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The map label for this gene is algI [H]

Identifier: 116332578

GI number: 116332578

Start: 170867

End: 172282

Strand: Reverse

Name: algI [H]

Synonym: LBJ_4150

Alternate gene names: 116332578

Gene position: 172282-170867 (Counterclockwise)

Preceding gene: 116332579

Following gene: 116332577

Centisome position: 57.47

GC content: 40.25

Gene sequence:

>1416_bases
ATGCTTTTTAACTCGCTTCACTTTTTGTTTTTTTTTCCCGTTGTACTCGTCATCAATCATTTGCTCAAGGGGAAGATCCA
AAGGATTTTTCTTCTCGGAACTAGTTTTTATTTTTATATGTCCTGGAGAAAAGAATTCATCATTCTTTTGCTCTATTCGA
TCGTTATCGATTATTTTGCTTCTCTCAAAATCCAAGCGGCGGCTCCTGGTTCAAAAAAAAGAAAAATTTGGCTTTTGCTG
TCCCTAGTCACGAATCTGGGGTTACTCGCCTACTTTAAGTATACGAACTTTCTTTTAGGAGTTGTGAACGATCTTACTCC
CGCCGCCGGATTTAAGTTTGCGTATTATGATATCGTTCTTCCGGTCGGGATTTCTTTTTATACGTTTCAATCTTTGAGTT
ATACGATCGACGTATATCGGGGACAAATAGAAGCGAAAAAGTCCTTTTTGGATTTTGCTCTTTATGTTTCCTTTTTTCCT
CAATTGGTCGCGGGGCCGATTGTTCGTGCGCAGACATTCTTTCGGGATTTGGAATTTCCTCTTCCGGTTCGAAAGGAAGA
CATACAAATTGCGTTTTGTCAGATTCTAATCGGATTTACGCGTAAGATCGTTTTTGCGGATAATCTCGCAAAGGTCGTGG
ACTCCACGTTTGCAAATTATGCGACTCTCAATCCGATTGAAATTTGGACCGGAGCACTCGCATTCGGTTGGCAGATTTAC
TTCGACTTTGCGGGTTATACGGACATTGCAATCGGTGTGGCTCGTCTTTTCGGATATAAGTTCGATCCGAATTTCAATTT
CCCCATGGTCGCGAGAAACATCACGGATCACTGGTCTCGTTGGCATATTTCATTTTCCACTTGGATTCGGGATTATATCT
TCATTCCACTCGGAGGATCGAGAGGAACGGCGCTTCTGACTTATAGAAACATTTTCATCACTTGGTTTTTTGCCGGAGCC
TGGCACGGGGCGGCCTACCATTTTATTAGTTGGGGATTTTGGCAAGGGGTTATGATTTTTACGCATAGGGAATATGCCAA
AACAAAAATTGCCGCCTTACTGAACGAAAAGGGAGGAATCGTCTACGATATCTGCGCGAGAATTTTTACTATGTTTTGCT
TAACGTTTGGATTTGTTATGTTTCGTGCTGAAACAATGGAAAAGGCCGTTTTGATGATGAAATCTCTTCTCTTTTTGAGT
TCGAACGGATTTGTGGGGGTTAAAGAATATTCGAATTATACATACGGAATTCTTTTGTTCGTTTGTTTTGCGGCTTCTTA
TATATTCGAAAAAAATAATATAGAAAAAATCGTTCGAAGTCAATGGAAATTCATTCTATTCTTCCTTGCAAACGTCTTTA
TGCTTTTGGTTTTCGGTATTACGGAAAGCCAGAGTTTCCTCTACTTTGCGTTTTAG

Upstream 100 bases:

>100_bases
TATTTTTACCGAGGATTTCAAATCCGTAAATTGAAGTTGTAAGGATATTCCGTCTTGAAAAACTGCTCTTCAGATCTTTA
AGATCCGATTTAGACGAAAC

Downstream 100 bases:

>100_bases
GAAAACATCCGTTATGAAAGAATACCTTGCATTTTTCAAAGACCCAAAATTCTGGATTCCGGTTTTAGTTTTGATTTTTT
TAGAGACCGGAATGCAATTT

Product: DltB-related membrane protein

Products: NA

Alternate protein names: Alginate biosynthesis protein AlgI [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MLFNSLHFLFFFPVVLVINHLLKGKIQRIFLLGTSFYFYMSWRKEFIILLLYSIVIDYFASLKIQAAAPGSKKRKIWLLL
SLVTNLGLLAYFKYTNFLLGVVNDLTPAAGFKFAYYDIVLPVGISFYTFQSLSYTIDVYRGQIEAKKSFLDFALYVSFFP
QLVAGPIVRAQTFFRDLEFPLPVRKEDIQIAFCQILIGFTRKIVFADNLAKVVDSTFANYATLNPIEIWTGALAFGWQIY
FDFAGYTDIAIGVARLFGYKFDPNFNFPMVARNITDHWSRWHISFSTWIRDYIFIPLGGSRGTALLTYRNIFITWFFAGA
WHGAAYHFISWGFWQGVMIFTHREYAKTKIAALLNEKGGIVYDICARIFTMFCLTFGFVMFRAETMEKAVLMMKSLLFLS
SNGFVGVKEYSNYTYGILLFVCFAASYIFEKNNIEKIVRSQWKFILFFLANVFMLLVFGITESQSFLYFAF

Sequences:

>Translated_471_residues
MLFNSLHFLFFFPVVLVINHLLKGKIQRIFLLGTSFYFYMSWRKEFIILLLYSIVIDYFASLKIQAAAPGSKKRKIWLLL
SLVTNLGLLAYFKYTNFLLGVVNDLTPAAGFKFAYYDIVLPVGISFYTFQSLSYTIDVYRGQIEAKKSFLDFALYVSFFP
QLVAGPIVRAQTFFRDLEFPLPVRKEDIQIAFCQILIGFTRKIVFADNLAKVVDSTFANYATLNPIEIWTGALAFGWQIY
FDFAGYTDIAIGVARLFGYKFDPNFNFPMVARNITDHWSRWHISFSTWIRDYIFIPLGGSRGTALLTYRNIFITWFFAGA
WHGAAYHFISWGFWQGVMIFTHREYAKTKIAALLNEKGGIVYDICARIFTMFCLTFGFVMFRAETMEKAVLMMKSLLFLS
SNGFVGVKEYSNYTYGILLFVCFAASYIFEKNNIEKIVRSQWKFILFFLANVFMLLVFGITESQSFLYFAF
>Mature_471_residues
MLFNSLHFLFFFPVVLVINHLLKGKIQRIFLLGTSFYFYMSWRKEFIILLLYSIVIDYFASLKIQAAAPGSKKRKIWLLL
SLVTNLGLLAYFKYTNFLLGVVNDLTPAAGFKFAYYDIVLPVGISFYTFQSLSYTIDVYRGQIEAKKSFLDFALYVSFFP
QLVAGPIVRAQTFFRDLEFPLPVRKEDIQIAFCQILIGFTRKIVFADNLAKVVDSTFANYATLNPIEIWTGALAFGWQIY
FDFAGYTDIAIGVARLFGYKFDPNFNFPMVARNITDHWSRWHISFSTWIRDYIFIPLGGSRGTALLTYRNIFITWFFAGA
WHGAAYHFISWGFWQGVMIFTHREYAKTKIAALLNEKGGIVYDICARIFTMFCLTFGFVMFRAETMEKAVLMMKSLLFLS
SNGFVGVKEYSNYTYGILLFVCFAASYIFEKNNIEKIVRSQWKFILFFLANVFMLLVFGITESQSFLYFAF

Specific function: Together with AlgJ and AlgF, forms an inner membrane complex which probably interacts with the alginate polymerization- transport complex and adds acetyl groups at the O-2 and O-3 positions of polymannuronic acid. Acetylation of alginate is important for

COG id: COG1696

COG function: function code M; Predicted membrane protein involved in D-alanine export

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane-bound acyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004299 [H]

Pfam domain/function: PF03062 MBOAT [H]

EC number: NA

Molecular weight: Translated: 54618; Mature: 54618

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFNSLHFLFFFPVVLVINHLLKGKIQRIFLLGTSFYFYMSWRKEFIILLLYSIVIDYFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHEEHHHHHHHHHHHHHHHHHHHHHH
SLKIQAAAPGSKKRKIWLLLSLVTNLGLLAYFKYTNFLLGVVNDLTPAAGFKFAYYDIVL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHEEEEH
PVGISFYTFQSLSYTIDVYRGQIEAKKSFLDFALYVSFFPQLVAGPIVRAQTFFRDLEFP
HCCCHHEEHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCC
LPVRKEDIQIAFCQILIGFTRKIVFADNLAKVVDSTFANYATLNPIEIWTGALAFGWQIY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEE
FDFAGYTDIAIGVARLFGYKFDPNFNFPMVARNITDHWSRWHISFSTWIRDYIFIPLGGS
EEECCCHHHHHHHHHHHCEEECCCCCCCHHCCCHHHCCCEEEEEHHHHHHEEEEEEECCC
RGTALLTYRNIFITWFFAGAWHGAAYHFISWGFWQGVMIFTHREYAKTKIAALLNEKGGI
CCCEEEEEHHEEEEEEEHHCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCC
VYDICARIFTMFCLTFGFVMFRAETMEKAVLMMKSLLFLSSNGFVGVKEYSNYTYGILLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHCCCCHHHHHHH
VCFAASYIFEKNNIEKIVRSQWKFILFFLANVFMLLVFGITESQSFLYFAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC
>Mature Secondary Structure
MLFNSLHFLFFFPVVLVINHLLKGKIQRIFLLGTSFYFYMSWRKEFIILLLYSIVIDYFA
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECHHEEHHHHHHHHHHHHHHHHHHHHHH
SLKIQAAAPGSKKRKIWLLLSLVTNLGLLAYFKYTNFLLGVVNDLTPAAGFKFAYYDIVL
CCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHEEEEH
PVGISFYTFQSLSYTIDVYRGQIEAKKSFLDFALYVSFFPQLVAGPIVRAQTFFRDLEFP
HCCCHHEEHHHHEEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCC
LPVRKEDIQIAFCQILIGFTRKIVFADNLAKVVDSTFANYATLNPIEIWTGALAFGWQIY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEE
FDFAGYTDIAIGVARLFGYKFDPNFNFPMVARNITDHWSRWHISFSTWIRDYIFIPLGGS
EEECCCHHHHHHHHHHHCEEECCCCCCCHHCCCHHHCCCEEEEEHHHHHHEEEEEEECCC
RGTALLTYRNIFITWFFAGAWHGAAYHFISWGFWQGVMIFTHREYAKTKIAALLNEKGGI
CCCEEEEEHHEEEEEEEHHCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHCCCCCC
VYDICARIFTMFCLTFGFVMFRAETMEKAVLMMKSLLFLSSNGFVGVKEYSNYTYGILLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHCCCCHHHHHHH
VCFAASYIFEKNNIEKIVRSQWKFILFFLANVFMLLVFGITESQSFLYFAF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8636017; 10984043; 12003941; 11208804; 11179370 [H]