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The map label for this gene is hemB [H]

Identifier: 116332456

GI number: 116332456

Start: 11511

End: 12464

Strand: Direct

Name: hemB [H]

Synonym: LBJ_4010

Alternate gene names: 116332456

Gene position: 11511-12464 (Clockwise)

Preceding gene: 116332455

Following gene: 116332457

Centisome position: 3.84

GC content: 44.03

Gene sequence:

>954_bases
TTGGAAACCAAACAAAGAAGAATTCGCCTTAATGTAGGACTCAGAGATCTTGCGTCTTCGGAGAGTCTGAATTCAAAAAA
ACTAATACAACCTCTTTTTATCGCAGAAGGTTTAAAAGAAAAAGAGAAGATGGATTCTCTTCCGGGTGTTTACAGGGACA
CCGAGGTTTCCGTCTTAAAACAAGTAGAGTCGGATCTCAAAGCGGGAGTGACTCATTTTATTCTTTTTCTCGTTCCAAAG
TCCAAATCCAATTCTGAAATTCCGAAATCTTTTTACGAACGTTCCATCTCTTCCTTGAAAAAGGAATTTCCCGACGCGTT
TTTATGGATCGATACTTGTATGTGTTCACTCACGACTCACGGCCATTGTGGCCTTCTGCGTCCGGACGGAAGCGTCGATA
ACCCCTCTTCCGTAAAACATCTTTCCGAGATCGCTTTGACATATGCACAGTCCGGTGCGGATGGAATCGCTCCGAGTGAT
ATGATGGACGGAAGAATCAAAAGTCACCGTTCCGTTTTAGATAAAAACGGTTTTTCGAATATTCCAATTTTAAGTTATTC
TACTAAGTTTAAGAGCAATTTTTACGGGCCGTTTCGTGCGGCGGCGGATTCCGCTCCGGGGCACGGGGATCGTTCTTCCT
ACCAGATAGACGTTCGGAATCGAGAGGATTCGATTCTTTCCTCCGTGCGCGATAAAGAAGAAGGCGCCGATTTTTTAATG
GTCAAACCCGGGATGACTTCTATCGATCTTATCGGTCCGATTCGGGAAAAAACCGGACTTCCGGTAGGCGCTTATCAGGT
CAGCGGAGAATACGCGTCGATTCACTATCTTGCACAAAACGGTTTTTGTGATTTTAACGCGGCTTTGTGCGAGACCTGGC
AGATTTTTTCTAGAGCGAGAGCCTCTTATTTAATAACTTATGGAGCAAGAAAAGGTAAGGAGATTTTATCTTGA

Upstream 100 bases:

>100_bases
ATCCGAAAAGTTCTGGGAGAATCGGCGAACTTATCCGTTTTTGTACACTATGAGTCTTGGCTACAAAGTCTCGGACTTAA
AGAATTCAAAGGAAAAGAGC

Downstream 100 bases:

>100_bases
GTCAACGTTCATCCGAATTGATTTCCGATTCATGGAAAGGTAGTTCTTCCGAAGAGCTTTTTGAAAGAGCTAAGATTGTT
TCCCCCGGTGGAGTTCATTC

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
METKQRRIRLNVGLRDLASSESLNSKKLIQPLFIAEGLKEKEKMDSLPGVYRDTEVSVLKQVESDLKAGVTHFILFLVPK
SKSNSEIPKSFYERSISSLKKEFPDAFLWIDTCMCSLTTHGHCGLLRPDGSVDNPSSVKHLSEIALTYAQSGADGIAPSD
MMDGRIKSHRSVLDKNGFSNIPILSYSTKFKSNFYGPFRAAADSAPGHGDRSSYQIDVRNREDSILSSVRDKEEGADFLM
VKPGMTSIDLIGPIREKTGLPVGAYQVSGEYASIHYLAQNGFCDFNAALCETWQIFSRARASYLITYGARKGKEILS

Sequences:

>Translated_317_residues
METKQRRIRLNVGLRDLASSESLNSKKLIQPLFIAEGLKEKEKMDSLPGVYRDTEVSVLKQVESDLKAGVTHFILFLVPK
SKSNSEIPKSFYERSISSLKKEFPDAFLWIDTCMCSLTTHGHCGLLRPDGSVDNPSSVKHLSEIALTYAQSGADGIAPSD
MMDGRIKSHRSVLDKNGFSNIPILSYSTKFKSNFYGPFRAAADSAPGHGDRSSYQIDVRNREDSILSSVRDKEEGADFLM
VKPGMTSIDLIGPIREKTGLPVGAYQVSGEYASIHYLAQNGFCDFNAALCETWQIFSRARASYLITYGARKGKEILS
>Mature_317_residues
METKQRRIRLNVGLRDLASSESLNSKKLIQPLFIAEGLKEKEKMDSLPGVYRDTEVSVLKQVESDLKAGVTHFILFLVPK
SKSNSEIPKSFYERSISSLKKEFPDAFLWIDTCMCSLTTHGHCGLLRPDGSVDNPSSVKHLSEIALTYAQSGADGIAPSD
MMDGRIKSHRSVLDKNGFSNIPILSYSTKFKSNFYGPFRAAADSAPGHGDRSSYQIDVRNREDSILSSVRDKEEGADFLM
VKPGMTSIDLIGPIREKTGLPVGAYQVSGEYASIHYLAQNGFCDFNAALCETWQIFSRARASYLITYGARKGKEILS

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=299, Percent_Identity=38.7959866220736, Blast_Score=211, Evalue=7e-55,
Organism=Escherichia coli, GI87081728, Length=310, Percent_Identity=38.3870967741935, Blast_Score=211, Evalue=7e-56,
Organism=Saccharomyces cerevisiae, GI6321398, Length=306, Percent_Identity=38.8888888888889, Blast_Score=197, Evalue=2e-51,
Organism=Drosophila melanogaster, GI21358291, Length=315, Percent_Identity=37.1428571428571, Blast_Score=197, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 35110; Mature: 35110

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKQRRIRLNVGLRDLASSESLNSKKLIQPLFIAEGLKEKEKMDSLPGVYRDTEVSVLK
CCCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QVESDLKAGVTHFILFLVPKSKSNSEIPKSFYERSISSLKKEFPDAFLWIDTCMCSLTTH
HHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
GHCGLLRPDGSVDNPSSVKHLSEIALTYAQSGADGIAPSDMMDGRIKSHRSVLDKNGFSN
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
IPILSYSTKFKSNFYGPFRAAADSAPGHGDRSSYQIDVRNREDSILSSVRDKEEGADFLM
CCEEEECCHHCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEE
VKPGMTSIDLIGPIREKTGLPVGAYQVSGEYASIHYLAQNGFCDFNAALCETWQIFSRAR
ECCCCCEEHHHCCHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
ASYLITYGARKGKEILS
HHEEEEECCCCCHHHCC
>Mature Secondary Structure
METKQRRIRLNVGLRDLASSESLNSKKLIQPLFIAEGLKEKEKMDSLPGVYRDTEVSVLK
CCCCCCEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
QVESDLKAGVTHFILFLVPKSKSNSEIPKSFYERSISSLKKEFPDAFLWIDTCMCSLTTH
HHHHHHHHHHHHEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
GHCGLLRPDGSVDNPSSVKHLSEIALTYAQSGADGIAPSDMMDGRIKSHRSVLDKNGFSN
CCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
IPILSYSTKFKSNFYGPFRAAADSAPGHGDRSSYQIDVRNREDSILSSVRDKEEGADFLM
CCEEEECCHHCCCCCCCHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCCEEE
VKPGMTSIDLIGPIREKTGLPVGAYQVSGEYASIHYLAQNGFCDFNAALCETWQIFSRAR
ECCCCCEEHHHCCHHHHCCCCEEEEEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHH
ASYLITYGARKGKEILS
HHEEEEECCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]