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The map label for this gene is smpB

Identifier: 116328638

GI number: 116328638

Start: 2333790

End: 2334272

Strand: Reverse

Name: smpB

Synonym: LBL_2001

Alternate gene names: 116328638

Gene position: 2334272-2333790 (Counterclockwise)

Preceding gene: 116328639

Following gene: 116328637

Centisome position: 64.58

GC content: 41.82

Gene sequence:

>483_bases
ATGGCAAATAAAAAAGAAGAACCCGGACATTCTCCTCTGGTCAATAAAAAGGCCAAGTTCAACTTCGAGTTAGTTTCGTT
TATCGAGGCGGGAATCGTCTTGTCCGGATCGGAAGTCAAAAGCCTCCGTGAAAAAAAAGGAAATCTCACCGACGCATTTG
CCAAGATCAAAAATGGGGAAGTGTTTTTGGAGAATTTTTCAATCACTCCATATAAAAACGGAGGTTATGTCAATCATCCC
GAAATTCGTCCGAGAAAACTCCTCCTTCATAAGAAGGAAATCGAAAAATTAGAACGTCAGGTAAAAGAAAAAGGTTTGGT
TCTCGTCGCTACTAAAGTTTATTTTAAGAATAATCTTCGCGTAAAGGTGGAGATCGCCGTGGGCAAACCGAAAAAGATCC
ACGATAAACGGGATGACATGCAGAAAAAAGACGCGCAACAGGAAATCGCCCGCGCGTTAAAATCATCCAACCGCTACGAG
TAA

Upstream 100 bases:

>100_bases
TTCTTACAAAAACGGTAGGATATCAGATAGGCTTTAGGAAAAAAAATTCCTAAAATTGATACAATCAAACCGGCCACGAA
GTCGGTTTAAAAAAAGTAAG

Downstream 100 bases:

>100_bases
TACGCGATTTAAAACCAGGAATACAATGGCTAAAGCCGTAAGAAAATCGAATTCGGAAGAAACCGTTCCGATCAAGGCTC
CCCGCAAAGCGCCCGGAGAA

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MANKKEEPGHSPLVNKKAKFNFELVSFIEAGIVLSGSEVKSLREKKGNLTDAFAKIKNGEVFLENFSITPYKNGGYVNHP
EIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLRVKVEIAVGKPKKIHDKRDDMQKKDAQQEIARALKSSNRYE

Sequences:

>Translated_160_residues
MANKKEEPGHSPLVNKKAKFNFELVSFIEAGIVLSGSEVKSLREKKGNLTDAFAKIKNGEVFLENFSITPYKNGGYVNHP
EIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLRVKVEIAVGKPKKIHDKRDDMQKKDAQQEIARALKSSNRYE
>Mature_159_residues
ANKKEEPGHSPLVNKKAKFNFELVSFIEAGIVLSGSEVKSLREKKGNLTDAFAKIKNGEVFLENFSITPYKNGGYVNHPE
IRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLRVKVEIAVGKPKKIHDKRDDMQKKDAQQEIARALKSSNRYE

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=157, Percent_Identity=38.2165605095541, Blast_Score=105, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_LEPBJ (Q04TV5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800423.1
- ProteinModelPortal:   Q04TV5
- SMR:   Q04TV5
- STRING:   Q04TV5
- GeneID:   4412058
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1033
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- ProtClustDB:   PRK05422
- BioCyc:   LBOR355277:LBJ_1033-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18330; Mature: 18198

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKKEEPGHSPLVNKKAKFNFELVSFIEAGIVLSGSEVKSLREKKGNLTDAFAKIKNGE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEECCHHHHHHHHHCCCHHHHHHHHCCCE
VFLENFSITPYKNGGYVNHPEIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLR
EEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCE
VKVEIAVGKPKKIHDKRDDMQKKDAQQEIARALKSSNRYE
EEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ANKKEEPGHSPLVNKKAKFNFELVSFIEAGIVLSGSEVKSLREKKGNLTDAFAKIKNGE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCEEECCHHHHHHHHHCCCHHHHHHHHCCCE
VFLENFSITPYKNGGYVNHPEIRPRKLLLHKKEIEKLERQVKEKGLVLVATKVYFKNNLR
EEEEECCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCE
VKVEIAVGKPKKIHDKRDDMQKKDAQQEIARALKSSNRYE
EEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA