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The map label for this gene is meaA [H]

Identifier: 116332504

GI number: 116332504

Start: 65997

End: 68009

Strand: Reverse

Name: meaA [H]

Synonym: LBJ_4062

Alternate gene names: 116332504

Gene position: 68009-65997 (Counterclockwise)

Preceding gene: 116332516

Following gene: 116332503

Centisome position: 22.69

GC content: 45.85

Gene sequence:

>2013_bases
ATGGAAAATAAAAATCATATCCTATACGACAAATCCGGCAAACCCTCTAAAGAACCTGCTTGGATTTTCAGAACGTATGC
GGGGCACACAAACGCGAGAGAATCTAACGAACTTTTTAGAAAAAATCTATCCAAAGGACAAACGGGTCTTTCCATCGCCT
TCGACTTGGCAACCCAGTGCGGTTATAGTTCCGATCATGCTATCGCAAAACCGGAAATCGGGAAGGTCGGCGTTCCGATC
AACACTCTGGAAGATTTTAGAATCCTTTTCAATCAAATTCCGATCGAAGAGATGAACACTTCGATGACGATCAACGGGAC
CTCGATGTATCTTTTGTCCCTTTACGTCGCCCTCGCCCAGGAACGCGGAGTAGACGTTGCCCTTCTTCAAGGAACCACTC
AGAACGACATCATTAAAGAATACCTCGCAAGGGGGACATACATCTTTCCTCCCGCACAATCGATCCGAGTCATCGTCGAT
ATGTACGAATATTGTCTGAAAAACATTCCGAAATGGAACCCTTCCAACATTTGTTCGTATCACCTGCAGGAAGCCGGAGC
GACACCCGTGCAGGAACTTGCGTTCGCACTCGCGACCGCAATGGCAATTCTCGACGCGATCAAGGAAAGAAATTGTTTCA
CCGCCGAAGAGTTCGAACAATGTGTGGGAAGAATTTCATTCTTCGTAAACGCGGGTATTCGTTTTGTAGAAGAGATGTGC
AAGATGCGCGCCTTTACGGAAATGTGGGACGAGATTACGAAAGATCGTTATCAAGTGAAACAGGAGAAATACCGCCGTTT
CCGTTACGGGGTTCAGGTGAATTCTCTCGGTCTCACCGAAGAACAACCAGAAAACAACGCATGGAGAATTCTCATAGAAG
CGTTAGGCGTCACCATGAGCCGCGATGCACGTTGCAGAGCGCTTCAGCTTCCCGCTTGGAACGAAGCTCTTTCCCTTCCA
AGACCTTGGGACCAACAATGGTCTTTGAGATTGCAGCAGGTTCTCGCATATGAAACTGATCTTCTCGAATATCCGGACCT
ATTCGAAGGTTCTAAAGTCGTCGAAAGTAAAGTAAAAGAACTCAAAGTAGAAGCTTACAAAGAAATTCAAAAGATACTTG
ATATGGGCGGAGCGGTCAAAGCAATCGAAAACGGTTATATGAAATCTCAGCTCGTCAAGTCCCAAGCGGAACGTCTTACT
AAGATCAACAACAATGAACTCATCATCGTTGGTAAAAACAAATGGACCGAGGGAACTCCTTCCCCACTCATGACCGATCA
AGACGGAGGAATTTTTAAAGTGGATCCTAAATCCGCAGAAGAAACCTTAGAAGTACTTGCAAAAGTAAAATCGACAAGAG
ACGCAAACAAAGTCAAAGAGGCTCTCTCTCAACTTGAAGCGGATGCGAAAGCCGGTAAAAATCTAATGTACGCTTCCATC
GAATGTGCAAAAACAGGAATTTCAACCGGCGAATGGGCGGACGTTCTCAGATCCGTCTTCGGAGAATATAGACCTGCCAC
CGGTGTAGAAGGACAAAAACTCAATCTTGAAACCGACAAAGTGATCCGAGTAAGGGGAAAAGTGGAAGCGTTTTTCAAAG
CGAACGGTTCCAGACCTAAAATCGTGGTCGGCAAACCGGGGCTAGACGGTCACTCTAACGGTGCGGAGATGATAGCCGTT
TCCGCAAAACACGCCGGATTTGACGTGATTTATTCCGGAATTCGTTTAACACCCGAGGAGATCGTTCAAACCGCGGTGGA
AGAAAATGCGGACGTGATCGGAGTTTCGATTCTTTCCGGTTCTCATTTAGAACTTGCGGAACAGATTTTCAACGAACTAA
AACACTACAAAGCGGATATCCCTGTGGTCTTTGGGGGAATCATCCCTTCGAACGACTTTGACTCTCTGACTAAACTCGGA
GTCAAAGCAATCTTCACTCCAAAAGACTACGATCTAATGGACGTGATGGAAAGAATCATCGATATCATTTCTCAGACGAT
AAAAGCCGCATAA

Upstream 100 bases:

>100_bases
TAGGGTTTTTGCGGAAATCAGAGCAAGGATAAAAATTTGGGTGAAATTCTACCAACACATTTTGAAGTATCAATGAGAAC
CTCGAATCGGGACAAAATTC

Downstream 100 bases:

>100_bases
CGCGGTTTGACGGGTATGCCGTGAAACATCGCAAAGAAGATCTCGCGAAACTCATCGAAGGAGCACGCGAAGGGGAAAAA
TTTCCCCTCGCGAAGCTCAT

Product: methylmalonyl-CoA mutase

Products: succinyl-CoA

Alternate protein names: NA

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MENKNHILYDKSGKPSKEPAWIFRTYAGHTNARESNELFRKNLSKGQTGLSIAFDLATQCGYSSDHAIAKPEIGKVGVPI
NTLEDFRILFNQIPIEEMNTSMTINGTSMYLLSLYVALAQERGVDVALLQGTTQNDIIKEYLARGTYIFPPAQSIRVIVD
MYEYCLKNIPKWNPSNICSYHLQEAGATPVQELAFALATAMAILDAIKERNCFTAEEFEQCVGRISFFVNAGIRFVEEMC
KMRAFTEMWDEITKDRYQVKQEKYRRFRYGVQVNSLGLTEEQPENNAWRILIEALGVTMSRDARCRALQLPAWNEALSLP
RPWDQQWSLRLQQVLAYETDLLEYPDLFEGSKVVESKVKELKVEAYKEIQKILDMGGAVKAIENGYMKSQLVKSQAERLT
KINNNELIIVGKNKWTEGTPSPLMTDQDGGIFKVDPKSAEETLEVLAKVKSTRDANKVKEALSQLEADAKAGKNLMYASI
ECAKTGISTGEWADVLRSVFGEYRPATGVEGQKLNLETDKVIRVRGKVEAFFKANGSRPKIVVGKPGLDGHSNGAEMIAV
SAKHAGFDVIYSGIRLTPEEIVQTAVEENADVIGVSILSGSHLELAEQIFNELKHYKADIPVVFGGIIPSNDFDSLTKLG
VKAIFTPKDYDLMDVMERIIDIISQTIKAA

Sequences:

>Translated_670_residues
MENKNHILYDKSGKPSKEPAWIFRTYAGHTNARESNELFRKNLSKGQTGLSIAFDLATQCGYSSDHAIAKPEIGKVGVPI
NTLEDFRILFNQIPIEEMNTSMTINGTSMYLLSLYVALAQERGVDVALLQGTTQNDIIKEYLARGTYIFPPAQSIRVIVD
MYEYCLKNIPKWNPSNICSYHLQEAGATPVQELAFALATAMAILDAIKERNCFTAEEFEQCVGRISFFVNAGIRFVEEMC
KMRAFTEMWDEITKDRYQVKQEKYRRFRYGVQVNSLGLTEEQPENNAWRILIEALGVTMSRDARCRALQLPAWNEALSLP
RPWDQQWSLRLQQVLAYETDLLEYPDLFEGSKVVESKVKELKVEAYKEIQKILDMGGAVKAIENGYMKSQLVKSQAERLT
KINNNELIIVGKNKWTEGTPSPLMTDQDGGIFKVDPKSAEETLEVLAKVKSTRDANKVKEALSQLEADAKAGKNLMYASI
ECAKTGISTGEWADVLRSVFGEYRPATGVEGQKLNLETDKVIRVRGKVEAFFKANGSRPKIVVGKPGLDGHSNGAEMIAV
SAKHAGFDVIYSGIRLTPEEIVQTAVEENADVIGVSILSGSHLELAEQIFNELKHYKADIPVVFGGIIPSNDFDSLTKLG
VKAIFTPKDYDLMDVMERIIDIISQTIKAA
>Mature_670_residues
MENKNHILYDKSGKPSKEPAWIFRTYAGHTNARESNELFRKNLSKGQTGLSIAFDLATQCGYSSDHAIAKPEIGKVGVPI
NTLEDFRILFNQIPIEEMNTSMTINGTSMYLLSLYVALAQERGVDVALLQGTTQNDIIKEYLARGTYIFPPAQSIRVIVD
MYEYCLKNIPKWNPSNICSYHLQEAGATPVQELAFALATAMAILDAIKERNCFTAEEFEQCVGRISFFVNAGIRFVEEMC
KMRAFTEMWDEITKDRYQVKQEKYRRFRYGVQVNSLGLTEEQPENNAWRILIEALGVTMSRDARCRALQLPAWNEALSLP
RPWDQQWSLRLQQVLAYETDLLEYPDLFEGSKVVESKVKELKVEAYKEIQKILDMGGAVKAIENGYMKSQLVKSQAERLT
KINNNELIIVGKNKWTEGTPSPLMTDQDGGIFKVDPKSAEETLEVLAKVKSTRDANKVKEALSQLEADAKAGKNLMYASI
ECAKTGISTGEWADVLRSVFGEYRPATGVEGQKLNLETDKVIRVRGKVEAFFKANGSRPKIVVGKPGLDGHSNGAEMIAV
SAKHAGFDVIYSGIRLTPEEIVQTAVEENADVIGVSILSGSHLELAEQIFNELKHYKADIPVVFGGIIPSNDFDSLTKLG
VKAIFTPKDYDLMDVMERIIDIISQTIKAA

Specific function: Required for the assimilation of methanol and ethanol [H]

COG id: COG1884

COG function: function code I; Methylmalonyl-CoA mutase, N-terminal domain/subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156105689, Length=634, Percent_Identity=34.384858044164, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI1789284, Length=657, Percent_Identity=35.4642313546423, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25144500, Length=635, Percent_Identity=35.748031496063, Blast_Score=388, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006159
- InterPro:   IPR016176
- InterPro:   IPR014348
- InterPro:   IPR006158
- InterPro:   IPR006099
- InterPro:   IPR006098 [H]

Pfam domain/function: PF02310 B12-binding; PF01642 MM_CoA_mutase [H]

EC number: 5.4.99.2

Molecular weight: Translated: 74938; Mature: 74938

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKNHILYDKSGKPSKEPAWIFRTYAGHTNARESNELFRKNLSKGQTGLSIAFDLATQC
CCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHC
GYSSDHAIAKPEIGKVGVPINTLEDFRILFNQIPIEEMNTSMTINGTSMYLLSLYVALAQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEECCHHHHHHHHHHHHHH
ERGVDVALLQGTTQNDIIKEYLARGTYIFPPAQSIRVIVDMYEYCLKNIPKWNPSNICSY
HCCCEEEEECCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
HLQEAGATPVQELAFALATAMAILDAIKERNCFTAEEFEQCVGRISFFVNAGIRFVEEMC
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KMRAFTEMWDEITKDRYQVKQEKYRRFRYGVQVNSLGLTEEQPENNAWRILIEALGVTMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RDARCRALQLPAWNEALSLPRPWDQQWSLRLQQVLAYETDLLEYPDLFEGSKVVESKVKE
CCCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LKVEAYKEIQKILDMGGAVKAIENGYMKSQLVKSQAERLTKINNNELIIVGKNKWTEGTP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
SPLMTDQDGGIFKVDPKSAEETLEVLAKVKSTRDANKVKEALSQLEADAKAGKNLMYASI
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEH
ECAKTGISTGEWADVLRSVFGEYRPATGVEGQKLNLETDKVIRVRGKVEAFFKANGSRPK
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCEEEEHHHEEEEEECCCCCCE
IVVGKPGLDGHSNGAEMIAVSAKHAGFDVIYSGIRLTPEEIVQTAVEENADVIGVSILSG
EEEECCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECC
SHLELAEQIFNELKHYKADIPVVFGGIIPSNDFDSLTKLGVKAIFTPKDYDLMDVMERII
CHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCEEEECCCCCCHHHHHHHHH
DIISQTIKAA
HHHHHHHHCC
>Mature Secondary Structure
MENKNHILYDKSGKPSKEPAWIFRTYAGHTNARESNELFRKNLSKGQTGLSIAFDLATQC
CCCCCCEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHC
GYSSDHAIAKPEIGKVGVPINTLEDFRILFNQIPIEEMNTSMTINGTSMYLLSLYVALAQ
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHCCCEEEECCHHHHHHHHHHHHHH
ERGVDVALLQGTTQNDIIKEYLARGTYIFPPAQSIRVIVDMYEYCLKNIPKWNPSNICSY
HCCCEEEEECCCCHHHHHHHHHHCCCEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
HLQEAGATPVQELAFALATAMAILDAIKERNCFTAEEFEQCVGRISFFVNAGIRFVEEMC
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
KMRAFTEMWDEITKDRYQVKQEKYRRFRYGVQVNSLGLTEEQPENNAWRILIEALGVTMS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHCCCCC
RDARCRALQLPAWNEALSLPRPWDQQWSLRLQQVLAYETDLLEYPDLFEGSKVVESKVKE
CCCCCEEEECCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
LKVEAYKEIQKILDMGGAVKAIENGYMKSQLVKSQAERLTKINNNELIIVGKNKWTEGTP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCC
SPLMTDQDGGIFKVDPKSAEETLEVLAKVKSTRDANKVKEALSQLEADAKAGKNLMYASI
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEEEEEH
ECAKTGISTGEWADVLRSVFGEYRPATGVEGQKLNLETDKVIRVRGKVEAFFKANGSRPK
HHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCEECCCCCCEEEEHHHEEEEEECCCCCCE
IVVGKPGLDGHSNGAEMIAVSAKHAGFDVIYSGIRLTPEEIVQTAVEENADVIGVSILSG
EEEECCCCCCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHHHHHHHCCCCEEEEEEECC
SHLELAEQIFNELKHYKADIPVVFGGIIPSNDFDSLTKLGVKAIFTPKDYDLMDVMERII
CHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHCCEEEECCCCCCHHHHHHHHH
DIISQTIKAA
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-methylmalonyl-CoA

Specific reaction: (R)-methylmalonyl-CoA = succinyl-CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8868443 [H]