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The map label for this gene is phoB [H]

Identifier: 116329429

GI number: 116329429

Start: 3446842

End: 3447546

Strand: Direct

Name: phoB [H]

Synonym: LBL_2897

Alternate gene names: 116329429

Gene position: 3446842-3447546 (Clockwise)

Preceding gene: 116329428

Following gene: 116329430

Centisome position: 95.36

GC content: 41.7

Gene sequence:

>705_bases
ATGAAAAACCAATCAGGGAACCCAGGGCAAAAAGTCCTCGTCGTAGATGATGAGGAAGATATCGCCGAACTGATCCGATT
TCACCTCGAAGAAAACGGCTACCAAGTAGATACGTGTCAGAACGGATTAGAAGTACTTCCAAAACTGGAAAAAAATACTC
CGGATCTAGTCATTTTAGATCTTATGCTTCCAGGAATAGGGGGTATGGATCTTTGTAAAAAAATCAAAGAAAAATATTCC
ATGCCGATCATTATGGTCACCGCCAAATCCGGTGAAACCGAAGCAGTTCTTGGACTCGAACTCGGCGCGGACGATTATGT
TCGCAAACCTTTCAGCACCAGGGAATTGATCGCAAGAGTGCGTTCCGTTTTAAGAAGAACGAAAGAAGGCGAGGAAGAAG
AACAATTCGAAGGAAATATTACCATCGGAAACATCTTTCTTAATCTGAAAGCACATAAAGCATTCATCAATAACTTCGAA
GTCGATTTAACGTTAATCGAGTATAAAATTCTGAATCTTTTCATGACCAACCCCGGCGTAGCTTTTACAAGGGACAAATT
ATTAGATCGGGTTTGGGGAAAAGATATTTACGTCACCGACAGAGCCGTCGACGTAAATATTAAAAGATTACGCGATAAAC
TCGGAGACGAAAAGGAAAGACTCGAAACGATACGCGGAATCGGCTATAGATTCAATGAGGCGTAG

Upstream 100 bases:

>100_bases
CGGTCGAAACAGAAGAAATCTGACGGTTTCTGATTTTCTAAAAAAGAAAAGTCGCTTTGTAAAATAATAATGATAGGCCG
ACAAAAAAGAACAGGACTTC

Downstream 100 bases:

>100_bases
CTTATTTTCAAAACTTCTTCTCAGTAACTGGTTACTTTTGCTAGTTCTTCTCGTTGTTGCGGGGACGGTTCTTTTTGCGG
AAAAGTTTGTTCATTCGGAT

Product: response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLVILDLMLPGIGGMDLCKKIKEKYS
MPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARVRSVLRRTKEGEEEEQFEGNITIGNIFLNLKAHKAFINNFE
VDLTLIEYKILNLFMTNPGVAFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA

Sequences:

>Translated_234_residues
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLVILDLMLPGIGGMDLCKKIKEKYS
MPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARVRSVLRRTKEGEEEEQFEGNITIGNIFLNLKAHKAFINNFE
VDLTLIEYKILNLFMTNPGVAFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA
>Mature_234_residues
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLVILDLMLPGIGGMDLCKKIKEKYS
MPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARVRSVLRRTKEGEEEEQFEGNITIGNIFLNLKAHKAFINNFE
VDLTLIEYKILNLFMTNPGVAFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=229, Percent_Identity=38.8646288209607, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1788394, Length=224, Percent_Identity=37.5, Blast_Score=160, Evalue=5e-41,
Organism=Escherichia coli, GI1790860, Length=228, Percent_Identity=40.7894736842105, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1789809, Length=227, Percent_Identity=37.4449339207048, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1786784, Length=222, Percent_Identity=35.5855855855856, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI2367329, Length=225, Percent_Identity=36.4444444444444, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1790552, Length=220, Percent_Identity=33.1818181818182, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=32.2869955156951, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=34.2105263157895, Blast_Score=124, Evalue=4e-30,
Organism=Escherichia coli, GI1789402, Length=224, Percent_Identity=33.9285714285714, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1790863, Length=239, Percent_Identity=31.3807531380753, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1787229, Length=224, Percent_Identity=32.1428571428571, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787375, Length=224, Percent_Identity=29.9107142857143, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI145693140, Length=238, Percent_Identity=29.8319327731092, Blast_Score=102, Evalue=2e-23,
Organism=Escherichia coli, GI1790299, Length=129, Percent_Identity=35.6589147286822, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788191, Length=120, Percent_Identity=30.8333333333333, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1788550, Length=107, Percent_Identity=30.8411214953271, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26691; Mature: 26691

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLVILD
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCEEEEE
LMLPGIGGMDLCKKIKEKYSMPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARV
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEECCCHHHHCCCCHHHHHHHH
RSVLRRTKEGEEEEQFEGNITIGNIFLNLKAHKAFINNFEVDLTLIEYKILNLFMTNPGV
HHHHHHHCCCCCHHHHCCCEEEEEEEEEEEHHHHHHCCCEEEEEEEHEEEEHHEECCCCC
AFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA
CHHHHHHHHHHCCCCEEEECCEEECHHHHHHHHHCCHHHHHHHHHHCCEEECCC
>Mature Secondary Structure
MKNQSGNPGQKVLVVDDEEDIAELIRFHLEENGYQVDTCQNGLEVLPKLEKNTPDLVILD
CCCCCCCCCCEEEEEECHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHCCCCCCEEEEE
LMLPGIGGMDLCKKIKEKYSMPIIMVTAKSGETEAVLGLELGADDYVRKPFSTRELIARV
ECCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCEEEEEEEECCCHHHHCCCCHHHHHHHH
RSVLRRTKEGEEEEQFEGNITIGNIFLNLKAHKAFINNFEVDLTLIEYKILNLFMTNPGV
HHHHHHHCCCCCHHHHCCCEEEEEEEEEEEHHHHHHCCCEEEEEEEHEEEEHHEECCCCC
AFTRDKLLDRVWGKDIYVTDRAVDVNIKRLRDKLGDEKERLETIRGIGYRFNEA
CHHHHHHHHHHCCCCEEEECCEEECHHHHHHHHHCCHHHHHHHHHHCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]