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The map label for this gene is leuA-4 [H]

Identifier: 116329287

GI number: 116329287

Start: 3237277

End: 3238173

Strand: Direct

Name: leuA-4 [H]

Synonym: LBL_2733

Alternate gene names: 116329287

Gene position: 3237277-3238173 (Clockwise)

Preceding gene: 116329286

Following gene: 116329288

Centisome position: 89.56

GC content: 42.03

Gene sequence:

>897_bases
ATGGAAATAAAAATCACGGAAGTCGGTCCGCGTGATGGACTTCAAAACGAAAAACGTGCGGTATCAACAGAGATCAAAGC
GGAATACATTCGACGTTTGGTTCAAGCGGGTTTAAAAAACATAGAAGCGACGTCTTTTGTAAAAAAAGAATCCATTCCTC
AATTGGCGGATGCTTCAGAACTTTCCGCACTTTTGGATCTGAACGGACATATTCATTATTCAGCACTGACTCCGAATATA
AGAGGTTATGAAGCCGCGAAAAACGCTGGTTATAAGGAAGTTGCGGTTTTTACGGCGGCTTCGGAATCATTCACAAAAAA
AAATATCAATCGAACAATCACGGAATCCATCGACGGATTTAAAGAAATTTTCAAACTTGCGAAAGACGACGGTATCCGAG
TTCGCGGTTATGTTTCCACCGTGATCGATTGTCCTTATGAAGGTAAAATCGATCCGAATCAGGTTTTCAACGTTTCCAAA
ATTCTTTTGGATTTAGGCGCTTACGAAATTTCTCTCGGCGAAACCATCGGCACGGGAGTTCCCGCAGAGGTAGAAGTATT
ATTAGAACGCCTTCTCAAAGAAATTCCTGCAAATAAACTTGCAGGACACTTCCATGATACGTACGGAATGGCGATCGCAA
ACGTGGAAAAAGCTTTTTCAATGGGACTTCGCTCCTTCGACTCTTCTTCAGGAGGTCTGGGAGGATGTCCTTACGCCAAA
GGAGCTGCCGGAAATTTAGCTACTGACGACTTGGTATATTTTCTAGAAAAATCAGGAATCTCTACGGGAATCGACCCTGA
CCTTTTATGGGAAGCGTCCGCTTTTATGGAAAAAGCTCTTTTCAGAGAATTACAGTCAAGAACTTATCTGGCAAATAAAA
AGAAAAGAGAATCTTGA

Upstream 100 bases:

>100_bases
TTTGAGTCGAAATTGAAAAGGATCCATAAAACATAGCAATAGACAAGAACCAGGATATTTAGCTTGCCGACTTTCCATAC
AAAGATCGGATAATAAGAAA

Downstream 100 bases:

>100_bases
AACATTCTTCTCAAAAACTCAAACAGGTTCTGAAAAGAATTTTTTTAGAATACCAGACACCCCAGTATCTGGGGTCGGAT
CCGATAGAATTTCCCCATTC

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MEIKITEVGPRDGLQNEKRAVSTEIKAEYIRRLVQAGLKNIEATSFVKKESIPQLADASELSALLDLNGHIHYSALTPNI
RGYEAAKNAGYKEVAVFTAASESFTKKNINRTITESIDGFKEIFKLAKDDGIRVRGYVSTVIDCPYEGKIDPNQVFNVSK
ILLDLGAYEISLGETIGTGVPAEVEVLLERLLKEIPANKLAGHFHDTYGMAIANVEKAFSMGLRSFDSSSGGLGGCPYAK
GAAGNLATDDLVYFLEKSGISTGIDPDLLWEASAFMEKALFRELQSRTYLANKKKRES

Sequences:

>Translated_298_residues
MEIKITEVGPRDGLQNEKRAVSTEIKAEYIRRLVQAGLKNIEATSFVKKESIPQLADASELSALLDLNGHIHYSALTPNI
RGYEAAKNAGYKEVAVFTAASESFTKKNINRTITESIDGFKEIFKLAKDDGIRVRGYVSTVIDCPYEGKIDPNQVFNVSK
ILLDLGAYEISLGETIGTGVPAEVEVLLERLLKEIPANKLAGHFHDTYGMAIANVEKAFSMGLRSFDSSSGGLGGCPYAK
GAAGNLATDDLVYFLEKSGISTGIDPDLLWEASAFMEKALFRELQSRTYLANKKKRES
>Mature_298_residues
MEIKITEVGPRDGLQNEKRAVSTEIKAEYIRRLVQAGLKNIEATSFVKKESIPQLADASELSALLDLNGHIHYSALTPNI
RGYEAAKNAGYKEVAVFTAASESFTKKNINRTITESIDGFKEIFKLAKDDGIRVRGYVSTVIDCPYEGKIDPNQVFNVSK
ILLDLGAYEISLGETIGTGVPAEVEVLLERLLKEIPANKLAGHFHDTYGMAIANVEKAFSMGLRSFDSSSGGLGGCPYAK
GAAGNLATDDLVYFLEKSGISTGIDPDLLWEASAFMEKALFRELQSRTYLANKKKRES

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=292, Percent_Identity=52.0547945205479, Blast_Score=297, Evalue=1e-80,
Organism=Homo sapiens, GI109150422, Length=290, Percent_Identity=50.6896551724138, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI109150427, Length=290, Percent_Identity=50.6896551724138, Blast_Score=296, Evalue=2e-80,
Organism=Homo sapiens, GI260654708, Length=137, Percent_Identity=50.3649635036496, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI17510563, Length=294, Percent_Identity=46.9387755102041, Blast_Score=268, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24582381, Length=293, Percent_Identity=47.7815699658703, Blast_Score=285, Evalue=4e-77,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 32581; Mature: 32581

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIKITEVGPRDGLQNEKRAVSTEIKAEYIRRLVQAGLKNIEATSFVKKESIPQLADASE
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHH
LSALLDLNGHIHYSALTPNIRGYEAAKNAGYKEVAVFTAASESFTKKNINRTITESIDGF
HHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHH
KEIFKLAKDDGIRVRGYVSTVIDCPYEGKIDPNQVFNVSKILLDLGAYEISLGETIGTGV
HHHHHHHCCCCEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHCCCC
PAEVEVLLERLLKEIPANKLAGHFHDTYGMAIANVEKAFSMGLRSFDSSSGGLGGCPYAK
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GAAGNLATDDLVYFLEKSGISTGIDPDLLWEASAFMEKALFRELQSRTYLANKKKRES
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MEIKITEVGPRDGLQNEKRAVSTEIKAEYIRRLVQAGLKNIEATSFVKKESIPQLADASE
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHH
LSALLDLNGHIHYSALTPNIRGYEAAKNAGYKEVAVFTAASESFTKKNINRTITESIDGF
HHHHHHCCCCEEEEEECCCCCCCHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHH
KEIFKLAKDDGIRVRGYVSTVIDCPYEGKIDPNQVFNVSKILLDLGAYEISLGETIGTGV
HHHHHHHCCCCEEEEEEHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEECCCHHCCCC
PAEVEVLLERLLKEIPANKLAGHFHDTYGMAIANVEKAFSMGLRSFDSSSGGLGGCPYAK
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
GAAGNLATDDLVYFLEKSGISTGIDPDLLWEASAFMEKALFRELQSRTYLANKKKRES
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]