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The map label for this gene is thiE [H]

Identifier: 116328410

GI number: 116328410

Start: 2037908

End: 2038558

Strand: Reverse

Name: thiE [H]

Synonym: LBL_1750

Alternate gene names: 116328410

Gene position: 2038558-2037908 (Counterclockwise)

Preceding gene: 116328412

Following gene: 116328409

Centisome position: 56.4

GC content: 40.55

Gene sequence:

>651_bases
TTGTCGCTACAAATTCAAACTCCTGTTTGGCCGTTGCCTGGAATTTATCCGATTCTTGATTGGGATTTTTGTAAAAGGAA
AAATCTCGATCTTTTAACTCTCCCCAGGATTTGGTTGGAATATCCGGATCTCGTTCCTTTTGTTCAAATCCGCGCCAAGT
CGGCTTCTATTCTCGAATTGGAATTTCTTGTTAAGTCCCTTCAGGATCGATATCCTCGCTTACTTTTGATTTTAAACGAT
TTTTGGGAACAAGCGATCGAATGGAATTGTTTTGGCGCTCATGTTGGAAAAGAAGATTATGAGGCTTTGAATTCCGAAGA
AAAGAAAGTTTTATTCGACTCGAAGCTCTATCTCGGAACCTCGTCTCATACGTTGGAGGAAGTGAACGGATTGGATTCTT
CCCTTTGGAATTATACCGGGCTTGGGCCGATTTTTCCTACCGATAATAAAGAAGATGCAAAGCCCGCAATCGGGACGGAA
ATTTTAGGGAAAGTTACAAGGGAATCCCGATTACCTGTAACTTTAATCGGTGGAATCCAAGTTGGAAATTTGGATCTGAT
TTTGAAAAAGGGGGCGTTTTTACTTTCAAGTATCTCTATGGCTTGTTTAGAAAGAGAATTTAGAGCGGCTGCAACGAAAA
TACGAAAGTAA

Upstream 100 bases:

>100_bases
AACTTAGTTCCGGCGTGTTTGCCATTCAAAATAAGTTGGGGATTCTTTTGGGAAAAAATTTGAAGTTAGGCCGATGAGAG
ATTGTTTGGGGTAGTAAAAC

Downstream 100 bases:

>100_bases
AATCGCTAAGTTTCAATTGACTTTGAACTTATGTCTTATAGGATGCCTTTAGATGAAGAAGCCGGATGAAATCGTACATC
TCTCCGCCAAAGACGATAGG

Product: thiamine monophosphate synthase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MSLQIQTPVWPLPGIYPILDWDFCKRKNLDLLTLPRIWLEYPDLVPFVQIRAKSASILELEFLVKSLQDRYPRLLLILND
FWEQAIEWNCFGAHVGKEDYEALNSEEKKVLFDSKLYLGTSSHTLEEVNGLDSSLWNYTGLGPIFPTDNKEDAKPAIGTE
ILGKVTRESRLPVTLIGGIQVGNLDLILKKGAFLLSSISMACLEREFRAAATKIRK

Sequences:

>Translated_216_residues
MSLQIQTPVWPLPGIYPILDWDFCKRKNLDLLTLPRIWLEYPDLVPFVQIRAKSASILELEFLVKSLQDRYPRLLLILND
FWEQAIEWNCFGAHVGKEDYEALNSEEKKVLFDSKLYLGTSSHTLEEVNGLDSSLWNYTGLGPIFPTDNKEDAKPAIGTE
ILGKVTRESRLPVTLIGGIQVGNLDLILKKGAFLLSSISMACLEREFRAAATKIRK
>Mature_215_residues
SLQIQTPVWPLPGIYPILDWDFCKRKNLDLLTLPRIWLEYPDLVPFVQIRAKSASILELEFLVKSLQDRYPRLLLILNDF
WEQAIEWNCFGAHVGKEDYEALNSEEKKVLFDSKLYLGTSSHTLEEVNGLDSSLWNYTGLGPIFPTDNKEDAKPAIGTEI
LGKVTRESRLPVTLIGGIQVGNLDLILKKGAFLLSSISMACLEREFRAAATKIRK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=211, Percent_Identity=27.4881516587678, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 24538; Mature: 24407

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLQIQTPVWPLPGIYPILDWDFCKRKNLDLLTLPRIWLEYPDLVPFVQIRAKSASILEL
CCEEECCCCCCCCCCCCCCCCHHHCCCCCCEEECCHHHHCCCCCCCHHEEECCCCHHHHH
EFLVKSLQDRYPRLLLILNDFWEQAIEWNCFGAHVGKEDYEALNSEEKKVLFDSKLYLGT
HHHHHHHHHCCCEEEHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCEEEECCEEEECC
SSHTLEEVNGLDSSLWNYTGLGPIFPTDNKEDAKPAIGTEILGKVTRESRLPVTLIGGIQ
CCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEE
VGNLDLILKKGAFLLSSISMACLEREFRAAATKIRK
ECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SLQIQTPVWPLPGIYPILDWDFCKRKNLDLLTLPRIWLEYPDLVPFVQIRAKSASILEL
CEEECCCCCCCCCCCCCCCCHHHCCCCCCEEECCHHHHCCCCCCCHHEEECCCCHHHHH
EFLVKSLQDRYPRLLLILNDFWEQAIEWNCFGAHVGKEDYEALNSEEKKVLFDSKLYLGT
HHHHHHHHHCCCEEEHHHHHHHHHHHCCCEEECCCCHHHHHHHCCCCCEEEECCEEEECC
SSHTLEEVNGLDSSLWNYTGLGPIFPTDNKEDAKPAIGTEILGKVTRESRLPVTLIGGIQ
CCHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCEE
VGNLDLILKKGAFLLSSISMACLEREFRAAATKIRK
ECCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA