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The map label for this gene is metK

Identifier: 116328514

GI number: 116328514

Start: 2171554

End: 2172714

Strand: Reverse

Name: metK

Synonym: LBL_1864

Alternate gene names: 116328514

Gene position: 2172714-2171554 (Counterclockwise)

Preceding gene: 116328518

Following gene: 116328513

Centisome position: 60.11

GC content: 45.22

Gene sequence:

>1161_bases
ATGTCTTTGAAAGATTTTATTTTTACTTCGGAATCAGTTGGTGAAGGCCACCCGGACAAGGTATGCGACCAAATTTCCGA
TGCGATTTTGGACGCATACCTCGAACAAGATCCGAAATCCAGAGTTGCTTGCGAAACCTTAGCGACCACAAACTTGGTCG
TGATTGCAGGTGAAATTACAAGTAAGGGAAAGGTAGACGCTCAAGAAATTGCAAGAAACGTAATTCGTGATATCGGTTAC
AATGATATTACCATGGGTTTCGACGCGGACTTTGCCGTCGTTTCCGCTCACGTTCATGCTCAAAGTCCGGACATCTCTCA
AGGGGTTACCGAAGGAGAAGGTCTTTTCAAAGAGCAGGGAGCAGGGGATCAGGGTTTGATGTTCGGTTTTGCGATTAATG
AAACTCCGGAATTCATGCCGATGCCGATTTATTATTCTCACGAGTTGGTAAAACACTTAGCCGGACTCAGACATTCTAAC
AAATTGAAATTCTTAAGACCGGATGCTAAATCTCAGGTTACGGTAGAATACAAAGACGGGAAACCGGTTCGTGTAGATAC
CGTCGTTATCTCCACCCAACATTCTCCCGACGTGGCTCATAAACAAATCGAAGAATCTCTCATCGAAGAATGTATTAAGA
AAGTAATTCCGGCAAATCTTCTCGTTAATACGAAGTATTTCATCAACCCGACCGGTCAATTCATCGTAGGCGGTCCTCAT
GGAGACGCGGGCCTTACTGGAAGAAAGATCATTGTAGATACGTACGGTGGATACGGAAGGCACGGGGGGGGAGCGTTCTC
CGGTAAGGATCCTTCCAAAGTGGACCGTTCTGCCGCTTATATGGGACGTTATATCGCAAAGAACGTAGTTGCTTCCGGTC
TTGCGGACAAATGCGAAGTACAACTTGCATATGCGATCGGGGTTGCGGAACCGGTTTCGGTTCACGTGGACACATTCGGA
ACAGGAAAAATCTCCGAGGAAGAATTGGTCAAAAGAATCCGTGCAAACTTCAAGCTAACTCCGAGAGGAATCATCGAATC
TCTGAAACTTCTCGAAAAAGGAAGAAAGTATAGAGAGACTGCATCGTATGGACATTTCGGAAGAAAAGGGTCCACATTCA
CTTGGGAAAAAACCGATAAAACGGTAGCTTTAAAAGGATAA

Upstream 100 bases:

>100_bases
AAAGGAAGATGTCTTGGATCTTCTTGACACTGAAGGAATCCGTAGGTTTTTGGGATCAGAGCGGGTCTTCCCGTCAATTT
TCACCTATCAGGAACAACAG

Downstream 100 bases:

>100_bases
GAAAAAATGGGAGCTCCAAGCACATCGACTGCGACAGAAAAAGCGACTCGTGACGGTTATGGAGATGCACTGCATGAGCT
GGGAGTTTCCCGTCAGGATG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSLKDFIFTSESVGEGHPDKVCDQISDAILDAYLEQDPKSRVACETLATTNLVVIAGEITSKGKVDAQEIARNVIRDIGY
NDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQGAGDQGLMFGFAINETPEFMPMPIYYSHELVKHLAGLRHSN
KLKFLRPDAKSQVTVEYKDGKPVRVDTVVISTQHSPDVAHKQIEESLIEECIKKVIPANLLVNTKYFINPTGQFIVGGPH
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEVQLAYAIGVAEPVSVHVDTFG
TGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRETASYGHFGRKGSTFTWEKTDKTVALKG

Sequences:

>Translated_386_residues
MSLKDFIFTSESVGEGHPDKVCDQISDAILDAYLEQDPKSRVACETLATTNLVVIAGEITSKGKVDAQEIARNVIRDIGY
NDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQGAGDQGLMFGFAINETPEFMPMPIYYSHELVKHLAGLRHSN
KLKFLRPDAKSQVTVEYKDGKPVRVDTVVISTQHSPDVAHKQIEESLIEECIKKVIPANLLVNTKYFINPTGQFIVGGPH
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEVQLAYAIGVAEPVSVHVDTFG
TGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRETASYGHFGRKGSTFTWEKTDKTVALKG
>Mature_385_residues
SLKDFIFTSESVGEGHPDKVCDQISDAILDAYLEQDPKSRVACETLATTNLVVIAGEITSKGKVDAQEIARNVIRDIGYN
DITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQGAGDQGLMFGFAINETPEFMPMPIYYSHELVKHLAGLRHSNK
LKFLRPDAKSQVTVEYKDGKPVRVDTVVISTQHSPDVAHKQIEESLIEECIKKVIPANLLVNTKYFINPTGQFIVGGPHG
DAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEVQLAYAIGVAEPVSVHVDTFGT
GKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRETASYGHFGRKGSTFTWEKTDKTVALKG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=376, Percent_Identity=52.6595744680851, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=52.3560209424084, Blast_Score=374, Evalue=1e-103,
Organism=Escherichia coli, GI1789311, Length=380, Percent_Identity=60.5263157894737, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=55.0649350649351, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538494, Length=382, Percent_Identity=55.4973821989529, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=382, Percent_Identity=54.9738219895288, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=54.5454545454545, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=50, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI25145635, Length=334, Percent_Identity=52.9940119760479, Blast_Score=316, Evalue=1e-86,
Organism=Caenorhabditis elegans, GI32565911, Length=331, Percent_Identity=53.1722054380665, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=53.2894736842105, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=60.9195402298851, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=72.7272727272727, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320710, Length=382, Percent_Identity=53.1413612565445, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6323209, Length=377, Percent_Identity=54.1114058355438, Blast_Score=393, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=51.9893899204244, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=51.9893899204244, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=51.9893899204244, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=51.9893899204244, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=51.9893899204244, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=51.9893899204244, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=51.3157894736842, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=51.3157894736842, Blast_Score=356, Evalue=1e-98,
Organism=Drosophila melanogaster, GI24580541, Length=367, Percent_Identity=50.1362397820163, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=53.2786885245902, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_LEPBJ (Q04SB8)

Other databases:

- EMBL:   CP000350
- RefSeq:   YP_800960.1
- ProteinModelPortal:   Q04SB8
- SMR:   Q04SB8
- STRING:   Q04SB8
- GeneID:   4411036
- GenomeReviews:   CP000350_GR
- KEGG:   lbj:LBJ_1645
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   IYYAHRL
- PhylomeDB:   Q04SB8
- ProtClustDB:   PRK05250
- BioCyc:   LBOR355277:LBJ_1645-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42068; Mature: 41937

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKDFIFTSESVGEGHPDKVCDQISDAILDAYLEQDPKSRVACETLATTNLVVIAGEIT
CCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCC
SKGKVDAQEIARNVIRDIGYNDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQG
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCHHHHCC
AGDQGLMFGFAINETPEFMPMPIYYSHELVKHLAGLRHSNKLKFLRPDAKSQVTVEYKDG
CCCCCEEEEEEECCCCCCEECCEEHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECCC
KPVRVDTVVISTQHSPDVAHKQIEESLIEECIKKVIPANLLVNTKYFINPTGQFIVGGPH
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECEEEECCCCCEEEECCC
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEE
QLAYAIGVAEPVSVHVDTFGTGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRET
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHH
ASYGHFGRKGSTFTWEKTDKTVALKG
HCCCCCCCCCCEEEEECCCCEEEECC
>Mature Secondary Structure 
SLKDFIFTSESVGEGHPDKVCDQISDAILDAYLEQDPKSRVACETLATTNLVVIAGEIT
CCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCC
SKGKVDAQEIARNVIRDIGYNDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQG
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCHHHHCC
AGDQGLMFGFAINETPEFMPMPIYYSHELVKHLAGLRHSNKLKFLRPDAKSQVTVEYKDG
CCCCCEEEEEEECCCCCCEECCEEHHHHHHHHHHCCCCCCCEEEECCCCCCEEEEEECCC
KPVRVDTVVISTQHSPDVAHKQIEESLIEECIKKVIPANLLVNTKYFINPTGQFIVGGPH
CCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECEEEECCCCCEEEECCC
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEE
QLAYAIGVAEPVSVHVDTFGTGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRET
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHH
ASYGHFGRKGSTFTWEKTDKTVALKG
HCCCCCCCCCCEEEEECCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA