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The map label for this gene is lplA [C]

Identifier: 116328754

GI number: 116328754

Start: 2491964

End: 2492941

Strand: Reverse

Name: lplA [C]

Synonym: LBL_2128

Alternate gene names: 116328754

Gene position: 2492941-2491964 (Counterclockwise)

Preceding gene: 116328755

Following gene: 116328753

Centisome position: 68.97

GC content: 41.31

Gene sequence:

>978_bases
ATGCGTACATTTGCATTCTTACAAGACTCGAACAGATCCGCCGCTTACAATCTTGCCATTGAAGAAGCGATCGGACTTCA
TTTCGTATCGTCCGGTTACGGAGCGGGACTTAGAATCTGGAGGAATCCGTTTTCCATCGTACTTGGACTTTCGGAGCAGG
CGGAAGATACGATTTTACCGGAAGTTTTGAGGAATTTTATGGAATCGGGTGGAAAAGAGAGAAGTGCGATTTTGCCGGAA
ATTCCGAACTTAACTTTGGAATCCGAAAAGAACCGAGCGAATTCGACCACGTTAAGAAATCAAAATTCGATTTCAAAAAA
CCGGAAAAAAATTTCCGGAATAAACGTTTCGAATTCTATTCGGGGTTTCAAAGATAGAAAACGTTTCGACAAAGAATTTC
CCACAATCGTTCGAAGAGCGAGCGGAGGAGGGACGGTAGTTCATCATCCCGAGGAGAATCTGAATTTCACTTTTTTCGTT
TCCTTGGATGTAAAACCTGAACTCTACAAAGTAAAGGAATCCTACGGTTATTTTTTAAGTCTTGTAGTAAACGCGTTAAA
GCGACAAACGTTAAGCGCTTCTTGTCGTGGAAAATCGGATCTTGCTATTTTGGAACAAGGTCTGGAAAAAAAAATTTCCG
GAAACGCTCAGTTTCGAAAAAGAAATGCGGTTATACATCACGGTACCTTGATCTTAAAACCTTCTCTCATCGAAAGGGTT
TCCGGACTGCTCAAACATCCTCCTGAGGAACCCGAATATAGAAAGAATCGAAAACATTCCGATTTTGTGACGTCTCTTCC
GAGCGATTTTTCTCCCCTAAAATTTAGCCAAGATCTTTCTCATGTATTTGCCGAATCCTTGGGTCTTTTCAGAATGGGCT
CAGAGAGGGATCTCCGATTTACAAAAGCGGTTTTCCAGGAAGCGAAACAACTTTTGGAAAGTAAATATTCTAGGATGGAT
TTTATCCTGAGAAACTAA

Upstream 100 bases:

>100_bases
GATCGTTTCGAAGGGATCCTTTATTCTCAAAAGTGAATATTTGAAATTGTAGGTATTCAAAAAATTTGAAATAAAATCCG
GACTTTTGTCCTCATATCTT

Downstream 100 bases:

>100_bases
ATCGGATTTAGAACCCGTCTCAAAACCTTAGGAATGTAAGAACTATTACAATTTTAAGCGACAGGAGAAAACCGCCAATG
CGACGGTTTCTGTGGGAACT

Product: lipoate-protein ligase A

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: Lipoyltransferase And Lipoate-Protein Ligase; Lipoate Protein Ligase A; Lipoate-Protein Ligase A ; Lipoate-Protein Ligase; Biotin/Lipoate A/B Protein Ligase

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRTFAFLQDSNRSAAYNLAIEEAIGLHFVSSGYGAGLRIWRNPFSIVLGLSEQAEDTILPEVLRNFMESGGKERSAILPE
IPNLTLESEKNRANSTTLRNQNSISKNRKKISGINVSNSIRGFKDRKRFDKEFPTIVRRASGGGTVVHHPEENLNFTFFV
SLDVKPELYKVKESYGYFLSLVVNALKRQTLSASCRGKSDLAILEQGLEKKISGNAQFRKRNAVIHHGTLILKPSLIERV
SGLLKHPPEEPEYRKNRKHSDFVTSLPSDFSPLKFSQDLSHVFAESLGLFRMGSERDLRFTKAVFQEAKQLLESKYSRMD
FILRN

Sequences:

>Translated_325_residues
MRTFAFLQDSNRSAAYNLAIEEAIGLHFVSSGYGAGLRIWRNPFSIVLGLSEQAEDTILPEVLRNFMESGGKERSAILPE
IPNLTLESEKNRANSTTLRNQNSISKNRKKISGINVSNSIRGFKDRKRFDKEFPTIVRRASGGGTVVHHPEENLNFTFFV
SLDVKPELYKVKESYGYFLSLVVNALKRQTLSASCRGKSDLAILEQGLEKKISGNAQFRKRNAVIHHGTLILKPSLIERV
SGLLKHPPEEPEYRKNRKHSDFVTSLPSDFSPLKFSQDLSHVFAESLGLFRMGSERDLRFTKAVFQEAKQLLESKYSRMD
FILRN
>Mature_325_residues
MRTFAFLQDSNRSAAYNLAIEEAIGLHFVSSGYGAGLRIWRNPFSIVLGLSEQAEDTILPEVLRNFMESGGKERSAILPE
IPNLTLESEKNRANSTTLRNQNSISKNRKKISGINVSNSIRGFKDRKRFDKEFPTIVRRASGGGTVVHHPEENLNFTFFV
SLDVKPELYKVKESYGYFLSLVVNALKRQTLSASCRGKSDLAILEQGLEKKISGNAQFRKRNAVIHHGTLILKPSLIERV
SGLLKHPPEEPEYRKNRKHSDFVTSLPSDFSPLKFSQDLSHVFAESLGLFRMGSERDLRFTKAVFQEAKQLLESKYSRMD
FILRN

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.2.- [C]

Molecular weight: Translated: 36855; Mature: 36855

Theoretical pI: Translated: 10.42; Mature: 10.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTFAFLQDSNRSAAYNLAIEEAIGLHFVSSGYGAGLRIWRNPFSIVLGLSEQAEDTILP
CCCEEEEECCCCCEEEEEHHHHHHHHEEECCCCCCCCEEECCCCCEEEECCHHHHHHHHH
EVLRNFMESGGKERSAILPEIPNLTLESEKNRANSTTLRNQNSISKNRKKISGINVSNSI
HHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCHHHH
RGFKDRKRFDKEFPTIVRRASGGGTVVHHPEENLNFTFFVSLDVKPELYKVKESYGYFLS
CCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LVVNALKRQTLSASCRGKSDLAILEQGLEKKISGNAQFRKRNAVIHHGTLILKPSLIERV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCCEEECCEEEECHHHHHHH
SGLLKHPPEEPEYRKNRKHSDFVTSLPSDFSPLKFSQDLSHVFAESLGLFRMGSERDLRF
HHHHHCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
TKAVFQEAKQLLESKYSRMDFILRN
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRTFAFLQDSNRSAAYNLAIEEAIGLHFVSSGYGAGLRIWRNPFSIVLGLSEQAEDTILP
CCCEEEEECCCCCEEEEEHHHHHHHHEEECCCCCCCCEEECCCCCEEEECCHHHHHHHHH
EVLRNFMESGGKERSAILPEIPNLTLESEKNRANSTTLRNQNSISKNRKKISGINVSNSI
HHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCCHHCCCCHHHHHHHHHHCCCCCHHHH
RGFKDRKRFDKEFPTIVRRASGGGTVVHHPEENLNFTFFVSLDVKPELYKVKESYGYFLS
CCHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHH
LVVNALKRQTLSASCRGKSDLAILEQGLEKKISGNAQFRKRNAVIHHGTLILKPSLIERV
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHCCCEEECCEEEECHHHHHHH
SGLLKHPPEEPEYRKNRKHSDFVTSLPSDFSPLKFSQDLSHVFAESLGLFRMGSERDLRF
HHHHHCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
TKAVFQEAKQLLESKYSRMDFILRN
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA