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The map label for this gene is rlmL [H]

Identifier: 116329277

GI number: 116329277

Start: 3221909

End: 3222916

Strand: Direct

Name: rlmL [H]

Synonym: LBL_2720

Alternate gene names: 116329277

Gene position: 3221909-3222916 (Clockwise)

Preceding gene: 116329275

Following gene: 116329279

Centisome position: 89.14

GC content: 39.38

Gene sequence:

>1008_bases
ATGTCGGAAAAACGATCCGAGAAATTCAATTCGCTTAACGATTTCGGAAGTCCAATGGCAATGTTTCGCAACAGACTTTT
AAGAATGTCCAAACATTGGAAGAAGTGGGCGCGAAAAAGGGGTATAGAATGTTTTCGAATTTACGACCGAGATATACCTC
AGGTTCCCGTAAGCGTGGATCTTTACGGACCGTACTGTCAAATCTCGGCGTATAAGAACAGTTATGAAATTTCGGATGAA
ACAAGAGAAAGGGAGAACGTGGAAATCAGACAGATCGTCGCGGAAACGTTGAATCTTGACGCGGGTTTTATATTTTGGAA
AAAGAGAGAACCTAAAAAAGGCAAAAAACAATACGAGAAACAATCCGAACAGTCGAAGTTGCTAGAAGTAACAGAGAACG
GCCTCCGATTCTACATAAACTTATCCGATTATATCGATACTGGATTATTCTTAGATCATAGAATCACAAGGGATCTTATT
CGCAAAGAATCAAAGGGAAAAAAATTCCTAAATCTTTTTTCTTATACGGGATCGTTTACCGTTTACGCGGCGTCAGGTGG
AGCGACCCAAAGTTTAAGCATAGATCTTTCCAATACATACTTGGCTTGGGCTGAAGAAAATTTAAAGTTAAACGGACTCT
CTCTTATAAAACATCGTCTACTCAGAGCGGATGTGGTGGAATGGCTTTATAACGAAAGAAAAAAGCCGGATCGGGAAAGA
TATGATCTGATTGTTCTCGATCCACCGACTTTTTCCAATAGCAAAAAAATGACGGGCATCTTCGACATACAAAAAGATCA
TGTGGAAATTTTAAACATTCTCTATCGAGACTTTGCTCTCCCCGGAGCCGTATTGTATTTTTCCACGAACTTTAGAAAGT
TCGAACTTTCCCAAACGTCCGTTCTCTGGAACAATCCGAAAGACATTACAAAAATGACCCTTCCAGACGATTTCAGAAAT
CAAAAAATTCATTTTTGCTGGAAAATGGAAAAACCGAATCGTTCTTAA

Upstream 100 bases:

>100_bases
CCTGCTATCGTTGTTTTTAGCGACAAATTTTCCAGTCTTTTTACGGAATCTCGACTTCGTTGTGATTGGATTTTAACCGA
GATTTTTGTACATTGGCTCC

Downstream 100 bases:

>100_bases
AGTTTTGGTACGGTTCTTATTCTAAAATCGAATTTTTGAAGAAACACAAAATTGCATAATTCAAGCGTCATAACTATTCT
AACGTGAGTTCGCATAACAA

Product: SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSEKRSEKFNSLNDFGSPMAMFRNRLLRMSKHWKKWARKRGIECFRIYDRDIPQVPVSVDLYGPYCQISAYKNSYEISDE
TRERENVEIRQIVAETLNLDAGFIFWKKREPKKGKKQYEKQSEQSKLLEVTENGLRFYINLSDYIDTGLFLDHRITRDLI
RKESKGKKFLNLFSYTGSFTVYAASGGATQSLSIDLSNTYLAWAEENLKLNGLSLIKHRLLRADVVEWLYNERKKPDRER
YDLIVLDPPTFSNSKKMTGIFDIQKDHVEILNILYRDFALPGAVLYFSTNFRKFELSQTSVLWNNPKDITKMTLPDDFRN
QKIHFCWKMEKPNRS

Sequences:

>Translated_335_residues
MSEKRSEKFNSLNDFGSPMAMFRNRLLRMSKHWKKWARKRGIECFRIYDRDIPQVPVSVDLYGPYCQISAYKNSYEISDE
TRERENVEIRQIVAETLNLDAGFIFWKKREPKKGKKQYEKQSEQSKLLEVTENGLRFYINLSDYIDTGLFLDHRITRDLI
RKESKGKKFLNLFSYTGSFTVYAASGGATQSLSIDLSNTYLAWAEENLKLNGLSLIKHRLLRADVVEWLYNERKKPDRER
YDLIVLDPPTFSNSKKMTGIFDIQKDHVEILNILYRDFALPGAVLYFSTNFRKFELSQTSVLWNNPKDITKMTLPDDFRN
QKIHFCWKMEKPNRS
>Mature_334_residues
SEKRSEKFNSLNDFGSPMAMFRNRLLRMSKHWKKWARKRGIECFRIYDRDIPQVPVSVDLYGPYCQISAYKNSYEISDET
RERENVEIRQIVAETLNLDAGFIFWKKREPKKGKKQYEKQSEQSKLLEVTENGLRFYINLSDYIDTGLFLDHRITRDLIR
KESKGKKFLNLFSYTGSFTVYAASGGATQSLSIDLSNTYLAWAEENLKLNGLSLIKHRLLRADVVEWLYNERKKPDRERY
DLIVLDPPTFSNSKKMTGIFDIQKDHVEILNILYRDFALPGAVLYFSTNFRKFELSQTSVLWNNPKDITKMTLPDDFRNQ
KIHFCWKMEKPNRS

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=309, Percent_Identity=44.336569579288, Blast_Score=264, Evalue=8e-72,
Organism=Escherichia coli, GI145693111, Length=258, Percent_Identity=28.2945736434109, Blast_Score=104, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 39617; Mature: 39486

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKRSEKFNSLNDFGSPMAMFRNRLLRMSKHWKKWARKRGIECFRIYDRDIPQVPVSVD
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEE
LYGPYCQISAYKNSYEISDETRERENVEIRQIVAETLNLDAGFIFWKKREPKKGKKQYEK
ECCCEEEEEECCCCEECCCHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
QSEQSKLLEVTENGLRFYINLSDYIDTGLFLDHRITRDLIRKESKGKKFLNLFSYTGSFT
HHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
VYAASGGATQSLSIDLSNTYLAWAEENLKLNGLSLIKHRLLRADVVEWLYNERKKPDRER
EEEECCCCCEEEEEEECCCEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YDLIVLDPPTFSNSKKMTGIFDIQKDHVEILNILYRDFALPGAVLYFSTNFRKFELSQTS
CCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCE
VLWNNPKDITKMTLPDDFRNQKIHFCWKMEKPNRS
EEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
SEKRSEKFNSLNDFGSPMAMFRNRLLRMSKHWKKWARKRGIECFRIYDRDIPQVPVSVD
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEE
LYGPYCQISAYKNSYEISDETRERENVEIRQIVAETLNLDAGFIFWKKREPKKGKKQYEK
ECCCEEEEEECCCCEECCCHHHHHCCHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
QSEQSKLLEVTENGLRFYINLSDYIDTGLFLDHRITRDLIRKESKGKKFLNLFSYTGSFT
HHHHHHHHHHCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEE
VYAASGGATQSLSIDLSNTYLAWAEENLKLNGLSLIKHRLLRADVVEWLYNERKKPDRER
EEEECCCCCEEEEEEECCCEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YDLIVLDPPTFSNSKKMTGIFDIQKDHVEILNILYRDFALPGAVLYFSTNFRKFELSQTS
CCEEEECCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCEEEEECCCE
VLWNNPKDITKMTLPDDFRNQKIHFCWKMEKPNRS
EEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA