGGATATCTGAAAAATTCTTCAGGACTCACTGTAATTCGGAAAGATTCGCAGGAATTTTGACAT
CTTGTTGTGTGTACACCTTTTCTTTTTCGATACCTTTTTTTTTGATTCAATTTTCGAACAAACTTCACTGGATTTTAGCC
TCATTTTTTTCGATAACGACAATTTACACTACGATTGCAATCCGAGACATGATCGACCATAGTAAAGAAGTTTATGATGC
TTTAGCACAAACCAATCTCTCACTTGCTCGTGTAAAAGTTTCTCGAATCGTCGCAAGAGACACAAAAAACTTAAACCAAC
CAGAAATTATTCGAGCCTGCGTGGAAAGCATCGCTGAAAGTCTTGTAGACGGAATTACAGCCCCTCTTTTTTACGCGGTT
TTCGGAGGCCCTTCTTGGGCGATGCTTTATCGATCAATCAACACATTAGATTCTCTTTTTGGATATAAAAACAATCGATA
TAGAAAATTCGGAAGTTTTCCGGCAAGAATGGATGACTTCGCAAATTATCTACCCGCAAGAATTACATGCTACATACTCG
TTTTTTCATCCTTGGTTCTCGGTTACAACTTTAAGAATTCATTGTATACTCTCCACAGGGACGGAAGAAAACATCCAAGC
CCTAATTCGGGACTCACCGAAGCCGCCGTTGCCGGGGCATTAGAGATACAACTCGGTGGGATCAATTTTTATAACGGAAT
TCAGAACGTTAAACCGAAATTGGGAGATAACAAAAAAGAACTCCGAATAGAACAAATTCTTCAAGCCAACAAGCTGGTTC
TATTATCCTCCATTTTAACCGCAGGATTTTATGTACTTATCTATTCAATCGTTGTTTGGCTTTGGGAAGAATGGAAGCTC
AGAAATTGA

Upstream 100 bases:

>100_bases
TTTTCTTATGATTTGGAAAAATCTTTGAATAGACTTGCCGAACACGTTAGACTCTCTTTAAATATGGATTTAATCTATCA
AACGTTAGGGCTCGGCTAAA

Downstream 100 bases:

>100_bases
AAAACACATTTAAAAAATCAAATATACTTTATGTATTTAGGCACGGAGAAACCGATTGGAACAAAGAAGGTAGACTCCAA
GGACATTTAGAAATTCCCAT

Product: cobalamin biosynthesis protein

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MPWIITLSIVLDLIFGDPRNTPHPVRVIGKFARISEKFFRTHCNSERFAGILTSCCVYTFSFSIPFFLIQFSNKLHWILA
SFFSITTIYTTIAIRDMIDHSKEVYDALAQTNLSLARVKVSRIVARDTKNLNQPEIIRACVESIAESLVDGITAPLFYAV
FGGPSWAMLYRSINTLDSLFGYKNNRYRKFGSFPARMDDFANYLPARITCYILVFSSLVLGYNFKNSLYTLHRDGRKHPS
PNSGLTEAAVAGALEIQLGGINFYNGIQNVKPKLGDNKKELRIEQILQANKLVLLSSILTAGFYVLIYSIVVWLWEEWKL
RN

Sequences:

>Translated_322_residues
MPWIITLSIVLDLIFGDPRNTPHPVRVIGKFARISEKFFRTHCNSERFAGILTSCCVYTFSFSIPFFLIQFSNKLHWILA
SFFSITTIYTTIAIRDMIDHSKEVYDALAQTNLSLARVKVSRIVARDTKNLNQPEIIRACVESIAESLVDGITAPLFYAV
FGGPSWAMLYRSINTLDSLFGYKNNRYRKFGSFPARMDDFANYLPARITCYILVFSSLVLGYNFKNSLYTLHRDGRKHPS
PNSGLTEAAVAGALEIQLGGINFYNGIQNVKPKLGDNKKELRIEQILQANKLVLLSSILTAGFYVLIYSIVVWLWEEWKL
RN
>Mature_321_residues
PWIITLSIVLDLIFGDPRNTPHPVRVIGKFARISEKFFRTHCNSERFAGILTSCCVYTFSFSIPFFLIQFSNKLHWILAS
FFSITTIYTTIAIRDMIDHSKEVYDALAQTNLSLARVKVSRIVARDTKNLNQPEIIRACVESIAESLVDGITAPLFYAVF
GGPSWAMLYRSINTLDSLFGYKNNRYRKFGSFPARMDDFANYLPARITCYILVFSSLVLGYNFKNSLYTLHRDGRKHPSP
NSGLTEAAVAGALEIQLGGINFYNGIQNVKPKLGDNKKELRIEQILQANKLVLLSSILTAGFYVLIYSIVVWLWEEWKLR
N

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 36666; Mature: 36535

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWIITLSIVLDLIFGDPRNTPHPVRVIGKFARISEKFFRTHCNSERFAGILTSCCVYTF
CCEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SFSIPFFLIQFSNKLHWILASFFSITTIYTTIAIRDMIDHSKEVYDALAQTNLSLARVKV
HHCCHHHEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SRIVARDTKNLNQPEIIRACVESIAESLVDGITAPLFYAVFGGPSWAMLYRSINTLDSLF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GYKNNRYRKFGSFPARMDDFANYLPARITCYILVFSSLVLGYNFKNSLYTLHRDGRKHPS
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCC
PNSGLTEAAVAGALEIQLGGINFYNGIQNVKPKLGDNKKELRIEQILQANKLVLLSSILT
CCCCCHHHHHHCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGFYVLIYSIVVWLWEEWKLRN
HHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PWIITLSIVLDLIFGDPRNTPHPVRVIGKFARISEKFFRTHCNSERFAGILTSCCVYTF
CEEEEHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
SFSIPFFLIQFSNKLHWILASFFSITTIYTTIAIRDMIDHSKEVYDALAQTNLSLARVKV
HHCCHHHEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
SRIVARDTKNLNQPEIIRACVESIAESLVDGITAPLFYAVFGGPSWAMLYRSINTLDSLF
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GYKNNRYRKFGSFPARMDDFANYLPARITCYILVFSSLVLGYNFKNSLYTLHRDGRKHPS
CCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHEEEECCCCCCCC
PNSGLTEAAVAGALEIQLGGINFYNGIQNVKPKLGDNKKELRIEQILQANKLVLLSSILT
CCCCCHHHHHHCEEEEEECCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AGFYVLIYSIVVWLWEEWKLRN
HHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA