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The map label for this gene is glnA [H]

Identifier: 116328630

GI number: 116328630

Start: 2321637

End: 2323058

Strand: Reverse

Name: glnA [H]

Synonym: LBL_1991

Alternate gene names: 116328630

Gene position: 2323058-2321637 (Counterclockwise)

Preceding gene: 116328632

Following gene: 116328624

Centisome position: 64.27

GC content: 44.37

Gene sequence:

>1422_bases
ATGTCCAGAACACCTAGTGAAGTTATCGCATATGCCAAGGCGAATAACGTTCTTTTCTACGATTTCCGTTTTACGGATAT
CAAAGGAGCTTGGCACCACGTATCGTATCACACGATCGCGATTACCGAGGATTCCTTTAAAGGGTTACCGTTTGATGGAA
GTTCCATACCTGCTTGGCAACCGATCGATCGATCCGATATGCAGTTGATTCCGGATACCGATGCGATTTTTTTAGATCCT
TTTACTGCGGATCCTACTCTTGTCGTTTTCTGCGACGTTTTTGACATCTACAAAGGCACTCTTTACGAAAAATGTCCTCG
TTCTATCGCGAAAAAAGCCCTGAAGTATCTTGAGTCCTCCGGTCTTGCAGACACTGCGTATTTCGGTCCTGAAAACGAAT
TTTTTATCTTTGACAACATTAAAGTAAGAGACGCGATCAACGTTCAATACTACGAAATTGATTCTTCGGAAGGGATTTGG
AATTCCCACACCGACTTTCCGGGTTCCACAAACACAGGTCACCGTCCCGGCACCAAAGGCGGTTACTTTCCGGTGGCTCC
TGTGGATTCTCAAGTGGATCTGAGAGTTGCGATCGTTAAGACTCTTCATCAAATTGGTATGGAAACTTTTGTGGTCCATC
ACGAGGTTGCTCAAGGTCAAGGTGAAATCGGCGTTAAATTCGGAACTTTGATTGAAGCGGCGGATAACGTTCAAAAACTG
AAATACGTTGTTAAGATGGTGGCTCATAATTACGGTAAAACCGCAACCTTCATGCCGAAACCTCTTTATGGTGACAACGG
TAACGGTATGCATTGTCACCAGTCCATTTGGAAAAACGGTGTGAACTTGTTCGCCGGTAAAGGTTACCAAAACTTAAGCG
ACACCGCTATGAATTACATCGGCGGGGTTCTTTCTCATGCAAAAGCCTGTGCGGCGTTTACGAACGCTTCCACGAATTCT
TATAAAAGACTTCTCCCCGGTTTCGAAGCTCCTTCCATTTTAGCTTATTCCGCTCAGAACCGTTCCGCTTCTTGCCGTAT
TCCTTTCGTAAATGGGGACAAAGCAAGAAGGGTTGAGTTCCGTTTCCCTGATTCTTCCTCAAACCCTTATCTTGCTTTTG
CAGCGATGCTTATGGCCGGTATCGACGGAGTTCAAAAGAAAATTGATCCGGGTCCGCCTCGGGAAGAGGATCTTTTCGAA
CTTTCTCTGGATGAAATTCGTGAAAAAGGAATTCAACAAATGCCTCACACTCTTCGTGAATCCGTAGAACACATGCTCTT
GGATAAAGAGTTCCTCAAAAAAGGGGACGTATTTACGGAAGAATTCATTCAAACTTACAAAGCGTATAAGTTTGAAACTG
AAATTTGGCCTTGGGAAGGTAGACCTCACCCGTTTGAATTTCTTACTACGTATTCCTGCTGA

Upstream 100 bases:

>100_bases
CATCGGATTTCAGTTCAATGGTACGATATTTGCACTAAAAAGTACAGACGCATCATGCGTGAATTAACGATTAATACTCG
AAGACTAACAGGAGAATAGA

Downstream 100 bases:

>100_bases
TCATCGGATCGATCGAGCGCCGGCGTCAAAAGTGACGATGTTCGTTTAGATTACGGATCGAAAAGCCCGGGATTTTTCTC
GGGCTTTTTTTGGATGGATT

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSRTPSEVIAYAKANNVLFYDFRFTDIKGAWHHVSYHTIAITEDSFKGLPFDGSSIPAWQPIDRSDMQLIPDTDAIFLDP
FTADPTLVVFCDVFDIYKGTLYEKCPRSIAKKALKYLESSGLADTAYFGPENEFFIFDNIKVRDAINVQYYEIDSSEGIW
NSHTDFPGSTNTGHRPGTKGGYFPVAPVDSQVDLRVAIVKTLHQIGMETFVVHHEVAQGQGEIGVKFGTLIEAADNVQKL
KYVVKMVAHNYGKTATFMPKPLYGDNGNGMHCHQSIWKNGVNLFAGKGYQNLSDTAMNYIGGVLSHAKACAAFTNASTNS
YKRLLPGFEAPSILAYSAQNRSASCRIPFVNGDKARRVEFRFPDSSSNPYLAFAAMLMAGIDGVQKKIDPGPPREEDLFE
LSLDEIREKGIQQMPHTLRESVEHMLLDKEFLKKGDVFTEEFIQTYKAYKFETEIWPWEGRPHPFEFLTTYSC

Sequences:

>Translated_473_residues
MSRTPSEVIAYAKANNVLFYDFRFTDIKGAWHHVSYHTIAITEDSFKGLPFDGSSIPAWQPIDRSDMQLIPDTDAIFLDP
FTADPTLVVFCDVFDIYKGTLYEKCPRSIAKKALKYLESSGLADTAYFGPENEFFIFDNIKVRDAINVQYYEIDSSEGIW
NSHTDFPGSTNTGHRPGTKGGYFPVAPVDSQVDLRVAIVKTLHQIGMETFVVHHEVAQGQGEIGVKFGTLIEAADNVQKL
KYVVKMVAHNYGKTATFMPKPLYGDNGNGMHCHQSIWKNGVNLFAGKGYQNLSDTAMNYIGGVLSHAKACAAFTNASTNS
YKRLLPGFEAPSILAYSAQNRSASCRIPFVNGDKARRVEFRFPDSSSNPYLAFAAMLMAGIDGVQKKIDPGPPREEDLFE
LSLDEIREKGIQQMPHTLRESVEHMLLDKEFLKKGDVFTEEFIQTYKAYKFETEIWPWEGRPHPFEFLTTYSC
>Mature_472_residues
SRTPSEVIAYAKANNVLFYDFRFTDIKGAWHHVSYHTIAITEDSFKGLPFDGSSIPAWQPIDRSDMQLIPDTDAIFLDPF
TADPTLVVFCDVFDIYKGTLYEKCPRSIAKKALKYLESSGLADTAYFGPENEFFIFDNIKVRDAINVQYYEIDSSEGIWN
SHTDFPGSTNTGHRPGTKGGYFPVAPVDSQVDLRVAIVKTLHQIGMETFVVHHEVAQGQGEIGVKFGTLIEAADNVQKLK
YVVKMVAHNYGKTATFMPKPLYGDNGNGMHCHQSIWKNGVNLFAGKGYQNLSDTAMNYIGGVLSHAKACAAFTNASTNSY
KRLLPGFEAPSILAYSAQNRSASCRIPFVNGDKARRVEFRFPDSSSNPYLAFAAMLMAGIDGVQKKIDPGPPREEDLFEL
SLDEIREKGIQQMPHTLRESVEHMLLDKEFLKKGDVFTEEFIQTYKAYKFETEIWPWEGRPHPFEFLTTYSC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=407, Percent_Identity=25.7985257985258, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1790301, Length=470, Percent_Identity=51.4893617021277, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87081870, Length=383, Percent_Identity=26.1096605744125, Blast_Score=92, Evalue=1e-19,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 53150; Mature: 53018

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00181 GLNA_ATP ; PS00213 LIPOCALIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTPSEVIAYAKANNVLFYDFRFTDIKGAWHHVSYHTIAITEDSFKGLPFDGSSIPAWQ
CCCCHHHHEEEEECCCEEEEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCC
PIDRSDMQLIPDTDAIFLDPFTADPTLVVFCDVFDIYKGTLYEKCPRSIAKKALKYLESS
CCCCCCCEECCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLADTAYFGPENEFFIFDNIKVRDAINVQYYEIDSSEGIWNSHTDFPGSTNTGHRPGTKG
CCCCEEEECCCCCEEEEECEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDSQVDLRVAIVKTLHQIGMETFVVHHEVAQGQGEIGVKFGTLIEAADNVQKL
CCEECCCCCCCCCHHHHHHHHHHHHCHHHEEEHHHHHCCCCCEEEEHHHHHHHHHHHHHH
KYVVKMVAHNYGKTATFMPKPLYGDNGNGMHCHQSIWKNGVNLFAGKGYQNLSDTAMNYI
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
GGVLSHAKACAAFTNASTNSYKRLLPGFEAPSILAYSAQNRSASCRIPFVNGDKARRVEF
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEE
RFPDSSSNPYLAFAAMLMAGIDGVQKKIDPGPPREEDLFELSLDEIREKGIQQMPHTLRE
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVEHMLLDKEFLKKGDVFTEEFIQTYKAYKFETEIWPWEGRPHPFEFLTTYSC
HHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCEECCCCCCCCHHHEEECCC
>Mature Secondary Structure 
SRTPSEVIAYAKANNVLFYDFRFTDIKGAWHHVSYHTIAITEDSFKGLPFDGSSIPAWQ
CCCHHHHEEEEECCCEEEEEEEEEECCCCEEEEEEEEEEEECCCCCCCCCCCCCCCCCC
PIDRSDMQLIPDTDAIFLDPFTADPTLVVFCDVFDIYKGTLYEKCPRSIAKKALKYLESS
CCCCCCCEECCCCCEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLADTAYFGPENEFFIFDNIKVRDAINVQYYEIDSSEGIWNSHTDFPGSTNTGHRPGTKG
CCCCEEEECCCCCEEEEECEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCC
GYFPVAPVDSQVDLRVAIVKTLHQIGMETFVVHHEVAQGQGEIGVKFGTLIEAADNVQKL
CCEECCCCCCCCCHHHHHHHHHHHHCHHHEEEHHHHHCCCCCEEEEHHHHHHHHHHHHHH
KYVVKMVAHNYGKTATFMPKPLYGDNGNGMHCHQSIWKNGVNLFAGKGYQNLSDTAMNYI
HHHHHHHHHCCCCEEEECCCCCCCCCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHH
GGVLSHAKACAAFTNASTNSYKRLLPGFEAPSILAYSAQNRSASCRIPFVNGDKARRVEF
HHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEECCCCCCEEEEEEECCCCCEEEEE
RFPDSSSNPYLAFAAMLMAGIDGVQKKIDPGPPREEDLFELSLDEIREKGIQQMPHTLRE
ECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
SVEHMLLDKEFLKKGDVFTEEFIQTYKAYKFETEIWPWEGRPHPFEFLTTYSC
HHHHHHHHHHHHHCCCCHHHHHHHHHHHEEEECCEECCCCCCCCHHHEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]