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The map label for this gene is gatA

Identifier: 116328527

GI number: 116328527

Start: 2190213

End: 2191670

Strand: Reverse

Name: gatA

Synonym: LBL_1879

Alternate gene names: 116328527

Gene position: 2191670-2190213 (Counterclockwise)

Preceding gene: 116328528

Following gene: 116328526

Centisome position: 60.64

GC content: 45.47

Gene sequence:

>1458_bases
ATGAACGAAATATTAAGAAAATCTTATGCTGAGCTTAAATCCTCCTTAAGCTTGGGAAAAATTTCTGCGACCGAACTTGC
AACGGCTTGTATCGAAAGAATTAAGGAAGTGGACGGATCTGTGAAAGCTTTCCTTTCTTTAGATGAAAAAAGAATTTTGG
ATGCCGCTTCGGAAAGCGACGCGCGCAGAAAGTCGGGGAACCCTCTTTCCGAATTTGACGGAATGCCGATTGCAATCAAA
GACAATATTTGTATCCGGGACTCGATCACTTCCTGCGCCTCAAAAATATTAGAAAATTATAAATCTCCGTTTCATGCGAC
CGTGATCGAAAAACTGATCGCAAAGGGATTCGTACTGATTCCGAGAGCGAATATGGACGAATTTGCGATGGGAAGTTCCA
CGGAAAATTCCGCGTTTCAGACGACTCGAAATCCATTTGACTTGGAGAGAATTCCGGGCGGTTCGAGCGGTGGCTCGGCG
GCGGCAGTTGCAGCTTCGATGGTGCCATTGGCTCTCGGTTCCGACACGGGAGGATCGGTTCGTCAACCCGCTTCTCTTTG
CGGGCTATACGGACTCAAACCTACTTATGGAACGGTTTCCCGTTACGGATTGGTAGCCTATGCTTCCAGTCTCGATCAGA
TCGGGCCTTTTTCAAAAGAATTACAAGGTTGTATCGATCTGTATTCGGTGATTTCCGGAAAGGACGAAAGGGATTCTACG
TCTCTCAATCATCCCGGTTTTTCCGCCCCTTGGACTCCCGATTTCAAAGGGTTAAAAATCGGTACAATCAAAATGACTTC
GGAGATACAACCCGAAGTCGTAAAGGCATATGAAAAAGTCCTCAATCAGTTGAAGGAAAAAGGGGCTACATTGGTTGAAC
TTGATTTTTCCAAATTCGATTTTGCGATCCCGATCTATTACATCATTGCCACAGCGGAATGTTCTTCCAATCTTTCCCGT
TTTGACGGGATTCGTTTCGGATCTAGAAAGGACAAAACCGGAAAACTCGAAGACCTTTTTGTCGATTCTAGAACGACAGG
ATTTGGTTCCGAAGTAAAACGAAGAATTTTACTCGGGACTTTTTCCCTTTCCGCGGGCTACTACGACGCGTATTACGGAA
CCGCACAGAAGGCAAGGGTACTCATTCGTAAAGAATACGACTCGTTTTTCTCCAAAGTCGATTTTATCTTGCAGCCTACT
TCTCCGACGACCGCTTTTAAGGTCGGAGAAAAAACGAAAGACCCGATTCAGATGTATAAGGCGGATATCTGGACCACGAG
CGTGAATCTGGCGGGAATTCCCGCAATCAGTGTTCCGATGGGAACGGACGAGAAGGGACTTCCGATCGGATTACAAATCA
CCGCTCCTCATTTCCAAGAAGGAAAACTTTTCGGAGCGGCTCAAGCCATTGGTGCATTAGAAGATTTGAATATTAAATTT
CCGGAAAATATTGGATGA

Upstream 100 bases:

>100_bases
GTCCGGATCTTGTGGGTCAACATCTTTCTCGGGAAGAAATCGAAAAGTTTGCGCCTTCCTTTCAAAACGGATATTTCGTC
GTCCCTAAGGTAATCGAAAC

Downstream 100 bases:

>100_bases
GTAATTTAACGGCAAGAGTCATCCCCTGCCTGGACATCAAGGATGGACGGGTAGTAAAGGGGGTTAACTTTGTCGACCTT
GTGGATGCGGGAGATCCTGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MNEILRKSYAELKSSLSLGKISATELATACIERIKEVDGSVKAFLSLDEKRILDAASESDARRKSGNPLSEFDGMPIAIK
DNICIRDSITSCASKILENYKSPFHATVIEKLIAKGFVLIPRANMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSA
AAVAASMVPLALGSDTGGSVRQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSKELQGCIDLYSVISGKDERDST
SLNHPGFSAPWTPDFKGLKIGTIKMTSEIQPEVVKAYEKVLNQLKEKGATLVELDFSKFDFAIPIYYIIATAECSSNLSR
FDGIRFGSRKDKTGKLEDLFVDSRTTGFGSEVKRRILLGTFSLSAGYYDAYYGTAQKARVLIRKEYDSFFSKVDFILQPT
SPTTAFKVGEKTKDPIQMYKADIWTTSVNLAGIPAISVPMGTDEKGLPIGLQITAPHFQEGKLFGAAQAIGALEDLNIKF
PENIG

Sequences:

>Translated_485_residues
MNEILRKSYAELKSSLSLGKISATELATACIERIKEVDGSVKAFLSLDEKRILDAASESDARRKSGNPLSEFDGMPIAIK
DNICIRDSITSCASKILENYKSPFHATVIEKLIAKGFVLIPRANMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSA
AAVAASMVPLALGSDTGGSVRQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSKELQGCIDLYSVISGKDERDST
SLNHPGFSAPWTPDFKGLKIGTIKMTSEIQPEVVKAYEKVLNQLKEKGATLVELDFSKFDFAIPIYYIIATAECSSNLSR
FDGIRFGSRKDKTGKLEDLFVDSRTTGFGSEVKRRILLGTFSLSAGYYDAYYGTAQKARVLIRKEYDSFFSKVDFILQPT
SPTTAFKVGEKTKDPIQMYKADIWTTSVNLAGIPAISVPMGTDEKGLPIGLQITAPHFQEGKLFGAAQAIGALEDLNIKF
PENIG
>Mature_485_residues
MNEILRKSYAELKSSLSLGKISATELATACIERIKEVDGSVKAFLSLDEKRILDAASESDARRKSGNPLSEFDGMPIAIK
DNICIRDSITSCASKILENYKSPFHATVIEKLIAKGFVLIPRANMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSA
AAVAASMVPLALGSDTGGSVRQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSKELQGCIDLYSVISGKDERDST
SLNHPGFSAPWTPDFKGLKIGTIKMTSEIQPEVVKAYEKVLNQLKEKGATLVELDFSKFDFAIPIYYIIATAECSSNLSR
FDGIRFGSRKDKTGKLEDLFVDSRTTGFGSEVKRRILLGTFSLSAGYYDAYYGTAQKARVLIRKEYDSFFSKVDFILQPT
SPTTAFKVGEKTKDPIQMYKADIWTTSVNLAGIPAISVPMGTDEKGLPIGLQITAPHFQEGKLFGAAQAIGALEDLNIKF
PENIG

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=33.9920948616601, Blast_Score=291, Evalue=1e-78,
Organism=Homo sapiens, GI195972892, Length=489, Percent_Identity=23.3128834355828, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17543272, Length=428, Percent_Identity=36.9158878504673, Blast_Score=265, Evalue=4e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=498, Percent_Identity=24.4979919678715, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71990152, Length=322, Percent_Identity=28.2608695652174, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=192, Percent_Identity=28.125, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=315, Percent_Identity=26.984126984127, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556276, Length=195, Percent_Identity=32.3076923076923, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=196, Percent_Identity=28.5714285714286, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=35.2803738317757, Blast_Score=216, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6319685, Length=432, Percent_Identity=29.1666666666667, Blast_Score=110, Evalue=6e-25,
Organism=Saccharomyces cerevisiae, GI6320448, Length=523, Percent_Identity=20.6500956022945, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648113, Length=496, Percent_Identity=36.0887096774194, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24644968, Length=489, Percent_Identity=25.3578732106339, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24652985, Length=486, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI19922090, Length=486, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652981, Length=486, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24652983, Length=486, Percent_Identity=25.9259259259259, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161078093, Length=491, Percent_Identity=24.0325865580448, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI45550774, Length=213, Percent_Identity=31.4553990610329, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648435, Length=213, Percent_Identity=31.4553990610329, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24648437, Length=213, Percent_Identity=31.4553990610329, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI21356731, Length=241, Percent_Identity=28.2157676348548, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=36.6906474820144, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=36.6906474820144, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATA_LEPBJ (Q04SA5)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800973.1
- ProteinModelPortal:   Q04SA5
- SMR:   Q04SA5
- STRING:   Q04SA5
- GeneID:   4411050
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1660
- eggNOG:   COG0154
- HOGENOM:   HBG481888
- OMA:   TESSCYG
- PhylomeDB:   Q04SA5
- ProtClustDB:   PRK00012
- BioCyc:   LBOR355277:LBJ_1660-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00120
- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412
- Gene3D:   G3DSA:3.90.1300.10
- PANTHER:   PTHR11895
- TIGRFAMs:   TIGR00132

Pfam domain/function: PF01425 Amidase; SSF75304 Amidase_sig_enz

EC number: 6.3.5.-

Molecular weight: Translated: 52577; Mature: 52577

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: PS00571 AMIDASES

Important sites: ACT_SITE 80-80 ACT_SITE 155-155 ACT_SITE 179-179

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEILRKSYAELKSSLSLGKISATELATACIERIKEVDGSVKAFLSLDEKRILDAASESD
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCHH
ARRKSGNPLSEFDGMPIAIKDNICIRDSITSCASKILENYKSPFHATVIEKLIAKGFVLI
HHHHCCCCHHHHCCCCEEEECCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
PRANMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSAAAVAASMVPLALGSDTGGSV
ECCCCHHHHCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCCC
RQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSKELQGCIDLYSVISGKDERDST
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
SLNHPGFSAPWTPDFKGLKIGTIKMTSEIQPEVVKAYEKVLNQLKEKGATLVELDFSKFD
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
FAIPIYYIIATAECSSNLSRFDGIRFGSRKDKTGKLEDLFVDSRTTGFGSEVKRRILLGT
HHEEEEEEEEEHHHHCCHHHHCCCCCCCCCCCCCCHHHEEECCCCCCCHHHHHHHHHEEE
FSLSAGYYDAYYGTAQKARVLIRKEYDSFFSKVDFILQPTSPTTAFKVGEKTKDPIQMYK
EECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCCCCCHHHHHH
ADIWTTSVNLAGIPAISVPMGTDEKGLPIGLQITAPHFQEGKLFGAAQAIGALEDLNIKF
HHHEEEEECCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
PENIG
CCCCC
>Mature Secondary Structure
MNEILRKSYAELKSSLSLGKISATELATACIERIKEVDGSVKAFLSLDEKRILDAASESD
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCHH
ARRKSGNPLSEFDGMPIAIKDNICIRDSITSCASKILENYKSPFHATVIEKLIAKGFVLI
HHHHCCCCHHHHCCCCEEEECCEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
PRANMDEFAMGSSTENSAFQTTRNPFDLERIPGGSSGGSAAAVAASMVPLALGSDTGGSV
ECCCCHHHHCCCCCCCCCHHCCCCCCCHHCCCCCCCCCCHHHHHHHHHHHEECCCCCCCC
RQPASLCGLYGLKPTYGTVSRYGLVAYASSLDQIGPFSKELQGCIDLYSVISGKDERDST
CCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
SLNHPGFSAPWTPDFKGLKIGTIKMTSEIQPEVVKAYEKVLNQLKEKGATLVELDFSKFD
CCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
FAIPIYYIIATAECSSNLSRFDGIRFGSRKDKTGKLEDLFVDSRTTGFGSEVKRRILLGT
HHEEEEEEEEEHHHHCCHHHHCCCCCCCCCCCCCCHHHEEECCCCCCCHHHHHHHHHEEE
FSLSAGYYDAYYGTAQKARVLIRKEYDSFFSKVDFILQPTSPTTAFKVGEKTKDPIQMYK
EECCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHEEEECCCCCCEEECCCCCCCHHHHHH
ADIWTTSVNLAGIPAISVPMGTDEKGLPIGLQITAPHFQEGKLFGAAQAIGALEDLNIKF
HHHEEEEECCCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCC
PENIG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA