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The map label for this gene is rlmN [H]

Identifier: 116328894

GI number: 116328894

Start: 2705513

End: 2706568

Strand: Reverse

Name: rlmN [H]

Synonym: LBL_2285

Alternate gene names: 116328894

Gene position: 2706568-2705513 (Counterclockwise)

Preceding gene: 116328896

Following gene: 116328893

Centisome position: 74.88

GC content: 43.18

Gene sequence:

>1056_bases
ATGACGCAAGAGATTCTCGGAGAAGTTCAAACCGGAAAAATTCCGTTAAAAGGAAGAACCTTAAAGGAACTTTCCGAGAT
CATGGTTTCTCTCGGAGAAAAATCTTTTCGCGCAAAACAGATCTATCACGGATTATACGTAAACCGTTATGAGTCTTGGG
AACAATTTACCACATTTTCCAAAACGTTAAAGGAGAAGTTAGAGGGCCTTTGTTCTCTGACACAACTTACAGTTGTCAAA
CATCTTAAATCCGTGGACGGAACTCAAAAGTTCACGTTCGCATCCGAACAAGGAAAGGAATTCGAAGCCGTTTGGATCCC
ATCCGGAGACGGAGGAAGAAAGACGATTTGCATTTCTTCCCAGGTCGGCTGTACTCTCAATTGTAAATTTTGTGCGACCG
CAAAATTGGAATTTCAAGGAAACCTAAAGGCCCATGAGATCGTGGACCAGGTTCTCCAGGTGGAAAAAATCGTAGGAGAC
AATGCGACTAACGTTGTTTTTATGGGAATGGGGGAGCCGTTCCACAATTACTTCAACGTGATACGCGCGGCTTCTATTCT
TCATGATCCCGACGCGCTTAATCTGGGGGCCAAAAGAATTACGATTTCCACATCCGGAGTGGTCAACGGTATCAGACGTT
TTATCGAAAATAAGGAACCTTATAATTTTGCGATCTCTCTCAATCATCCTGATCCGAACGGAAGATTACAAATCATGGAC
ATCGAAGAGAAGTTTGCTCTTTCCGAACTTTTACAAGCTGCAAAAGATTTTACAAGAGAGTTAAAAAGAAGAATCACTTT
CGAATATGTGATGATTCCCGGTGTCAGCATGGGACCCGAGAACGCAAACAAGCTCGTAAAAATCGCAAGATCTCTTGATT
GTAAGATCAATGTGATTCCGCTTAATACCGAATTTTTCGGATGGAGAAGACCCACAAAGCAAGAGGTCGCGGAATTCATA
ACTCTTTTGGAACCGGCCGGAGTTCCGATTCTCAACAGAAGATCGCCCGGAAAAGATATCTTCGGCGCTTGTGGAATGCT
CGCTTCAAAAAGTTGA

Upstream 100 bases:

>100_bases
GGATACAAAGACGGAAAAGCTGAATTGGAACATGAGAATCCGTAAAATGAGACTGTCCTCTATGGGGGGCTTGAAAAAAA
TCGACAATAAGAACATCCTT

Downstream 100 bases:

>100_bases
CCTCGGTTTTCGTTTCTGAAAAATGGGGCAATGAATTCAAAAAGAACTTTTATTCTTTTTTTATGTCTTTTTTCTTTCGT
TTCCTGCCAGGAATATGTTC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 351; Mature: 350

Protein sequence:

>351_residues
MTQEILGEVQTGKIPLKGRTLKELSEIMVSLGEKSFRAKQIYHGLYVNRYESWEQFTTFSKTLKEKLEGLCSLTQLTVVK
HLKSVDGTQKFTFASEQGKEFEAVWIPSGDGGRKTICISSQVGCTLNCKFCATAKLEFQGNLKAHEIVDQVLQVEKIVGD
NATNVVFMGMGEPFHNYFNVIRAASILHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPNGRLQIMD
IEEKFALSELLQAAKDFTRELKRRITFEYVMIPGVSMGPENANKLVKIARSLDCKINVIPLNTEFFGWRRPTKQEVAEFI
TLLEPAGVPILNRRSPGKDIFGACGMLASKS

Sequences:

>Translated_351_residues
MTQEILGEVQTGKIPLKGRTLKELSEIMVSLGEKSFRAKQIYHGLYVNRYESWEQFTTFSKTLKEKLEGLCSLTQLTVVK
HLKSVDGTQKFTFASEQGKEFEAVWIPSGDGGRKTICISSQVGCTLNCKFCATAKLEFQGNLKAHEIVDQVLQVEKIVGD
NATNVVFMGMGEPFHNYFNVIRAASILHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPNGRLQIMD
IEEKFALSELLQAAKDFTRELKRRITFEYVMIPGVSMGPENANKLVKIARSLDCKINVIPLNTEFFGWRRPTKQEVAEFI
TLLEPAGVPILNRRSPGKDIFGACGMLASKS
>Mature_350_residues
TQEILGEVQTGKIPLKGRTLKELSEIMVSLGEKSFRAKQIYHGLYVNRYESWEQFTTFSKTLKEKLEGLCSLTQLTVVKH
LKSVDGTQKFTFASEQGKEFEAVWIPSGDGGRKTICISSQVGCTLNCKFCATAKLEFQGNLKAHEIVDQVLQVEKIVGDN
ATNVVFMGMGEPFHNYFNVIRAASILHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPNGRLQIMDI
EEKFALSELLQAAKDFTRELKRRITFEYVMIPGVSMGPENANKLVKIARSLDCKINVIPLNTEFFGWRRPTKQEVAEFIT
LLEPAGVPILNRRSPGKDIFGACGMLASKS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=351, Percent_Identity=36.4672364672365, Blast_Score=204, Evalue=6e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 39255; Mature: 39124

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQEILGEVQTGKIPLKGRTLKELSEIMVSLGEKSFRAKQIYHGLYVNRYESWEQFTTFS
CCHHHHCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLKEKLEGLCSLTQLTVVKHLKSVDGTQKFTFASEQGKEFEAVWIPSGDGGRKTICISS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEEC
QVGCTLNCKFCATAKLEFQGNLKAHEIVDQVLQVEKIVGDNATNVVFMGMGEPFHNYFNV
CCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
IRAASILHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPNGRLQIMD
HHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEE
IEEKFALSELLQAAKDFTRELKRRITFEYVMIPGVSMGPENANKLVKIARSLDCKINVIP
CHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEE
LNTEFFGWRRPTKQEVAEFITLLEPAGVPILNRRSPGKDIFGACGMLASKS
ECCHHCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
TQEILGEVQTGKIPLKGRTLKELSEIMVSLGEKSFRAKQIYHGLYVNRYESWEQFTTFS
CHHHHCCCCCCCEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLKEKLEGLCSLTQLTVVKHLKSVDGTQKFTFASEQGKEFEAVWIPSGDGGRKTICISS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEEECCCCCCEEEEEEC
QVGCTLNCKFCATAKLEFQGNLKAHEIVDQVLQVEKIVGDNATNVVFMGMGEPFHNYFNV
CCCEEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
IRAASILHDPDALNLGAKRITISTSGVVNGIRRFIENKEPYNFAISLNHPDPNGRLQIMD
HHHHHHHCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEE
IEEKFALSELLQAAKDFTRELKRRITFEYVMIPGVSMGPENANKLVKIARSLDCKINVIP
CHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHCCCEEEEEE
LNTEFFGWRRPTKQEVAEFITLLEPAGVPILNRRSPGKDIFGACGMLASKS
ECCHHCCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA