Click here to switch to the map view.

The map label for this gene is hemL

Identifier: 116332457

GI number: 116332457

Start: 12461

End: 13786

Strand: Direct

Name: hemL

Synonym: LBJ_4011

Alternate gene names: 116332457

Gene position: 12461-13786 (Clockwise)

Preceding gene: 116332456

Following gene: 116332458

Centisome position: 4.16

GC content: 47.36

Gene sequence:

>1326_bases
TTGAGTCAACGTTCATCCGAATTGATTTCCGATTCATGGAAAGGTAGTTCTTCCGAAGAGCTTTTTGAAAGAGCTAAGAT
TGTTTCCCCCGGTGGAGTTCATTCTCCAGTCCGTTCCTTTCGTTCCGTGGGAGGCACTCCCGTATTTTTTGTCTCTGCGA
ACGGAGCCACGTTAACCGACGTTTCCGGTAAGGAATACGTCGATTTTTGTCTGAGTTTCGGCCCTTTGATTTTGGGGCAC
AGAGATCCGGAGGTGGAAGAAGTGGTTCGTGAAACGGCGGGGCTTGCATGGAGTTTCGGAACGGCGGAGCCCTACTCTCT
CGAACTTGCGGAATTTATCACGAATCGGATTCCTTGGGCCGAAAAAGTCCGTTTTGTGAATTCGGGTACCGAAGCGGTGA
TGAGTGCGCTTCGTGTCACTCGCGCGGCGACCGGAAGGGAAAAAATTTTCAAGTTTGACGGATGTTATCACGGTCATCTG
GATGCGCTTTTGGTCAAAGCGGGTTCCGGTCTTGCGGGAGAATCTTCTTCCGATAGCGCCGGGATTTCTTCGACTGCGAT
TGCAAATACTCTCGTATTGCCTTTGGACGATGAAATGGCCGTTCAAAAATTGTTCGAATCCGAAGGAAAAAATATCGCTG
CCTTGATTATCGAACCTCTACCGGCCAATTACGGACTTCTTGTACAAAGAAAGGAATTTCTTTTGAAAATCGTGGAGATC
GCGAAAAAATATGGAACGTTAGTCGTGTTTGATGAGGTGATTTCCGGATTTAGGACCGGTTTTCAGGGAATGTCCGGTTT
GCTCGGAATTCGTCCCGATTTAGTGACTTACGGCAAGATCATAGGGGGCGGTTTTCCCGTCGGTTGTTACGCTGGAAGAA
GGGATCTTTTGGATTTGGTCGCTCCTTCCGGACCGGTATATCAAGCCGGAACCTTAAGCGCCAATCCGTTCGGAATGAGG
GCGGGACTTGCAACTCTAAAAAAAGCCGAAAGGGATTCCATCTATTCGGTGTTAGAAGTTCGAACCAAAACTTTTGCCGA
CGAGATGGTAAAACTTCTGAATGGAAAAACGGATCAGGAATGGGAGGCCGTGACTCATTCTTCCCTATTCTGGTTTCGTA
AAAAGACACAACAAGCGGTGAGAAGGATCGATCAAATTCCGGAAGGTCATAAAGAAGGGTTTGCGGAAGTATTCCACGTT
TTGTTAAAGAACGGAATTTACCTCGCCCCTTCCGGTTACGAGGTAGGATTTTTATCCTGGGCCCATAATGATTCAGTGAT
CGCAAAAGTTTTGGAAATAGCGGATAAGGCCTTCAAAGAACTCTAA

Upstream 100 bases:

>100_bases
ATTTTAACGCGGCTTTGTGCGAGACCTGGCAGATTTTTTCTAGAGCGAGAGCCTCTTATTTAATAACTTATGGAGCAAGA
AAAGGTAAGGAGATTTTATC

Downstream 100 bases:

>100_bases
TGTCTTCTTTATGGAAAAATTCTAGAATCCTTTTTGCAATCGTTTGGTTGCTTGTCACGGTTTCACTCGGAGTTTGGTGG
TTTTTACTCGGGCTTAAGCT

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSQRSSELISDSWKGSSSEELFERAKIVSPGGVHSPVRSFRSVGGTPVFFVSANGATLTDVSGKEYVDFCLSFGPLILGH
RDPEVEEVVRETAGLAWSFGTAEPYSLELAEFITNRIPWAEKVRFVNSGTEAVMSALRVTRAATGREKIFKFDGCYHGHL
DALLVKAGSGLAGESSSDSAGISSTAIANTLVLPLDDEMAVQKLFESEGKNIAALIIEPLPANYGLLVQRKEFLLKIVEI
AKKYGTLVVFDEVISGFRTGFQGMSGLLGIRPDLVTYGKIIGGGFPVGCYAGRRDLLDLVAPSGPVYQAGTLSANPFGMR
AGLATLKKAERDSIYSVLEVRTKTFADEMVKLLNGKTDQEWEAVTHSSLFWFRKKTQQAVRRIDQIPEGHKEGFAEVFHV
LLKNGIYLAPSGYEVGFLSWAHNDSVIAKVLEIADKAFKEL

Sequences:

>Translated_441_residues
MSQRSSELISDSWKGSSSEELFERAKIVSPGGVHSPVRSFRSVGGTPVFFVSANGATLTDVSGKEYVDFCLSFGPLILGH
RDPEVEEVVRETAGLAWSFGTAEPYSLELAEFITNRIPWAEKVRFVNSGTEAVMSALRVTRAATGREKIFKFDGCYHGHL
DALLVKAGSGLAGESSSDSAGISSTAIANTLVLPLDDEMAVQKLFESEGKNIAALIIEPLPANYGLLVQRKEFLLKIVEI
AKKYGTLVVFDEVISGFRTGFQGMSGLLGIRPDLVTYGKIIGGGFPVGCYAGRRDLLDLVAPSGPVYQAGTLSANPFGMR
AGLATLKKAERDSIYSVLEVRTKTFADEMVKLLNGKTDQEWEAVTHSSLFWFRKKTQQAVRRIDQIPEGHKEGFAEVFHV
LLKNGIYLAPSGYEVGFLSWAHNDSVIAKVLEIADKAFKEL
>Mature_440_residues
SQRSSELISDSWKGSSSEELFERAKIVSPGGVHSPVRSFRSVGGTPVFFVSANGATLTDVSGKEYVDFCLSFGPLILGHR
DPEVEEVVRETAGLAWSFGTAEPYSLELAEFITNRIPWAEKVRFVNSGTEAVMSALRVTRAATGREKIFKFDGCYHGHLD
ALLVKAGSGLAGESSSDSAGISSTAIANTLVLPLDDEMAVQKLFESEGKNIAALIIEPLPANYGLLVQRKEFLLKIVEIA
KKYGTLVVFDEVISGFRTGFQGMSGLLGIRPDLVTYGKIIGGGFPVGCYAGRRDLLDLVAPSGPVYQAGTLSANPFGMRA
GLATLKKAERDSIYSVLEVRTKTFADEMVKLLNGKTDQEWEAVTHSSLFWFRKKTQQAVRRIDQIPEGHKEGFAEVFHVL
LKNGIYLAPSGYEVGFLSWAHNDSVIAKVLEIADKAFKEL

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=345, Percent_Identity=23.768115942029, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI284507298, Length=163, Percent_Identity=25.7668711656442, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786349, Length=428, Percent_Identity=40.8878504672897, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI1789016, Length=337, Percent_Identity=31.4540059347181, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787560, Length=300, Percent_Identity=33.6666666666667, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788044, Length=256, Percent_Identity=32.421875, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1789759, Length=322, Percent_Identity=26.3975155279503, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI145693181, Length=280, Percent_Identity=27.1428571428571, Blast_Score=88, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI25144271, Length=367, Percent_Identity=25.3405994550409, Blast_Score=91, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323470, Length=311, Percent_Identity=25.7234726688103, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324432, Length=254, Percent_Identity=28.3464566929134, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_LEPBJ (Q04NV4)

Other databases:

- EMBL:   CP000351
- RefSeq:   YP_802174.1
- ProteinModelPortal:   Q04NV4
- SMR:   Q04NV4
- STRING:   Q04NV4
- GeneID:   4412234
- GenomeReviews:   CP000351_GR
- KEGG:   lbj:LBJ_4011
- eggNOG:   COG0001
- HOGENOM:   HBG725944
- OMA:   VGCFGGK
- ProtClustDB:   PRK00062
- BioCyc:   LBOR355277:LBJ_4011-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 48001; Mature: 47870

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQRSSELISDSWKGSSSEELFERAKIVSPGGVHSPVRSFRSVGGTPVFFVSANGATLTD
CCCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEEC
VSGKEYVDFCLSFGPLILGHRDPEVEEVVRETAGLAWSFGTAEPYSLELAEFITNRIPWA
CCCHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCHH
EKVRFVNSGTEAVMSALRVTRAATGREKIFKFDGCYHGHLDALLVKAGSGLAGESSSDSA
HHHHHHCCCHHHHHHHHHHHHHHCCHHHHEEECCCCCCCHHHHHEECCCCCCCCCCCCCC
GISSTAIANTLVLPLDDEMAVQKLFESEGKNIAALIIEPLPANYGLLVQRKEFLLKIVEI
CCCHHHHHHEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHH
AKKYGTLVVFDEVISGFRTGFQGMSGLLGIRPDLVTYGKIIGGGFPVGCYAGRRDLLDLV
HHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHH
APSGPVYQAGTLSANPFGMRAGLATLKKAERDSIYSVLEVRTKTFADEMVKLLNGKTDQE
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
WEAVTHSSLFWFRKKTQQAVRRIDQIPEGHKEGFAEVFHVLLKNGIYLAPSGYEVGFLSW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCCCEEEEEE
AHNDSVIAKVLEIADKAFKEL
CCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SQRSSELISDSWKGSSSEELFERAKIVSPGGVHSPVRSFRSVGGTPVFFVSANGATLTD
CCCHHHHHHHCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCCEEEEEECCCCEEEC
VSGKEYVDFCLSFGPLILGHRDPEVEEVVRETAGLAWSFGTAEPYSLELAEFITNRIPWA
CCCHHHHHHHHHHCCEEECCCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCHH
EKVRFVNSGTEAVMSALRVTRAATGREKIFKFDGCYHGHLDALLVKAGSGLAGESSSDSA
HHHHHHCCCHHHHHHHHHHHHHHCCHHHHEEECCCCCCCHHHHHEECCCCCCCCCCCCCC
GISSTAIANTLVLPLDDEMAVQKLFESEGKNIAALIIEPLPANYGLLVQRKEFLLKIVEI
CCCHHHHHHEEEEECCHHHHHHHHHHHCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHH
AKKYGTLVVFDEVISGFRTGFQGMSGLLGIRPDLVTYGKIIGGGFPVGCYAGRRDLLDLV
HHHHCCEEEHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHCCHHHHHHHH
APSGPVYQAGTLSANPFGMRAGLATLKKAERDSIYSVLEVRTKTFADEMVKLLNGKTDQE
CCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
WEAVTHSSLFWFRKKTQQAVRRIDQIPEGHKEGFAEVFHVLLKNGIYLAPSGYEVGFLSW
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCEEECCCCCCEEEEEE
AHNDSVIAKVLEIADKAFKEL
CCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA