Click here to switch to the map view.

The map label for this gene is ispD

Identifier: 116329347

GI number: 116329347

Start: 3320410

End: 3321117

Strand: Direct

Name: ispD

Synonym: LBL_2796

Alternate gene names: 116329347

Gene position: 3320410-3321117 (Clockwise)

Preceding gene: 116329346

Following gene: 116329349

Centisome position: 91.86

GC content: 42.37

Gene sequence:

>708_bases
ATGAAGTCCCTATTTCTCTCTGAAAAAATTTATGTCCTGATTCTCGCCGGAGGAACCGGCACAAGGATGGGCTCGGAGAT
TCCAAAACAGTTTTTGGAATTTTCCAACGAGCCCATCTTGATTCACACACTCAAAAAATTCCAGAGCTGGAAAAAACAAA
AACAAATCGTTTTAGTTTCTCATCCGGAATTCATTTCAGAAACGGAATCTATTTGTAGCCCTTTTCTAGAAAACCAGGAT
TGTATCATAGAAGGCGGAGAGACAAGACACGGGTCCATGCTTCGGGGTTTATCCGCACTCACGATTCAAAGCGAAGACAT
CCTATTGATTCACGACGCCGCTCGACCGTTCGTTCTTTTAAAGGAATTGGATTTGTTATGCGAAAATATCCGTGAAAATG
GAATTTCCACCCTTGCTTCCCGCACTTCCGAAACCGTATTAGAAGAATCGAATGGAAAAACCTCGTCCTTCTTGGATCGG
GAACATATTTGGTTTATGAAAACTCCGCAAGGAATCCGAGGAGATATTTTGAAAGAACTGTTAACATTGCCGATGGACCC
GATTCCCACCGATCTTTGCTCTTGGGCTTTAACGGCAGGAAAAAAATCTTCCATCGTAGAGTCGCATCCCTTTAACCTTA
AGATTACCCGTAAGGAAGACTTAGAACTGGCGGAATTTTATTCGGATTTGTTTGAGAAGTTACGTTAG

Upstream 100 bases:

>100_bases
AATTACAATTCTTTTCCCGAGACTGCGGAAGTTCTTGTGGACTTAGACGGAAGTTTTCAACTCATTCGGAAACGTCAAAA
TCTAGAGCAAATTTTTCAGA

Downstream 100 bases:

>100_bases
AATCTCTTGATGTTTGAGACTCGTCAATTTTGCGACCCAAGAAAAATCCCGATCTTTGCGGGCACCGTAAGTCTATTTCG
AATCGCATAAACTGCAATTT

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MKSLFLSEKIYVLILAGGTGTRMGSEIPKQFLEFSNEPILIHTLKKFQSWKKQKQIVLVSHPEFISETESICSPFLENQD
CIIEGGETRHGSMLRGLSALTIQSEDILLIHDAARPFVLLKELDLLCENIRENGISTLASRTSETVLEESNGKTSSFLDR
EHIWFMKTPQGIRGDILKELLTLPMDPIPTDLCSWALTAGKKSSIVESHPFNLKITRKEDLELAEFYSDLFEKLR

Sequences:

>Translated_235_residues
MKSLFLSEKIYVLILAGGTGTRMGSEIPKQFLEFSNEPILIHTLKKFQSWKKQKQIVLVSHPEFISETESICSPFLENQD
CIIEGGETRHGSMLRGLSALTIQSEDILLIHDAARPFVLLKELDLLCENIRENGISTLASRTSETVLEESNGKTSSFLDR
EHIWFMKTPQGIRGDILKELLTLPMDPIPTDLCSWALTAGKKSSIVESHPFNLKITRKEDLELAEFYSDLFEKLR
>Mature_235_residues
MKSLFLSEKIYVLILAGGTGTRMGSEIPKQFLEFSNEPILIHTLKKFQSWKKQKQIVLVSHPEFISETESICSPFLENQD
CIIEGGETRHGSMLRGLSALTIQSEDILLIHDAARPFVLLKELDLLCENIRENGISTLASRTSETVLEESNGKTSSFLDR
EHIWFMKTPQGIRGDILKELLTLPMDPIPTDLCSWALTAGKKSSIVESHPFNLKITRKEDLELAEFYSDLFEKLR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Homo sapiens, GI157412259, Length=230, Percent_Identity=28.695652173913, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI157671913, Length=118, Percent_Identity=32.2033898305085, Blast_Score=69, Evalue=4e-12,
Organism=Escherichia coli, GI1789104, Length=224, Percent_Identity=31.25, Blast_Score=97, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_LEPBJ (Q04VR0)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799768.1
- ProteinModelPortal:   Q04VR0
- SMR:   Q04VR0
- STRING:   Q04VR0
- GeneID:   4409941
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0280
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   REQQDFW
- PhylomeDB:   Q04VR0
- ProtClustDB:   CLSK573749
- BioCyc:   LBOR355277:LBJ_0280-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 26639; Mature: 26639

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLFLSEKIYVLILAGGTGTRMGSEIPKQFLEFSNEPILIHTLKKFQSWKKQKQIVLVS
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCEEEEE
HPEFISETESICSPFLENQDCIIEGGETRHGSMLRGLSALTIQSEDILLIHDAARPFVLL
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCHHEEECCCCEEEEECCCCHHHHH
KELDLLCENIRENGISTLASRTSETVLEESNGKTSSFLDREHIWFMKTPQGIRGDILKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHH
LTLPMDPIPTDLCSWALTAGKKSSIVESHPFNLKITRKEDLELAEFYSDLFEKLR
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSLFLSEKIYVLILAGGTGTRMGSEIPKQFLEFSNEPILIHTLKKFQSWKKQKQIVLVS
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHCCCCCEEEHHHHHHHHHHHHCCEEEEE
HPEFISETESICSPFLENQDCIIEGGETRHGSMLRGLSALTIQSEDILLIHDAARPFVLL
CCHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCHHEEECCCCEEEEECCCCHHHHH
KELDLLCENIRENGISTLASRTSETVLEESNGKTSSFLDREHIWFMKTPQGIRGDILKEL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCCHHHHHHHH
LTLPMDPIPTDLCSWALTAGKKSSIVESHPFNLKITRKEDLELAEFYSDLFEKLR
HHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA