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The map label for this gene is nuoN [H]

Identifier: 116329143

GI number: 116329143

Start: 3044881

End: 3046359

Strand: Reverse

Name: nuoN [H]

Synonym: LBL_2567

Alternate gene names: 116329143

Gene position: 3046359-3044881 (Counterclockwise)

Preceding gene: 116329144

Following gene: 116329140

Centisome position: 84.28

GC content: 44.83

Gene sequence:

>1479_bases
ATGAATTTTATCCCGGATGGTTTCGATTTATTTTCTATTCTTCCTGCATTGATTTTGGCAACAGGAGGAGTGTTTCTGAT
TTGTCTTTCCGTACTTTTTAAAACGAAAGAATTTCTAATCGTACGATACGTGTCCGGACTGGTTCTCCTCGTCGCGTTCG
GGAGCGTGTTTTACGTCGCCAAGTTTCAATATCCTGGAAACGGAGGAACCTATTTCGGCGGTCAGATCGAAAACTCCGTA
TTGTCTTTTTGGTTAAATCTAATTTATCTTTCGATGGCCATCGGAACCGCGGCAGTAGCTCCTAGAATATTAAAAAATCA
TAAAATAGAATTTCCGGAGTTCTATCCTTTGTTATTGTTTGCGACCTGCGGAATGATACTGATGACGTCGGGACAGGACT
TTATTCTCGTATTTGTCGCACTCGAACTGATGAGCATTTGCTTATACATTCTGATCGGAATGGCGAGAAGCGATTTGTTT
TCCTTGGAAGCGACCTTGAAATATTTTCTCTTGGGGAGTTTTTCCTCCGGGTTTATGCTGATGGGAATCGCATTTCTATT
TGGCGGAAGCGGGTCGACCAACATAACGGCGGCGTTAAAGCCATTGGTGATCGCGGGGTATCAAGGAAATTTTACAAAGA
TCGGGCTCGTTTTGTTCATCACGGGAGTCGCGTTTAAGATCGCGCTTTTTCCGTATCACGCTTGGACTCCGGACGCGTAC
GAAGGAGCGCTGACGCCCGTAACGGGTTATATGTCCACCGCCGCAAAGGCCGCGTCGATCGGGCTTCTTCTCGTGCTTTA
TACAAAACTTCCTCTGCCTCTCCAAGACAGTACTTGGGCGTGGTTGCCCGGAATTCTTGCATTGTTATCGATGGTATATG
GAAACTTACTCGCATTAAAACAGGAAAATTTAAAACGAATGCTCGCATATTCTTCGATTGCACACGCGGGTTACATTGTC
GCCGGGATTTCTGCTGGAATCAAAGAAGAGGTGTTATTCTATCTTATCGTATATTCTTTTATGAGTTTGGGAGCGTTTGC
TATTTTGGCGTATTTGGAGGATGGAACGAGACAAGTAACGTTCTTTTCCGTTCAGTCCTTGTCCGGGGTAAAACCTTTTA
CGGCGATCGCAATCAACATTTTCTTTATGTCCTTGGCGGGAGTTCCACCTTTTGGGGGATTCTGGGCGAAGTTGTTTCTC
TTTCAAAAGGTCGCCGAGTCGGAACATTTAATGAATCGAATCCTACTGATAGGAGGTGTGACGAATTCGGCCCTTGCCCT
TTATTATTATCTCCGGATTGGGATCGCCACTTTTATGAGTAGTGACGAGGGAGAAATCTCCCGCAATCACGCGGCTCCTC
ATAGTATCGGAGTGACTATAGTTGTAATGATTTGTCTTTTTGTGGTCGTGTTCGGTTGGTTCTTATTGGTTCCGGGTAAC
CTGCTTGCGATCGGTGTTTTACGGAATTTAAATTACTGA

Upstream 100 bases:

>100_bases
ACGAAGAGCGTCTCAAAGGATTTCAAAGTAAATATGCTTTACCTGGGTCGATCCGAAAAGGAAAAGAAACCACTCCCGAG
GGAGACGAGGAGGCTCTGAA

Downstream 100 bases:

>100_bases
TTCGTTTTAGAAAGCGAGGCTCCTCCTTCTGATTTACATTAGAAGGAACGCTCTCTTCCACGACTTCGCTTTGGCCGCAA
GCGGCCGCGTGTTTCGCGTC

Product: NADH dehydrogenase (ubiquinone), N chain

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MNFIPDGFDLFSILPALILATGGVFLICLSVLFKTKEFLIVRYVSGLVLLVAFGSVFYVAKFQYPGNGGTYFGGQIENSV
LSFWLNLIYLSMAIGTAAVAPRILKNHKIEFPEFYPLLLFATCGMILMTSGQDFILVFVALELMSICLYILIGMARSDLF
SLEATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITAALKPLVIAGYQGNFTKIGLVLFITGVAFKIALFPYHAWTPDAY
EGALTPVTGYMSTAAKAASIGLLLVLYTKLPLPLQDSTWAWLPGILALLSMVYGNLLALKQENLKRMLAYSSIAHAGYIV
AGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEDGTRQVTFFSVQSLSGVKPFTAIAINIFFMSLAGVPPFGGFWAKLFL
FQKVAESEHLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPHSIGVTIVVMICLFVVVFGWFLLVPGN
LLAIGVLRNLNY

Sequences:

>Translated_492_residues
MNFIPDGFDLFSILPALILATGGVFLICLSVLFKTKEFLIVRYVSGLVLLVAFGSVFYVAKFQYPGNGGTYFGGQIENSV
LSFWLNLIYLSMAIGTAAVAPRILKNHKIEFPEFYPLLLFATCGMILMTSGQDFILVFVALELMSICLYILIGMARSDLF
SLEATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITAALKPLVIAGYQGNFTKIGLVLFITGVAFKIALFPYHAWTPDAY
EGALTPVTGYMSTAAKAASIGLLLVLYTKLPLPLQDSTWAWLPGILALLSMVYGNLLALKQENLKRMLAYSSIAHAGYIV
AGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEDGTRQVTFFSVQSLSGVKPFTAIAINIFFMSLAGVPPFGGFWAKLFL
FQKVAESEHLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPHSIGVTIVVMICLFVVVFGWFLLVPGN
LLAIGVLRNLNY
>Mature_492_residues
MNFIPDGFDLFSILPALILATGGVFLICLSVLFKTKEFLIVRYVSGLVLLVAFGSVFYVAKFQYPGNGGTYFGGQIENSV
LSFWLNLIYLSMAIGTAAVAPRILKNHKIEFPEFYPLLLFATCGMILMTSGQDFILVFVALELMSICLYILIGMARSDLF
SLEATLKYFLLGSFSSGFMLMGIAFLFGGSGSTNITAALKPLVIAGYQGNFTKIGLVLFITGVAFKIALFPYHAWTPDAY
EGALTPVTGYMSTAAKAASIGLLLVLYTKLPLPLQDSTWAWLPGILALLSMVYGNLLALKQENLKRMLAYSSIAHAGYIV
AGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEDGTRQVTFFSVQSLSGVKPFTAIAINIFFMSLAGVPPFGGFWAKLFL
FQKVAESEHLMNRILLIGGVTNSALALYYYLRIGIATFMSSDEGEISRNHAAPHSIGVTIVVMICLFVVVFGWFLLVPGN
LLAIGVLRNLNY

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=400, Percent_Identity=33.5, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1788831, Length=321, Percent_Identity=25.2336448598131, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788827, Length=388, Percent_Identity=24.7422680412371, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI1788614, Length=353, Percent_Identity=21.8130311614731, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788829, Length=314, Percent_Identity=26.4331210191083, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53622; Mature: 53622

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFIPDGFDLFSILPALILATGGVFLICLSVLFKTKEFLIVRYVSGLVLLVAFGSVFYVA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
KFQYPGNGGTYFGGQIENSVLSFWLNLIYLSMAIGTAAVAPRILKNHKIEFPEFYPLLLF
EEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ATCGMILMTSGQDFILVFVALELMSICLYILIGMARSDLFSLEATLKYFLLGSFSSGFML
HHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
MGIAFLFGGSGSTNITAALKPLVIAGYQGNFTKIGLVLFITGVAFKIALFPYHAWTPDAY
HHHHHHHCCCCCCCHHEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCC
EGALTPVTGYMSTAAKAASIGLLLVLYTKLPLPLQDSTWAWLPGILALLSMVYGNLLALK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QENLKRMLAYSSIAHAGYIVAGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEDGTRQVT
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FFSVQSLSGVKPFTAIAINIFFMSLAGVPPFGGFWAKLFLFQKVAESEHLMNRILLIGGV
EEEEHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
TNSALALYYYLRIGIATFMSSDEGEISRNHAAPHSIGVTIVVMICLFVVVFGWFLLVPGN
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
LLAIGVLRNLNY
HHHHHHHHCCCC
>Mature Secondary Structure
MNFIPDGFDLFSILPALILATGGVFLICLSVLFKTKEFLIVRYVSGLVLLVAFGSVFYVA
CCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEE
KFQYPGNGGTYFGGQIENSVLSFWLNLIYLSMAIGTAAVAPRILKNHKIEFPEFYPLLLF
EEEECCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ATCGMILMTSGQDFILVFVALELMSICLYILIGMARSDLFSLEATLKYFLLGSFSSGFML
HHCCHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
MGIAFLFGGSGSTNITAALKPLVIAGYQGNFTKIGLVLFITGVAFKIALFPYHAWTPDAY
HHHHHHHCCCCCCCHHEEECCEEEECCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCC
EGALTPVTGYMSTAAKAASIGLLLVLYTKLPLPLQDSTWAWLPGILALLSMVYGNLLALK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QENLKRMLAYSSIAHAGYIVAGISAGIKEEVLFYLIVYSFMSLGAFAILAYLEDGTRQVT
HHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
FFSVQSLSGVKPFTAIAINIFFMSLAGVPPFGGFWAKLFLFQKVAESEHLMNRILLIGGV
EEEEHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEECC
TNSALALYYYLRIGIATFMSSDEGEISRNHAAPHSIGVTIVVMICLFVVVFGWFLLVPGN
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
LLAIGVLRNLNY
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]