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The map label for this gene is cobB [H]

Identifier: 116332615

GI number: 116332615

Start: 216954

End: 218333

Strand: Direct

Name: cobB [H]

Synonym: LBJ_4188

Alternate gene names: 116332615

Gene position: 216954-218333 (Clockwise)

Preceding gene: 116332614

Following gene: 116332616

Centisome position: 72.38

GC content: 43.84

Gene sequence:

>1380_bases
GTGAACGTTTTATATACAGAAATCAAAATTCCAAGAATCGTAATCGCAGGAACAGGAAGCGGCGTCGGAAAAACCACGGT
AGTTCTTGCACTCACGCAAGCGTTTCAAAAAAGGGGATTGAAGGTTGCAACTTTCAAATGCGGCCCCGACTATTTGGATC
CGACGTATCATTCTCACATAACGAGAAAAACATGCCATAACCTAGACGGATGGTTGATGGGGAAACAATCCGTCTTGAAC
GTCCTTCATAAAGCGTGTCATAACGCAGATATTGCGATCATCGAGGGAGTAATGGGATTGTTCGACGGACACTCTCCGAA
TTCGGAAGCCGGTTCCACTGCCGAGATAGCCAAATGGACCTCCTCACCCGTATTAGTAGTCATAGACGCAAGCGGAATGG
CAAGAACCATCGCCGCAATTCTAAAAGGATTGAAAACCTTCGACCCAGATCTCAACCTCGCAGGAGCTTTTACCAATTTT
ATCGGAAGTAAATCACATACCCAACTTCTACGGAAAGCATCCTCGGAAATTCCAATCCTTGGTGGTTTTTCAAAACACCC
ACAACAGGCATTTCCGGAAAGACATCTTGGTTTATACTCCGTCTCAAAGGAAAATTTATCCGAAGAAAAATTTGTATTTT
GGGGAGAAGAAGGGGAAAAACAGCTAGAAATCGATTCCGTTCTAAAAATTGCAGGTTCAGCCCCGAAGATCTCAATCCCG
GCTTCAAAAATAAAAACAATTTCAGGCCGTTGCAAAATCGGAATCAGTATGGATTCCGCATTCCATTTTTATTACGAAGA
GAATTTAATTCTACTACAAGAAGCCGGAGCCGAACTTGTATTTTTTTCCCCCATCTCCGACTCCAGACTTCCTCAAGTGG
ACGGACTTTACATCGGAGGAGGGTATCCGGAACTTTTTGCCTCCGCCTTATCGAAAAACAAATCCTTATTAAACGACATT
CGAGAATTCTCATATAAAAAACGACCGATTTACGCCGAGTGCGGCGGACTCATGTATCTTTCAAAAAAAATCCGACTCGT
TTCGGGAGAATCCTATCCGATGGCGGGTTTGATTCCGGGAACCGCCATCATGAACGAAGAATTAAAAGCCCTCGGCTACG
TGGAAGTCGTCACAAAGCAAAAAACGTTTTTTGGAGAAGCAGGGCTTAGGTTCAGAGGTCACCAGTTTCGTTATTCAAAT
TTCGAACTCGACGAACCGCACTCTATAGAACACGTCTACGGACTAAGAAAAAGAAACGAAGTAAGTGAAGAGGGATATTT
TCACAATTGTGTTCTTGCCTCCTATATACACGCTCACTGGGCGTCCAATCCTATTTTACCGGAAGGATTTATCCGATCCT
GTCTACAAGGGCTAAAATGA

Upstream 100 bases:

>100_bases
CGGAAACTCTGATGAACTACGCCGATTTAGTCAGCGTGACCGAAGCCAAAAAACATCCGTATCGAGACGGAATTCCCGCA
CAACAAGGAATCGATTTTTA

Downstream 100 bases:

>100_bases
TCCATACCCCTCTTATTACGAACGATTCCACATCCTGGCTTATTGCAAACTTCGAAGAGCCGCATAACGTTCTAAGTTGG
GCAGTAATTGGCGGAGGTTG

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MNVLYTEIKIPRIVIAGTGSGVGKTTVVLALTQAFQKRGLKVATFKCGPDYLDPTYHSHITRKTCHNLDGWLMGKQSVLN
VLHKACHNADIAIIEGVMGLFDGHSPNSEAGSTAEIAKWTSSPVLVVIDASGMARTIAAILKGLKTFDPDLNLAGAFTNF
IGSKSHTQLLRKASSEIPILGGFSKHPQQAFPERHLGLYSVSKENLSEEKFVFWGEEGEKQLEIDSVLKIAGSAPKISIP
ASKIKTISGRCKIGISMDSAFHFYYEENLILLQEAGAELVFFSPISDSRLPQVDGLYIGGGYPELFASALSKNKSLLNDI
REFSYKKRPIYAECGGLMYLSKKIRLVSGESYPMAGLIPGTAIMNEELKALGYVEVVTKQKTFFGEAGLRFRGHQFRYSN
FELDEPHSIEHVYGLRKRNEVSEEGYFHNCVLASYIHAHWASNPILPEGFIRSCLQGLK

Sequences:

>Translated_459_residues
MNVLYTEIKIPRIVIAGTGSGVGKTTVVLALTQAFQKRGLKVATFKCGPDYLDPTYHSHITRKTCHNLDGWLMGKQSVLN
VLHKACHNADIAIIEGVMGLFDGHSPNSEAGSTAEIAKWTSSPVLVVIDASGMARTIAAILKGLKTFDPDLNLAGAFTNF
IGSKSHTQLLRKASSEIPILGGFSKHPQQAFPERHLGLYSVSKENLSEEKFVFWGEEGEKQLEIDSVLKIAGSAPKISIP
ASKIKTISGRCKIGISMDSAFHFYYEENLILLQEAGAELVFFSPISDSRLPQVDGLYIGGGYPELFASALSKNKSLLNDI
REFSYKKRPIYAECGGLMYLSKKIRLVSGESYPMAGLIPGTAIMNEELKALGYVEVVTKQKTFFGEAGLRFRGHQFRYSN
FELDEPHSIEHVYGLRKRNEVSEEGYFHNCVLASYIHAHWASNPILPEGFIRSCLQGLK
>Mature_459_residues
MNVLYTEIKIPRIVIAGTGSGVGKTTVVLALTQAFQKRGLKVATFKCGPDYLDPTYHSHITRKTCHNLDGWLMGKQSVLN
VLHKACHNADIAIIEGVMGLFDGHSPNSEAGSTAEIAKWTSSPVLVVIDASGMARTIAAILKGLKTFDPDLNLAGAFTNF
IGSKSHTQLLRKASSEIPILGGFSKHPQQAFPERHLGLYSVSKENLSEEKFVFWGEEGEKQLEIDSVLKIAGSAPKISIP
ASKIKTISGRCKIGISMDSAFHFYYEENLILLQEAGAELVFFSPISDSRLPQVDGLYIGGGYPELFASALSKNKSLLNDI
REFSYKKRPIYAECGGLMYLSKKIRLVSGESYPMAGLIPGTAIMNEELKALGYVEVVTKQKTFFGEAGLRFRGHQFRYSN
FELDEPHSIEHVYGLRKRNEVSEEGYFHNCVLASYIHAHWASNPILPEGFIRSCLQGLK

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 50584; Mature: 50584

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00501 SPASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLYTEIKIPRIVIAGTGSGVGKTTVVLALTQAFQKRGLKVATFKCGPDYLDPTYHSHI
CCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
TRKTCHNLDGWLMGKQSVLNVLHKACHNADIAIIEGVMGLFDGHSPNSEAGSTAEIAKWT
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
SSPVLVVIDASGMARTIAAILKGLKTFDPDLNLAGAFTNFIGSKSHTQLLRKASSEIPIL
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCEE
GGFSKHPQQAFPERHLGLYSVSKENLSEEKFVFWGEEGEKQLEIDSVLKIAGSAPKISIP
CCCCCCCHHHCCHHHCCEEEECHHCCCCCEEEEECCCCCCEECHHHHHHHCCCCCEEECC
ASKIKTISGRCKIGISMDSAFHFYYEENLILLQEAGAELVFFSPISDSRLPQVDGLYIGG
HHHHHCCCCCEEEEEEECCEEEEEEECCEEEEEECCCCEEEECCCCCCCCCCCCEEEECC
GYPELFASALSKNKSLLNDIREFSYKKRPIYAECGGLMYLSKKIRLVSGESYPMAGLIPG
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHCCCEEEEECEEEEEECCCCCCCCCCCC
TAIMNEELKALGYVEVVTKQKTFFGEAGLRFRGHQFRYSNFELDEPHSIEHVYGLRKRNE
HHHHHHHHHHCCHHHEEEHHHHHHCCCCCEECCCEEEECCCCCCCCCCHHHHHHHHHHCC
VSEEGYFHNCVLASYIHAHWASNPILPEGFIRSCLQGLK
CCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MNVLYTEIKIPRIVIAGTGSGVGKTTVVLALTQAFQKRGLKVATFKCGPDYLDPTYHSHI
CCEEEEEEECCEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHH
TRKTCHNLDGWLMGKQSVLNVLHKACHNADIAIIEGVMGLFDGHSPNSEAGSTAEIAKWT
HHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCC
SSPVLVVIDASGMARTIAAILKGLKTFDPDLNLAGAFTNFIGSKSHTQLLRKASSEIPIL
CCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCEE
GGFSKHPQQAFPERHLGLYSVSKENLSEEKFVFWGEEGEKQLEIDSVLKIAGSAPKISIP
CCCCCCCHHHCCHHHCCEEEECHHCCCCCEEEEECCCCCCEECHHHHHHHCCCCCEEECC
ASKIKTISGRCKIGISMDSAFHFYYEENLILLQEAGAELVFFSPISDSRLPQVDGLYIGG
HHHHHCCCCCEEEEEEECCEEEEEEECCEEEEEECCCCEEEECCCCCCCCCCCCEEEECC
GYPELFASALSKNKSLLNDIREFSYKKRPIYAECGGLMYLSKKIRLVSGESYPMAGLIPG
CCHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHCCCEEEEECEEEEEECCCCCCCCCCCC
TAIMNEELKALGYVEVVTKQKTFFGEAGLRFRGHQFRYSNFELDEPHSIEHVYGLRKRNE
HHHHHHHHHHCCHHHEEEHHHHHHCCCCCEECCCEEEECCCCCCCCCCHHHHHHHHHHCC
VSEEGYFHNCVLASYIHAHWASNPILPEGFIRSCLQGLK
CCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA