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The map label for this gene is nuoB

Identifier: 116329153

GI number: 116329153

Start: 3055641

End: 3056201

Strand: Reverse

Name: nuoB

Synonym: LBL_2577

Alternate gene names: 116329153

Gene position: 3056201-3055641 (Counterclockwise)

Preceding gene: 116329154

Following gene: 116329152

Centisome position: 84.56

GC content: 51.16

Gene sequence:

>561_bases
ATGGGATTGAGTGATTTGAGTAAAGCTCCGGGCCAAGCCCTGGGAGATATGCTCCAGCTCGGAAACATCGAATCCGTGAT
TCAATGGGGAAGGGGTAACTCCCTTTGGCCTTTTCCTTTCGCTACTGCTTGTTGCGGAATCGAGTATATGAGTACTGCCT
GTTCCGATTACGACATTGCCCGTTTCGGGGCGGAGCGTCCTTCGTTTTCTCCGCGTCAGGCGGACATGATCCTAGTATTG
GGAACGATCACATACAAGATGGCTCCCGTTCTTCGACAGATTTATGATCAAATGGCAGAGCCCAAGTTCGTCATCAGCGT
CGGGGCTTGTGCTTCCTCCGGGGGAATGTTCAACACCTACGGAGTTTTGCAGGGAGTGGACCGAATACTTCCGGTGGACA
TATATGTTCCCGGTTGTCCGCCGAGGCCTGAGGCGATTTTAGACGCACTCGTTAAATTGCAGACGAAACTGAAAACCCAG
GGGTTGGAAGCGAGACGCCAGGAAGTGATGCAGAAAATCCAGGAATTGAATGAAAGAAATAAACCCCTGGTCGTACGATG
A

Upstream 100 bases:

>100_bases
AAAACGCGGGACTCGGTTTTTTTCTCGTCTTCGAGATGTTTGTGTTTGTCCTGACCTTAGTTGTGGGTTTATACTATATC
TGGAAGAAAGGAGCGCTGGA

Downstream 100 bases:

>100_bases
AAGAAGAAGTAGAACGTTTTCTTAAAGAAAAATTTCCGCATTTTTTGGACGTACAAGAACCGGTCATTTCCAACGTTCCG
GTTTTTTATCTAAAGGCCGA

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MGLSDLSKAPGQALGDMLQLGNIESVIQWGRGNSLWPFPFATACCGIEYMSTACSDYDIARFGAERPSFSPRQADMILVL
GTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFNTYGVLQGVDRILPVDIYVPGCPPRPEAILDALVKLQTKLKTQ
GLEARRQEVMQKIQELNERNKPLVVR

Sequences:

>Translated_186_residues
MGLSDLSKAPGQALGDMLQLGNIESVIQWGRGNSLWPFPFATACCGIEYMSTACSDYDIARFGAERPSFSPRQADMILVL
GTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFNTYGVLQGVDRILPVDIYVPGCPPRPEAILDALVKLQTKLKTQ
GLEARRQEVMQKIQELNERNKPLVVR
>Mature_185_residues
GLSDLSKAPGQALGDMLQLGNIESVIQWGRGNSLWPFPFATACCGIEYMSTACSDYDIARFGAERPSFSPRQADMILVLG
TITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFNTYGVLQGVDRILPVDIYVPGCPPRPEAILDALVKLQTKLKTQG
LEARRQEVMQKIQELNERNKPLVVR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=48.5915492957746, Blast_Score=158, Evalue=3e-39,
Organism=Escherichia coli, GI1788624, Length=146, Percent_Identity=51.3698630136986, Blast_Score=177, Evalue=5e-46,
Organism=Escherichia coli, GI1789074, Length=123, Percent_Identity=43.0894308943089, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1788834, Length=120, Percent_Identity=40, Blast_Score=80, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17509685, Length=142, Percent_Identity=50.7042253521127, Blast_Score=152, Evalue=9e-38,
Organism=Drosophila melanogaster, GI18859983, Length=140, Percent_Identity=51.4285714285714, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24642371, Length=140, Percent_Identity=51.4285714285714, Blast_Score=161, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24651058, Length=137, Percent_Identity=51.8248175182482, Blast_Score=156, Evalue=6e-39,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_LEPBJ (Q04V71)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_799957.1
- ProteinModelPortal:   Q04V71
- SMR:   Q04V71
- STRING:   Q04V71
- GeneID:   4409401
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_0502
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   CCAIEFM
- PhylomeDB:   Q04V71
- ProtClustDB:   PRK14814
- BioCyc:   LBOR355277:LBJ_0502-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20458; Mature: 20327

Theoretical pI: Translated: 7.80; Mature: 7.80

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
7.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLSDLSKAPGQALGDMLQLGNIESVIQWGRGNSLWPFPFATACCGIEYMSTACSDYDIA
CCCCHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
RFGAERPSFSPRQADMILVLGTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFNTY
HHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHH
GVLQGVDRILPVDIYVPGCPPRPEAILDALVKLQTKLKTQGLEARRQEVMQKIQELNERN
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
KPLVVR
CCEECC
>Mature Secondary Structure 
GLSDLSKAPGQALGDMLQLGNIESVIQWGRGNSLWPFPFATACCGIEYMSTACSDYDIA
CCCHHHCCCHHHHHHHHHHCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHH
RFGAERPSFSPRQADMILVLGTITYKMAPVLRQIYDQMAEPKFVISVGACASSGGMFNTY
HHCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHH
GVLQGVDRILPVDIYVPGCPPRPEAILDALVKLQTKLKTQGLEARRQEVMQKIQELNERN
HHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCC
KPLVVR
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA