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The map label for this gene is kinE [H]

Identifier: 116328545

GI number: 116328545

Start: 2212915

End: 2214036

Strand: Direct

Name: kinE [H]

Synonym: LBL_1899

Alternate gene names: 116328545

Gene position: 2212915-2214036 (Clockwise)

Preceding gene: 116328544

Following gene: 116328546

Centisome position: 61.22

GC content: 38.5

Gene sequence:

>1122_bases
ATGCCTTATGATTTTTTCGAACATTTCCCGTTACCGGCCTGTATCTTAGAGCCGACTCTTTTAATCCAAAAAGTAAACTT
TGCTTTTTGTAGAACTCTAGGTTATTCAAAAACGGAACTAGAGGGAATTCAAAACTGGAATCAAATTTGTCGGTTTAAAG
ACACTACATTCCATTTTTCCGATCCTTCTCTTTGGCCTTGTACGAACTTTTCGAATGAAAATCCCGCCCATTCTGTTCTT
TTTATTACAAAAGACGGAGTTCAAAAGGAATATTCGGTTTCTTTTGCTTTCGATCGTGAAGAATTGCGAATTTTCGCTTA
TTTGGAAACCCCTCAGATTTTTGAGAGGACTACCGTAAAATTCGATTCTCAAAGAGCTTCTGTAGTGGATAAGGATTGGG
AAAAACCGAGCGCTTCTTGGGTTCAAAAGGAAAAATTAGACGCAATCGGAACTCTTTCCGCGGGTGTTGCGCATGAAATT
AATAATCCATTGATGGGTGTGATCAATTATGCGGAAATGATGTTTAACGGTATCGAAAACGGAAATCCTCTTCGTAAATA
TGCGGGAATTATTCTACGAGAAGGAAATCGAATTTCCGAGATCGTGAAAGATATGCTTACCTTCACTCGAGTCGAAAAGG
AAGAAATAAAAATCCAAGATATTAAATCCGTTTTCGATTCCGCGCTCGGCGTGATAAAAGCTTGTATCGATAAGTCGAAA
ATAGAAACGGTAATACCTGATCTAAAAATTCCTCTCTTTGCGGCTTGTTCCGCCGGAAAACTCAGAAAAGTGTATTTCAA
TCTGCTTACAAATGCAAAAGATGCGATCGAAAGCAATTCCGATCCAGGTCAAAAAAGAATAATTGCCGTTCGTTGGGAGA
AATTAAGAAAAGAAAATCGAAATTTCGTTCGTACGATCATTGAAGATACTGGAATCGGTATTCCGGAAGAAAACAGACAT
AAACTTTTCGATCCTTTTTATACGACAAAAGACATCGGAAAAGGCACGGGTCTTGGACTTACGGTTTCCTATAACTTAGT
TCGCGAAATGAAAGGAGACATTTCTTTCGAAAGTGGAAACGGAATCACACGTTTTTATTTGGATCTTCCCGAATCGCTTT
GA

Upstream 100 bases:

>100_bases
ACAGATTTTACCGGATTCTGACATTGGATTTGGTCAGCTTTCCGGTTCTAATCTATCGTAAAAAGGAGGAGCCTTCCGAT
TGCGATAGCTTTATAACCAA

Downstream 100 bases:

>100_bases
AAGAAAGATTCTTAACTTTATGGGAGACGGTCTACTAGATTCTTCTTCAATTTGCCATGGACGGCTCCCGACAATCAGAA
CAGAAAGGCCACGGCGATCC

Product: sensor histidine kinase of a two component response regulator

Products: NA

Alternate protein names: Sensor histidine kinase E [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MPYDFFEHFPLPACILEPTLLIQKVNFAFCRTLGYSKTELEGIQNWNQICRFKDTTFHFSDPSLWPCTNFSNENPAHSVL
FITKDGVQKEYSVSFAFDREELRIFAYLETPQIFERTTVKFDSQRASVVDKDWEKPSASWVQKEKLDAIGTLSAGVAHEI
NNPLMGVINYAEMMFNGIENGNPLRKYAGIILREGNRISEIVKDMLTFTRVEKEEIKIQDIKSVFDSALGVIKACIDKSK
IETVIPDLKIPLFAACSAGKLRKVYFNLLTNAKDAIESNSDPGQKRIIAVRWEKLRKENRNFVRTIIEDTGIGIPEENRH
KLFDPFYTTKDIGKGTGLGLTVSYNLVREMKGDISFESGNGITRFYLDLPESL

Sequences:

>Translated_373_residues
MPYDFFEHFPLPACILEPTLLIQKVNFAFCRTLGYSKTELEGIQNWNQICRFKDTTFHFSDPSLWPCTNFSNENPAHSVL
FITKDGVQKEYSVSFAFDREELRIFAYLETPQIFERTTVKFDSQRASVVDKDWEKPSASWVQKEKLDAIGTLSAGVAHEI
NNPLMGVINYAEMMFNGIENGNPLRKYAGIILREGNRISEIVKDMLTFTRVEKEEIKIQDIKSVFDSALGVIKACIDKSK
IETVIPDLKIPLFAACSAGKLRKVYFNLLTNAKDAIESNSDPGQKRIIAVRWEKLRKENRNFVRTIIEDTGIGIPEENRH
KLFDPFYTTKDIGKGTGLGLTVSYNLVREMKGDISFESGNGITRFYLDLPESL
>Mature_372_residues
PYDFFEHFPLPACILEPTLLIQKVNFAFCRTLGYSKTELEGIQNWNQICRFKDTTFHFSDPSLWPCTNFSNENPAHSVLF
ITKDGVQKEYSVSFAFDREELRIFAYLETPQIFERTTVKFDSQRASVVDKDWEKPSASWVQKEKLDAIGTLSAGVAHEIN
NPLMGVINYAEMMFNGIENGNPLRKYAGIILREGNRISEIVKDMLTFTRVEKEEIKIQDIKSVFDSALGVIKACIDKSKI
ETVIPDLKIPLFAACSAGKLRKVYFNLLTNAKDAIESNSDPGQKRIIAVRWEKLRKENRNFVRTIIEDTGIGIPEENRHK
LFDPFYTTKDIGKGTGLGLTVSYNLVREMKGDISFESGNGITRFYLDLPESL

Specific function: Phosphorylates the sporulation-regulatory protein spo0A under biofilm growth conditions. Also able to weakly phosphorylate spo0F [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 PAS (PER-ARNT-SIM) domains [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=342, Percent_Identity=26.9005847953216, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790436, Length=237, Percent_Identity=29.535864978903, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789149, Length=222, Percent_Identity=30.6306306306306, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994928, Length=221, Percent_Identity=26.6968325791855, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI145693157, Length=229, Percent_Identity=24.8908296943231, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790300, Length=247, Percent_Identity=27.1255060728745, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1786783, Length=230, Percent_Identity=26.9565217391304, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42543; Mature: 42412

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYDFFEHFPLPACILEPTLLIQKVNFAFCRTLGYSKTELEGIQNWNQICRFKDTTFHFS
CCCCHHHHCCCCHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEC
DPSLWPCTNFSNENPAHSVLFITKDGVQKEYSVSFAFDREELRIFAYLETPQIFERTTVK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCHHHHHHHEE
FDSQRASVVDKDWEKPSASWVQKEKLDAIGTLSAGVAHEINNPLMGVINYAEMMFNGIEN
ECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GNPLRKYAGIILREGNRISEIVKDMLTFTRVEKEEIKIQDIKSVFDSALGVIKACIDKSK
CCHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IETVIPDLKIPLFAACSAGKLRKVYFNLLTNAKDAIESNSDPGQKRIIAVRWEKLRKENR
HHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCCCHHEEEEHHHHHHHHHCH
NFVRTIIEDTGIGIPEENRHKLFDPFYTTKDIGKGTGLGLTVSYNLVREMKGDISFESGN
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEHHHHHHHCCCEEEECCC
GITRFYLDLPESL
CEEEEEEECCCCC
>Mature Secondary Structure 
PYDFFEHFPLPACILEPTLLIQKVNFAFCRTLGYSKTELEGIQNWNQICRFKDTTFHFS
CCCHHHHCCCCHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEC
DPSLWPCTNFSNENPAHSVLFITKDGVQKEYSVSFAFDREELRIFAYLETPQIFERTTVK
CCCCCCCCCCCCCCCCCEEEEEECCCCCCEEEEEEEECCCCEEEEEEECCCHHHHHHHEE
FDSQRASVVDKDWEKPSASWVQKEKLDAIGTLSAGVAHEINNPLMGVINYAEMMFNGIEN
ECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCC
GNPLRKYAGIILREGNRISEIVKDMLTFTRVEKEEIKIQDIKSVFDSALGVIKACIDKSK
CCHHHHHHCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IETVIPDLKIPLFAACSAGKLRKVYFNLLTNAKDAIESNSDPGQKRIIAVRWEKLRKENR
HHHHCCCCCCCHHHHCCCCHHHHHHHHHHHCHHHHHCCCCCCCHHEEEEHHHHHHHHHCH
NFVRTIIEDTGIGIPEENRHKLFDPFYTTKDIGKGTGLGLTVSYNLVREMKGDISFESGN
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEEEHHHHHHHCCCEEEECCC
GITRFYLDLPESL
CEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]