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The map label for this gene is glyA

Identifier: 116328753

GI number: 116328753

Start: 2490519

End: 2491766

Strand: Reverse

Name: glyA

Synonym: LBL_2127

Alternate gene names: 116328753

Gene position: 2491766-2490519 (Counterclockwise)

Preceding gene: 116328754

Following gene: 116328750

Centisome position: 68.94

GC content: 46.31

Gene sequence:

>1248_bases
ATGCAGTTTCTTCCGAAAGCGGATCCAGAGATATTCGCAGCATTAAAAAAAGAGGACGAAAGACAGGAAAATAACCTGGA
AATGATCGCCTCCGAAAATTTTGTTTCCAGAGCCGTTCTGGAGGCCTATACTTCCACGCTCACAAACAAATACGCCGAAG
GTTATCCGGGAAAAAGATACTACAACGGTTGTCACAACGCCGATATCGTAGAGAGTCTCGCTATTGAAAGAGCAAAAGAA
CTTTTCGGTGCGGAATACGCTAACGTTCAACCGCATTCCGGCGCCCAGGCAAACATGGCGGTTTTTCTTGCTTGTTTGGA
GCCCGGGGATTCTTTTTTAGGAATGAATCTTGCGCACGGTGGGCATTTGACTCACGGTTCTCCCGTCAACGTAAGCGGCA
GAATTTATAAACCGATTCCTTACGGAGTCGATTCAAAAACAGAAACAATCGACTATGATGAGATCGCGAAACTCGCAAGG
GAGCACAAACCGAAGTTGATTGTTGCGGGCGCTTCCGCTTACGCAAGGACCATCGACTTTTCTAAGTTTGCCGAAATTGC
GAAGGAGGTAGGCGCTAAACTGATGGCGGACATCGCTCATATTTCCGGTCTTGTTTCCACAGGTTATCATCCTTCTCCGG
TCGGGCTTTTCGATTTTGTGACTACTACCACTCATAAAACGTTGAGAGGACCGAGGGGTGGACTCATTTTATCTACATTA
GAAAATGAGAAAGTTTTAAACTCTCGGGTTTTCCCCGGAATTCAAGGTGGACCTTTGATGCATGTGATCGCGGCCAAAGC
GGTCGCGTTTAAAGAGGCTCTTCAACCGGAATATAAGAAGTATATCGAAATCGTTCTTGCGAATGCGAAAACTCTCGCGG
AAGTTTTTTTAAAACGAGGCTACAGAGTCGTAAGCGGTGGAACGGATAACCATTTGGTTCTTTTGGATGTTTCTGTAAAA
GGACTTACCGGTGTACAAGCGGCCGACGGACTGGACGAAGTCGGAGTGACCGTGAACAAAAACGCAATTCCTTTCGATAA
GAATCCTCCGGCGGTCGCTTCCGGAATTCGTCTTGGGACTCCGGCTCTCACCACTCGAGGTCTAAAACCCGCGGATATGG
AAACGGTTGGAAATCTAATCTGCGATTTTTTAGACAATCCGAACGAAGAGAAAAATAAAAAGAGGGTCAAGGGCGGAGTT
CAAGAGATTACTCGAAAGTTTCCGATGGATCAATTCCGTTTAGATTAA

Upstream 100 bases:

>100_bases
ACTCTCACGTTTTTTAAAAACTTGCCGGATCGTTTAAAGATATTTTTCTTTAGGTTTTGAGACGGGTTCTTACTCGGAAT
TATCAAAAAAGAAGGGCCAG

Downstream 100 bases:

>100_bases
AAATTTTTAAATCGACATTGAAGATATTCCATCCGGAACCATAAAATAGAGTTGTTGAAAAATTCCATGATTCGGTTTAA
CAAAACTGCTTCAATCGATC

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRYYNGCHNADIVESLAIERAKE
LFGAEYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHGGHLTHGSPVNVSGRIYKPIPYGVDSKTETIDYDEIAKLAR
EHKPKLIVAGASAYARTIDFSKFAEIAKEVGAKLMADIAHISGLVSTGYHPSPVGLFDFVTTTTHKTLRGPRGGLILSTL
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFKEALQPEYKKYIEIVLANAKTLAEVFLKRGYRVVSGGTDNHLVLLDVSVK
GLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETVGNLICDFLDNPNEEKNKKRVKGGV
QEITRKFPMDQFRLD

Sequences:

>Translated_415_residues
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRYYNGCHNADIVESLAIERAKE
LFGAEYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHGGHLTHGSPVNVSGRIYKPIPYGVDSKTETIDYDEIAKLAR
EHKPKLIVAGASAYARTIDFSKFAEIAKEVGAKLMADIAHISGLVSTGYHPSPVGLFDFVTTTTHKTLRGPRGGLILSTL
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFKEALQPEYKKYIEIVLANAKTLAEVFLKRGYRVVSGGTDNHLVLLDVSVK
GLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETVGNLICDFLDNPNEEKNKKRVKGGV
QEITRKFPMDQFRLD
>Mature_415_residues
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRYYNGCHNADIVESLAIERAKE
LFGAEYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHGGHLTHGSPVNVSGRIYKPIPYGVDSKTETIDYDEIAKLAR
EHKPKLIVAGASAYARTIDFSKFAEIAKEVGAKLMADIAHISGLVSTGYHPSPVGLFDFVTTTTHKTLRGPRGGLILSTL
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFKEALQPEYKKYIEIVLANAKTLAEVFLKRGYRVVSGGTDNHLVLLDVSVK
GLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGTPALTTRGLKPADMETVGNLICDFLDNPNEEKNKKRVKGGV
QEITRKFPMDQFRLD

Specific function: Interconversion of serine and glycine

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family

Homologues:

Organism=Homo sapiens, GI19923315, Length=456, Percent_Identity=43.640350877193, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI261862352, Length=456, Percent_Identity=43.640350877193, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI261862350, Length=456, Percent_Identity=43.640350877193, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI261862348, Length=456, Percent_Identity=43.640350877193, Blast_Score=352, Evalue=3e-97,
Organism=Homo sapiens, GI261862346, Length=456, Percent_Identity=42.5438596491228, Blast_Score=334, Evalue=7e-92,
Organism=Homo sapiens, GI22547186, Length=436, Percent_Identity=42.6605504587156, Blast_Score=319, Evalue=4e-87,
Organism=Homo sapiens, GI22547189, Length=421, Percent_Identity=40.6175771971496, Blast_Score=286, Evalue=2e-77,
Organism=Escherichia coli, GI1788902, Length=405, Percent_Identity=58.7654320987654, Blast_Score=468, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25144732, Length=398, Percent_Identity=46.9849246231156, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI25144729, Length=398, Percent_Identity=46.9849246231156, Blast_Score=343, Evalue=1e-94,
Organism=Saccharomyces cerevisiae, GI6319739, Length=399, Percent_Identity=45.1127819548872, Blast_Score=342, Evalue=7e-95,
Organism=Saccharomyces cerevisiae, GI6323087, Length=437, Percent_Identity=40.9610983981693, Blast_Score=314, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24640005, Length=403, Percent_Identity=49.1315136476427, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI221329721, Length=403, Percent_Identity=49.1315136476427, Blast_Score=370, Evalue=1e-103,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): GLYA_LEPBJ (Q04R46)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_801382.1
- ProteinModelPortal:   Q04R46
- SMR:   Q04R46
- STRING:   Q04R46
- GeneID:   4410256
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_2130
- eggNOG:   COG0112
- HOGENOM:   HBG301263
- OMA:   GGLIMTK
- PhylomeDB:   Q04R46
- ProtClustDB:   PRK00011
- BioCyc:   LBOR355277:LBJ_2130-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00051_B
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11680
- PIRSF:   PIRSF000412

Pfam domain/function: PF00464 SHMT; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.1.2.1

Molecular weight: Translated: 45085; Mature: 45085

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRY
CCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNGCHNADIVESLAIERAKELFGAEYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHG
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECEEECCC
GHLTHGSPVNVSGRIYKPIPYGVDSKTETIDYDEIAKLAREHKPKLIVAGASAYARTIDF
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCH
SKFAEIAKEVGAKLMADIAHISGLVSTGYHPSPVGLFDFVTTTTHKTLRGPRGGLILSTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFKEALQPEYKKYIEIVLANAKTLAEVFLKRG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHCC
YRVVSGGTDNHLVLLDVSVKGLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGT
CEEEECCCCCEEEEEEEEECCCCCCHHHCCHHHCCEEECCCCCCCCCCCCHHHCCCCCCC
PALTTRGLKPADMETVGNLICDFLDNPNEEKNKKRVKGGVQEITRKFPMDQFRLD
CHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure
MQFLPKADPEIFAALKKEDERQENNLEMIASENFVSRAVLEAYTSTLTNKYAEGYPGKRY
CCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
YNGCHNADIVESLAIERAKELFGAEYANVQPHSGAQANMAVFLACLEPGDSFLGMNLAHG
HCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCEECEEECCC
GHLTHGSPVNVSGRIYKPIPYGVDSKTETIDYDEIAKLAREHKPKLIVAGASAYARTIDF
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEECCHHHHHHCCH
SKFAEIAKEVGAKLMADIAHISGLVSTGYHPSPVGLFDFVTTTTHKTLRGPRGGLILSTL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEC
ENEKVLNSRVFPGIQGGPLMHVIAAKAVAFKEALQPEYKKYIEIVLANAKTLAEVFLKRG
CCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHHHCC
YRVVSGGTDNHLVLLDVSVKGLTGVQAADGLDEVGVTVNKNAIPFDKNPPAVASGIRLGT
CEEEECCCCCEEEEEEEEECCCCCCHHHCCHHHCCEEECCCCCCCCCCCCHHHCCCCCCC
PALTTRGLKPADMETVGNLICDFLDNPNEEKNKKRVKGGVQEITRKFPMDQFRLD
CHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA