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The map label for this gene is hisF-1 [H]

Identifier: 116328526

GI number: 116328526

Start: 2189446

End: 2190216

Strand: Reverse

Name: hisF-1 [H]

Synonym: LBL_1878

Alternate gene names: 116328526

Gene position: 2190216-2189446 (Counterclockwise)

Preceding gene: 116328527

Following gene: 116328519

Centisome position: 60.6

GC content: 47.34

Gene sequence:

>771_bases
ATGAGTAATTTAACGGCAAGAGTCATCCCCTGCCTGGACATCAAGGATGGACGGGTAGTAAAGGGGGTTAACTTTGTCGA
CCTTGTGGATGCGGGAGATCCTGTGGAATCCGCTGGGATCTACGAGGAAAACTTAGCGGACGAACTCTGCTTTTTAGATA
TCACCGCGTCTTCCGACCGAAGGGAGATTCTGCTGCATCTTGTGGAAAGAATCGCCGAGAAAATTTTCATTCCTTTTACG
GTAGGAGGTGGAATCCGAACTGTAGCCGATGTCAAAGCTGTTTTGGAAAAAGGAGCGGATAAGATTTCGATCAACACCGC
TGCTTTTCAGAATCCGGAGCTTTTAACGCATTCGTCCGAAATATACGGGTCCCAATGTATCGTTTGCGCGATCGATGTGA
AGTTTCAGAAAGAAAGAGATCGATATGAGATCTTTTTACACGGAGGACGAACGGAAACCGGGAGAGAGGCGCTTGATTGG
GCTCGGGAAGCTGTCGGAAGAGGAGCTGGTGAAATTTTACTTACGTCTATGGATCGGGACGGAACTAGAAACGGATTCGA
TATCAACCTTCTAAAAAGTTTTTCTTCGTCCCTTGAAATACCGATTATCGCTTCGGGTGGGGCGGGCAATCCGGAACACA
TGGTGGAAGCGATCTTAAGAGGAAAGGCGGATGCAGTTCTTGCGGCTTCCATATTTCATTTCGGAGAATATTCCATCCGT
GAAACGAAAAGAGCCATGCAGGAAATGGGAATTTCCGTTCGACTGGATTGA

Upstream 100 bases:

>100_bases
AAATCACCGCTCCTCATTTCCAAGAAGGAAAACTTTTCGGAGCGGCTCAAGCCATTGGTGCATTAGAAGATTTGAATATT
AAATTTCCGGAAAATATTGG

Downstream 100 bases:

>100_bases
AGAAATGAACATTTATCTCTAAAAGTTATACGCTGAAAGTAACTTTTCAGTTGTGTTACTTTTTACTAATTTAACGTGAG
TTCGATATAACAAAATGCGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MSNLTARVIPCLDIKDGRVVKGVNFVDLVDAGDPVESAGIYEENLADELCFLDITASSDRREILLHLVERIAEKIFIPFT
VGGGIRTVADVKAVLEKGADKISINTAAFQNPELLTHSSEIYGSQCIVCAIDVKFQKERDRYEIFLHGGRTETGREALDW
AREAVGRGAGEILLTSMDRDGTRNGFDINLLKSFSSSLEIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
ETKRAMQEMGISVRLD

Sequences:

>Translated_256_residues
MSNLTARVIPCLDIKDGRVVKGVNFVDLVDAGDPVESAGIYEENLADELCFLDITASSDRREILLHLVERIAEKIFIPFT
VGGGIRTVADVKAVLEKGADKISINTAAFQNPELLTHSSEIYGSQCIVCAIDVKFQKERDRYEIFLHGGRTETGREALDW
AREAVGRGAGEILLTSMDRDGTRNGFDINLLKSFSSSLEIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
ETKRAMQEMGISVRLD
>Mature_255_residues
SNLTARVIPCLDIKDGRVVKGVNFVDLVDAGDPVESAGIYEENLADELCFLDITASSDRREILLHLVERIAEKIFIPFTV
GGGIRTVADVKAVLEKGADKISINTAAFQNPELLTHSSEIYGSQCIVCAIDVKFQKERDRYEIFLHGGRTETGREALDWA
REAVGRGAGEILLTSMDRDGTRNGFDINLLKSFSSSLEIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIRE
TKRAMQEMGISVRLD

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=256, Percent_Identity=46.09375, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=24.8979591836735, Blast_Score=82, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=32.6984126984127, Blast_Score=151, Evalue=9e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.-

Molecular weight: Translated: 27972; Mature: 27841

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLTARVIPCLDIKDGRVVKGVNFVDLVDAGDPVESAGIYEENLADELCFLDITASSDR
CCCCCEEEEEEEECCCCCEEECCCEEEEECCCCCCHHCCCCHHHCCCCEEEEEEECCCCH
REILLHLVERIAEKIFIPFTVGGGIRTVADVKAVLEKGADKISINTAAFQNPELLTHSSE
HHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCEECCHH
IYGSQCIVCAIDVKFQKERDRYEIFLHGGRTETGREALDWAREAVGRGAGEILLTSMDRD
HCCCEEEEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
GTRNGFDINLLKSFSSSLEIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
ETKRAMQEMGISVRLD
HHHHHHHHCCCEEEEC
>Mature Secondary Structure 
SNLTARVIPCLDIKDGRVVKGVNFVDLVDAGDPVESAGIYEENLADELCFLDITASSDR
CCCCEEEEEEEECCCCCEEECCCEEEEECCCCCCHHCCCCHHHCCCCEEEEEEECCCCH
REILLHLVERIAEKIFIPFTVGGGIRTVADVKAVLEKGADKISINTAAFQNPELLTHSSE
HHHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCCCEECCHH
IYGSQCIVCAIDVKFQKERDRYEIFLHGGRTETGREALDWAREAVGRGAGEILLTSMDRD
HCCCEEEEEEEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCC
GTRNGFDINLLKSFSSSLEIPIIASGGAGNPEHMVEAILRGKADAVLAASIFHFGEYSIR
CCCCCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCCCHHH
ETKRAMQEMGISVRLD
HHHHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12712204 [H]