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The map label for this gene is rsbU [H]

Identifier: 116332531

GI number: 116332531

Start: 109141

End: 110889

Strand: Reverse

Name: rsbU [H]

Synonym: LBJ_4095

Alternate gene names: 116332531

Gene position: 110889-109141 (Counterclockwise)

Preceding gene: 116332532

Following gene: 116332529

Centisome position: 36.99

GC content: 40.88

Gene sequence:

>1749_bases
ATGAGTGAAAGGCAGTTATCCTTATCTTTAATTTCCAACATTACGGCGAAAATTAACTCGACCGACAACCTGGAAGAACT
TCTTAACATCATCATAGAAACCACAAAAGACGTCCTCAACACAGAAGGTTGTTCCCTCCTTTTGTATGATCGGAACGAAG
ATTGTCTTGTTTTCAATATTGCTAAAGGCACAAAAGAGAAATCCCTTACGGAACTGAAAGTCCCAAGAGGGAAAGGGATT
GCGGGAATGGTTCTAGAGACATTGGAACCCGTAATCGTGAACGACGCAAAAAACGATCCTCGAATTTATAGAAACATCGA
CGAGACCGTGGGATTCGTTACCTATAATCTTATCTGTGTTCCGATGAGCACTCAAGGGGAAATTCAGGGTGTATTGGAAG
CGGTCAATTCTCTAGAAAGAAAAGAATTTACGAACAAAGACATTAAGATTCTTCAATATCTTTCGGATCTTGCCGCGATC
GCAATTCGAAATCGAAGACTGATTCGAGATTTGAAAAGCCGCGCAAACGAACTTGATTGTTTGTTTCAACTCAGTCAGGC
GATCTCGAATATCTCGGAGATGGATCAATTTTTCAATCTCACCGTAAATTCCGTTTCGGAAATTATGGGAGCAGAAAGAG
TTTCTTTAATCTTTTTCAACCCAAAGAACGGAAAATACGAACTCAAAAAAAGTATCGGCTTTAGTCTTAAAGAAGAAGAA
CATTTCATCAACGAAAAAGAAGGCGTAATCCGAAAGGTTATCGAAACCGGTAAACCGATTCTAGTCCAAAACACCTCTTC
TATCATGGAAGACTGTGTTCGTCCCGAAAGGTACAAAACCAAATCTTTTATTTCCGTCCCGATCCGCCAGGACGGAAAGA
TCATCGGAATCCTAAATTCTGCGGATAAAAAATCCGGAAACAATTTCGACAATGCGGATCAAAACGTTTTGAGTACGATT
TCCAACCAGATAGCGGAAGCTTACAATTCCCTTCTTTCCAAGGAACAAAAAGAAAAACTCAATTCGATCCACAGAGATAT
GCAGATCGCTTCCCAGATCCAAGTAAATTCCTTACCAAATATTCCTAAGAAGATCCAAGGTTTGGAATTGGAAACGAGTT
ACATCGCTTCGAAAGAAATCGGAGGCGATTTTTACGATCTAATCTATCATAATCCGGACGAAGTCAGTGTTCTCATCGCA
GACGTTTCTGGAAAAGGAATCGCCGCCGCGCTCTTTATGGAATTTTCCAAAACGATCATCGCAGGAGAAGTTTCCAGAAA
TTCCTCGACTAGTATCAGTTTGATGAGTGCAAACCGAATCATCCAGGAAAAATCCGGATATTTTATGTTCGTTACAGTAA
TGCTCGCAAGAATCAATATGGCGAAACGAAAGATTTGTTATTCTAGTGCAGGCCATAACGAACAACTCCTTTATAAAGCC
AAAGAAAAAAATGTCGTCAGCCTTTCCGGCAAAGGAATGCCTCTCGGAATTAAAGAATCAGAGATTGACGAACACGAGCT
CGATTATTATCCCGGTGATCTTTTAATTCTCTATACGGACGGGGTCAGCGAGGCTATGAACGAATCGAACGAGATGTACG
GTCTTGAAAATCTCACTAAGCTCATCGAACGAAATGGAGATATGCCTCTTGGAGCTTTAAAGGAACTCATCATCGATACG
ACTGATGCGTTTCGAGGAGATGCAGATCCACACGACGATTATACATTGTTTATGATTCGTCTTCCGTAA

Upstream 100 bases:

>100_bases
TTTTCCCGAAGTTTTCGATTCGTTGAAACTCCTTGACAAGGGAAACTTTCCTGTGTTCAATGATTGAATTCCTTCTACAA
AAAAATCCCAACACCCCTTT

Downstream 100 bases:

>100_bases
CAGAGGACTGAAGACGGACGAACTCGCTTCGCTCGTTCTAGACAGAGGACAGAAGCAGCAAAGCCGCTGGGCTTCCCTAC
GGGAAGCAATTGTAAAACGA

Product: Serine phosphatase RsbU, regulator of sigma subunit

Products: NA

Alternate protein names: Sigma factor sigB regulation protein rsbU [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MSERQLSLSLISNITAKINSTDNLEELLNIIIETTKDVLNTEGCSLLLYDRNEDCLVFNIAKGTKEKSLTELKVPRGKGI
AGMVLETLEPVIVNDAKNDPRIYRNIDETVGFVTYNLICVPMSTQGEIQGVLEAVNSLERKEFTNKDIKILQYLSDLAAI
AIRNRRLIRDLKSRANELDCLFQLSQAISNISEMDQFFNLTVNSVSEIMGAERVSLIFFNPKNGKYELKKSIGFSLKEEE
HFINEKEGVIRKVIETGKPILVQNTSSIMEDCVRPERYKTKSFISVPIRQDGKIIGILNSADKKSGNNFDNADQNVLSTI
SNQIAEAYNSLLSKEQKEKLNSIHRDMQIASQIQVNSLPNIPKKIQGLELETSYIASKEIGGDFYDLIYHNPDEVSVLIA
DVSGKGIAAALFMEFSKTIIAGEVSRNSSTSISLMSANRIIQEKSGYFMFVTVMLARINMAKRKICYSSAGHNEQLLYKA
KEKNVVSLSGKGMPLGIKESEIDEHELDYYPGDLLILYTDGVSEAMNESNEMYGLENLTKLIERNGDMPLGALKELIIDT
TDAFRGDADPHDDYTLFMIRLP

Sequences:

>Translated_582_residues
MSERQLSLSLISNITAKINSTDNLEELLNIIIETTKDVLNTEGCSLLLYDRNEDCLVFNIAKGTKEKSLTELKVPRGKGI
AGMVLETLEPVIVNDAKNDPRIYRNIDETVGFVTYNLICVPMSTQGEIQGVLEAVNSLERKEFTNKDIKILQYLSDLAAI
AIRNRRLIRDLKSRANELDCLFQLSQAISNISEMDQFFNLTVNSVSEIMGAERVSLIFFNPKNGKYELKKSIGFSLKEEE
HFINEKEGVIRKVIETGKPILVQNTSSIMEDCVRPERYKTKSFISVPIRQDGKIIGILNSADKKSGNNFDNADQNVLSTI
SNQIAEAYNSLLSKEQKEKLNSIHRDMQIASQIQVNSLPNIPKKIQGLELETSYIASKEIGGDFYDLIYHNPDEVSVLIA
DVSGKGIAAALFMEFSKTIIAGEVSRNSSTSISLMSANRIIQEKSGYFMFVTVMLARINMAKRKICYSSAGHNEQLLYKA
KEKNVVSLSGKGMPLGIKESEIDEHELDYYPGDLLILYTDGVSEAMNESNEMYGLENLTKLIERNGDMPLGALKELIIDT
TDAFRGDADPHDDYTLFMIRLP
>Mature_581_residues
SERQLSLSLISNITAKINSTDNLEELLNIIIETTKDVLNTEGCSLLLYDRNEDCLVFNIAKGTKEKSLTELKVPRGKGIA
GMVLETLEPVIVNDAKNDPRIYRNIDETVGFVTYNLICVPMSTQGEIQGVLEAVNSLERKEFTNKDIKILQYLSDLAAIA
IRNRRLIRDLKSRANELDCLFQLSQAISNISEMDQFFNLTVNSVSEIMGAERVSLIFFNPKNGKYELKKSIGFSLKEEEH
FINEKEGVIRKVIETGKPILVQNTSSIMEDCVRPERYKTKSFISVPIRQDGKIIGILNSADKKSGNNFDNADQNVLSTIS
NQIAEAYNSLLSKEQKEKLNSIHRDMQIASQIQVNSLPNIPKKIQGLELETSYIASKEIGGDFYDLIYHNPDEVSVLIAD
VSGKGIAAALFMEFSKTIIAGEVSRNSSTSISLMSANRIIQEKSGYFMFVTVMLARINMAKRKICYSSAGHNEQLLYKAK
EKNVVSLSGKGMPLGIKESEIDEHELDYYPGDLLILYTDGVSEAMNESNEMYGLENLTKLIERNGDMPLGALKELIIDTT
DAFRGDADPHDDYTLFMIRLP

Specific function: Positive regulator of sigma-B activity. Dephosphorylates rsbV in response to environmental stress conveyed from the rsbXST module [H]

COG id: COG2208

COG function: function code TK; Serine phosphatase RsbU, regulator of sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PP2C-like domain [H]

Homologues:

Organism=Homo sapiens, GI116536085, Length=357, Percent_Identity=25.2100840336134, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI4505657, Length=150, Percent_Identity=32, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI226246591, Length=150, Percent_Identity=32, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI219802706, Length=150, Percent_Identity=32, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI5729972, Length=169, Percent_Identity=26.0355029585799, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI194595488, Length=169, Percent_Identity=26.0355029585799, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI161076955, Length=356, Percent_Identity=22.752808988764, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI161076953, Length=356, Percent_Identity=22.752808988764, Blast_Score=72, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017944
- InterPro:   IPR001932
- InterPro:   IPR014787
- InterPro:   IPR010822
- ProDom:   PD685490 [H]

Pfam domain/function: PF08673 RsbU_N; PF07228 SpoIIE [H]

EC number: =3.1.3.3 [H]

Molecular weight: Translated: 65186; Mature: 65055

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERQLSLSLISNITAKINSTDNLEELLNIIIETTKDVLNTEGCSLLLYDRNEDCLVFNI
CCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
AKGTKEKSLTELKVPRGKGIAGMVLETLEPVIVNDAKNDPRIYRNIDETVGFVTYNLICV
CCCCCCCCHHHEECCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHEEEEEEEEE
PMSTQGEIQGVLEAVNSLERKEFTNKDIKILQYLSDLAAIAIRNRRLIRDLKSRANELDC
ECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFQLSQAISNISEMDQFFNLTVNSVSEIMGAERVSLIFFNPKNGKYELKKSIGFSLKEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHCCCCCCHHH
HFINEKEGVIRKVIETGKPILVQNTSSIMEDCVRPERYKTKSFISVPIRQDGKIIGILNS
HHCCHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEC
ADKKSGNNFDNADQNVLSTISNQIAEAYNSLLSKEQKEKLNSIHRDMQIASQIQVNSLPN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IPKKIQGLELETSYIASKEIGGDFYDLIYHNPDEVSVLIADVSGKGIAAALFMEFSKTII
CHHHHCCCEEEHHHHHHHHCCCCEEEHEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHE
AGEVSRNSSTSISLMSANRIIQEKSGYFMFVTVMLARINMAKRKICYSSAGHNEQLLYKA
EEECCCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
KEKNVVSLSGKGMPLGIKESEIDEHELDYYPGDLLILYTDGVSEAMNESNEMYGLENLTK
CCCCEEEECCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCHHHHHCCCCCCCCHHHHHH
LIERNGDMPLGALKELIIDTTDAFRGDADPHDDYTLFMIRLP
HHHCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEEEEECC
>Mature Secondary Structure 
SERQLSLSLISNITAKINSTDNLEELLNIIIETTKDVLNTEGCSLLLYDRNEDCLVFNI
CCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCEEEEEE
AKGTKEKSLTELKVPRGKGIAGMVLETLEPVIVNDAKNDPRIYRNIDETVGFVTYNLICV
CCCCCCCCHHHEECCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHEEEEEEEEE
PMSTQGEIQGVLEAVNSLERKEFTNKDIKILQYLSDLAAIAIRNRRLIRDLKSRANELDC
ECCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFQLSQAISNISEMDQFFNLTVNSVSEIMGAERVSLIFFNPKNGKYELKKSIGFSLKEEE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHCCCCCCHHH
HFINEKEGVIRKVIETGKPILVQNTSSIMEDCVRPERYKTKSFISVPIRQDGKIIGILNS
HHCCHHHHHHHHHHHCCCCEEEECHHHHHHHHCCCCCCCCCCEEEEEECCCCCEEEEEEC
ADKKSGNNFDNADQNVLSTISNQIAEAYNSLLSKEQKEKLNSIHRDMQIASQIQVNSLPN
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IPKKIQGLELETSYIASKEIGGDFYDLIYHNPDEVSVLIADVSGKGIAAALFMEFSKTII
CHHHHCCCEEEHHHHHHHHCCCCEEEHEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHE
AGEVSRNSSTSISLMSANRIIQEKSGYFMFVTVMLARINMAKRKICYSSAGHNEQLLYKA
EEECCCCCCCEEEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
KEKNVVSLSGKGMPLGIKESEIDEHELDYYPGDLLILYTDGVSEAMNESNEMYGLENLTK
CCCCEEEECCCCCCCCCCCCCCCCHHCCCCCCCEEEEEECCHHHHHCCCCCCCCHHHHHH
LIERNGDMPLGALKELIIDTTDAFRGDADPHDDYTLFMIRLP
HHHCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8002610; 8002609; 9384377; 8824586; 9786195; 12499568 [H]