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The map label for this gene is cobF [H]

Identifier: 116332610

GI number: 116332610

Start: 212287

End: 213051

Strand: Direct

Name: cobF [H]

Synonym: LBJ_4183

Alternate gene names: 116332610

Gene position: 212287-213051 (Clockwise)

Preceding gene: 116332609

Following gene: 116332611

Centisome position: 70.82

GC content: 41.96

Gene sequence:

>765_bases
ATGAGTTCCGGTAAATATGGAAAACTTTACGGAGTTGGAGTCGGTCCAGGGGCCACTGACTTGATCACTCTCAGAGCAGT
GCATATTTTAAATTCGGTCTCAGTCCTAGCCATTCCCAAAAGTAGCGAACATCTGGAACCTTTCGCCTGGAGAGTTTGTT
CTCCGATTGTGAGAGAAAATTCTTCCCAAGAAAAATTGTTTCTTCATTTTCCTATGACGAAAAATCCGAAGATCCTCATC
CCTGCTTGGGATAAGGCATTTTCAGAAATCGGGAAACGTTTGGAAAAGAAGCGTAACGTGGCTTTCATCACCCAGGGCGA
TCCTTCCGTCTATAGTTCCTGGAGTTATCTTTTAGAAGAAGCAAACAATCGTTGGCCTGGGATCGAAGTGGAAGTCGTTC
CGGCAGTTTCGTCCATCACCGCAATTCCCGCGATTCTTCAAACCCCTCTTGCTGATGGAAGAGAACGTTTCTGCGTGGTT
CCAGGAACTTACGGTTTGAAGGACCTTCCCGAACTTATACGACATTTTGATACGATCGTTCTTATAAAAGTAGGACGAGT
CATTCCAAAACTTGTTTCTATATTAAAGGAATTGAATCTCCTACAGAACGCAAATTACGTTTCTTACGGTACGACAGATC
GTCAAAAAATCGTGAAAAATATAGAAACAATTCAAAATGAAAACTGCGATTATTTTTCGATGGTGATTATTTCCATTCGA
AAGCGCAAAGGCGCATTAAAAGGGCAAAATATTGAAACGGAATAG

Upstream 100 bases:

>100_bases
TAAACATTTCCAGAGGACAACCTCTTGCGAATTATCTTAAATACGAAGCTCTAAACCCAATTCATATTTTTAAAATCACA
AAACCGGACGGATTTTCCGC

Downstream 100 bases:

>100_bases
GAAACCATATTCCGTTTTTGCAATAACCAAACACGGACTTGAAATAGCCAATAGAATTCAATCCGCTTGGAAAGAAGTAG
ATCTTTTCGTTTCTTCCAAG

Product: precorrin-6A synthase (deacetylating)

Products: NA

Alternate protein names: S-adenosyl-L-methionine--cobalt-precorrin-2 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MSSGKYGKLYGVGVGPGATDLITLRAVHILNSVSVLAIPKSSEHLEPFAWRVCSPIVRENSSQEKLFLHFPMTKNPKILI
PAWDKAFSEIGKRLEKKRNVAFITQGDPSVYSSWSYLLEEANNRWPGIEVEVVPAVSSITAIPAILQTPLADGRERFCVV
PGTYGLKDLPELIRHFDTIVLIKVGRVIPKLVSILKELNLLQNANYVSYGTTDRQKIVKNIETIQNENCDYFSMVIISIR
KRKGALKGQNIETE

Sequences:

>Translated_254_residues
MSSGKYGKLYGVGVGPGATDLITLRAVHILNSVSVLAIPKSSEHLEPFAWRVCSPIVRENSSQEKLFLHFPMTKNPKILI
PAWDKAFSEIGKRLEKKRNVAFITQGDPSVYSSWSYLLEEANNRWPGIEVEVVPAVSSITAIPAILQTPLADGRERFCVV
PGTYGLKDLPELIRHFDTIVLIKVGRVIPKLVSILKELNLLQNANYVSYGTTDRQKIVKNIETIQNENCDYFSMVIISIR
KRKGALKGQNIETE
>Mature_253_residues
SSGKYGKLYGVGVGPGATDLITLRAVHILNSVSVLAIPKSSEHLEPFAWRVCSPIVRENSSQEKLFLHFPMTKNPKILIP
AWDKAFSEIGKRLEKKRNVAFITQGDPSVYSSWSYLLEEANNRWPGIEVEVVPAVSSITAIPAILQTPLADGRERFCVVP
GTYGLKDLPELIRHFDTIVLIKVGRVIPKLVSILKELNLLQNANYVSYGTTDRQKIVKNIETIQNENCDYFSMVIISIRK
RKGALKGQNIETE

Specific function: Methylates cobalt-precorrin-2 at the C-20 position to produce cobalt-precorrin-3A in the anaerobic cobalamin biosynthesis pathway [H]

COG id: COG2243

COG function: function code H; Precorrin-2 methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR012382
- InterPro:   IPR006364
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: =2.1.1.151 [H]

Molecular weight: Translated: 28391; Mature: 28260

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGKYGKLYGVGVGPGATDLITLRAVHILNSVSVLAIPKSSEHLEPFAWRVCSPIVREN
CCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
SSQEKLFLHFPMTKNPKILIPAWDKAFSEIGKRLEKKRNVAFITQGDPSVYSSWSYLLEE
CCCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
ANNRWPGIEVEVVPAVSSITAIPAILQTPLADGRERFCVVPGTYGLKDLPELIRHFDTIV
HCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIKVGRVIPKLVSILKELNLLQNANYVSYGTTDRQKIVKNIETIQNENCDYFSMVIISIR
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KRKGALKGQNIETE
HHCCCCCCCCCCCC
>Mature Secondary Structure 
SSGKYGKLYGVGVGPGATDLITLRAVHILNSVSVLAIPKSSEHLEPFAWRVCSPIVREN
CCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHCC
SSQEKLFLHFPMTKNPKILIPAWDKAFSEIGKRLEKKRNVAFITQGDPSVYSSWSYLLEE
CCCCEEEEECCCCCCCEEEEECHHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHH
ANNRWPGIEVEVVPAVSSITAIPAILQTPLADGRERFCVVPGTYGLKDLPELIRHFDTIV
HCCCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHH
LIKVGRVIPKLVSILKELNLLQNANYVSYGTTDRQKIVKNIETIQNENCDYFSMVIISIR
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
KRKGALKGQNIETE
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501034; 11677609 [H]